(Circular) (Six-base) MAPSORT of: pFN21AB5402.seq Check: 7055 from: 1 to: 5857 pFN21AB5402 5857 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2370 3200 1937 Size: 433 830 4594 Fragments arranged by size: 4594 830 433 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2882 3920 5304 5572 1632 Size: 1250 1038 1384 268 1917 Fragments arranged by size: 1917 1384 1250 1038 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 3091 4688 3091 Size: 1597 4260 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2608 1184 Size: 1424 4433 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2794 2826 2794 Size: 32 5825 AlwNI CAG_nnn'CTG Cuts at: 2299 2935 3037 3600 5172 2299 Size: 636 102 563 1572 2984 Fragments arranged by size: 2984 1572 636 563 102 ApaI G_GGCC'C Cuts at: 2170 2170 Size: 5857 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2169 3182 1870 Size: 73 226 1013 4545 Fragments arranged by size: 4545 1013 226 73 BamHI G'GATC_C Cuts at: 2685 3187 3554 2685 Size: 502 367 4988 Fragments arranged by size: 4988 502 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 3056 3088 3094 3178 Size: 325 217 627 241 1028 32 6 84 Cuts at: 3178 4633 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1028 627 325 241 217 84 32 6 BanII G_rGCy'C Cuts at: 1391 2170 2473 3044 3123 3176 1391 Size: 779 303 571 79 53 4072 Fragments arranged by size: 4072 779 571 303 79 53 BbeI G_GCGC'C Cuts at: 2032 2032 Size: 5857 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 2849 928 Size: 971 804 146 3936 Fragments arranged by size: 3936 971 804 146 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BfrBI ATG'CAT Cuts at: 2585 2585 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 136 Size: 107 122 71 631 605 2796 1525 Fragments arranged by size: 2796 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3122 3274 3122 Size: 152 5705 Bme1580I G_kGCm'C Cuts at: 1790 2170 3918 5074 1790 Size: 380 1748 1156 2573 Fragments arranged by size: 2573 1748 1156 380 BmgBI CAC'GTC Cuts at: 1318 2016 1318 Size: 698 5159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 3588 4546 Size: 416 53 83 399 24 1117 1054 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1117 1054 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4517 1239 Size: 3278 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2908 4520 882 Size: 617 1409 1612 2219 Fragments arranged by size: 2219 1612 1409 617 BsaAI yAC'GTr Cuts at: 493 1204 2197 3648 493 Size: 711 993 1451 2702 Fragments arranged by size: 2702 1451 993 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 2250 3664 4046 Size: 53 83 186 936 496 221 1414 382 Cuts at: 4046 4681 275 Size: 635 1451 Fragments arranged by size: 1451 1414 936 635 496 382 221 186 83 53 BsaWI w'CCGG_w Cuts at: 3091 4290 4688 4962 5109 5520 3091 Size: 1199 398 274 147 411 3428 Fragments arranged by size: 3428 1199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 2763 2793 1178 Size: 30 165 30 1302 30 28 30 4242 Fragments arranged by size: 4242 1302 165 30 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2321 2905 2908 1186 Size: 1135 584 3 4135 Fragments arranged by size: 4135 1135 584 3 BseYI C'CCAG_C Cuts at: 2798 2976 5060 5556 2798 Size: 178 2084 496 3099 Fragments arranged by size: 3099 2084 496 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2597 3049 1163 Size: 573 861 452 3971 Fragments arranged by size: 3971 861 573 452 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2259 3307 4068 5096 1540 Size: 275 186 258 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 275 258 186 BsiHKAI G_wGCw'C Cuts at: 2082 3123 3918 4003 5074 2082 Size: 1041 795 85 1071 2865 Fragments arranged by size: 2865 1071 1041 795 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2170 2473 3044 3123 3176 3918 Size: 399 292 88 303 571 79 53 742 Cuts at: 3918 4003 5074 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 399 303 292 88 85 79 53 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2619 3212 844 Size: 1775 593 3489 Fragments arranged by size: 3489 1775 593 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2357 3091 4501 4688 1700 Size: 110 278 269 734 1410 187 2869 Fragments arranged by size: 2869 1410 734 278 269 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 2066 2189 2216 2218 2220 1925 Size: 141 123 27 2 2 5562 Fragments arranged by size: 5562 141 123 27 2 2 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2299 794 Size: 1505 4352 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstXI CCAn_nnnn'nTGG Cuts at: 2827 2827 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2685 2802 2952 3187 3554 3939 3956 5408 Size: 713 117 150 235 367 385 17 1452 Cuts at: 5408 5494 5506 5852 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 235 150 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2259 2363 513 Size: 552 1194 104 4007 Fragments arranged by size: 4007 1194 552 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 3144 3304 Size: 54 1425 50 275 18 426 888 160 Cuts at: 3304 4193 5822 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 888 426 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2256 3304 1537 Size: 275 444 1048 4090 Fragments arranged by size: 4090 1048 444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3016 3787 1353 Size: 1663 771 3423 Fragments arranged by size: 3423 1663 771 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2846 4444 4818 4964 387 Size: 1064 138 1257 1598 374 146 1280 Fragments arranged by size: 1598 1280 1257 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3121 3121 Size: 5857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2882 3920 4517 5304 5572 1239 Size: 393 1250 1038 597 787 268 1524 Fragments arranged by size: 1524 1250 1038 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2234 2864 2234 Size: 630 5227 EcoO109I rG'GnC_Cy Cuts at: 2779 2779 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2887 2887 Size: 5857 FspI TGC'GCA Cuts at: 2039 4363 2039 Size: 2324 3533 HaeII r_GCGC'y Cuts at: 1678 2032 2316 5004 1678 Size: 354 284 2688 2531 Fragments arranged by size: 2688 2531 354 284 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2460 2492 2705 2737 2763 Size: 32 135 32 888 32 213 32 26 Cuts at: 2763 2795 4498 4530 4572 4604 1373 Size: 32 1703 32 42 32 2626 Fragments arranged by size: 2626 1703 888 213 135 42 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 2140 3201 3447 669 Size: 988 281 202 1061 246 3079 Fragments arranged by size: 3079 1061 988 281 246 202 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KasI G'GCGC_C Cuts at: 2028 2028 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4035 Fragments arranged by size: 4035 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2590 2825 3815 4174 4333 518 Size: 648 1424 235 990 359 159 2042 Fragments arranged by size: 2042 1424 990 648 359 235 159 MspA1I CmG'CkG Cuts at: 1606 2035 2365 3273 3950 5098 5343 1606 Size: 429 330 908 677 1148 245 2120 Fragments arranged by size: 2120 1148 908 677 429 330 245 NaeI GCC'GGC Cuts at: 2090 2359 2090 Size: 269 5588 NarI GG'CG_CC Cuts at: 2029 2029 Size: 5857 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5305 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NgoMIV G'CCGG_C Cuts at: 2088 2357 2088 Size: 269 5588 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NsiI A_TGCA'T Cuts at: 2587 2587 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3052 3215 4760 1132 Size: 97 1823 163 1545 2229 Fragments arranged by size: 2229 1823 1545 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2779 2779 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2166 2166 Size: 5857 PstI C_TGCA'G Cuts at: 830 2235 2940 3209 830 Size: 1405 705 269 3478 Fragments arranged by size: 3478 1405 705 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2035 2035 Size: 5857 SacI G_AGCT'C Cuts at: 3123 3123 Size: 5857 SacII CC_GC'GG Cuts at: 2366 2366 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SapI GCTCTTCn'nnn_ Cuts at: 3016 3016 Size: 5857 SbfI CC_TGCA'GG Cuts at: 2235 3209 2235 Size: 974 4883 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2231 2512 2936 3205 4340 5021 5212 Size: 219 1186 281 424 269 1135 681 191 Cuts at: 5212 826 Size: 1471 Fragments arranged by size: 1471 1186 1135 681 424 281 269 219 191 SfoI GGC'GCC Cuts at: 2030 2030 Size: 5857 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 3091 1700 Size: 1391 4466 SmaI CCC'GGG Cuts at: 1872 2171 3184 1872 Size: 299 1013 4545 Fragments arranged by size: 4545 1013 299 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 2657 2870 1517 Size: 1140 213 4504 Fragments arranged by size: 4504 1140 213 StyI C'CwwG_G Cuts at: 513 1065 2566 2992 3296 513 Size: 552 1501 426 304 3074 Fragments arranged by size: 3074 1501 552 426 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2830 3884 3901 4054 4239 4684 325 Size: 1255 1250 1054 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1250 1054 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4103 5815 96 Size: 274 80 33 51 1443 2126 1712 138 Fragments arranged by size: 2126 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2481 2513 5817 5849 2481 Size: 32 3304 32 2489 Fragments arranged by size: 3304 2489 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2756 1316 Size: 1440 4417 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2795 2824 2795 Size: 29 5828 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2169 3182 1870 Size: 299 1013 4545 Fragments arranged by size: 4545 1013 299 XmnI GAAnn'nnTTC Cuts at: 2550 3986 2550 Size: 1436 4421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI