(Circular) (Six-base) MAPSORT of: pFN21AB3091.seq Check: 6127 from: 1 to: 7507 pFN21AB3091 7507 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5317 278 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 3969 4850 1937 Size: 1755 277 881 4594 Fragments arranged by size: 4594 1755 881 277 Acc65I G'GTAC_C Cuts at: 4828 4828 Size: 7507 AclI AA'CG_TT Cuts at: 1563 5634 6007 1563 Size: 4071 373 3063 Fragments arranged by size: 4071 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2875 3744 4446 4764 5570 6954 7222 1632 Size: 1243 869 702 318 806 1384 268 1917 Fragments arranged by size: 1917 1384 1243 869 806 702 318 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7310 AflIII A'CryG_T Cuts at: 1203 3834 4405 6406 7383 1203 Size: 2631 571 2001 977 1327 Fragments arranged by size: 2631 2001 1327 977 571 AgeI A'CCGG_T Cuts at: 2730 6338 2730 Size: 3608 3899 AhdI GACnn_n'nnGTC Cuts at: 6236 6236 Size: 7507 AleI CACnn'nnGTG Cuts at: 1166 2102 1166 Size: 936 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4636 1184 Size: 3452 4055 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3837 3869 3837 Size: 32 7475 AlwNI CAG_nnn'CTG Cuts at: 2980 3265 5250 6822 2980 Size: 285 1985 1572 3665 Fragments arranged by size: 3665 1985 1572 285 ApaLI G'TGCA_C Cuts at: 3552 5564 6720 3552 Size: 2012 1156 4339 Fragments arranged by size: 4339 2012 1156 ApoI r'AATT_y Cuts at: 1493 2254 2534 2667 3085 3404 3653 4816 5042 Size: 761 280 133 418 319 249 1163 226 Cuts at: 5042 5194 7303 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1163 761 418 319 280 249 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 3491 4235 6061 160 Size: 872 2459 744 1826 1606 Fragments arranged by size: 2459 1826 1606 872 744 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 AvaI C'yCGr_G Cuts at: 1870 1943 2166 4134 4832 1870 Size: 73 223 1968 698 4545 Fragments arranged by size: 4545 1968 698 223 73 BamHI G'GATC_C Cuts at: 4837 5204 4837 Size: 367 7140 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4828 6283 618 Size: 325 217 627 3041 1455 1842 Fragments arranged by size: 3041 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2932 4826 1391 Size: 1541 1894 4072 Fragments arranged by size: 4072 1894 1541 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2406 928 Size: 971 507 6029 Fragments arranged by size: 6029 971 507 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5698 5732 1903 Size: 34 3761 34 3678 Fragments arranged by size: 3761 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5399 6609 5399 Size: 1210 6297 BclI T'GATC_A Cuts at: 1548 2193 2548 2673 4471 1548 Size: 645 355 125 1798 4584 Fragments arranged by size: 4584 1798 645 355 125 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6118 136 Size: 107 122 71 631 605 4446 1525 Fragments arranged by size: 4446 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2275 3160 3508 7502 2275 Size: 885 348 3994 2280 Fragments arranged by size: 3994 2280 885 348 BlpI GC'TnA_GC Cuts at: 3804 4924 3804 Size: 1120 6387 Bme1580I G_kGCm'C Cuts at: 1790 3556 5568 6724 1790 Size: 1766 2012 1156 2573 Fragments arranged by size: 2573 2012 1766 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7507 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4059 5238 6196 Size: 416 53 83 399 24 2642 1179 958 Cuts at: 6196 442 Size: 1753 Fragments arranged by size: 2642 1753 1179 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7507 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2646 6167 1239 Size: 1407 3521 2579 Fragments arranged by size: 3521 2579 1407 Bpu10I CC'TnA_GC Cuts at: 4515 4515 Size: 7507 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2585 2997 4577 5632 6497 6795 2585 Size: 412 1580 1055 865 298 3297 Fragments arranged by size: 3297 1580 1055 865 412 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 6170 882 Size: 617 2925 1746 2219 Fragments arranged by size: 2925 2219 1746 617 BsaAI yAC'GTr Cuts at: 493 1204 3835 5298 493 Size: 711 2631 1463 2702 Fragments arranged by size: 2702 2631 1463 711 BsaBI GATnn'nnATC Cuts at: 2547 3384 3432 7501 2547 Size: 837 48 4069 2553 Fragments arranged by size: 4069 2553 837 48 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5314 5696 6331 275 Size: 53 83 186 936 3781 382 635 1451 Fragments arranged by size: 3781 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2730 5940 6338 6612 6759 7170 2730 Size: 3210 398 274 147 411 3067 Fragments arranged by size: 3210 3067 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3837 3867 4553 4583 4652 Size: 30 165 30 2434 30 686 30 69 Cuts at: 4652 4682 1178 Size: 30 4003 Fragments arranged by size: 4003 2434 686 165 69 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 2628 2676 3089 3309 4574 1186 Size: 1378 64 48 413 220 1265 4119 Fragments arranged by size: 4119 1378 1265 413 220 64 48 BseYI C'CCAG_C Cuts at: 6710 7206 6710 Size: 496 7011 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 3519 1163 Size: 573 297 1486 5151 Fragments arranged by size: 5151 1486 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2119 4957 5718 6746 1540 Size: 275 186 48 70 2838 761 1028 2301 Fragments arranged by size: 2838 2301 1028 761 275 186 70 48 BsiHKAI G_wGCw'C Cuts at: 3556 3850 5568 5653 6724 3556 Size: 294 1718 85 1071 4339 Fragments arranged by size: 4339 1718 1071 294 85 BsmI GAATG_Cn' Cuts at: 5018 5111 5018 Size: 93 7414 BsmBI CGTCTCn'nnnn_ Cuts at: 2018 2018 Size: 7507 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2932 3556 3850 4826 5568 5653 6724 Size: 399 1142 624 294 976 742 85 1071 Cuts at: 6724 1391 Size: 2174 Fragments arranged by size: 2174 1142 1071 976 742 624 399 294 85 BspEI T'CCGG_A Cuts at: 7170 7170 Size: 7507 BspHI T'CATG_A Cuts at: 4357 5396 7126 4357 Size: 1039 1730 4738 Fragments arranged by size: 4738 1730 1039 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4862 844 Size: 4018 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5394 1394 Size: 429 3571 3507 Fragments arranged by size: 3571 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4311 6002 6176 65 Size: 1183 3063 1691 174 1396 Fragments arranged by size: 3063 1691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2209 2730 6151 6338 1700 Size: 110 399 521 3421 187 2869 Fragments arranged by size: 3421 2869 521 399 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7507 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7507 BssSI C'ACGA_G Cuts at: 5567 6579 5567 Size: 1012 6495 BstAPI GCAn_nnn'nTGC Cuts at: 794 4502 794 Size: 3708 3799 BstBI TT'CG_AA Cuts at: 6311 6311 Size: 7507 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7507 BstXI CCAn_nnnn'nTGG Cuts at: 3644 4180 3644 Size: 536 6971 BstYI r'GATC_y Cuts at: 1972 2275 3160 3508 4837 5204 5589 5606 7058 Size: 303 885 348 1329 367 385 17 1452 Cuts at: 7058 7144 7156 7502 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1329 885 385 367 348 346 303 86 17 12 BstZ17I GTA'TAC Cuts at: 3693 3970 3693 Size: 277 7230 Bsu36I CC'TnA_GG Cuts at: 4211 4211 Size: 7507 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6016 Fragments arranged by size: 6016 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6768 Fragments arranged by size: 6768 683 56 BtsI GCAGTG_nn' Cuts at: 5023 5817 5837 5023 Size: 794 20 6693 Fragments arranged by size: 6693 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7472 DraI TTT'AAA Cuts at: 3681 4811 3681 Size: 1130 6377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6514 809 Size: 507 5198 1802 Fragments arranged by size: 5198 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2160 2473 Size: 54 1425 50 275 18 216 114 313 Cuts at: 2473 4374 4954 5843 7472 8 Size: 1901 580 889 1629 43 Fragments arranged by size: 1901 1629 1425 889 580 313 275 216 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 4954 1537 Size: 275 234 2908 4090 Fragments arranged by size: 4090 2908 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 3681 4252 4422 5437 1353 Size: 2328 571 170 1015 3423 Fragments arranged by size: 3423 2328 1015 571 170 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6094 6468 6614 387 Size: 1064 138 4505 374 146 1280 Fragments arranged by size: 4505 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2646 2875 3744 4446 4764 5570 6167 Size: 393 1014 229 869 702 318 806 597 Cuts at: 6167 6954 7222 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1014 869 806 787 702 597 393 318 268 229 EcoNI CCTnn'n_nnAGG Cuts at: 2942 4670 2942 Size: 1728 5779 EcoO109I rG'GnC_Cy Cuts at: 2722 2722 Size: 7507 EcoRI G'AATT_C Cuts at: 2534 2667 3404 4816 5194 2534 Size: 133 737 1412 378 4847 Fragments arranged by size: 4847 1412 737 378 133 EcoRV GAT'ATC Cuts at: 3634 4597 3634 Size: 963 6544 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2297 3161 4281 2297 Size: 864 1120 5523 Fragments arranged by size: 5523 1120 864 FspI TGC'GCA Cuts at: 6013 6013 Size: 7507 HaeII r_GCGC'y Cuts at: 1678 6654 1678 Size: 4976 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 4581 4613 4652 Size: 32 135 32 968 32 2009 32 39 Cuts at: 4652 4684 6148 6180 6222 6254 1373 Size: 32 1464 32 42 32 2626 Fragments arranged by size: 2626 2009 1464 968 135 42 39 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3031 3486 4851 5097 669 Size: 988 281 1093 455 1365 246 3079 Fragments arranged by size: 3079 1365 1093 988 455 281 246 HindIII A'AGCT_T Cuts at: 748 3096 748 Size: 2348 5159 HpaI GTT'AAC Cuts at: 3031 5097 3031 Size: 2066 5441 KpnI G_GTAC'C Cuts at: 4832 4832 Size: 7507 MfeI C'AATT_G Cuts at: 4157 5106 4157 Size: 949 6558 MluI A'CGCG_T Cuts at: 7383 7383 Size: 7507 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 2467 2880 4173 6621 6805 1305 Size: 711 451 413 1293 2448 184 2007 Fragments arranged by size: 2448 2007 1293 711 451 413 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2475 4376 10 Size: 54 1425 343 643 1901 3141 Fragments arranged by size: 3141 1901 1425 643 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2102 3924 4178 4356 5465 5824 Size: 648 843 93 1822 254 178 1109 359 Cuts at: 5824 5983 518 Size: 159 2042 Fragments arranged by size: 2042 1822 1109 843 648 359 254 178 159 93 MspA1I CmG'CkG Cuts at: 1606 4923 5600 6748 6993 1606 Size: 3317 677 1148 245 2120 Fragments arranged by size: 3317 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2211 2211 Size: 7507 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6016 Fragments arranged by size: 6016 939 552 NdeI CA'TA_TG Cuts at: 387 3859 387 Size: 3472 4035 NgoMIV G'CCGG_C Cuts at: 2209 2209 Size: 7507 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7507 NotI GC'GGCC_GC Cuts at: 4954 4954 Size: 7507 NspI r_CATG'y Cuts at: 1132 1229 2083 3923 4342 4409 4523 4865 6410 Size: 97 854 1840 419 67 114 342 1545 Cuts at: 6410 1132 Size: 2229 Fragments arranged by size: 2229 1840 1545 854 419 342 114 97 67 PasI CC'CwG_GG Cuts at: 1348 2185 1348 Size: 837 6670 PciI A'CATG_T Cuts at: 4405 6406 4405 Size: 2001 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7507 PmeI GTTT'AAAC Cuts at: 4811 4811 Size: 7507 PmlI CAC'GTG Cuts at: 3835 3835 Size: 7507 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3837 3869 5576 5608 7089 7121 3837 Size: 32 1707 32 1481 32 4223 Fragments arranged by size: 4223 1707 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2722 2722 Size: 7507 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7507 PsiI TTA'TAA Cuts at: 2313 5077 2313 Size: 2764 4743 PstI C_TGCA'G Cuts at: 830 2034 2232 4859 830 Size: 1204 198 2627 3478 Fragments arranged by size: 3478 2627 1204 198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7507 SalI G'TCGA_C Cuts at: 1936 4849 1936 Size: 2913 4594 SbfI CC_TGCA'GG Cuts at: 4859 4859 Size: 7507 ScaI AGT'ACT Cuts at: 3625 4062 5755 7467 3625 Size: 437 1693 1712 3665 Fragments arranged by size: 3665 1712 1693 437 SfcI C'TryA_G Cuts at: 826 1045 2030 2228 3627 4855 5990 6671 6862 Size: 219 985 198 1399 1228 1135 681 191 Cuts at: 6862 826 Size: 1471 Fragments arranged by size: 1471 1399 1228 1135 985 681 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7507 SmaI CCC'GGG Cuts at: 1872 2168 4834 1872 Size: 296 2666 4545 Fragments arranged by size: 4545 2666 296 SmlI C'TyrA_G Cuts at: 820 1017 1943 2564 3012 4556 5611 6512 6774 Size: 197 926 621 448 1544 1055 901 262 Cuts at: 6774 820 Size: 1553 Fragments arranged by size: 1553 1544 1055 926 901 621 448 262 197 SnaBI TAC'GTA Cuts at: 493 5298 493 Size: 4805 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7507 SphI G_CATG'C Cuts at: 1132 2083 3923 4865 1132 Size: 951 1840 942 3774 Fragments arranged by size: 3774 1840 951 942 SspI AAT'ATT Cuts at: 5 52 3754 5431 5 Size: 47 3702 1677 2081 Fragments arranged by size: 3702 2081 1677 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7507 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 4371 4671 4946 513 Size: 552 939 336 2031 300 275 3074 Fragments arranged by size: 3074 2031 939 552 336 300 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 4068 5534 5551 5704 5889 6334 Size: 1255 858 1630 1466 17 153 185 445 Cuts at: 6334 325 Size: 1498 Fragments arranged by size: 1630 1498 1466 1255 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 3623 4060 Size: 274 80 33 51 1443 222 1424 437 Cuts at: 4060 5753 7465 96 Size: 1693 1712 138 Fragments arranged by size: 1712 1693 1443 1424 437 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7467 7499 7467 Size: 32 7475 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7507 XbaI T'CTAG_A Cuts at: 4843 4843 Size: 7507 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 2483 4004 2443 Size: 40 1521 5946 Fragments arranged by size: 5946 1521 40 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7507 XmaI C'CCGG_G Cuts at: 1870 2166 4832 1870 Size: 296 2666 4545 Fragments arranged by size: 4545 2666 296 XmnI GAAnn'nnTTC Cuts at: 2403 5636 2403 Size: 3233 4274 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5315 276 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI