Restriction map for
pFN21AE3504
(Linear) (Six-base) MAPSORT of: KIEE3504.seq Check: 1714 from: 1 to: 2589
KIEE3504.orf
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 0 131 263 2589
Size: 131 132 2326
Fragments arranged by size:
2326 132 131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1000 1033 1047 1618 2589
Size: 194 806 33 14 571 971
Fragments arranged by size:
971 806 571 194 33 14
AflIII A'CryG_T
Cuts at: 0 225 282 539 2561 2589
Size: 225 57 257 2022 28
Fragments arranged by size:
2022 257 225 57 28
AhdI GACnn_n'nnGTC
Cuts at: 0 2392 2589
Size: 2392 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1355 2589
Size: 1355 1234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 774 806 2589
Size: 774 32 1783
Fragments arranged by size:
1783 774 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2137 2589
Size: 2137 452
ApoI r'AATT_y
Cuts at: 0 336 1075 1315 1348 1792 2589
Size: 336 739 240 33 444 797
Fragments arranged by size:
797 739 444 336 240 33
BamHI G'GATC_C
Cuts at: 0 840 2589
Size: 840 1749
BanI G'GyrC_C
Cuts at: 0 13 2044 2589
Size: 13 2031 545
Fragments arranged by size:
2031 545 13
BanII G_rGCy'C
Cuts at: 0 914 2589
Size: 914 1675
BbeI G_GCGC'C
Cuts at: 0 17 2048 2589
Size: 17 2031 541
Fragments arranged by size:
2031 541 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1590 2321 2589
Size: 1590 731 268
Fragments arranged by size:
1590 731 268
BbvCI CC'TCA_GC
Cuts at: 0 934 2589
Size: 934 1655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 90 124 2589
Size: 90 34 2465
Fragments arranged by size:
2465 90 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2573 2589
Size: 2573 16
BfrBI ATG'CAT
Cuts at: 0 194 894 2589
Size: 194 700 1695
Fragments arranged by size:
1695 700 194
BglI GCCn_nnn'nGGC
Cuts at: 0 1516 2053 2589
Size: 1516 537 536
Fragments arranged by size:
1516 537 536
BglII A'GATC_T
Cuts at: 0 354 2589
Size: 354 2235
BlpI GC'TnA_GC
Cuts at: 0 2099 2589
Size: 2099 490
Bme1580I G_kGCm'C
Cuts at: 0 89 113 973 1683 2589
Size: 89 24 860 710 906
Fragments arranged by size:
906 860 710 89 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 787 1788 2589
Size: 787 1001 801
Fragments arranged by size:
1001 801 787
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 673 2589
Size: 673 1916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 898 1692 2162 2168 2589
Size: 757 141 794 470 6 421
Fragments arranged by size:
794 757 470 421 141 6
Bpu10I CC'TnA_GC
Cuts at: 0 934 2589
Size: 934 1655
BsaAI yAC'GTr
Cuts at: 0 542 1606 2589
Size: 542 1064 983
Fragments arranged by size:
1064 983 542
BsaHI Gr'CG_yC
Cuts at: 0 14 307 2045 2589
Size: 14 293 1738 544
Fragments arranged by size:
1738 544 293 14
BsaWI w'CCGG_w
Cuts at: 0 1206 2589
Size: 1206 1383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 774 804 2135 2165 2589
Size: 774 30 1331 30 424
Fragments arranged by size:
1331 774 424 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 1258 1311 2589
Size: 475 783 53 1278
Fragments arranged by size:
1278 783 475 53
BseYI C'CCAG_C
Cuts at: 0 587 2589
Size: 587 2002
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 2589
Size: 1768 821
BsiHKAI G_wGCw'C
Cuts at: 0 1250 2280 2589
Size: 1250 1030 309
Fragments arranged by size:
1250 1030 309
BsmI GAATG_Cn'
Cuts at: 0 1439 2428 2589
Size: 1439 989 161
Fragments arranged by size:
1439 989 161
Bsp1286I G_dGCh'C
Cuts at: 0 89 113 914 973 1250 1683 2280 2589
Size: 89 24 801 59 277 433 597 309
Fragments arranged by size:
801 597 433 309 277 89 59 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 653 2589
Size: 653 1936
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1031 1445 1696 2137 2589
Size: 1031 414 251 441 452
Fragments arranged by size:
1031 452 441 414 251
BstBI TT'CG_AA
Cuts at: 0 243 2589
Size: 243 2346
BstXI CCAn_nnnn'nTGG
Cuts at: 0 542 623 2589
Size: 542 81 1966
Fragments arranged by size:
1966 542 81
BstYI r'GATC_y
Cuts at: 0 354 840 1131 1268 2288 2404 2589
Size: 354 486 291 137 1020 116 185
Fragments arranged by size:
1020 486 354 291 185 137 116
BstZ17I GTA'TAC
Cuts at: 0 264 2589
Size: 264 2325
BtgI C'CryG_G
Cuts at: 0 1511 2213 2589
Size: 1511 702 376
Fragments arranged by size:
1511 702 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1424 2589
Size: 1424 1165
BtsI GCAGTG_nn'
Cuts at: 0 1683 2589
Size: 1683 906
ClaI AT'CG_AT
Cuts at: 0 1958 2589
Size: 1958 631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2506 2541 2589
Size: 2506 35 48
Fragments arranged by size:
2506 48 35
DraI TTT'AAA
Cuts at: 0 377 2589
Size: 377 2212
DraIII CAC_nnn'GTG
Cuts at: 0 1365 2589
Size: 1365 1224
DrdI GACnn_nn'nnGTC
Cuts at: 0 601 682 2589
Size: 601 81 1907
Fragments arranged by size:
1907 601 81
EaeI y'GGCC_r
Cuts at: 0 851 1508 1982 2589
Size: 851 657 474 607
Fragments arranged by size:
851 657 607 474
EarI CTCTTCn'nnn_
Cuts at: 0 2078 2589
Size: 2078 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 757 898 1000 1033 1047 1618 1692
Size: 194 563 141 102 33 14 571 74
Cuts at: 1692 2162 2168 2589
Size: 470 6 421
Fragments arranged by size:
571 563 470 421 194 141 102 74
33 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 216 1326 2535 2589
Size: 216 1110 1209 54
Fragments arranged by size:
1209 1110 216 54
EcoO109I rG'GnC_Cy
Cuts at: 0 531 2386 2540 2589
Size: 531 1855 154 49
Fragments arranged by size:
1855 531 154 49
EcoRI G'AATT_C
Cuts at: 0 336 1075 1315 1348 2589
Size: 336 739 240 33 1241
Fragments arranged by size:
1241 739 336 240 33
EcoRV GAT'ATC
Cuts at: 0 1830 2589
Size: 1830 759
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 430 720 2589
Size: 430 290 1869
Fragments arranged by size:
1869 430 290
HaeII r_GCGC'y
Cuts at: 0 9 17 2048 2589
Size: 9 8 2031 541
Fragments arranged by size:
2031 541 9 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1951 1983 2378 2410 2589
Size: 1951 32 395 32 179
Fragments arranged by size:
1951 395 179 32 32
KasI G'GCGC_C
Cuts at: 0 13 2044 2589
Size: 13 2031 545
Fragments arranged by size:
2031 545 13
MfeI C'AATT_G
Cuts at: 0 482 1936 2589
Size: 482 1454 653
Fragments arranged by size:
1454 653 482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1159 2589
Size: 604 555 1430
Fragments arranged by size:
1430 604 555
MscI TGG'CCA
Cuts at: 0 853 1510 2589
Size: 853 657 1079
Fragments arranged by size:
1079 853 657
MslI CAynn'nnrTG
Cuts at: 0 893 1156 2212 2589
Size: 893 263 1056 377
Fragments arranged by size:
1056 893 377 263
MspA1I CmG'CkG
Cuts at: 0 41 591 1031 1980 2001 2106 2589
Size: 41 550 440 949 21 105 483
Fragments arranged by size:
949 550 483 440 105 41 21
NarI GG'CG_CC
Cuts at: 0 14 2045 2589
Size: 14 2031 544
Fragments arranged by size:
2031 544 14
NcoI C'CATG_G
Cuts at: 0 1511 2213 2589
Size: 1511 702 376
Fragments arranged by size:
1511 702 376
NdeI CA'TA_TG
Cuts at: 0 890 2415 2589
Size: 890 1525 174
Fragments arranged by size:
1525 890 174
NsiI A_TGCA'T
Cuts at: 0 196 896 2589
Size: 196 700 1693
Fragments arranged by size:
1693 700 196
NspI r_CATG'y
Cuts at: 0 30 96 229 270 286 733 1284 2012
Size: 30 66 133 41 16 447 551 728
Cuts at: 2012 2565 2589
Size: 553 24
Fragments arranged by size:
728 553 551 447 133 66 41 30
24 16
PciI A'CATG_T
Cuts at: 0 225 282 2561 2589
Size: 225 57 2279 28
Fragments arranged by size:
2279 225 57 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 991 2589
Size: 991 1598
PfoI T'CCnGG_A
Cuts at: 0 914 959 2589
Size: 914 45 1630
Fragments arranged by size:
1630 914 45
PmlI CAC'GTG
Cuts at: 0 542 2589
Size: 542 2047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 774 806 1255 1287 2589
Size: 774 32 449 32 1302
Fragments arranged by size:
1302 774 449 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 531 2386 2589
Size: 531 1855 203
Fragments arranged by size:
1855 531 203
PstI C_TGCA'G
Cuts at: 0 63 2589
Size: 63 2526
PvuII CAG'CTG
Cuts at: 0 591 1031 1980 2589
Size: 591 440 949 609
Fragments arranged by size:
949 609 591 440
SapI GCTCTTCn'nnn_
Cuts at: 0 2078 2589
Size: 2078 511
SexAI A'CCwGG_T
Cuts at: 0 1361 2589
Size: 1361 1228
SfcI C'TryA_G
Cuts at: 0 59 2589
Size: 59 2530
SfoI GGC'GCC
Cuts at: 0 15 2046 2589
Size: 15 2031 543
Fragments arranged by size:
2031 543 15
SphI G_CATG'C
Cuts at: 0 30 733 2589
Size: 30 703 1856
Fragments arranged by size:
1856 703 30
SspI AAT'ATT
Cuts at: 0 456 1112 2589
Size: 456 656 1477
Fragments arranged by size:
1477 656 456
StuI AGG'CCT
Cuts at: 0 1291 1639 2589
Size: 1291 348 950
Fragments arranged by size:
1291 950 348
StyI C'CwwG_G
Cuts at: 0 1511 2213 2589
Size: 1511 702 376
Fragments arranged by size:
1511 702 376
SwaI ATTT'AAAT
Cuts at: 0 377 2589
Size: 377 2212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 601 2490 2589
Size: 601 1889 99
Fragments arranged by size:
1889 601 99
TatI w'GTAC_w
Cuts at: 0 2024 2564 2589
Size: 2024 540 25
Fragments arranged by size:
2024 540 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2486 2518 2589
Size: 2486 32 71
Fragments arranged by size:
2486 71 32
Tth111I GACn'n_nGTC
Cuts at: 0 127 2589
Size: 127 2462
XbaI T'CTAG_A
Cuts at: 0 357 2589
Size: 357 2232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 595 2589
Size: 595 1994
XmnI GAAnn'nnTTC
Cuts at: 0 1439 1874 2589
Size: 1439 435 715
Fragments arranged by size:
1439 715 435
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AloI AlwNI ApoI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NsiI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PstI PvuII SapI SexAI
SfcI SfoI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BclI BmgBI BmtI BpuEI BsaI BsaBI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstEII Bsu36I EagI EciI EcoICRI FseI FspI
FspAI HincII HindIII HpaI KpnI MluI NaeI NgoMIV
NheI NotI NruI PacI PasI PmeI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI XhoI XmaI ZraI