Restriction map for
pFN21AE3504
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvaI AviII AvrII BclI BfrI
BlnI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstPI Bsu36I
Cfr10I CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MluI MroI
NaeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 2387 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2132 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2387 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 124 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 840 GGATCC G^GATCC
BanII 910 GGGCTC G[GA]GC[CT]^C
BbrPI 540 CACGTG CAC^GTG
BbvCI 933 GCTGAGG CCTCAGC(-5/-2)
BcgI 101 CGATCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2579 GGATAC GTATCC(6/5)
BglII 354 AGATCT A^GATCT
BlpI 2098 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2098 GCTGAGC GC^T[ACGT]AGC
BsaWI 1206 TCCGGT [AT]^CCGG[AT]
BsgI 1747 GTGCAG GTGCAG(16/14)
BsmFI 898 GGGAC GGGAC(10/14)
BspDI 1957 ATCGAT AT^CGAT
BspMI 644 ACCTGC ACCTGC(4/8)
Bst1107I 262 GTATAC GTA^TAC
BstBI 242 TTCGAA TT^CGAA
BstZ17I 262 GTATAC GTA^TAC
CelII 2098 GCTGAGC GC^T[ACGT]AGC
ClaI 1957 ATCGAT AT^CGAT
DraI 375 TTTAAA TTT^AAA
DraIII 1360 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2387 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2083 GAAGAG CTCTTC(1/4)
EcoRV 1828 GATATC GAT^ATC
HgaI 307 GCGTC GACGC(5/10)
Ksp632I 2083 GAAGAG CTCTTC(1/4)
NspV 242 TTCGAA TT^CGAA
PflFI 124 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 985 CCAATGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 540 CACGTG CAC^GTG
PmlI 540 CACGTG CAC^GTG
PstI 59 CTGCAG CTGCA^G
SapI 2083 GAAGAGC GCTCTTC(1/4)
SexAI 1361 ACCTGGT A^CC[AT]GGT
SfcI 59 CTGCAG C^T[GA][CT]AG
SfuI 242 TTCGAA TT^CGAA
SwaI 374 ATTTAAAT ATTT^AAAT
Tsp45I 2438 GTCAC ^GT[GC]AC
Tth111I 124 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 985 CCAATGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 357 TCTAGA T^CTAGA
XcmI 588 CCAGCTGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 130 GTCTAC GT^[AC][GT]AC
262 GTATAC
AccII 106 CGCG CG^CG
1277 CGCG
Asp700I 1435 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1870 GAAGAACTTC
AspHI 1246 GAGCAC G[AT]GC[AT]^C
2276 GAGCAC
BalI 851 TGGCCA TGG^CCA
1508 TGGCCA
BanI 13 GGCGCC G^G[CT][GA]CC
2044 GGCGCC
BbeI 13 GGCGCC GGCGC^C
2044 GGCGCC
BbsI 1583 GAAGAC GAAGAC(2/6)
2314 GAAGAC
BglI 1510 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2047 GCCGAGGTGGC
BpuAI 1583 GAAGAC GAAGAC(2/6)
2314 GAAGAC
BsaAI 540 CACGTG [CT]AC^GT[GA]
1604 TACGTG
BsiHKAI 1246 GAGCAC G[AT]GC[AT]^C
2276 GAGCAC
BsmAI 65 GAGAC GTCTC(1/5)
749 GAGAC
BsmI 1439 GCATTC GAATGC(1/-1)
2428 GCATTC
BstUI 106 CGCG CG^CG
1277 CGCG
BstXI 535 CCAGACACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
616 CCAAGTGGTTGG
DrdI 595 GACAATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
676 GACGGCTCTGTC
DsaI 1511 CCATGG C^C[GA][CT]GG
2213 CCATGG
EcoT14I 1511 CCATGG C^C[AT][AT]GG
2213 CCATGG
EcoT22I 192 ATGCAT ATGCA^T
892 ATGCAT
FauI 1998 CCCGC CCCGC(4/6)
1699 GCGGG
KasI 13 GGCGCC G^GCGCC
2044 GGCGCC
MaeII 541 ACGT A^CGT
1605 ACGT
MfeI 482 CAATTG C^AATTG
1936 CAATTG
MluNI 851 TGGCCA TGG^CCA
1508 TGGCCA
MscI 851 TGGCCA TGG^CCA
1508 TGGCCA
MseI 376 TTAA T^TAA
1286 TTAA
MunI 482 CAATTG C^AATTG
1936 CAATTG
MvnI 106 CGCG CG^CG
1277 CGCG
NarI 13 GGCGCC GG^CGCC
2044 GGCGCC
NcoI 1511 CCATGG C^CATGG
2213 CCATGG
NdeI 889 CATATG CA^TATG
2414 CATATG
NsiI 192 ATGCAT ATGCA^T
892 ATGCAT
PpuMI 530 AGGACCC [GA]G^G[AT]CC[CT]
2385 AGGACCT
SfoI 13 GGCGCC GGC^GCC
2044 GGCGCC
SphI 26 GCATGC GCATG^C
729 GCATGC
SspI 454 AATATT AAT^ATT
1110 AATATT
StuI 1289 AGGCCT AGG^CCT
1637 AGGCCT
StyI 1511 CCATGG C^C[AT][AT]GG
2213 CCATGG
TaqI 243 TCGA T^CGA
1958 TCGA
Tru9I 376 TTAA T^TAA
1286 TTAA
TthHB8I 243 TCGA T^CGA
1958 TCGA
XmnI 1435 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1870 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AvaII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI Bsp1286I
BspLU11I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp509I TspRI XhoII