(Circular) (Six-base) MAPSORT of: pFN21AE3522.seq Check: 1189 from: 1 to: 6841 pFN21AE3522 6841 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4651 278 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2444 2669 4184 1937 Size: 507 225 1515 4594 Fragments arranged by size: 4594 1515 507 225 Acc65I G'GTAC_C Cuts at: 3332 4162 3332 Size: 830 6011 AclI AA'CG_TT Cuts at: 1563 4968 5341 1563 Size: 3405 373 3063 Fragments arranged by size: 3405 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2852 3606 3957 4904 6288 6556 1632 Size: 677 543 754 351 947 1384 268 1917 Fragments arranged by size: 1917 1384 947 754 677 543 351 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6644 AflIII A'CryG_T Cuts at: 1203 2029 3437 5740 6717 1203 Size: 826 1408 2303 977 1327 Fragments arranged by size: 2303 1408 1327 977 826 AgeI A'CCGG_T Cuts at: 5672 5672 Size: 6841 AhdI GACnn_n'nnGTC Cuts at: 5570 5570 Size: 6841 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6841 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3933 1184 Size: 2749 4092 AlwNI CAG_nnn'CTG Cuts at: 3584 4584 6156 3584 Size: 1000 1572 4269 Fragments arranged by size: 4269 1572 1000 ApaLI G'TGCA_C Cuts at: 3982 4898 6054 3982 Size: 916 1156 4769 Fragments arranged by size: 4769 1156 916 ApoI r'AATT_y Cuts at: 1493 2833 2857 3281 3572 4150 4376 4528 6637 Size: 1340 24 424 291 578 226 152 2109 Cuts at: 6637 1493 Size: 1697 Fragments arranged by size: 2109 1697 1340 578 424 291 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 5395 160 Size: 872 4363 1606 Fragments arranged by size: 4363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 AvaI C'yCGr_G Cuts at: 1870 1943 4166 1870 Size: 73 2223 4545 Fragments arranged by size: 4545 2223 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2979 3012 2979 Size: 33 6808 BamHI G'GATC_C Cuts at: 4171 4538 4171 Size: 367 6474 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2408 2698 3332 4162 5617 Size: 325 217 627 621 290 634 830 1455 Cuts at: 5617 618 Size: 1842 Fragments arranged by size: 1842 1455 830 634 627 621 325 290 217 BanII G_rGCy'C Cuts at: 1391 2661 3810 4080 4160 1391 Size: 1270 1149 270 80 4072 Fragments arranged by size: 4072 1270 1149 270 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2960 4113 928 Size: 971 1061 1153 3656 Fragments arranged by size: 3656 1153 1061 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5032 5066 1903 Size: 34 50 34 3011 34 3678 Fragments arranged by size: 3678 3011 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2011 2463 4733 5943 2011 Size: 452 2270 1210 2909 Fragments arranged by size: 2909 2270 1210 452 BclI T'GATC_A Cuts at: 1548 1548 Size: 6841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5452 136 Size: 107 122 71 631 605 3780 1525 Fragments arranged by size: 3780 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2960 6836 2960 Size: 3876 2965 BlpI GC'TnA_GC Cuts at: 4258 4258 Size: 6841 Bme1580I G_kGCm'C Cuts at: 1790 2413 3912 3986 4902 6058 1790 Size: 623 1499 74 916 1156 2573 Fragments arranged by size: 2573 1499 1156 916 623 74 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6841 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4572 5530 442 Size: 416 53 83 399 24 3155 958 1753 Fragments arranged by size: 3155 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2012 2949 1056 Size: 956 937 4948 Fragments arranged by size: 4948 956 937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 3028 4056 5501 1239 Size: 1062 727 1028 1445 2579 Fragments arranged by size: 2579 1445 1062 1028 727 Bpu10I CC'TnA_GC Cuts at: 2129 3276 4136 2129 Size: 1147 860 4834 Fragments arranged by size: 4834 1147 860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2037 2162 3993 4966 5831 6129 2037 Size: 125 1831 973 865 298 2749 Fragments arranged by size: 2749 1831 973 865 298 125 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2277 3208 3818 5504 882 Size: 617 778 931 610 1686 2219 Fragments arranged by size: 2219 1686 931 778 617 610 BsaAI yAC'GTr Cuts at: 493 1204 2022 3610 4632 493 Size: 711 818 1588 1022 2702 Fragments arranged by size: 2702 1588 1022 818 711 BsaBI GATnn'nnATC Cuts at: 3901 6835 3901 Size: 2934 3907 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4648 5030 5665 275 Size: 53 83 186 936 3115 382 635 1451 Fragments arranged by size: 3115 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5274 5672 5946 6093 6504 5274 Size: 398 274 147 411 5611 Fragments arranged by size: 5611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6616 Fragments arranged by size: 6616 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2773 1186 Size: 1587 5254 BseYI C'CCAG_C Cuts at: 3540 6044 6540 3540 Size: 2504 496 3841 Fragments arranged by size: 3841 2504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2323 3704 1163 Size: 573 587 1381 4300 Fragments arranged by size: 4300 1381 587 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2109 4291 5052 6080 1540 Size: 275 186 108 2182 761 1028 2301 Fragments arranged by size: 2301 2182 1028 761 275 186 108 BsiHKAI G_wGCw'C Cuts at: 3986 4080 4902 4987 6058 3986 Size: 94 822 85 1071 4769 Fragments arranged by size: 4769 1071 822 94 85 BsmI GAATG_Cn' Cuts at: 3854 4352 4445 3854 Size: 498 93 6250 Fragments arranged by size: 6250 498 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2413 2661 3810 3912 3986 4080 4160 Size: 399 623 248 1149 102 74 94 80 Cuts at: 4160 4902 4987 6058 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1149 1071 742 623 399 248 102 94 85 80 74 BspEI T'CCGG_A Cuts at: 6504 6504 Size: 6841 BspHI T'CATG_A Cuts at: 3151 4730 6460 3151 Size: 1579 1730 3532 Fragments arranged by size: 3532 1730 1579 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4196 844 Size: 3352 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4728 1394 Size: 429 2905 3507 Fragments arranged by size: 3507 2905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2019 3967 5336 5510 65 Size: 1183 771 1948 1369 174 1396 Fragments arranged by size: 1948 1396 1369 1183 771 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5485 5672 1700 Size: 110 3675 187 2869 Fragments arranged by size: 3675 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6841 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6841 BssSI C'ACGA_G Cuts at: 4901 5913 4901 Size: 1012 5829 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6841 BstBI TT'CG_AA Cuts at: 2491 5645 2491 Size: 3154 3687 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6841 BstYI r'GATC_y Cuts at: 1972 2960 4171 4538 4923 4940 6392 6478 6490 Size: 988 1211 367 385 17 1452 86 12 Cuts at: 6490 6836 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1211 988 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2445 2445 Size: 6841 Bsu36I CC'TnA_GG Cuts at: 3705 3705 Size: 6841 BtgI C'CryG_G Cuts at: 513 1065 2370 3870 3886 513 Size: 552 1305 1500 16 3468 Fragments arranged by size: 3468 1500 1305 552 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6102 Fragments arranged by size: 6102 683 56 BtsI GCAGTG_nn' Cuts at: 2335 3526 3915 3955 4357 5151 5171 2335 Size: 1191 389 40 402 794 20 4005 Fragments arranged by size: 4005 1191 794 402 389 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3161 3196 529 Size: 35 2597 35 4174 Fragments arranged by size: 4174 2597 35 35 DraI TTT'AAA Cuts at: 2910 4145 2910 Size: 1235 5606 DraIII CAC_nnn'GTG Cuts at: 2092 3229 2092 Size: 1137 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3054 5848 809 Size: 507 1738 2794 1802 Fragments arranged by size: 2794 1802 1738 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3567 4288 5177 Size: 54 1425 50 275 18 1737 721 889 Cuts at: 5177 6806 8 Size: 1629 43 Fragments arranged by size: 1737 1629 1425 889 721 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4288 1537 Size: 275 2476 4090 Fragments arranged by size: 4090 2476 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 4771 1353 Size: 807 2611 3423 Fragments arranged by size: 3423 2611 807 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5428 5802 5948 387 Size: 1064 138 3839 374 146 1280 Fragments arranged by size: 3839 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4078 4078 Size: 6841 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2309 2852 3028 3606 3957 4056 Size: 393 669 8 543 176 578 351 99 Cuts at: 4056 4904 5501 6288 6556 1239 Size: 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 669 597 578 543 393 351 268 176 99 8 EcoNI CCTnn'n_nnAGG Cuts at: 3704 3704 Size: 6841 EcoRI G'AATT_C Cuts at: 2857 4150 4528 2857 Size: 1293 378 5170 Fragments arranged by size: 5170 1293 378 FspI TGC'GCA Cuts at: 5347 5347 Size: 6841 HaeII r_GCGC'y Cuts at: 1678 5988 1678 Size: 4310 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3443 3475 3708 3740 4081 Size: 32 135 32 1871 32 233 32 341 Cuts at: 4081 4106 4113 4138 5482 5514 5556 5588 1373 Size: 25 7 25 1344 32 42 32 2626 Fragments arranged by size: 2626 1871 1344 341 233 135 42 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2670 4185 4431 669 Size: 988 281 732 1515 246 3079 Fragments arranged by size: 3079 1515 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 2912 3588 4011 748 Size: 2164 676 423 3578 Fragments arranged by size: 3578 2164 676 423 HpaI GTT'AAC Cuts at: 4431 4431 Size: 6841 KpnI G_GTAC'C Cuts at: 3336 4166 3336 Size: 830 6011 MfeI C'AATT_G Cuts at: 4440 4440 Size: 6841 MluI A'CGCG_T Cuts at: 6717 6717 Size: 6841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5955 6139 1305 Size: 4650 184 2007 Fragments arranged by size: 4650 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3569 10 Size: 54 1425 343 1737 3282 Fragments arranged by size: 3282 1737 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2406 3960 4101 4799 5158 5317 518 Size: 648 1240 1554 141 698 359 159 2042 Fragments arranged by size: 2042 1554 1240 698 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2041 3872 4257 4934 6082 6327 1606 Size: 435 1831 385 677 1148 245 2120 Fragments arranged by size: 2120 1831 1148 677 435 385 245 NcoI C'CATG_G Cuts at: 513 1065 2370 3886 513 Size: 552 1305 1516 3468 Fragments arranged by size: 3468 1516 1305 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6841 NheI G'CTAG_C Cuts at: 1052 2008 2945 1052 Size: 956 937 4948 Fragments arranged by size: 4948 956 937 NotI GC'GGCC_GC Cuts at: 4288 4288 Size: 6841 NspI r_CATG'y Cuts at: 1132 1229 2033 2174 3441 3450 3982 4199 5744 Size: 97 804 141 1267 9 532 217 1545 Cuts at: 5744 1132 Size: 2229 Fragments arranged by size: 2229 1545 1267 804 532 217 141 97 9 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6841 PciI A'CATG_T Cuts at: 2029 3437 5740 2029 Size: 1408 2303 3130 Fragments arranged by size: 3130 2303 1408 PflMI CCAn_nnn'nTGG Cuts at: 1108 2932 1108 Size: 1824 5017 PmeI GTTT'AAAC Cuts at: 4145 4145 Size: 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4910 4942 6423 6455 4910 Size: 32 1481 32 5296 Fragments arranged by size: 5296 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6841 PsiI TTA'TAA Cuts at: 4411 4411 Size: 6841 PstI C_TGCA'G Cuts at: 830 2046 3821 4193 830 Size: 1216 1775 372 3478 Fragments arranged by size: 3478 1775 1216 372 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6841 PvuII CAG'CTG Cuts at: 2041 2041 Size: 6841 SacI G_AGCT'C Cuts at: 4080 4080 Size: 6841 SacII CC_GC'GG Cuts at: 3873 3873 Size: 6841 SalI G'TCGA_C Cuts at: 1936 2668 4183 1936 Size: 732 1515 4594 Fragments arranged by size: 4594 1515 732 SapI GCTCTTCn'nnn_ Cuts at: 2160 2160 Size: 6841 SbfI CC_TGCA'GG Cuts at: 4193 4193 Size: 6841 ScaI AGT'ACT Cuts at: 2739 5089 6801 2739 Size: 2350 1712 2779 Fragments arranged by size: 2779 2350 1712 SfcI C'TryA_G Cuts at: 826 1045 2042 3817 4189 5324 6005 6196 826 Size: 219 997 1775 372 1135 681 191 1471 Fragments arranged by size: 1775 1471 1135 997 681 372 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6841 SmaI CCC'GGG Cuts at: 1872 4168 1872 Size: 2296 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2052 2177 4008 4945 5846 6108 Size: 197 926 109 125 1831 937 901 262 Cuts at: 6108 820 Size: 1553 Fragments arranged by size: 1831 1553 937 926 901 262 197 125 109 SnaBI TAC'GTA Cuts at: 493 3610 4632 493 Size: 3117 1022 2702 Fragments arranged by size: 3117 2702 1022 SpeI A'CTAG_T Cuts at: 152 152 Size: 6841 SphI G_CATG'C Cuts at: 1132 2174 4199 1132 Size: 1042 2025 3774 Fragments arranged by size: 3774 2025 1042 SspI AAT'ATT Cuts at: 5 52 4765 5 Size: 47 4713 2081 Fragments arranged by size: 4713 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6841 StyI C'CwwG_G Cuts at: 513 1065 2370 3886 4280 513 Size: 552 1305 1516 394 3074 Fragments arranged by size: 3074 1516 1305 552 394 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 2527 4868 4885 5038 5223 5668 Size: 1255 543 404 2341 17 153 185 445 Cuts at: 5668 325 Size: 1498 Fragments arranged by size: 2341 1498 1255 543 445 404 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2737 3135 3661 Size: 274 80 33 51 1443 760 398 526 Cuts at: 3661 5087 6799 96 Size: 1426 1712 138 Fragments arranged by size: 1712 1443 1426 760 526 398 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6801 6833 6801 Size: 32 6809 Tth111I GACn'n_nGTC Cuts at: 1316 2110 1316 Size: 794 6047 XbaI T'CTAG_A Cuts at: 4177 4177 Size: 6841 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2147 2011 Size: 136 6705 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6841 XmaI C'CCGG_G Cuts at: 1870 4166 1870 Size: 2296 4545 XmnI GAAnn'nnTTC Cuts at: 2541 3108 3643 4970 2541 Size: 567 535 1327 4412 Fragments arranged by size: 4412 1327 567 535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4649 276 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI