(Circular) (Six-base) MAPSORT of: pFN21AB8812.seq Check: 2927 from: 1 to: 5797 pFN21AB8812 5797 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 2299 3032 3118 2299 Size: 733 86 4978 Fragments arranged by size: 4978 733 86 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3860 5244 5512 1632 Size: 2228 1384 268 1917 Fragments arranged by size: 2228 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2376 2834 4696 5673 1203 Size: 1173 458 1862 977 1327 Fragments arranged by size: 1862 1327 1173 977 458 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 2101 4526 2101 Size: 2425 3372 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 2779 3540 5112 2779 Size: 761 1572 3464 Fragments arranged by size: 3464 1572 761 ApaI G_GGCC'C Cuts at: 2036 2805 2036 Size: 769 5028 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 2134 3127 3494 2134 Size: 993 367 4437 Fragments arranged by size: 4437 993 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2299 2318 2436 3032 Size: 325 217 627 232 280 19 118 596 Cuts at: 3032 3118 4573 618 Size: 86 1455 1842 Fragments arranged by size: 1842 1455 627 596 325 280 232 217 118 86 19 BanII G_rGCy'C Cuts at: 1391 2036 2455 2514 2805 2814 3116 1391 Size: 645 419 59 291 9 302 4072 Fragments arranged by size: 4072 645 419 302 291 59 9 BbeI G_GCGC'C Cuts at: 2440 2440 Size: 5797 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4826 BbvCI CC'TCA_GC Cuts at: 2625 2625 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2018 2021 2052 3988 4022 1903 Size: 34 50 31 3 31 1936 34 3678 Fragments arranged by size: 3678 1936 50 34 34 31 31 3 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2445 2717 4408 Size: 107 122 71 631 605 773 272 1691 Cuts at: 4408 136 Size: 1525 Fragments arranged by size: 1691 1525 773 631 605 272 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2036 2259 2805 3858 5014 1790 Size: 246 223 546 1053 1156 2573 Fragments arranged by size: 2573 1156 1053 546 246 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2810 2820 3528 Size: 416 53 83 399 24 1393 10 708 Cuts at: 3528 4486 442 Size: 958 1753 Fragments arranged by size: 1753 1393 958 708 416 399 83 53 24 10 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2587 4457 1239 Size: 1348 1870 2579 Fragments arranged by size: 2579 1870 1348 Bpu10I CC'TnA_GC Cuts at: 2625 2625 Size: 5797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 3922 4787 5085 2513 Size: 1409 865 298 3225 Fragments arranged by size: 3225 1409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2935 3006 3056 4460 882 Size: 617 1436 71 50 1404 2219 Fragments arranged by size: 2219 1436 1404 617 71 50 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 2437 3604 3986 Size: 53 83 186 936 569 335 1167 382 Cuts at: 3986 4621 275 Size: 635 1451 Fragments arranged by size: 1451 1167 936 635 569 382 335 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2897 3002 3005 1186 Size: 1711 105 3 3978 Fragments arranged by size: 3978 1711 105 3 BseYI C'CCAG_C Cuts at: 2252 2264 2295 2361 2365 2874 2950 5000 5496 Size: 12 31 66 4 509 76 2050 496 Cuts at: 5496 2252 Size: 2553 Fragments arranged by size: 2553 2050 509 496 76 66 31 12 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 2397 2514 2764 2795 3858 3943 5014 2204 Size: 193 117 250 31 1063 85 1071 2987 Fragments arranged by size: 2987 1071 1063 250 193 117 85 31 BsmI GAATG_Cn' Cuts at: 2040 3308 3401 2040 Size: 1268 93 4436 Fragments arranged by size: 4436 1268 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2109 2109 Size: 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2204 2259 2397 2455 2514 2764 Size: 399 246 168 55 138 58 59 250 Cuts at: 2764 2795 2805 2814 3116 3858 3943 5014 1391 Size: 31 10 9 302 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 250 246 168 138 85 59 58 55 31 10 9 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2239 2496 3152 844 Size: 1395 257 656 3489 Fragments arranged by size: 3489 1395 656 257 BsrBI CCG'CTC Cuts at: 1394 1823 2149 3684 1394 Size: 429 326 1535 3507 Fragments arranged by size: 3507 1535 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2016 4441 4628 1700 Size: 110 206 2425 187 2869 Fragments arranged by size: 2869 2425 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 2010 3857 4869 2010 Size: 1847 1012 2938 Fragments arranged by size: 2938 1847 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 2578 1184 Size: 1394 4403 BstYI r'GATC_y Cuts at: 1972 2134 2427 2524 3127 3494 3879 3896 5348 Size: 162 293 97 603 367 385 17 1452 Cuts at: 5348 5434 5446 5792 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 603 385 367 346 293 162 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2432 3028 2432 Size: 596 5201 BtgI C'CryG_G Cuts at: 513 1065 2081 2187 2336 2715 513 Size: 552 1016 106 149 379 3595 Fragments arranged by size: 3595 1016 552 379 149 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 3313 4107 4127 3313 Size: 794 20 4983 Fragments arranged by size: 4983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DraIII CAC_nnn'GTG Cuts at: 2172 2284 2172 Size: 112 5685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 2718 3244 Size: 54 1425 50 275 18 184 704 526 Cuts at: 3244 4133 5762 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 704 526 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 2519 2875 3727 1353 Size: 839 327 356 852 3423 Fragments arranged by size: 3423 852 839 356 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2512 2512 Size: 5797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2587 3860 4457 5244 5512 1239 Size: 393 955 1273 597 787 268 1524 Fragments arranged by size: 1524 1273 955 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2033 2243 2485 2668 3055 2032 Size: 1 210 242 183 387 4774 Fragments arranged by size: 4774 387 242 210 183 1 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2440 4944 1678 Size: 762 2504 2531 Fragments arranged by size: 2531 2504 762 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2363 2395 4438 Size: 32 135 32 515 32 244 32 2043 Cuts at: 4438 4470 4512 4544 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2043 515 244 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KasI G'GCGC_C Cuts at: 2436 2436 Size: 5797 KpnI G_GTAC'C Cuts at: 2303 3036 3122 2303 Size: 733 86 4978 Fragments arranged by size: 4978 733 86 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 2895 4911 5095 1305 Size: 1461 129 2016 184 2007 Fragments arranged by size: 2016 2007 1461 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 2720 10 Size: 54 1425 343 888 3087 Fragments arranged by size: 3087 1425 888 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2080 3755 4114 4273 518 Size: 648 914 1675 359 159 2042 Fragments arranged by size: 2042 1675 914 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2365 2611 2629 2779 3213 3890 5038 5283 Size: 759 246 18 150 434 677 1148 245 Cuts at: 5283 1606 Size: 2120 Fragments arranged by size: 2120 1148 759 677 434 246 245 150 18 NarI GG'CG_CC Cuts at: 2437 2437 Size: 5797 NcoI C'CATG_G Cuts at: 513 1065 2081 2187 2715 513 Size: 552 1016 106 528 3595 Fragments arranged by size: 3595 1016 552 528 106 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2263 2380 2838 3155 4700 1132 Size: 97 1034 117 458 317 1545 2229 Fragments arranged by size: 2229 1545 1034 458 317 117 97 PasI CC'CwG_GG Cuts at: 1348 2798 1348 Size: 1450 4347 PciI A'CATG_T Cuts at: 2376 2834 4696 2376 Size: 458 1862 3477 Fragments arranged by size: 3477 1862 458 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 2575 1108 Size: 1176 291 4330 Fragments arranged by size: 4330 1176 291 PfoI T'CCnGG_A Cuts at: 2959 2959 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2243 2485 2243 Size: 242 5555 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2032 2801 2032 Size: 769 5028 PstI C_TGCA'G Cuts at: 830 2236 3149 830 Size: 1406 913 3478 Fragments arranged by size: 3478 1406 913 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2365 2611 2629 2779 2365 Size: 246 18 150 5383 Fragments arranged by size: 5383 246 150 18 SacI G_AGCT'C Cuts at: 2514 2514 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SanDI GG'GwC_CC Cuts at: 2243 2243 Size: 5797 SapI GCTCTTCn'nnn_ Cuts at: 2192 2519 2192 Size: 327 5470 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2232 3145 4280 4961 5152 826 Size: 219 1187 913 1135 681 191 1471 Fragments arranged by size: 1471 1187 1135 913 681 219 191 SfoI GGC'GCC Cuts at: 2438 2438 Size: 5797 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 3901 4802 5064 820 Size: 197 926 585 1373 901 262 1553 Fragments arranged by size: 1553 1373 926 901 585 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 2459 1517 Size: 942 4855 StyI C'CwwG_G Cuts at: 513 1065 2081 2187 2715 2784 2805 3236 513 Size: 552 1016 106 528 69 21 431 3074 Fragments arranged by size: 3074 1016 552 528 431 106 69 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2156 2188 5757 5789 2156 Size: 32 3569 32 2164 Fragments arranged by size: 3569 2164 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 2753 3133 2753 Size: 380 5417 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 2538 3926 2538 Size: 1388 4409 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI