(Circular) (Six-base) MAPSORT of: pFN21AB5155.seq Check: 2475 from: 1 to: 6238 pFN21AB5155 6238 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 3581 1937 Size: 1542 102 4594 Fragments arranged by size: 4594 1542 102 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4301 5685 5953 1632 Size: 2669 1384 268 1917 Fragments arranged by size: 2669 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 2750 3426 1166 Size: 1584 676 3978 Fragments arranged by size: 3978 1584 676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 2714 Size: 32 6206 AlwNI CAG_nnn'CTG Cuts at: 3981 5553 3981 Size: 1572 4666 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 3230 3547 3773 3925 6034 1493 Size: 1737 317 226 152 2109 1697 Fragments arranged by size: 2109 1737 1697 317 226 152 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 3563 1870 Size: 73 1620 4545 Fragments arranged by size: 4545 1620 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3401 3559 5014 618 Size: 325 217 627 1614 158 1455 1842 Fragments arranged by size: 1842 1614 1455 627 325 217 158 BanII G_rGCy'C Cuts at: 1391 2061 2581 3557 1391 Size: 670 520 976 4072 Fragments arranged by size: 4072 976 670 520 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5267 BbvCI CC'TCA_GC Cuts at: 2852 2852 Size: 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 4849 136 Size: 107 122 71 631 605 502 2675 1525 Fragments arranged by size: 2675 1525 631 605 502 122 107 71 BglII A'GATC_T Cuts at: 2438 3307 6233 2438 Size: 869 2926 2443 Fragments arranged by size: 2926 2443 869 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 4299 5455 1790 Size: 2509 1156 2573 Fragments arranged by size: 2573 2509 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 2748 3969 Size: 416 53 83 399 24 609 722 1221 Cuts at: 3969 4927 442 Size: 958 1753 Fragments arranged by size: 1753 1221 958 722 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 4898 1239 Size: 1415 2244 2579 Fragments arranged by size: 2579 2244 1415 Bpu10I CC'TnA_GC Cuts at: 2852 2852 Size: 6238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4901 882 Size: 617 3402 2219 Fragments arranged by size: 3402 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4045 4427 5062 275 Size: 53 83 186 936 2512 382 635 1451 Fragments arranged by size: 2512 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2244 2274 3193 3223 1178 Size: 30 165 30 841 30 919 30 4193 Fragments arranged by size: 4193 919 841 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2006 2265 3426 1186 Size: 820 259 1161 3998 Fragments arranged by size: 3998 1161 820 259 BseYI C'CCAG_C Cuts at: 2053 2094 5441 5937 2053 Size: 41 3347 496 2354 Fragments arranged by size: 3347 2354 496 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2798 1163 Size: 573 1062 4603 Fragments arranged by size: 4603 1062 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2581 2747 4299 4384 5455 2581 Size: 166 1552 85 1071 3364 Fragments arranged by size: 3364 1552 1071 166 85 BsmI GAATG_Cn' Cuts at: 2498 3749 3842 2498 Size: 1251 93 4894 Fragments arranged by size: 4894 1251 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2581 2747 3557 4299 4384 5455 Size: 399 271 520 166 810 742 85 1071 Cuts at: 5455 1391 Size: 2174 Fragments arranged by size: 2174 1071 810 742 520 399 271 166 85 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2733 3593 844 Size: 1889 860 3489 Fragments arranged by size: 3489 1889 860 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 3186 4733 4907 65 Size: 1183 1458 480 1547 174 1396 Fragments arranged by size: 1547 1458 1396 1183 480 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4882 5069 1700 Size: 110 3072 187 2869 Fragments arranged by size: 3072 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstXI CCAn_nnnn'nTGG Cuts at: 2632 3310 2632 Size: 678 5560 BstYI r'GATC_y Cuts at: 1972 2292 2438 2988 3307 3568 3935 4320 4337 Size: 320 146 550 319 261 367 385 17 Cuts at: 4337 5789 5875 5887 6233 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 550 385 367 346 320 319 261 146 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3005 513 Size: 552 1940 3746 Fragments arranged by size: 3746 1940 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2289 2524 487 Size: 683 56 1063 235 4201 Fragments arranged by size: 4201 1063 683 235 56 BtsI GCAGTG_nn' Cuts at: 2468 2523 2913 3057 3078 3454 3754 4548 4568 Size: 55 390 144 21 376 300 794 20 Cuts at: 4568 2468 Size: 4138 Fragments arranged by size: 4138 794 390 376 300 144 55 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3229 3542 3229 Size: 313 5925 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2443 2703 2899 Size: 54 1425 50 275 18 613 260 196 Cuts at: 2899 3172 3685 4574 6203 8 Size: 273 513 889 1629 43 Fragments arranged by size: 1629 1425 889 613 513 275 273 260 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2882 3240 4168 1353 Size: 1529 358 928 3423 Fragments arranged by size: 3423 1529 928 358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2579 2579 Size: 6238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2654 4301 4898 5685 5953 1239 Size: 393 1022 1647 597 787 268 1524 Fragments arranged by size: 1647 1524 1022 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2417 3010 2417 Size: 593 5645 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 EcoRV GAT'ATC Cuts at: 2377 2377 Size: 6238 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3498 3498 Size: 6238 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 5385 1678 Size: 3707 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2406 2438 3193 3225 4879 Size: 32 135 32 834 32 755 32 1654 Cuts at: 4879 4911 4953 4985 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1654 834 755 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3370 3582 3828 669 Size: 988 281 1432 212 246 3079 Fragments arranged by size: 3079 1432 988 281 246 212 HindIII A'AGCT_T Cuts at: 748 2308 748 Size: 1560 4678 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3156 3837 3156 Size: 681 5557 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 2464 2507 2606 2784 3401 5352 5536 Size: 880 279 43 99 178 617 1951 184 Cuts at: 5536 1305 Size: 2007 Fragments arranged by size: 2007 1951 880 617 279 184 178 99 43 MscI TGG'CCA Cuts at: 10 64 1489 1832 2445 2705 2901 3174 10 Size: 54 1425 343 613 260 196 273 3074 Fragments arranged by size: 3074 1425 613 343 273 260 196 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 2600 2699 2750 3426 4196 4555 Size: 648 1377 57 99 51 676 770 359 Cuts at: 4555 4714 518 Size: 159 2042 Fragments arranged by size: 2042 1377 770 676 648 359 159 99 57 51 MspA1I CmG'CkG Cuts at: 1606 2897 3654 4331 5479 5724 1606 Size: 1291 757 677 1148 245 2120 Fragments arranged by size: 2120 1291 1148 757 677 245 NcoI C'CATG_G Cuts at: 513 1065 3005 513 Size: 552 1940 3746 Fragments arranged by size: 3746 1940 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3061 3596 5141 1132 Size: 97 1832 535 1545 2229 Fragments arranged by size: 2229 1832 1545 535 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 3383 1108 Size: 2275 3963 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2417 3010 2417 Size: 593 5645 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3590 830 Size: 2760 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 2897 2897 Size: 6238 SacI G_AGCT'C Cuts at: 2581 2581 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SanDI GG'GwC_CC Cuts at: 3010 3010 Size: 6238 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 2301 4486 6198 2301 Size: 2185 1712 2341 Fragments arranged by size: 2341 2185 1712 SfcI C'TryA_G Cuts at: 826 1045 3496 3586 4721 5402 5593 826 Size: 219 2451 90 1135 681 191 1471 Fragments arranged by size: 2451 1471 1135 681 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4342 5243 5505 820 Size: 197 926 2399 901 262 1553 Fragments arranged by size: 2399 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 2478 2978 1517 Size: 961 500 4777 Fragments arranged by size: 4777 961 500 StyI C'CwwG_G Cuts at: 513 1065 2625 3005 3520 3677 513 Size: 552 1560 380 515 157 3074 Fragments arranged by size: 3074 1560 552 515 380 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 4265 4282 4435 4620 5065 325 Size: 1255 762 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 762 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2299 3465 3485 Size: 274 80 33 51 1443 322 1166 20 Cuts at: 3485 4484 6196 96 Size: 999 1712 138 Fragments arranged by size: 1712 1443 1166 999 322 274 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2298 2330 2748 2780 3167 3199 6198 6230 2298 Size: 32 418 32 387 32 2999 32 2306 Fragments arranged by size: 2999 2306 418 387 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI