(Circular) (Six-base) MAPSORT of: pFN21AB3240.seq Check: 442 from: 1 to: 7324 pFN21AB3240 7324 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5134 278 Size: 53 83 186 936 3598 2468 Fragments arranged by size: 3598 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4667 1937 Size: 2730 4594 Acc65I G'GTAC_C Cuts at: 3295 4645 3295 Size: 1350 5974 AclI AA'CG_TT Cuts at: 1563 3010 5451 5824 1563 Size: 1447 2441 373 3063 Fragments arranged by size: 3063 2441 1447 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2646 2670 3737 4394 5387 6771 7039 1632 Size: 1014 24 1067 657 993 1384 268 1917 Fragments arranged by size: 1917 1384 1067 1014 993 657 268 24 AflII C'TTAA_G Cuts at: 820 1017 4028 4358 820 Size: 197 3011 330 3786 Fragments arranged by size: 3786 3011 330 197 AflIII A'CryG_T Cuts at: 1203 4591 6223 7200 1203 Size: 3388 1632 977 1327 Fragments arranged by size: 3388 1632 1327 977 AgeI A'CCGG_T Cuts at: 6155 6155 Size: 7324 AhdI GACnn_n'nnGTC Cuts at: 6053 6053 Size: 7324 AleI CACnn'nnGTG Cuts at: 1166 2753 1166 Size: 1587 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7324 AlwNI CAG_nnn'CTG Cuts at: 2693 5067 6639 2693 Size: 2374 1572 3378 Fragments arranged by size: 3378 2374 1572 ApaLI G'TGCA_C Cuts at: 3966 5381 6537 3966 Size: 1415 1156 4753 Fragments arranged by size: 4753 1415 1156 ApoI r'AATT_y Cuts at: 1493 2299 2392 2818 3565 4334 4633 4859 5011 Size: 806 93 426 747 769 299 226 152 Cuts at: 5011 7120 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 806 769 747 426 299 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 5878 160 Size: 872 4846 1606 Fragments arranged by size: 4846 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7324 AvaI C'yCGr_G Cuts at: 1870 1943 3038 4649 1870 Size: 73 1095 1611 4545 Fragments arranged by size: 4545 1611 1095 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2787 2820 2787 Size: 33 7291 BamHI G'GATC_C Cuts at: 4654 5021 4654 Size: 367 6957 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4645 6100 618 Size: 325 217 627 1508 1350 1455 1842 Fragments arranged by size: 1842 1508 1455 1350 627 325 217 BanII G_rGCy'C Cuts at: 1391 4643 1391 Size: 3252 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4209 4273 928 Size: 971 2310 64 3979 Fragments arranged by size: 3979 2310 971 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5515 5549 1903 Size: 34 3578 34 3678 Fragments arranged by size: 3678 3578 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5216 6426 5216 Size: 1210 6114 BclI T'GATC_A Cuts at: 1548 1548 Size: 7324 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5935 136 Size: 107 122 71 631 605 4263 1525 Fragments arranged by size: 4263 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2245 7319 2245 Size: 5074 2250 BlpI GC'TnA_GC Cuts at: 2811 3306 4741 2811 Size: 495 1435 5394 Fragments arranged by size: 5394 1435 495 Bme1580I G_kGCm'C Cuts at: 1790 3814 3970 5385 6541 1790 Size: 2024 156 1415 1156 2573 Fragments arranged by size: 2573 2024 1415 1156 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4318 5055 6013 Size: 416 53 83 399 24 2901 737 958 Cuts at: 6013 442 Size: 1753 Fragments arranged by size: 2901 1753 958 737 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7324 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 7324 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2226 3613 3957 5984 1239 Size: 987 1387 344 2027 2579 Fragments arranged by size: 2579 2027 1387 987 344 Bpu10I CC'TnA_GC Cuts at: 3675 4011 3675 Size: 336 6988 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 2128 2946 3186 5449 6314 6612 2090 Size: 38 818 240 2263 865 298 2802 Fragments arranged by size: 2802 2263 865 818 298 240 38 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4237 5987 882 Size: 617 2738 1750 2219 Fragments arranged by size: 2738 2219 1750 617 BsaAI yAC'GTr Cuts at: 493 1204 5115 493 Size: 711 3911 2702 Fragments arranged by size: 3911 2702 711 BsaBI GATnn'nnATC Cuts at: 2889 7318 2889 Size: 4429 2895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5131 5513 6148 275 Size: 53 83 186 936 3598 382 635 1451 Fragments arranged by size: 3598 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 5757 6155 6429 6576 6987 4044 Size: 1713 398 274 147 411 4381 Fragments arranged by size: 4381 1713 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3127 3157 1178 Size: 30 165 30 1724 30 5345 Fragments arranged by size: 5345 1724 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 3065 4526 1186 Size: 1312 567 1461 3984 Fragments arranged by size: 3984 1461 1312 567 BseYI C'CCAG_C Cuts at: 6527 7023 6527 Size: 496 6828 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3651 4004 1163 Size: 573 1915 353 4483 Fragments arranged by size: 4483 1915 573 353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4774 5535 6563 1540 Size: 275 186 2773 761 1028 2301 Fragments arranged by size: 2773 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3970 5385 5470 6541 3970 Size: 1415 85 1071 4753 Fragments arranged by size: 4753 1415 1071 85 BsmI GAATG_Cn' Cuts at: 2327 2494 2859 3402 4835 4928 2327 Size: 167 365 543 1433 93 4723 Fragments arranged by size: 4723 1433 543 365 167 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3814 3970 4643 5385 5470 6541 1391 Size: 399 2024 156 673 742 85 1071 2174 Fragments arranged by size: 2174 2024 1071 742 673 399 156 85 BspEI T'CCGG_A Cuts at: 4044 6987 4044 Size: 2943 4381 BspHI T'CATG_A Cuts at: 2161 2553 5213 6943 2161 Size: 392 2660 1730 2542 Fragments arranged by size: 2660 2542 1730 392 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3307 3905 4432 4679 844 Size: 2463 598 527 247 3489 Fragments arranged by size: 3489 2463 598 527 247 BsrBI CCG'CTC Cuts at: 1394 1823 5211 1394 Size: 429 3388 3507 Fragments arranged by size: 3507 3388 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5819 5993 65 Size: 1183 4571 174 1396 Fragments arranged by size: 4571 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5968 6155 1700 Size: 110 4158 187 2869 Fragments arranged by size: 4158 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7324 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7324 BssSI C'ACGA_G Cuts at: 5384 6396 5384 Size: 1012 6312 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7324 BstBI TT'CG_AA Cuts at: 6128 6128 Size: 7324 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7324 BstXI CCAn_nnnn'nTGG Cuts at: 3176 3176 Size: 7324 BstYI r'GATC_y Cuts at: 1972 2059 2245 4654 5021 5406 5423 6875 6961 Size: 87 186 2409 367 385 17 1452 86 Cuts at: 6961 6973 7319 1972 Size: 12 346 1977 Fragments arranged by size: 2409 1977 1452 385 367 346 186 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4223 4223 Size: 7324 BtgI C'CryG_G Cuts at: 513 1065 2004 3642 513 Size: 552 939 1638 4195 Fragments arranged by size: 4195 1638 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6585 Fragments arranged by size: 6585 683 56 BtsI GCAGTG_nn' Cuts at: 4840 5634 5654 4840 Size: 794 20 6510 Fragments arranged by size: 6510 794 20 ClaI AT'CG_AT Cuts at: 1554 3840 1554 Size: 2286 5038 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7289 DraI TTT'AAA Cuts at: 3015 3721 3879 4339 4628 3015 Size: 706 158 460 289 5711 Fragments arranged by size: 5711 706 460 289 158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6331 809 Size: 507 5015 1802 Fragments arranged by size: 5015 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4055 4083 4771 Size: 54 1425 50 275 18 2225 28 688 Cuts at: 4771 5660 7289 8 Size: 889 1629 43 Fragments arranged by size: 2225 1629 1425 889 688 275 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 4771 1537 Size: 275 2959 4090 Fragments arranged by size: 4090 2959 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2382 2622 3826 3879 4062 4255 4536 Size: 795 234 240 1204 53 183 193 281 Cuts at: 4536 5254 1353 Size: 718 3423 Fragments arranged by size: 3423 1204 795 718 281 240 234 193 183 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5911 6285 6431 387 Size: 1064 138 4322 374 146 1280 Fragments arranged by size: 4322 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2226 2646 2670 3613 3737 3957 4394 Size: 393 594 420 24 943 124 220 437 Cuts at: 4394 5387 5984 6771 7039 1239 Size: 993 597 787 268 1524 Fragments arranged by size: 1524 993 943 787 597 594 437 420 393 268 220 124 24 EcoNI CCTnn'n_nnAGG Cuts at: 3975 3975 Size: 7324 EcoO109I rG'GnC_Cy Cuts at: 2096 2096 Size: 7324 EcoRI G'AATT_C Cuts at: 2818 4633 5011 2818 Size: 1815 378 5131 Fragments arranged by size: 5131 1815 378 EcoRV GAT'ATC Cuts at: 2079 2079 Size: 7324 FspI TGC'GCA Cuts at: 5830 5830 Size: 7324 HaeII r_GCGC'y Cuts at: 1678 4191 6471 1678 Size: 2513 2280 2531 Fragments arranged by size: 2531 2513 2280 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 4311 4343 5965 Size: 32 135 32 491 32 2216 32 1622 Cuts at: 5965 5997 6039 6071 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2216 1622 491 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2403 4417 4668 4914 669 Size: 988 281 465 2014 251 246 3079 Fragments arranged by size: 3079 2014 988 465 281 251 246 HindIII A'AGCT_T Cuts at: 748 3873 748 Size: 3125 4199 HpaI GTT'AAC Cuts at: 4417 4914 4417 Size: 497 6827 KpnI G_GTAC'C Cuts at: 3299 4649 3299 Size: 1350 5974 MfeI C'AATT_G Cuts at: 4923 4923 Size: 7324 MluI A'CGCG_T Cuts at: 7200 7200 Size: 7324 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3011 3371 6438 6622 1305 Size: 1706 360 3067 184 2007 Fragments arranged by size: 3067 2007 1706 360 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4057 4085 10 Size: 54 1425 343 2225 28 3249 Fragments arranged by size: 3249 2225 1425 343 54 28 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2558 2753 5282 5641 5800 518 Size: 648 1120 272 195 2529 359 159 2042 Fragments arranged by size: 2529 2042 1120 648 359 272 195 159 MspA1I CmG'CkG Cuts at: 1606 4018 4740 5417 6565 6810 1606 Size: 2412 722 677 1148 245 2120 Fragments arranged by size: 2412 2120 1148 722 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5833 Fragments arranged by size: 5833 939 552 NdeI CA'TA_TG Cuts at: 387 4428 387 Size: 4041 3283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7324 NotI GC'GGCC_GC Cuts at: 4771 4771 Size: 7324 NspI r_CATG'y Cuts at: 1132 1229 3168 4027 4595 4682 6227 1132 Size: 97 1939 859 568 87 1545 2229 Fragments arranged by size: 2229 1939 1545 859 568 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7324 PciI A'CATG_T Cuts at: 4591 6223 4591 Size: 1632 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 3659 1108 Size: 2551 4773 PfoI T'CCnGG_A Cuts at: 2776 3683 2776 Size: 907 6417 PmeI GTTT'AAAC Cuts at: 4628 4628 Size: 7324 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5393 5425 6906 6938 5393 Size: 32 1481 32 5779 Fragments arranged by size: 5779 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2096 Size: 7324 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7324 PsiI TTA'TAA Cuts at: 4894 4894 Size: 7324 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3070 3102 3070 Size: 32 7292 PstI C_TGCA'G Cuts at: 830 3319 3736 3955 4676 830 Size: 2489 417 219 721 3478 Fragments arranged by size: 3478 2489 721 417 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7324 PvuII CAG'CTG Cuts at: 4018 4018 Size: 7324 SalI G'TCGA_C Cuts at: 1936 4666 1936 Size: 2730 4594 SbfI CC_TGCA'GG Cuts at: 4676 4676 Size: 7324 ScaI AGT'ACT Cuts at: 2710 2740 5572 7284 2710 Size: 30 2832 1712 2750 Fragments arranged by size: 2832 2750 1712 30 SfcI C'TryA_G Cuts at: 826 1045 2115 2508 3240 3315 3732 3951 4672 Size: 219 1070 393 732 75 417 219 721 Cuts at: 4672 5807 6488 6679 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1070 732 721 681 417 393 219 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7324 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7324 SmaI CCC'GGG Cuts at: 1872 4651 1872 Size: 2779 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2143 2961 3201 4028 4358 Size: 197 926 126 74 818 240 827 330 Cuts at: 4358 5428 6329 6591 820 Size: 1070 901 262 1553 Fragments arranged by size: 1553 1070 926 901 827 818 330 262 240 197 126 74 SnaBI TAC'GTA Cuts at: 493 5115 493 Size: 4622 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7324 SphI G_CATG'C Cuts at: 1132 4682 1132 Size: 3550 3774 SspI AAT'ATT Cuts at: 5 52 2225 5248 5 Size: 47 2173 3023 2081 Fragments arranged by size: 3023 2173 2081 47 StuI AGG'CCT Cuts at: 1517 3510 1517 Size: 1993 5331 StyI C'CwwG_G Cuts at: 513 1065 2004 4763 513 Size: 552 939 2759 3074 Fragments arranged by size: 3074 2759 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2394 5351 5368 5521 5706 6151 325 Size: 1255 814 2957 17 153 185 445 1498 Fragments arranged by size: 2957 1498 1255 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2228 2708 2738 Size: 274 80 33 51 1443 251 480 30 Cuts at: 2738 3778 3983 5570 7282 96 Size: 1040 205 1587 1712 138 Fragments arranged by size: 1712 1587 1443 1040 480 274 251 205 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7284 7316 7284 Size: 32 7292 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7324 XbaI T'CTAG_A Cuts at: 4660 4660 Size: 7324 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7324 XmaI C'CCGG_G Cuts at: 1870 4649 1870 Size: 2779 4545 XmnI GAAnn'nnTTC Cuts at: 2157 3068 5453 2157 Size: 911 2385 4028 Fragments arranged by size: 4028 2385 911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5132 276 Size: 53 83 186 936 3598 2468 Fragments arranged by size: 3598 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI