(Circular) (Six-base) MAPSORT of: pFN21AB5337.seq Check: 5807 from: 1 to: 5479 pFN21AB5337 5479 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2403 2403 Size: 5479 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 1937 Size: 885 4594 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 3606 3979 1563 Size: 2043 373 3063 Fragments arranged by size: 3063 2043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2371 3542 4926 5194 1632 Size: 739 1171 1384 268 1917 Fragments arranged by size: 1917 1384 1171 739 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 4378 5355 1203 Size: 3175 977 1327 Fragments arranged by size: 3175 1327 977 AgeI A'CCGG_T Cuts at: 4310 4310 Size: 5479 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 1184 Size: 1251 4228 AlwNI CAG_nnn'CTG Cuts at: 2394 3222 4794 2394 Size: 828 1572 3079 Fragments arranged by size: 3079 1572 828 ApaI G_GGCC'C Cuts at: 2052 2052 Size: 5479 ApaLI G'TGCA_C Cuts at: 3536 4692 3536 Size: 1156 4323 ApoI r'AATT_y Cuts at: 1493 2439 2788 3014 3166 5275 1493 Size: 946 349 226 152 2109 1697 Fragments arranged by size: 2109 1697 946 349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2230 2253 2490 2804 1870 Size: 73 108 179 23 237 314 4545 Fragments arranged by size: 4545 314 237 179 108 73 23 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2415 2485 2800 4255 618 Size: 325 217 627 628 70 315 1455 1842 Fragments arranged by size: 1842 1455 628 627 325 315 217 70 BanII G_rGCy'C Cuts at: 1391 2052 2522 2798 1391 Size: 661 470 276 4072 Fragments arranged by size: 4072 661 470 276 BbeI G_GCGC'C Cuts at: 2489 2489 Size: 5479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2705 928 Size: 971 806 3702 Fragments arranged by size: 3702 971 806 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3670 3704 1903 Size: 34 50 34 1649 34 3678 Fragments arranged by size: 3678 1649 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4090 136 Size: 107 122 71 631 605 2418 1525 Fragments arranged by size: 2418 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5474 5474 Size: 5479 BlpI GC'TnA_GC Cuts at: 2896 2896 Size: 5479 Bme1580I G_kGCm'C Cuts at: 1790 2052 2404 2514 3540 4696 1790 Size: 262 352 110 1026 1156 2573 Fragments arranged by size: 2573 1156 1026 352 262 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3210 4168 442 Size: 416 53 83 399 24 1793 958 1753 Fragments arranged by size: 1793 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2111 1056 Size: 1055 4424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2747 4139 1239 Size: 1331 177 1392 2579 Fragments arranged by size: 2579 1392 1331 177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3604 4469 4767 3604 Size: 865 298 4316 Fragments arranged by size: 4316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 4142 882 Size: 617 940 1703 2219 Fragments arranged by size: 2219 1703 940 617 BsaAI yAC'GTr Cuts at: 493 1204 3270 493 Size: 711 2066 2702 Fragments arranged by size: 2702 2066 711 BsaBI GATnn'nnATC Cuts at: 5473 5473 Size: 5479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2486 3286 3668 4303 Size: 53 83 186 936 953 800 382 635 Cuts at: 4303 275 Size: 1451 Fragments arranged by size: 1451 953 936 800 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2448 2694 3912 4310 4584 4731 5142 2448 Size: 246 1218 398 274 147 411 2785 Fragments arranged by size: 2785 1218 411 398 274 246 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2443 2473 1178 Size: 30 165 30 1040 30 4184 Fragments arranged by size: 4184 1040 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2153 1186 Size: 928 39 4512 Fragments arranged by size: 4512 928 39 BseYI C'CCAG_C Cuts at: 2653 4682 5178 2653 Size: 2029 496 2954 Fragments arranged by size: 2954 2029 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 2522 1163 Size: 573 522 264 4120 Fragments arranged by size: 4120 573 522 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2620 2929 3690 4718 1540 Size: 275 186 619 309 761 1028 2301 Fragments arranged by size: 2301 1028 761 619 309 275 186 BsiHKAI G_wGCw'C Cuts at: 2456 3540 3625 4696 2456 Size: 1084 85 1071 3239 Fragments arranged by size: 3239 1084 1071 85 BsmI GAATG_Cn' Cuts at: 2990 3083 2990 Size: 93 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2404 2456 2514 2522 2798 3540 Size: 399 262 352 52 58 8 276 742 Cuts at: 3540 3625 4696 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 276 262 85 58 52 8 BspEI T'CCGG_A Cuts at: 5142 5142 Size: 5479 BspHI T'CATG_A Cuts at: 3368 5098 3368 Size: 1730 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 2834 844 Size: 1559 431 3489 Fragments arranged by size: 3489 1559 431 BsrBI CCG'CTC Cuts at: 1394 1823 2554 3366 1394 Size: 429 731 812 3507 Fragments arranged by size: 3507 812 731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3974 4148 65 Size: 1183 2726 174 1396 Fragments arranged by size: 2726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4123 4310 1700 Size: 110 2313 187 2869 Fragments arranged by size: 2869 2313 187 110 BsrGI T'GTAC_A Cuts at: 96 2211 96 Size: 2115 3364 BssHII G'CGCG_C Cuts at: 1925 2111 1925 Size: 186 5293 BssSI C'ACGA_G Cuts at: 2021 2582 3539 4551 2021 Size: 561 957 1012 2949 Fragments arranged by size: 2949 1012 957 561 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5479 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 2366 1184 Size: 1182 4297 BstYI r'GATC_y Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474 Size: 837 367 385 17 1452 86 12 346 Cuts at: 5474 1972 Size: 1977 Fragments arranged by size: 1977 1452 837 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2127 513 Size: 552 1062 3865 Fragments arranged by size: 3865 1062 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4740 Fragments arranged by size: 4740 683 56 BtsI GCAGTG_nn' Cuts at: 2080 2651 2995 3789 3809 2080 Size: 571 344 794 20 3750 Fragments arranged by size: 3750 794 571 344 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5444 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4486 809 Size: 507 3170 1802 Fragments arranged by size: 3170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2040 2559 Size: 54 1425 50 275 18 195 15 519 Cuts at: 2559 2926 3815 5444 8 Size: 367 889 1629 43 Fragments arranged by size: 1629 1425 889 519 367 275 195 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2926 1537 Size: 275 1114 4090 Fragments arranged by size: 4090 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3409 1353 Size: 2056 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 4066 4440 4586 387 Size: 1064 138 741 1736 374 146 1280 Fragments arranged by size: 1736 1280 1064 741 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2371 2570 2747 3542 4139 4926 5194 Size: 393 739 199 177 795 597 787 268 Cuts at: 5194 1239 Size: 1524 Fragments arranged by size: 1524 795 787 739 597 393 268 199 177 EcoO109I rG'GnC_Cy Cuts at: 2587 2722 2587 Size: 135 5344 EcoRI G'AATT_C Cuts at: 2788 3166 2788 Size: 378 5101 FspI TGC'GCA Cuts at: 3985 3985 Size: 5479 HaeII r_GCGC'y Cuts at: 1678 2489 2549 4626 1678 Size: 811 60 2077 2531 Fragments arranged by size: 2531 2077 811 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4120 4152 4194 4226 1373 Size: 32 135 32 2548 32 42 32 2626 Fragments arranged by size: 2626 2548 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 3069 669 Size: 988 281 885 246 3079 Fragments arranged by size: 3079 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 2711 748 Size: 1963 3516 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KasI G'GCGC_C Cuts at: 2485 2485 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 5355 5355 Size: 5479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4593 4777 1305 Size: 3288 184 2007 Fragments arranged by size: 3288 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3657 Fragments arranged by size: 3657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 3437 3796 3955 518 Size: 648 1116 1155 359 159 2042 Fragments arranged by size: 2042 1155 1116 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2758 2895 3572 4720 4965 1606 Size: 1152 137 677 1148 245 2120 Fragments arranged by size: 2120 1152 1148 677 245 137 NarI GG'CG_CC Cuts at: 2486 2486 Size: 5479 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4927 NdeI CA'TA_TG Cuts at: 387 387 Size: 5479 NheI G'CTAG_C Cuts at: 1052 2107 1052 Size: 1055 4424 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2837 4382 1132 Size: 97 1608 1545 2229 Fragments arranged by size: 2229 1608 1545 97 PasI CC'CwG_GG Cuts at: 1348 2185 2515 1348 Size: 837 330 4312 Fragments arranged by size: 4312 837 330 PciI A'CATG_T Cuts at: 4378 4378 Size: 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 2758 1108 Size: 1650 3829 PfoI T'CCnGG_A Cuts at: 2331 2331 Size: 5479 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3580 5061 5093 3548 Size: 32 1481 32 3934 Fragments arranged by size: 3934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 2587 Size: 5479 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PspOMI G'GGCC_C Cuts at: 2048 2048 Size: 5479 PstI C_TGCA'G Cuts at: 830 2346 2424 2831 830 Size: 1516 78 407 3478 Fragments arranged by size: 3478 1516 407 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 PvuII CAG'CTG Cuts at: 2758 2758 Size: 5479 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 3727 5439 3727 Size: 1712 3767 SfcI C'TryA_G Cuts at: 826 1045 2342 2395 2420 2827 3962 4643 4834 Size: 219 1297 53 25 407 1135 681 191 Cuts at: 4834 826 Size: 1471 Fragments arranged by size: 1471 1297 1135 681 407 219 191 53 25 SfoI GGC'GCC Cuts at: 2487 2487 Size: 5479 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5479 SmaI CCC'GGG Cuts at: 1872 2053 2492 2806 1872 Size: 181 439 314 4545 Fragments arranged by size: 4545 439 314 181 SmlI C'TyrA_G Cuts at: 820 1017 1943 3583 4484 4746 820 Size: 197 926 1640 901 262 1553 Fragments arranged by size: 1640 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SrfI GCCC'GGGC Cuts at: 2053 2053 Size: 5479 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5479 StyI C'CwwG_G Cuts at: 513 1065 2918 513 Size: 552 1853 3074 Fragments arranged by size: 3074 1853 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 2667 3506 3523 3676 3861 4306 Size: 1255 1014 73 839 17 153 185 445 Cuts at: 4306 325 Size: 1498 Fragments arranged by size: 1498 1255 1014 839 445 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3725 5437 Size: 274 80 33 51 1443 234 1514 1712 Cuts at: 5437 96 Size: 138 Fragments arranged by size: 1712 1514 1443 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2443 2475 5439 5471 2443 Size: 32 2964 32 2451 Fragments arranged by size: 2964 2451 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5479 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 2182 Size: 5479 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2051 2490 2804 1870 Size: 181 439 314 4545 Fragments arranged by size: 4545 439 314 181 XmnI GAAnn'nnTTC Cuts at: 2429 3608 2429 Size: 1179 4300 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI