(Circular) (Six-base) MAPSORT of: pFN21AB9453.seq Check: 2156 from: 1 to: 6622 pFN21AB9453 6622 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2389 2389 Size: 6622 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 3965 1937 Size: 1146 882 4594 Fragments arranged by size: 4594 1146 882 Acc65I G'GTAC_C Cuts at: 3943 3943 Size: 6622 AclI AA'CG_TT Cuts at: 1563 3921 4749 5122 1563 Size: 2358 828 373 3063 Fragments arranged by size: 3063 2358 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 2702 2856 3108 4685 6069 6337 1632 Size: 884 186 154 252 1577 1384 268 1917 Fragments arranged by size: 1917 1577 1384 884 268 252 186 154 AfeI AGC'GCT Cuts at: 3610 3610 Size: 6622 AflII C'TTAA_G Cuts at: 820 1017 2788 820 Size: 197 1771 4654 Fragments arranged by size: 4654 1771 197 AflIII A'CryG_T Cuts at: 1203 2405 2407 5521 6498 1203 Size: 1202 2 3114 977 1327 Fragments arranged by size: 3114 1327 1202 977 2 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 5351 5351 Size: 6622 AleI CACnn'nnGTG Cuts at: 1166 2834 3701 1166 Size: 1668 867 4087 Fragments arranged by size: 4087 1668 867 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3669 1184 Size: 2485 4137 AlwNI CAG_nnn'CTG Cuts at: 2987 3355 4365 5937 2987 Size: 368 1010 1572 3672 Fragments arranged by size: 3672 1572 1010 368 ApaI G_GGCC'C Cuts at: 2481 3174 3311 3748 2481 Size: 693 137 437 5355 Fragments arranged by size: 5355 693 437 137 ApaLI G'TGCA_C Cuts at: 4679 5835 4679 Size: 1156 5466 ApoI r'AATT_y Cuts at: 1493 2882 3112 3931 4157 4309 6418 1493 Size: 1389 230 819 226 152 2109 1697 Fragments arranged by size: 2109 1697 1389 819 230 226 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2292 3716 3947 1870 Size: 73 196 153 1424 231 4545 Fragments arranged by size: 4545 1424 231 196 153 73 BamHI G'GATC_C Cuts at: 3952 4319 3952 Size: 367 6255 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3324 3703 3943 5398 618 Size: 325 217 627 1537 379 240 1455 1842 Fragments arranged by size: 1842 1537 1455 627 379 325 240 217 BanII G_rGCy'C Cuts at: 1391 2259 2481 2807 2996 3174 3311 3377 3676 Size: 868 222 326 189 178 137 66 299 Cuts at: 3676 3748 3941 1391 Size: 72 193 4072 Fragments arranged by size: 4072 868 326 299 222 193 189 178 137 72 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2099 2187 2778 928 Size: 971 200 88 591 4772 Fragments arranged by size: 4772 971 591 200 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4813 4847 1903 Size: 34 2876 34 3678 Fragments arranged by size: 3678 2876 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4514 5724 4514 Size: 1210 5412 BclI T'GATC_A Cuts at: 1548 1548 Size: 6622 BfrBI ATG'CAT Cuts at: 3151 3151 Size: 6622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5233 136 Size: 107 122 71 631 605 3561 1525 Fragments arranged by size: 3561 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6617 6617 Size: 6622 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 2481 3174 3311 3327 3708 3748 4683 5839 Size: 691 693 137 16 381 40 935 1156 Cuts at: 5839 1790 Size: 2573 Fragments arranged by size: 2573 1156 935 693 691 381 137 40 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2972 3453 4353 Size: 416 53 83 399 24 1555 481 900 Cuts at: 4353 5311 442 Size: 958 1753 Fragments arranged by size: 1753 1555 958 900 481 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2382 2685 3651 3876 5282 1239 Size: 1143 303 966 225 1406 2579 Fragments arranged by size: 2579 1406 1143 966 303 225 Bpu10I CC'TnA_GC Cuts at: 2253 2913 2253 Size: 660 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4747 5612 5910 4747 Size: 865 298 5459 Fragments arranged by size: 5459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2202 2804 5285 882 Size: 617 703 602 2481 2219 Fragments arranged by size: 2481 2219 703 617 602 BsaAI yAC'GTr Cuts at: 493 1204 2408 4413 493 Size: 711 1204 2005 2702 Fragments arranged by size: 2702 2005 1204 711 BsaBI GATnn'nnATC Cuts at: 2531 6616 2531 Size: 4085 2537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4429 4811 5446 275 Size: 53 83 186 936 2896 382 635 1451 Fragments arranged by size: 2896 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2012 2365 5055 5453 5727 5874 6285 2012 Size: 353 2690 398 274 147 411 2349 Fragments arranged by size: 2690 2349 411 398 353 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6397 Fragments arranged by size: 6397 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 2339 3327 3798 3801 1186 Size: 888 265 988 471 3 4007 Fragments arranged by size: 4007 988 888 471 265 3 BseYI C'CCAG_C Cuts at: 2980 3209 3542 5825 6321 2980 Size: 229 333 2283 496 3281 Fragments arranged by size: 3281 2283 496 333 229 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6049 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 4072 4833 5861 1540 Size: 275 186 12 2059 761 1028 2301 Fragments arranged by size: 2301 2059 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2807 4683 4768 5839 2807 Size: 1876 85 1071 3590 Fragments arranged by size: 3590 1876 1071 85 BsmI GAATG_Cn' Cuts at: 2115 4133 4226 2115 Size: 2018 93 4511 Fragments arranged by size: 4511 2018 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3078 3078 Size: 6622 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2481 2807 2996 3174 3311 3327 Size: 399 469 222 326 189 178 137 16 Cuts at: 3327 3377 3676 3708 3748 3941 4683 4768 5839 Size: 50 299 32 40 193 742 85 1071 Cuts at: 5839 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 469 399 326 299 222 193 189 178 137 85 50 40 32 16 BspEI T'CCGG_A Cuts at: 2365 6285 2365 Size: 3920 2702 BspHI T'CATG_A Cuts at: 2424 4511 6241 2424 Size: 2087 1730 2805 Fragments arranged by size: 2805 2087 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2389 3977 844 Size: 1545 1588 3489 Fragments arranged by size: 3489 1588 1545 BsrBI CCG'CTC Cuts at: 1394 1823 2329 4509 1394 Size: 429 506 2180 3507 Fragments arranged by size: 3507 2180 506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2524 2865 5117 5291 65 Size: 1183 1276 341 2252 174 1396 Fragments arranged by size: 2252 1396 1276 1183 341 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3782 5266 5453 1700 Size: 110 1972 1484 187 2869 Fragments arranged by size: 2869 1972 1484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 3604 4682 5694 3604 Size: 1078 1012 4532 Fragments arranged by size: 4532 1078 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6622 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6622 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2638 2894 3022 3273 3320 2032 Size: 606 256 128 251 47 5334 Fragments arranged by size: 5334 606 256 251 128 47 BstYI r'GATC_y Cuts at: 1972 2784 3952 4319 4704 4721 6173 6259 6271 Size: 812 1168 367 385 17 1452 86 12 Cuts at: 6271 6617 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1168 812 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2232 2232 Size: 6622 BtgI C'CryG_G Cuts at: 513 1065 2004 3142 3328 3500 3575 3632 3822 Size: 552 939 1138 186 172 75 57 190 Cuts at: 3822 513 Size: 3313 Fragments arranged by size: 3313 1138 939 552 190 186 172 75 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5883 Fragments arranged by size: 5883 683 56 BtsI GCAGTG_nn' Cuts at: 2664 4138 4932 4952 2664 Size: 1474 794 20 4334 Fragments arranged by size: 4334 1474 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6587 DraI TTT'AAA Cuts at: 3926 3926 Size: 6622 DraIII CAC_nnn'GTG Cuts at: 2838 2838 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2581 2860 3024 Size: 54 1425 50 275 18 751 279 164 Cuts at: 3024 4069 4958 6587 8 Size: 1045 889 1629 43 Fragments arranged by size: 1629 1425 1045 889 751 279 275 164 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2316 3498 4552 1353 Size: 698 265 1182 1054 3423 Fragments arranged by size: 3423 1182 1054 698 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3516 3528 3747 3769 3804 5209 Size: 1064 138 1927 12 219 22 35 1405 Cuts at: 5209 5583 5729 387 Size: 374 146 1280 Fragments arranged by size: 1927 1405 1280 1064 374 219 146 138 35 22 12 EcoICRI GAG'CTC Cuts at: 2805 2805 Size: 6622 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 2516 2685 2702 2856 3108 3651 Size: 393 750 134 169 17 154 252 543 Cuts at: 3651 3876 4685 5282 6069 6337 1239 Size: 225 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 750 597 543 393 268 252 225 169 154 134 17 EcoNI CCTnn'n_nnAGG Cuts at: 2684 2684 Size: 6622 EcoO109I rG'GnC_Cy Cuts at: 2241 2760 3307 3308 3744 3745 2241 Size: 519 547 1 436 1 5118 Fragments arranged by size: 5118 547 519 436 1 1 EcoRI G'AATT_C Cuts at: 3112 3931 4309 3112 Size: 819 378 5425 Fragments arranged by size: 5425 819 378 FspI TGC'GCA Cuts at: 2201 5128 2201 Size: 2927 3695 HaeII r_GCGC'y Cuts at: 1678 3612 5769 1678 Size: 1934 2157 2531 Fragments arranged by size: 2531 2157 1934 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2891 2923 5263 Size: 32 135 32 653 32 634 32 2340 Cuts at: 5263 5295 5337 5369 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2340 653 634 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3966 4212 669 Size: 988 281 2028 246 3079 Fragments arranged by size: 3079 2028 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6622 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KpnI G_GTAC'C Cuts at: 3947 3947 Size: 6622 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2013 5736 5920 1305 Size: 708 3723 184 2007 Fragments arranged by size: 3723 2007 708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2583 2862 3026 10 Size: 54 1425 343 751 279 164 3606 Fragments arranged by size: 3606 1425 751 343 279 164 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2834 3156 3701 3848 3860 4580 Size: 648 1182 486 322 545 147 12 720 Cuts at: 4580 4939 5098 518 Size: 359 159 2042 Fragments arranged by size: 2042 1182 720 648 545 486 359 322 159 147 12 MspA1I CmG'CkG Cuts at: 1606 2067 4038 4715 5863 6108 1606 Size: 461 1971 677 1148 245 2120 Fragments arranged by size: 2120 1971 1148 677 461 245 NaeI GCC'GGC Cuts at: 3784 3784 Size: 6622 NcoI C'CATG_G Cuts at: 513 1065 2004 3328 3500 3575 3632 3822 513 Size: 552 939 1324 172 75 57 190 3313 Fragments arranged by size: 3313 1324 939 552 190 172 75 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NgoMIV G'CCGG_C Cuts at: 3782 3782 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NsiI A_TGCA'T Cuts at: 3153 3153 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 3151 3874 3980 5525 1132 Size: 97 1922 723 106 1545 2229 Fragments arranged by size: 2229 1922 1545 723 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6622 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 3773 1108 Size: 2665 3957 PfoI T'CCnGG_A Cuts at: 2104 3667 2104 Size: 1563 5059 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PmlI CAC'GTG Cuts at: 2408 2408 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2241 2760 2241 Size: 519 6103 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PspOMI G'GGCC_C Cuts at: 2477 3170 3307 3744 2477 Size: 693 137 437 5355 Fragments arranged by size: 5355 693 437 137 PstI C_TGCA'G Cuts at: 830 2175 2386 3974 830 Size: 1345 211 1588 3478 Fragments arranged by size: 3478 1588 1345 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 SacI G_AGCT'C Cuts at: 2807 2807 Size: 6622 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SanDI GG'GwC_CC Cuts at: 2760 2760 Size: 6622 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SfcI C'TryA_G Cuts at: 826 1045 2115 2171 2382 3970 5105 5786 5977 Size: 219 1070 56 211 1588 1135 681 191 Cuts at: 5977 826 Size: 1471 Fragments arranged by size: 1588 1471 1135 1070 681 219 211 191 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3718 3949 1872 Size: 1846 231 4545 Fragments arranged by size: 4545 1846 231 SmlI C'TyrA_G Cuts at: 820 1017 1943 2139 2788 4726 5627 5889 820 Size: 197 926 196 649 1938 901 262 1553 Fragments arranged by size: 1938 1553 926 901 649 262 197 196 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3151 3874 3980 1132 Size: 2019 723 106 3774 Fragments arranged by size: 3774 2019 723 106 SspI AAT'ATT Cuts at: 5 52 2942 4546 5 Size: 47 2890 1604 2081 Fragments arranged by size: 2890 2081 1604 47 StuI AGG'CCT Cuts at: 1517 2893 1517 Size: 1376 5246 StyI C'CwwG_G Cuts at: 513 1065 2004 3272 3328 3500 3575 3632 3822 Size: 552 939 1268 56 172 75 57 190 Cuts at: 3822 4061 513 Size: 239 3074 Fragments arranged by size: 3074 1268 939 552 239 190 172 75 57 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2954 3104 3216 4649 4666 4819 5004 Size: 1255 1374 150 112 1433 17 153 185 Cuts at: 5004 5449 325 Size: 445 1498 Fragments arranged by size: 1498 1433 1374 1255 445 185 153 150 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4868 6580 96 Size: 274 80 33 51 1443 2891 1712 138 Fragments arranged by size: 2891 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6582 6614 6582 Size: 32 6590 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3019 3149 3550 3853 3019 Size: 130 401 303 5788 Fragments arranged by size: 5788 401 303 130 XhoI C'TCGA_G Cuts at: 1943 2139 1943 Size: 196 6426 XmaI C'CCGG_G Cuts at: 1870 3716 3947 1870 Size: 1846 231 4545 Fragments arranged by size: 4545 1846 231 XmnI GAAnn'nnTTC Cuts at: 4751 4751 Size: 6622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI