Restriction map for
pFN21AB8168
(Linear) (Six-base) MAPSORT of: KIBB8168.seq Check: 9658 from: 1 to: 912
KIBB8168.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
Acc65I G'GTAC_C
Cuts at: 0 225 912
Size: 225 687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 912
Size: 827 85
AgeI A'CCGG_T
Cuts at: 0 714 912
Size: 714 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 269 368 912
Size: 269 99 544
Fragments arranged by size:
544 269 99
AvaI C'yCGr_G
Cuts at: 0 16 912
Size: 16 896
BanI G'GyrC_C
Cuts at: 0 225 508 613 675 912
Size: 225 283 105 62 237
Fragments arranged by size:
283 237 225 105 62
BanII G_rGCy'C
Cuts at: 0 12 661 841 912
Size: 12 649 180 71
Fragments arranged by size:
649 180 71 12
BbeI G_GCGC'C
Cuts at: 0 512 679 912
Size: 512 167 233
Fragments arranged by size:
512 233 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 540 780 912
Size: 540 240 132
Fragments arranged by size:
540 240 132
BlpI GC'TnA_GC
Cuts at: 0 257 912
Size: 257 655
Bme1580I G_kGCm'C
Cuts at: 0 616 912
Size: 616 296
BmrI ACTGGGnnnn_n'
Cuts at: 0 224 912
Size: 224 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 119 912
Size: 42 77 793
Fragments arranged by size:
793 77 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 912
Size: 114 798
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 179 912
Size: 43 136 733
Fragments arranged by size:
733 136 43
BsaHI Gr'CG_yC
Cuts at: 0 509 676 912
Size: 509 167 236
Fragments arranged by size:
509 236 167
BsaWI w'CCGG_w
Cuts at: 0 188 669 714 912
Size: 188 481 45 198
Fragments arranged by size:
481 198 188 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 704 734 912
Size: 704 30 178
Fragments arranged by size:
704 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 756 912
Size: 18 738 156
Fragments arranged by size:
738 156 18
BseYI C'CCAG_C
Cuts at: 0 488 654 912
Size: 488 166 258
Fragments arranged by size:
488 258 166
BsiHKAI G_wGCw'C
Cuts at: 0 12 722 912
Size: 12 710 190
Fragments arranged by size:
710 190 12
BsmI GAATG_Cn'
Cuts at: 0 391 912
Size: 391 521
Bsp1286I G_dGCh'C
Cuts at: 0 12 616 661 722 841 912
Size: 12 604 45 61 119 71
Fragments arranged by size:
604 119 71 61 45 12
BspEI T'CCGG_A
Cuts at: 0 669 912
Size: 669 243
BsrBI CCG'CTC
Cuts at: 0 506 912
Size: 506 406
BsrFI r'CCGG_y
Cuts at: 0 714 912
Size: 714 198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 912
Size: 400 512
BstEII G'GTnAC_C
Cuts at: 0 729 912
Size: 729 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 912
Size: 60 852
BstYI r'GATC_y
Cuts at: 0 250 275 912
Size: 250 25 637
Fragments arranged by size:
637 250 25
BtgI C'CryG_G
Cuts at: 0 290 600 617 629 912
Size: 290 310 17 12 283
Fragments arranged by size:
310 290 283 17 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 552 912
Size: 552 360
EaeI y'GGCC_r
Cuts at: 0 143 597 912
Size: 143 454 315
Fragments arranged by size:
454 315 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 726 912
Size: 726 186
EcoICRI GAG'CTC
Cuts at: 0 10 912
Size: 10 902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 912
Size: 827 85
HaeII r_GCGC'y
Cuts at: 0 180 512 679 694 912
Size: 180 332 167 15 218
Fragments arranged by size:
332 218 180 167 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 279 311 704 736 912
Size: 176 32 71 32 393 32 176
Fragments arranged by size:
393 176 176 71 32 32 32
KasI G'GCGC_C
Cuts at: 0 508 675 912
Size: 508 167 237
Fragments arranged by size:
508 237 167
KpnI G_GTAC'C
Cuts at: 0 229 912
Size: 229 683
MscI TGG'CCA
Cuts at: 0 145 912
Size: 145 767
MslI CAynn'nnrTG
Cuts at: 0 58 912
Size: 58 854
MspA1I CmG'CkG
Cuts at: 0 492 579 912
Size: 492 87 333
Fragments arranged by size:
492 333 87
NarI GG'CG_CC
Cuts at: 0 509 676 912
Size: 509 167 236
Fragments arranged by size:
509 236 167
PasI CC'CwG_GG
Cuts at: 0 536 912
Size: 536 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 912
Size: 141 771
PstI C_TGCA'G
Cuts at: 0 284 912
Size: 284 628
RsrII CG'GwC_CG
Cuts at: 0 712 912
Size: 712 200
SacI G_AGCT'C
Cuts at: 0 12 912
Size: 12 900
SfcI C'TryA_G
Cuts at: 0 280 879 912
Size: 280 599 33
Fragments arranged by size:
599 280 33
SfoI GGC'GCC
Cuts at: 0 510 677 912
Size: 510 167 235
Fragments arranged by size:
510 235 167
SmlI C'TyrA_G
Cuts at: 0 93 912
Size: 93 819
StyI C'CwwG_G
Cuts at: 0 867 886 912
Size: 867 19 26
Fragments arranged by size:
867 26 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 774 864 912
Size: 642 132 90 48
Fragments arranged by size:
642 132 90 48
TatI w'GTAC_w
Cuts at: 0 20 912
Size: 20 892
Enzymes that do cut:
Acc65I AcuI AgeI AlfI AvaI BanI BanII BbeI
BbsI BlpI Bme1580I BmrI BplI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
EaeI EciI EcoICRI Eco57MI HaeII Hin4I KasI KpnI
MscI MslI MspA1I NarI PasI PflMI PstI RsrII
SacI SfcI SfoI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BpmI Bpu10I BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI