Restriction map for
pFN21AB3240
(Linear) (Six-base) MAPSORT of: KIBB3240.seq Check: 5449 from: 1 to: 2619
KIBB3240.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 1290 2619
Size: 1290 1329
AclI AA'CG_TT
Cuts at: 0 1005 2619
Size: 1005 1614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 665 1732 2389 2619
Size: 641 24 1067 657 230
Fragments arranged by size:
1067 657 641 230 24
AflII C'TTAA_G
Cuts at: 0 2023 2353 2619
Size: 2023 330 266
Fragments arranged by size:
2023 330 266
AflIII A'CryG_T
Cuts at: 0 2586 2619
Size: 2586 33
AleI CACnn'nnGTG
Cuts at: 0 748 2619
Size: 748 1871
AlwNI CAG_nnn'CTG
Cuts at: 0 688 2619
Size: 688 1931
ApaLI G'TGCA_C
Cuts at: 0 1961 2619
Size: 1961 658
ApoI r'AATT_y
Cuts at: 0 294 387 813 1560 2329 2619
Size: 294 93 426 747 769 290
Fragments arranged by size:
769 747 426 294 290 93
AvaI C'yCGr_G
Cuts at: 0 1033 2619
Size: 1033 1586
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 782 815 2619
Size: 782 33 1804
Fragments arranged by size:
1804 782 33
BanI G'GyrC_C
Cuts at: 0 1290 2619
Size: 1290 1329
BbsI GAAGACnn'nnnn_
Cuts at: 0 2204 2268 2619
Size: 2204 64 351
Fragments arranged by size:
2204 351 64
BglII A'GATC_T
Cuts at: 0 240 2619
Size: 240 2379
BlpI GC'TnA_GC
Cuts at: 0 806 1301 2619
Size: 806 495 1318
Fragments arranged by size:
1318 806 495
Bme1580I G_kGCm'C
Cuts at: 0 1809 1965 2619
Size: 1809 156 654
Fragments arranged by size:
1809 654 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 2313 2619
Size: 2313 306
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 2619
Size: 153 2466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 1608 1952 2619
Size: 221 1387 344 667
Fragments arranged by size:
1387 667 344 221
Bpu10I CC'TnA_GC
Cuts at: 0 1670 2006 2619
Size: 1670 336 613
Fragments arranged by size:
1670 613 336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 123 941 1181 2619
Size: 85 38 818 240 1438
Fragments arranged by size:
1438 818 240 85 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 2232 2619
Size: 2232 387
BsaBI GATnn'nnATC
Cuts at: 0 884 2619
Size: 884 1735
BsaWI w'CCGG_w
Cuts at: 0 2039 2619
Size: 2039 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1122 1152 2619
Size: 1122 30 1467
Fragments arranged by size:
1467 1122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1060 2521 2619
Size: 493 567 1461 98
Fragments arranged by size:
1461 567 493 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1646 1999 2619
Size: 1646 353 620
Fragments arranged by size:
1646 620 353
BsiHKAI G_wGCw'C
Cuts at: 0 1965 2619
Size: 1965 654
BsmI GAATG_Cn'
Cuts at: 0 322 489 854 1397 2619
Size: 322 167 365 543 1222
Fragments arranged by size:
1222 543 365 322 167
Bsp1286I G_dGCh'C
Cuts at: 0 1809 1965 2619
Size: 1809 156 654
Fragments arranged by size:
1809 654 156
BspEI T'CCGG_A
Cuts at: 0 2039 2619
Size: 2039 580
BspHI T'CATG_A
Cuts at: 0 156 548 2619
Size: 156 392 2071
Fragments arranged by size:
2071 392 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1302 1900 2427 2619
Size: 1302 598 527 192
Fragments arranged by size:
1302 598 527 192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1171 2619
Size: 1171 1448
BstYI r'GATC_y
Cuts at: 0 54 240 2619
Size: 54 186 2379
Fragments arranged by size:
2379 186 54
Bsu36I CC'TnA_GG
Cuts at: 0 2218 2619
Size: 2218 401
BtgI C'CryG_G
Cuts at: 0 1637 2619
Size: 1637 982
ClaI AT'CG_AT
Cuts at: 0 1835 2619
Size: 1835 784
DraI TTT'AAA
Cuts at: 0 1010 1716 1874 2334 2619
Size: 1010 706 158 460 285
Fragments arranged by size:
1010 706 460 285 158
EaeI y'GGCC_r
Cuts at: 0 2050 2078 2619
Size: 2050 28 541
Fragments arranged by size:
2050 541 28
EarI CTCTTCn'nnn_
Cuts at: 0 143 377 617 1821 1874 2057 2250 2531
Size: 143 234 240 1204 53 183 193 281
Cuts at: 2531 2619
Size: 88
Fragments arranged by size:
1204 281 240 234 193 183 143 88
53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 641 665 1608 1732 1952 2389 2619
Size: 221 420 24 943 124 220 437 230
Fragments arranged by size:
943 437 420 230 221 220 124 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1970 2619
Size: 1970 649
EcoO109I rG'GnC_Cy
Cuts at: 0 91 2619
Size: 91 2528
EcoRI G'AATT_C
Cuts at: 0 813 2619
Size: 813 1806
EcoRV GAT'ATC
Cuts at: 0 74 2619
Size: 74 2545
HaeII r_GCGC'y
Cuts at: 0 2186 2619
Size: 2186 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 2306 2338 2619
Size: 58 32 2216 32 281
Fragments arranged by size:
2216 281 58 32 32
HincII GTy'rAC
Cuts at: 0 398 2412 2619
Size: 398 2014 207
Fragments arranged by size:
2014 398 207
HindIII A'AGCT_T
Cuts at: 0 1868 2619
Size: 1868 751
HpaI GTT'AAC
Cuts at: 0 2412 2619
Size: 2412 207
KpnI G_GTAC'C
Cuts at: 0 1294 2619
Size: 1294 1325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1366 2619
Size: 1006 360 1253
Fragments arranged by size:
1253 1006 360
MscI TGG'CCA
Cuts at: 0 2052 2080 2619
Size: 2052 28 539
Fragments arranged by size:
2052 539 28
MslI CAynn'nnrTG
Cuts at: 0 281 553 748 2619
Size: 281 272 195 1871
Fragments arranged by size:
1871 281 272 195
MspA1I CmG'CkG
Cuts at: 0 2013 2619
Size: 2013 606
NdeI CA'TA_TG
Cuts at: 0 2423 2619
Size: 2423 196
NspI r_CATG'y
Cuts at: 0 1163 2022 2590 2619
Size: 1163 859 568 29
Fragments arranged by size:
1163 859 568 29
PciI A'CATG_T
Cuts at: 0 2586 2619
Size: 2586 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 1654 2619
Size: 1654 965
PfoI T'CCnGG_A
Cuts at: 0 771 1678 2619
Size: 771 907 941
Fragments arranged by size:
941 907 771
PpuMI rG'GwC_Cy
Cuts at: 0 91 2619
Size: 91 2528
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1065 1097 2619
Size: 1065 32 1522
Fragments arranged by size:
1522 1065 32
PstI C_TGCA'G
Cuts at: 0 1314 1731 1950 2619
Size: 1314 417 219 669
Fragments arranged by size:
1314 669 417 219
PvuII CAG'CTG
Cuts at: 0 2013 2619
Size: 2013 606
ScaI AGT'ACT
Cuts at: 0 705 735 2619
Size: 705 30 1884
Fragments arranged by size:
1884 705 30
SfcI C'TryA_G
Cuts at: 0 110 503 1235 1310 1727 1946 2619
Size: 110 393 732 75 417 219 673
Fragments arranged by size:
732 673 417 393 219 110 75
SmlI C'TyrA_G
Cuts at: 0 64 138 956 1196 2023 2353 2619
Size: 64 74 818 240 827 330 266
Fragments arranged by size:
827 818 330 266 240 74 64
SspI AAT'ATT
Cuts at: 0 220 2619
Size: 220 2399
StuI AGG'CCT
Cuts at: 0 1505 2619
Size: 1505 1114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 389 2619
Size: 389 2230
TatI w'GTAC_w
Cuts at: 0 223 703 733 1773 1978 2619
Size: 223 480 30 1040 205 641
Fragments arranged by size:
1040 641 480 223 205 30
XmnI GAAnn'nnTTC
Cuts at: 0 152 1063 2619
Size: 152 911 1556
Fragments arranged by size:
1556 911 152
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AleI AlwNI ApaLI
ApoI AvaI BaeI BanI BbsI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BstXI BstYI Bsu36I BtgI ClaI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NdeI NspI PciI PflMI PfoI PpuMI PsrI PstI
PvuII ScaI SfcI SmlI SspI StuI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvrII BamHI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI
BsaAI BsaHI BseYI BsiEI BsiWI BsmBI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI CspCI DraIII DrdI EagI EciI EcoICRI
FalI FseI FspI FspAI KasI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StyI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI