(Circular) (Six-base) MAPSORT of: pFN21AA1150.seq Check: 6134 from: 1 to: 6484 pFN21AA1150 6484 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4547 5931 6199 1632 Size: 2915 1384 268 1917 Fragments arranged by size: 2915 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2778 2794 5383 6360 1203 Size: 1575 16 2589 977 1327 Fragments arranged by size: 2589 1575 1327 977 16 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6484 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2984 3016 2984 Size: 32 6452 AlwNI CAG_nnn'CTG Cuts at: 4227 5799 4227 Size: 1572 4912 ApaI G_GGCC'C Cuts at: 2698 2840 2698 Size: 142 6342 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 2716 3793 4019 4171 6280 1493 Size: 1223 1077 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 1077 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3809 1870 Size: 73 1866 4545 Fragments arranged by size: 4545 1866 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 3610 3805 5260 618 Size: 325 217 627 392 1431 195 1455 1842 Fragments arranged by size: 1842 1455 1431 627 392 325 217 195 BanII G_rGCy'C Cuts at: 1391 2698 2840 3803 1391 Size: 1307 142 963 4072 Fragments arranged by size: 4072 1307 963 142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2606 928 Size: 971 707 4806 Fragments arranged by size: 4806 971 707 BbvCI CC'TCA_GC Cuts at: 2510 2510 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BfrBI ATG'CAT Cuts at: 3395 3395 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3065 5095 136 Size: 107 122 71 631 605 1393 2030 1525 Fragments arranged by size: 2030 1525 1393 631 605 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2698 2840 3268 4545 5701 1790 Size: 908 142 428 1277 1156 2573 Fragments arranged by size: 2573 1277 1156 908 428 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3262 3610 3696 Size: 416 53 83 399 24 1845 348 86 Cuts at: 3696 4215 5173 442 Size: 519 958 1753 Fragments arranged by size: 1845 1753 958 519 416 399 348 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2589 3119 3459 3542 5144 1239 Size: 1350 530 340 83 1602 2579 Fragments arranged by size: 2579 1602 1350 530 340 83 Bpu10I CC'TnA_GC Cuts at: 2510 2844 2510 Size: 334 6150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 4609 5474 5772 2195 Size: 2414 865 298 2907 Fragments arranged by size: 2907 2414 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3497 5147 882 Size: 617 1998 1650 2219 Fragments arranged by size: 2219 1998 1650 617 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4291 4673 5308 275 Size: 53 83 186 936 2758 382 635 1451 Fragments arranged by size: 2758 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3457 3487 1178 Size: 30 165 30 2054 30 4175 Fragments arranged by size: 4175 2054 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 1186 Size: 1312 5172 BseYI C'CCAG_C Cuts at: 2062 2812 2817 3020 5687 6183 2062 Size: 750 5 203 2667 496 2363 Fragments arranged by size: 2667 2363 750 496 203 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3171 1163 Size: 573 1435 4476 Fragments arranged by size: 4476 1435 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3934 4695 5723 1540 Size: 275 186 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4545 4630 5701 4545 Size: 85 1071 5328 Fragments arranged by size: 5328 1071 85 BsmI GAATG_Cn' Cuts at: 2623 3995 4088 2623 Size: 1372 93 5019 Fragments arranged by size: 5019 1372 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2698 2840 3268 3803 4545 4630 5701 Size: 399 908 142 428 535 742 85 1071 Cuts at: 5701 1391 Size: 2174 Fragments arranged by size: 2174 1071 908 742 535 428 399 142 85 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 3510 4373 6103 3510 Size: 863 1730 3891 Fragments arranged by size: 3891 1730 863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3839 844 Size: 2995 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2488 3585 4371 1394 Size: 429 665 1097 786 3507 Fragments arranged by size: 3507 1097 786 665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2144 3660 4979 5153 65 Size: 1183 896 1516 1319 174 1396 Fragments arranged by size: 1516 1396 1319 1183 896 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3381 5128 5315 1700 Size: 110 1571 1747 187 2869 Fragments arranged by size: 2869 1747 1571 187 110 BsrGI T'GTAC_A Cuts at: 96 2611 96 Size: 2515 3969 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstYI r'GATC_y Cuts at: 1972 3102 3814 4181 4566 4583 6035 6121 6133 Size: 1130 712 367 385 17 1452 86 12 Cuts at: 6133 6479 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1130 712 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 513 Size: 552 939 117 4876 Fragments arranged by size: 4876 939 552 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 2218 3338 4000 4794 4814 2218 Size: 1120 662 794 20 3888 Fragments arranged by size: 3888 1120 794 662 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2763 2798 529 Size: 35 2199 35 4215 Fragments arranged by size: 4215 2199 35 35 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3931 4820 6449 Size: 54 1425 50 275 18 2101 889 1629 Cuts at: 6449 8 Size: 43 Fragments arranged by size: 2101 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 3075 4414 1353 Size: 681 1041 1339 3423 Fragments arranged by size: 3423 1339 1041 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5071 5445 5591 387 Size: 1064 138 3482 374 146 1280 Fragments arranged by size: 3482 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2589 3119 3459 3542 4547 5144 5931 Size: 393 957 530 340 83 1005 597 787 Cuts at: 5931 6199 1239 Size: 268 1524 Fragments arranged by size: 1524 1005 957 787 597 530 393 340 268 83 EcoNI CCTnn'n_nnAGG Cuts at: 3413 3413 Size: 6484 EcoO109I rG'GnC_Cy Cuts at: 2665 2694 2836 3698 2665 Size: 29 142 862 5451 Fragments arranged by size: 5451 862 142 29 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2045 2071 2255 2045 Size: 26 184 6274 Fragments arranged by size: 6274 184 26 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 3027 5631 1678 Size: 1349 2604 2531 Fragments arranged by size: 2604 2531 1349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2554 2586 5125 Size: 32 135 32 495 32 455 32 2539 Cuts at: 5125 5157 5199 5231 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2539 495 455 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3658 3828 4074 669 Size: 988 281 1720 170 246 3079 Fragments arranged by size: 3079 1720 988 281 246 170 HindIII A'AGCT_T Cuts at: 748 2675 748 Size: 1927 4557 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3369 5598 5782 1305 Size: 2064 2229 184 2007 Fragments arranged by size: 2229 2064 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4662 Fragments arranged by size: 4662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2783 2954 3173 4442 4801 4960 518 Size: 648 1617 171 219 1269 359 159 2042 Fragments arranged by size: 2042 1617 1269 648 359 219 171 159 MspA1I CmG'CkG Cuts at: 1606 2509 2833 3082 3479 3900 4577 5725 5970 Size: 903 324 249 397 421 677 1148 245 Cuts at: 5970 1606 Size: 2120 Fragments arranged by size: 2120 1148 903 677 421 397 324 249 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2121 513 Size: 552 939 117 4876 Fragments arranged by size: 4876 939 552 117 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NsiI A_TGCA'T Cuts at: 3397 3397 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 2782 3842 5387 1132 Size: 97 1553 1060 1545 2229 Fragments arranged by size: 2229 1553 1545 1060 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 2778 5383 2778 Size: 2605 3879 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6484 PfoI T'CCnGG_A Cuts at: 3096 3096 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2694 2836 2694 Size: 142 6342 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3423 3455 3423 Size: 32 6452 PstI C_TGCA'G Cuts at: 830 3058 3836 830 Size: 2228 778 3478 Fragments arranged by size: 3478 2228 778 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2509 3082 3479 2509 Size: 573 397 5514 Fragments arranged by size: 5514 573 397 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SapI GCTCTTCn'nnn_ Cuts at: 2034 3075 2034 Size: 1041 5443 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2576 2822 3054 3410 3832 4967 5648 Size: 219 1531 246 232 356 422 1135 681 Cuts at: 5648 5839 826 Size: 191 1471 Fragments arranged by size: 1531 1471 1135 681 422 356 246 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 4588 5489 5751 820 Size: 197 926 231 2414 901 262 1553 Fragments arranged by size: 2414 1553 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 2257 1517 Size: 740 5744 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2698 3217 3640 3923 513 Size: 552 939 117 577 519 423 283 3074 Fragments arranged by size: 3074 939 577 552 519 423 283 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2887 3729 4511 4528 4681 4866 5311 Size: 1255 1307 842 782 17 153 185 445 Cuts at: 5311 325 Size: 1498 Fragments arranged by size: 1498 1307 1255 842 782 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2611 4730 6442 Size: 274 80 33 51 1443 634 2119 1712 Cuts at: 6442 96 Size: 138 Fragments arranged by size: 2119 1712 1443 634 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3457 3489 6444 6476 3457 Size: 32 2955 32 3465 Fragments arranged by size: 3465 2955 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2568 1316 Size: 1252 5232 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2425 2425 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI