(Circular) (Six-base) MAPSORT of: pFN21AE3504.seq Check: 1365 from: 1 to: 7294 pFN21AE3504 7294 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5104 278 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2136 2268 4637 1937 Size: 199 132 2369 4594 Fragments arranged by size: 4594 2369 199 132 Acc65I G'GTAC_C Cuts at: 4615 4615 Size: 7294 AclI AA'CG_TT Cuts at: 1563 5421 5794 1563 Size: 3858 373 3063 Fragments arranged by size: 3858 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 3005 3038 3052 3623 5357 6741 7009 Size: 567 806 33 14 571 1734 1384 268 Cuts at: 7009 1632 Size: 1917 Fragments arranged by size: 1917 1734 1384 806 571 567 268 33 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7097 AflIII A'CryG_T Cuts at: 1203 2230 2287 2544 4566 6193 7170 1203 Size: 1027 57 257 2022 1627 977 1327 Fragments arranged by size: 2022 1627 1327 1027 977 257 57 AgeI A'CCGG_T Cuts at: 6125 6125 Size: 7294 AhdI GACnn_n'nnGTC Cuts at: 4397 6023 4397 Size: 1626 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3360 1184 Size: 2176 5118 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2779 2811 2779 Size: 32 7262 AlwNI CAG_nnn'CTG Cuts at: 4142 5037 6609 4142 Size: 895 1572 4827 Fragments arranged by size: 4827 1572 895 ApaLI G'TGCA_C Cuts at: 5351 6507 5351 Size: 1156 6138 ApoI r'AATT_y Cuts at: 1493 2341 3080 3320 3353 3797 4603 4829 4981 Size: 848 739 240 33 444 806 226 152 Cuts at: 4981 7090 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 848 806 739 444 240 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 5848 160 Size: 872 4816 1606 Fragments arranged by size: 4816 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 AvaI C'yCGr_G Cuts at: 1870 1943 4619 1870 Size: 73 2676 4545 Fragments arranged by size: 4545 2676 73 BamHI G'GATC_C Cuts at: 2845 4624 4991 2845 Size: 1779 367 5148 Fragments arranged by size: 5148 1779 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 4049 4615 6070 618 Size: 325 217 627 231 2031 566 1455 1842 Fragments arranged by size: 2031 1842 1455 627 566 325 231 217 BanII G_rGCy'C Cuts at: 1391 2919 4613 1391 Size: 1528 1694 4072 Fragments arranged by size: 4072 1694 1528 BbeI G_GCGC'C Cuts at: 2022 4053 2022 Size: 2031 5263 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3595 4326 928 Size: 971 1696 731 3896 Fragments arranged by size: 3896 1696 971 731 BbvCI CC'TCA_GC Cuts at: 2939 2939 Size: 7294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2095 2129 5485 5519 1903 Size: 34 50 34 74 34 3356 34 3678 Fragments arranged by size: 3678 3356 74 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4578 5186 6396 4578 Size: 608 1210 5476 Fragments arranged by size: 5476 1210 608 BclI T'GATC_A Cuts at: 1548 1548 Size: 7294 BfrBI ATG'CAT Cuts at: 2199 2899 2199 Size: 700 6594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3521 4058 5905 Size: 107 122 71 631 605 1849 537 1847 Cuts at: 5905 136 Size: 1525 Fragments arranged by size: 1849 1847 1525 631 605 537 122 107 71 BglII A'GATC_T Cuts at: 2359 7289 2359 Size: 4930 2364 BlpI GC'TnA_GC Cuts at: 4104 4711 4104 Size: 607 6687 Bme1580I G_kGCm'C Cuts at: 1790 2094 2118 2978 3688 5355 6511 1790 Size: 304 24 860 710 1667 1156 2573 Fragments arranged by size: 2573 1667 1156 860 710 304 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7294 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2792 3793 5025 Size: 416 53 83 399 24 1375 1001 1232 Cuts at: 5025 5983 442 Size: 958 1753 Fragments arranged by size: 1753 1375 1232 1001 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7294 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 2678 Size: 7294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2762 2903 3697 4167 4173 5954 1239 Size: 1523 141 794 470 6 1781 2579 Fragments arranged by size: 2579 1781 1523 794 470 141 6 Bpu10I CC'TnA_GC Cuts at: 2939 2939 Size: 7294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5419 6284 6582 5419 Size: 865 298 6131 Fragments arranged by size: 6131 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5957 882 Size: 617 4458 2219 Fragments arranged by size: 4458 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2547 3611 5085 493 Size: 711 1343 1064 1474 2702 Fragments arranged by size: 2702 1474 1343 1064 711 BsaBI GATnn'nnATC Cuts at: 7288 7288 Size: 7294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2312 4050 5101 Size: 53 83 186 936 486 293 1738 1051 Cuts at: 5101 5483 6118 275 Size: 382 635 1451 Fragments arranged by size: 1738 1451 1051 936 635 486 382 293 186 83 53 BsaWI w'CCGG_w Cuts at: 3211 5727 6125 6399 6546 6957 3211 Size: 2516 398 274 147 411 3548 Fragments arranged by size: 3548 2516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2779 2809 4140 4170 1178 Size: 30 165 30 1376 30 1331 30 4302 Fragments arranged by size: 4302 1376 1331 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 3263 3316 1186 Size: 1294 783 53 5164 Fragments arranged by size: 5164 1294 783 53 BseYI C'CCAG_C Cuts at: 2592 6497 6993 2592 Size: 3905 496 2893 Fragments arranged by size: 3905 2893 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3773 1163 Size: 573 2037 4684 Fragments arranged by size: 4684 2037 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4744 5505 6533 1540 Size: 275 186 2743 761 1028 2301 Fragments arranged by size: 2743 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3255 4285 5355 5440 6511 3255 Size: 1030 1070 85 1071 4038 Fragments arranged by size: 4038 1071 1070 1030 85 BsmI GAATG_Cn' Cuts at: 3444 4433 4805 4898 3444 Size: 989 372 93 5840 Fragments arranged by size: 5840 989 372 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2118 2919 2978 3255 3688 4285 Size: 399 304 24 801 59 277 433 597 Cuts at: 4285 4613 5355 5440 6511 1391 Size: 328 742 85 1071 2174 Fragments arranged by size: 2174 1071 801 742 597 433 399 328 304 277 85 59 24 BspEI T'CCGG_A Cuts at: 6957 6957 Size: 7294 BspHI T'CATG_A Cuts at: 5183 6913 5183 Size: 1730 5564 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2658 4649 844 Size: 1814 1991 3489 Fragments arranged by size: 3489 1991 1814 BsrBI CCG'CTC Cuts at: 1394 1823 5181 1394 Size: 429 3358 3507 Fragments arranged by size: 3507 3358 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5789 5963 65 Size: 1183 4541 174 1396 Fragments arranged by size: 4541 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5938 6125 1700 Size: 110 4128 187 2869 Fragments arranged by size: 4128 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7294 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7294 BssSI C'ACGA_G Cuts at: 5354 6366 5354 Size: 1012 6282 BstAPI GCAn_nnn'nTGC Cuts at: 794 3036 3450 3701 4142 794 Size: 2242 414 251 441 3946 Fragments arranged by size: 3946 2242 441 414 251 BstBI TT'CG_AA Cuts at: 2248 6098 2248 Size: 3850 3444 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7294 BstXI CCAn_nnnn'nTGG Cuts at: 2547 2628 2547 Size: 81 7213 BstYI r'GATC_y Cuts at: 1972 2359 2845 3136 3273 4293 4409 4624 4991 Size: 387 486 291 137 1020 116 215 367 Cuts at: 4991 5376 5393 6845 6931 6943 7289 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1020 486 387 385 367 346 291 215 137 116 86 17 12 BstZ17I GTA'TAC Cuts at: 2269 2269 Size: 7294 BtgI C'CryG_G Cuts at: 513 1065 3516 4218 513 Size: 552 2451 702 3589 Fragments arranged by size: 3589 2451 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3429 487 Size: 683 56 2203 4352 Fragments arranged by size: 4352 2203 683 56 BtsI GCAGTG_nn' Cuts at: 3688 4810 5604 5624 3688 Size: 1122 794 20 5358 Fragments arranged by size: 5358 1122 794 20 ClaI AT'CG_AT Cuts at: 1554 3963 1554 Size: 2409 4885 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4511 4546 529 Size: 35 3947 35 3277 Fragments arranged by size: 3947 3277 35 35 DraI TTT'AAA Cuts at: 2382 4598 2382 Size: 2216 5078 DraIII CAC_nnn'GTG Cuts at: 3370 3370 Size: 7294 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2606 2687 6301 809 Size: 507 1290 81 3614 1802 Fragments arranged by size: 3614 1802 1290 507 81 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2856 3513 3987 Size: 54 1425 50 275 18 1026 657 474 Cuts at: 3987 4741 5630 7259 8 Size: 754 889 1629 43 Fragments arranged by size: 1629 1425 1026 889 754 657 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4741 1537 Size: 275 2929 4090 Fragments arranged by size: 4090 2929 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4083 5224 1353 Size: 2730 1141 3423 Fragments arranged by size: 3423 2730 1141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5881 6255 6401 387 Size: 1064 138 4292 374 146 1280 Fragments arranged by size: 4292 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2762 2903 3005 3038 3052 3623 Size: 393 567 563 141 102 33 14 571 Cuts at: 3623 3697 4167 4173 5357 5954 6741 7009 1239 Size: 74 470 6 1184 597 787 268 1524 Fragments arranged by size: 1524 1184 787 597 571 567 563 470 393 268 141 102 74 33 14 6 EcoNI CCTnn'n_nnAGG Cuts at: 2221 3331 4540 2221 Size: 1110 1209 4975 Fragments arranged by size: 4975 1209 1110 EcoO109I rG'GnC_Cy Cuts at: 2536 4391 4545 2536 Size: 1855 154 5285 Fragments arranged by size: 5285 1855 154 EcoRI G'AATT_C Cuts at: 2341 3080 3320 3353 4603 4981 2341 Size: 739 240 33 1250 378 4654 Fragments arranged by size: 4654 1250 739 378 240 33 EcoRV GAT'ATC Cuts at: 3835 3835 Size: 7294 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2435 2725 2435 Size: 290 7004 FspI TGC'GCA Cuts at: 5800 5800 Size: 7294 HaeII r_GCGC'y Cuts at: 1678 2014 2022 4053 6441 1678 Size: 336 8 2031 2388 2531 Fragments arranged by size: 2531 2388 2031 336 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3956 3988 4383 4415 5935 Size: 32 135 32 2384 32 395 32 1520 Cuts at: 5935 5967 6009 6041 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2384 1520 395 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4638 4884 669 Size: 988 281 2700 246 3079 Fragments arranged by size: 3079 2700 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7294 HpaI GTT'AAC Cuts at: 4884 4884 Size: 7294 KasI G'GCGC_C Cuts at: 2018 4049 2018 Size: 2031 5263 KpnI G_GTAC'C Cuts at: 4619 4619 Size: 7294 MfeI C'AATT_G Cuts at: 2487 3941 4893 2487 Size: 1454 952 4888 Fragments arranged by size: 4888 1454 952 MluI A'CGCG_T Cuts at: 7170 7170 Size: 7294 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2609 3164 6408 6592 1305 Size: 1304 555 3244 184 2007 Fragments arranged by size: 3244 2007 1304 555 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2858 3515 10 Size: 54 1425 343 1026 657 3789 Fragments arranged by size: 3789 1425 1026 657 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2898 3161 4217 5252 5611 5770 518 Size: 648 1732 263 1056 1035 359 159 2042 Fragments arranged by size: 2042 1732 1056 1035 648 359 263 159 MspA1I CmG'CkG Cuts at: 1606 2046 2596 3036 3985 4006 4111 4710 5387 Size: 440 550 440 949 21 105 599 677 Cuts at: 5387 6535 6780 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 949 677 599 550 440 440 245 105 21 NarI GG'CG_CC Cuts at: 2019 4050 2019 Size: 2031 5263 NcoI C'CATG_G Cuts at: 513 1065 3516 4218 513 Size: 552 2451 702 3589 Fragments arranged by size: 3589 2451 702 552 NdeI CA'TA_TG Cuts at: 387 2895 4420 387 Size: 2508 1525 3261 Fragments arranged by size: 3261 2508 1525 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7294 NotI GC'GGCC_GC Cuts at: 4741 4741 Size: 7294 NsiI A_TGCA'T Cuts at: 2201 2901 2201 Size: 700 6594 NspI r_CATG'y Cuts at: 1132 1229 2035 2101 2234 2275 2291 2738 3289 Size: 97 806 66 133 41 16 447 551 Cuts at: 3289 4017 4570 4652 6197 1132 Size: 728 553 82 1545 2229 Fragments arranged by size: 2229 1545 806 728 553 551 447 133 97 82 66 41 16 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7294 PciI A'CATG_T Cuts at: 2230 2287 4566 6193 2230 Size: 57 2279 1627 3331 Fragments arranged by size: 3331 2279 1627 57 PflMI CCAn_nnn'nTGG Cuts at: 1108 2996 1108 Size: 1888 5406 PfoI T'CCnGG_A Cuts at: 2919 2964 2919 Size: 45 7249 PmeI GTTT'AAAC Cuts at: 4598 4598 Size: 7294 PmlI CAC'GTG Cuts at: 2547 2547 Size: 7294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2779 2811 3260 3292 5363 5395 6876 6908 2779 Size: 32 449 32 2071 32 1481 32 3165 Fragments arranged by size: 3165 2071 1481 449 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2536 4391 2536 Size: 1855 5439 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7294 PsiI TTA'TAA Cuts at: 4864 4864 Size: 7294 PstI C_TGCA'G Cuts at: 830 2068 4646 830 Size: 1238 2578 3478 Fragments arranged by size: 3478 2578 1238 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7294 PvuII CAG'CTG Cuts at: 2596 3036 3985 2596 Size: 440 949 5905 Fragments arranged by size: 5905 949 440 SalI G'TCGA_C Cuts at: 1936 4636 1936 Size: 2700 4594 SapI GCTCTTCn'nnn_ Cuts at: 4083 4083 Size: 7294 SbfI CC_TGCA'GG Cuts at: 4646 4646 Size: 7294 ScaI AGT'ACT Cuts at: 5542 7254 5542 Size: 1712 5582 SexAI A'CCwGG_T Cuts at: 3366 3366 Size: 7294 SfcI C'TryA_G Cuts at: 826 1045 2064 4642 5777 6458 6649 826 Size: 219 1019 2578 1135 681 191 1471 Fragments arranged by size: 2578 1471 1135 1019 681 219 191 SfoI GGC'GCC Cuts at: 2020 4051 2020 Size: 2031 5263 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7294 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7294 SmaI CCC'GGG Cuts at: 1872 4621 1872 Size: 2749 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5398 6299 6561 820 Size: 197 926 3455 901 262 1553 Fragments arranged by size: 3455 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5085 493 Size: 4592 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7294 SphI G_CATG'C Cuts at: 1132 2035 2738 4652 1132 Size: 903 703 1914 3774 Fragments arranged by size: 3774 1914 903 703 SspI AAT'ATT Cuts at: 5 52 2461 3117 5218 5 Size: 47 2409 656 2101 2081 Fragments arranged by size: 2409 2101 2081 656 47 StuI AGG'CCT Cuts at: 1517 3296 3644 1517 Size: 1779 348 5167 Fragments arranged by size: 5167 1779 348 StyI C'CwwG_G Cuts at: 513 1065 3516 4218 4733 513 Size: 552 2451 702 515 3074 Fragments arranged by size: 3074 2451 702 552 515 SwaI ATTT'AAAT Cuts at: 2382 2382 Size: 7294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2606 4495 5321 5338 5491 5676 6121 Size: 1255 1026 1889 826 17 153 185 445 Cuts at: 6121 325 Size: 1498 Fragments arranged by size: 1889 1498 1255 1026 826 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4029 4569 5540 Size: 274 80 33 51 1443 2052 540 971 Cuts at: 5540 7252 96 Size: 1712 138 Fragments arranged by size: 2052 1712 1443 971 540 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4491 4523 7254 7286 4491 Size: 32 2731 32 4499 Fragments arranged by size: 4499 2731 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2132 1316 Size: 816 6478 XbaI T'CTAG_A Cuts at: 2362 4630 2362 Size: 2268 5026 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2600 2600 Size: 7294 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7294 XmaI C'CCGG_G Cuts at: 1870 4619 1870 Size: 2749 4545 XmnI GAAnn'nnTTC Cuts at: 3444 3879 5423 3444 Size: 435 1544 5315 Fragments arranged by size: 5315 1544 435 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5102 276 Size: 53 83 186 936 3568 2468 Fragments arranged by size: 3568 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BsiWI BsmBI Bsu36I EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI