################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BanII BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI MamI MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp45I TspRI Tth111I Van91I XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 215 GGTACC G^GTACC AluI 758 AGCT AG^CT AlwNI 541 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 215 GGTACC G^GTACC BalI 458 TGGCCA TGG^CCA BcgI 597 CGAGGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 103 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 52 GTGCCC G[AGT]GC[ACT]^C BsaI 442 GAGACC GGTCTC(1/5) BsiEI 113 CGGCCG CG[GA][CT]^CG BsmFI 559 GGGAC GGGAC(10/14) Bsp1286I 52 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 506 TGTACA T^GTACA BspMI 418 ACCTGC ACCTGC(4/8) BsrGI 506 TGTACA T^GTACA BsrI 571 ACTGG ACTGG(1/-1) BssSI 447 CACGAG CACGAG(-5/-1) BstAPI 413 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DdeI 715 CTAAG C^T[ACGT]AG DraII 542 AGGCCCT [GA]G^G[ACGT]CC[CT] EagI 113 CGGCCG C^GGCCG EclXI 113 CGGCCG C^GGCCG Eco52I 113 CGGCCG C^GGCCG EcoO109I 542 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 92 CCTTGG C^C[AT][AT]GG HinfI 345 GACTC G^A[ACGT]TC HphI 131 TCACC GGTGA(8/7) KpnI 215 GGTACC GGTAC^C MaeII 587 ACGT A^CGT MaeIII 711 GTTAC ^GT[ACGT]AC MluNI 458 TGGCCA TGG^CCA MscI 458 TGGCCA TGG^CCA NlaIII 408 CATG CATG^ NotI 112 GCGGCCGC GC^GGCCGC PaeR7I 595 CTCGAG C^TCGAG PleI 345 GACTC GAGTC(4/5) PstI 435 CTGCAG CTGCA^G SapI 531 GAAGAGC GCTCTTC(1/4) SfaNI 144 GATGC GCATC(5/9) SfcI 435 CTGCAG C^T[GA][CT]AG SmaI 181 CCCGGG CCC^GGG SmlI 595 CTCGAG C^T[CT][GA]AG SspBI 506 TGTACA T^GTACA StyI 92 CCTTGG C^C[AT][AT]GG XbaI 330 TCTAGA T^CTAGA XcmI 446 CCACGAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 595 CTCGAG C^TCGAG XmaCI 181 CCCGGG C^CCGGG XmaI 181 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 216 GTAC GT^AC 507 GTAC AvaII 270 GGACC G^G[AT]CC 288 GGACC BseRI 247 GAGGAG GAGGAG(10/8) 340 GAGGAG BsgI 420 CTGCAC GTGCAG(16/14) 605 GTGCAG BsmAI 343 GAGAC GTCTC(1/5) 442 GAGAC BstF5I 237 GGATG GGATG(2/0) 479 GGATG BstYI 327 GGATCT [GA]^GATC[CT] 643 GGATCT EaeI 113 CGGCCG [CT]^GGCC[GA] 458 TGGCCA EarI 244 GAAGAG CTCTTC(1/4) 531 GAAGAG FokI 237 GGATG GGATG(9/13) 479 GGATG Ksp632I 244 GAAGAG CTCTTC(1/4) 531 GAAGAG KspI 62 CCGCGG CCGC^GG 116 CCGCGG MflI 327 GGATCT [GA]^GATC[CT] 643 GGATCT RsaI 216 GTAC GT^AC 507 GTAC SacII 62 CCGCGG CCGC^GG 116 CCGCGG TaqI 568 TCGA T^CGA 596 TCGA Tsp509I 128 AATT ^AATT 737 AATT TthHB8I 568 TCGA T^CGA 596 TCGA XhoII 327 GGATCT [GA]^GATC[CT] 643 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AcyI AlwI AvaI BanI BbeI BbvI BcnI BfaI BsaHI BsaJI BsiYI BslI BsoBI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DsaI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MaeI MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV Sau3AI Sau96I ScrFI SfoI Tru9I TseI