Restriction map for
pFN21AB5337
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiWI BsmBI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HinfI HpaI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tru9I
Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 107 CGCG CG^CG
AcsI 434 AAATTC [GA]^AATT[CT]
AcyI 480 GGCGCC G[GA]^CG[CT]C
AfaI 207 GTAC GT^AC
AlwNI 384 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 43 GGGCCC GGGCC^C
ApoI 434 AAATTC [GA]^AATT[CT]
Asp700I 420 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 447 GAGCAC G[AT]GC[AT]^C
BbeI 480 GGCGCC GGCGC^C
BbsI 693 GAAGAC GAAGAC(2/6)
BfaI 103 CTAG C^TAG
BpuAI 693 GAAGAC GAAGAC(2/6)
BsaHI 480 GGCGCC G[GA]^CG[CT]C
BsaI 440 GAGACC GGTCTC(1/5)
BsiEI 612 CGACCG CG[GA][CT]^CG
BsiHKAI 447 GAGCAC G[AT]GC[AT]^C
BsmAI 440 GAGAC GTCTC(1/5)
Bsp1407I 206 TGTACA T^GTACA
BspMI 407 GCAGGT ACCTGC(4/8)
BsrBI 547 GAGCGG CCGCTC(-3/-3)
BsrGI 206 TGTACA T^GTACA
BsrI 452 CCAGT ACTGG(1/-1)
BssHII 106 GCGCGC G^CGCGC
BstEII 361 GGTGACC G^GT[ACGT]ACC
BstPI 361 GGTGACC G^GT[ACGT]ACC
BstUI 107 CGCG CG^CG
DpnII 315 GATC ^GATC
DsaI 122 CCGTGG C^C[GA][CT]GG
EcoO65I 361 GGTGACC G^GT[ACGT]ACC
Hin1I 480 GGCGCC G[GA]^CG[CT]C
HindIII 706 AAGCTT A^AGCTT
KasI 480 GGCGCC G^GCGCC
MaeI 103 CTAG C^TAG
MboI 315 GATC ^GATC
MboII 693 GAAGA GAAGA(8/7)
MslI 273 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 751 CAGCTG C[AC]G^C[GT]G
MvnI 107 CGCG CG^CG
NarI 480 GGCGCC GG^CGCC
NdeII 315 GATC ^GATC
NheI 102 GCTAGC G^CTAGC
PflMI 747 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 581 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 751 CAGCTG CAG^CTG
RsaI 207 GTAC GT^AC
Sau3AI 315 GATC ^GATC
SfoI 480 GGCGCC GGC^GCC
SspBI 206 TGTACA T^GTACA
Tsp509I 435 AATT ^AATT
Van91I 747 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 170 CCACTTGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 420 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 582 GGACC G^G[AT]CC
687 GGACC
BanI 410 GGTGCC G^G[CT][GA]CC
480 GGCGCC
BanII 43 GGGCCC G[GA]GC[CT]^C
513 GGGCTC
BpmI 544 CTGGAG CTGGAG(16/14)
721 CTGGAG
BsaWI 443 ACCGGA [AT]^CCGG[AT]
689 ACCGGA
BseRI 94 GAGGAG GAGGAG(10/8)
133 GAGGAG
BsgI 268 CTGCAC GTGCAG(16/14)
532 CTGCAC
BssSI 577 CACGAG CACGAG(-5/-1)
16 CTCGTG
DraII 581 AGGACCT [GA]G^G[ACGT]CC[CT]
716 GGGGCCT
EcoO109I 581 AGGACCT [GA]G^G[ACGT]CC[CT]
716 GGGGCCT
HaeII 480 GGCGCC [GA]GCGC^[CT]
540 GGCGCT
MaeIII 362 GTGAC ^GT[ACGT]AC
644 GTCAC
NlaIII 300 CATG CATG^
400 CATG
PstI 337 CTGCAG CTGCA^G
415 CTGCAG
SmaI 46 CCCGGG CCC^GGG
485 CCCGGG
Tsp45I 362 GTGAC ^GT[GC]AC
644 GTCAC
XmaCI 46 CCCGGG C^CCGGG
485 CCCGGG
XmaI 46 CCCGGG C^CCGGG
485 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MnlI
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I