Restriction map for
pFN21AB3240
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AcyI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BsaAI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
KasI KspI MfeI MluI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1290 GGTACC G^GTACC
AccII 2267 CGCG CG^CG
AccIII 2039 TCCGGA T^CCGGA
AclI 1004 AACGTT AA^CGTT
AflIII 2586 ACATGT A^C[GA][CT]GT
Alw44I 1961 GTGCAC G^TGCAC
AlwI 55 GATCC GGATC(4/5)
AlwNI 683 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2217 CCTTAGG CC^T[ACGT]AGG
ApaLI 1961 GTGCAC G^TGCAC
Asp718I 1290 GGTACC G^GTACC
AspHI 1961 GTGCAC G[AT]GC[AT]^C
AvaI 1033 CCCGAG C^[CT]CG[GA]G
BanI 1290 GGTACC G^G[CT][GA]CC
BglII 240 AGATCT A^GATCT
BsaBI 880 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2238 GAGACC GGTCTC(1/5)
BsaWI 2039 TCCGGA [AT]^CCGG[AT]
BseAI 2039 TCCGGA T^CCGGA
BsiHKAI 1961 GTGCAC G[AT]GC[AT]^C
BsmAI 2238 GAGAC GTCTC(1/5)
BsoBI 1033 CCCGAG C^[CT]CG[GA]G
BspDI 1834 ATCGAT AT^CGAT
BspEI 2039 TCCGGA T^CCGGA
BspLU11I 2586 ACATGT A^CATGT
BstUI 2267 CGCG CG^CG
BstXI 1164 CCAAATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2217 CCTTAGG CC^T[ACGT]AGG
CfoI 2183 GCGC GCG^C
ClaI 1834 ATCGAT AT^CGAT
DraII 90 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1637 CCACGG C^C[GA][CT]GG
Eco81I 2217 CCTTAGG CC^T[ACGT]AGG
EcoNI 1966 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 90 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 813 GAATTC G^AATTC
EcoRV 72 GATATC GAT^ATC
FauI 2090 GCGGG CCCGC(4/6)
HaeII 2182 AGCGCC [GA]GCGC^[CT]
HapII 2040 CCGG C^CGG
HhaI 2183 GCGC GCG^C
HinP1I 2183 GCGC G^CGC
HindIII 1868 AAGCTT A^AGCTT
HpaI 2410 GTTAAC GTT^AAC
HpaII 2040 CCGG C^CGG
KpnI 1290 GGTACC GGTAC^C
MamI 880 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2039 TCCGGA T^CCGGA
MspA1I 2011 CAGCTG C[AC]G^C[GT]G
MspI 2040 CCGG C^CGG
MvnI 2267 CGCG CG^CG
NdeI 2422 CATATG CA^TATG
PflMI 1648 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 90 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1004 AACGTT AA^CGTT
PvuII 2011 CAGCTG CAG^CTG
SspI 218 AATATT AAT^ATT
StuI 1503 AGGCCT AGG^CCT
Van91I 1648 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 2023 CTTAAG C^TTAAG
2353 CTTAAG
Asp700I 148 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1059 GAAACAATTC
BalI 2050 TGGCCA TGG^CCA
2078 TGGCCA
BbsI 2261 GAAGAC GAAGAC(2/6)
2211 GTCTTC
BfaI 2099 CTAG C^TAG
2279 CTAG
BfrI 2023 CTTAAG C^TTAAG
2353 CTTAAG
BlpI 805 GCTCAGC GC^T[ACGT]AGC
1300 GCTCAGC
BmyI 1805 GGGCAC G[AGT]GC[ACT]^C
1961 GTGCAC
Bpu1102I 805 GCTCAGC GC^T[ACGT]AGC
1300 GCTCAGC
BpuAI 2261 GAAGAC GAAGAC(2/6)
2211 GTCTTC
BsgI 1661 CTGCAC GTGCAG(16/14)
2014 CTGCAC
Bsp1286I 1805 GGGCAC G[AGT]GC[ACT]^C
1961 GTGCAC
BspHI 156 TCATGA T^CATGA
548 TCATGA
BstYI 54 AGATCC [GA]^GATC[CT]
240 AGATCT
CelII 805 GCTCAGC GC^T[ACGT]AGC
1300 GCTCAGC
EaeI 2050 TGGCCA [CT]^GGCC[GA]
2078 TGGCCA
HincII 396 GTTGAC GT[CT]^[GA]AC
2410 GTTAAC
HindII 396 GTTGAC GT[CT]^[GA]AC
2410 GTTAAC
MaeI 2099 CTAG C^TAG
2279 CTAG
MflI 54 AGATCC [GA]^GATC[CT]
240 AGATCT
MluNI 2050 TGGCCA TGG^CCA
2078 TGGCCA
MscI 2050 TGGCCA TGG^CCA
2078 TGGCCA
PleI 1029 GACTC GAGTC(4/5)
1621 GACTC
RcaI 156 TCATGA T^CATGA
548 TCATGA
ScaI 703 AGTACT AGT^ACT
733 AGTACT
SfaNI 343 GATGC GCATC(5/9)
1837 GATGC
TaqI 314 TCGA T^CGA
1835 TCGA
TthHB8I 314 TCGA T^CGA
1835 TCGA
XhoII 54 AGATCC [GA]^GATC[CT]
240 AGATCT
XmnI 148 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1059 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BpmI BsaJI BseRI
BsiYI BslI BsmFI BsmI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PstI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI