Restriction map for
pFN21AB8168
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI Ksp632I KspI
MaeII MamI MfeI MluI MseI
MunI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 225 GGTACC G^GTACC
AccIII 669 TCCGGA T^CCGGA
AgeI 714 ACCGGT A^CCGGT
Asp718I 225 GGTACC G^GTACC
AvaI 16 CCCGAG C^[CT]CG[GA]G
BalI 143 TGGCCA TGG^CCA
BlpI 256 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 256 GCTAAGC GC^T[ACGT]AGC
BseAI 669 TCCGGA T^CCGGA
BsmI 385 GAATGC GAATGC(1/-1)
BsoBI 16 CCCGAG C^[CT]CG[GA]G
BspEI 669 TCCGGA T^CCGGA
BsrBI 504 GAGCGG CCGCTC(-3/-3)
BsrFI 714 ACCGGT [GA]^CCGG[CT]
BstAPI 394 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 729 GGTCACC G^GT[ACGT]ACC
BstF5I 667 CATCC GGATG(2/0)
BstPI 729 GGTCACC G^GT[ACGT]ACC
BstXI 53 CCATCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 256 GCTAAGC GC^T[ACGT]AGC
Cfr10I 714 ACCGGT [GA]^CCGG[CT]
CpoI 711 CGGACCG CG^G[AT]CCG
EcoO65I 729 GGTCACC G^GT[ACGT]ACC
FokI 667 CATCC GGATG(9/13)
HgaI 340 GACGC GACGC(5/10)
KpnI 225 GGTACC GGTAC^C
MluNI 143 TGGCCA TGG^CCA
MroI 669 TCCGGA T^CCGGA
MscI 143 TGGCCA TGG^CCA
MslI 54 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 114 CATG CATG^
PflMI 135 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 714 ACCGGT A^CCGGT
PstI 280 CTGCAG CTGCA^G
RsrII 711 CGGACCG CG^G[AT]CCG
SacI 8 GAGCTC GAGCT^C
SmlI 93 CTTGAG C^T[CT][GA]AG
Tsp45I 730 GTCAC ^GT[GC]AC
Tsp509I 168 AATT ^AATT
Van91I 135 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 508 GGCGCC G[GA]^CG[CT]C
675 GGCGCC
AfaI 21 GTAC GT^AC
226 GTAC
AspHI 8 GAGCTC G[AT]GC[AT]^C
718 GTGCTC
AvaII 542 GGACC G^G[AT]CC
712 GGACC
BbeI 508 GGCGCC GGCGC^C
675 GGCGCC
BbsI 547 GTCTTC GAAGAC(2/6)
787 GTCTTC
BpuAI 547 GTCTTC GAAGAC(2/6)
787 GTCTTC
BsaHI 508 GGCGCC G[GA]^CG[CT]C
675 GGCGCC
BsaI 49 GAGACC GGTCTC(1/5)
185 GAGACC
BseRI 27 CTCCTC GAGGAG(10/8)
765 CTCCTC
BsiHKAI 8 GAGCTC G[AT]GC[AT]^C
718 GTGCTC
BsrI 459 ACTGG ACTGG(1/-1)
230 CCAGT
BstYI 250 GGATCT [GA]^GATC[CT]
275 AGATCC
EaeI 143 TGGCCA [CT]^GGCC[GA]
597 CGGCCA
EcoT14I 867 CCTTGG C^C[AT][AT]GG
886 CCTTGG
Hin1I 508 GGCGCC G[GA]^CG[CT]C
675 GGCGCC
KasI 508 GGCGCC G^GCGCC
675 GGCGCC
MaeIII 332 GTTAC ^GT[ACGT]AC
730 GTCAC
MflI 250 GGATCT [GA]^GATC[CT]
275 AGATCC
MspA1I 490 CAGCGG C[AC]G^C[GT]G
577 CCGCTG
NarI 508 GGCGCC GG^CGCC
675 GGCGCC
RsaI 21 GTAC GT^AC
226 GTAC
SfcI 280 CTGCAG C^T[GA][CT]AG
879 CTATAG
SfoI 508 GGCGCC GGC^GCC
675 GGCGCC
StyI 867 CCTTGG C^C[AT][AT]GG
886 CCTTGG
XhoII 250 GGATCT [GA]^GATC[CT]
275 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AlwI BanI
BanII BbvI BfaI BmyI BsaJI
BsaWI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I