Restriction map for
pFN21AB7918
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I RsrII SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AhdI 252 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 404 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 939 CCTGAGG CC^T[ACGT]AGG
ApaI 378 GGGCCC GGGCC^C
AspEI 252 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 934 GAGCTC G[AT]GC[AT]^C
BalI 146 TGGCCA TGG^CCA
BbeI 289 GGCGCC GGCGC^C
BsaI 895 GAGACC GGTCTC(1/5)
BsiHKAI 934 GAGCTC G[AT]GC[AT]^C
BspHI 593 TCATGA T^CATGA
BsrDI 452 GCAATG GCAATG(2/0)
BstAPI 493 GCACGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 774 TTCGAA TT^CGAA
Bsu36I 939 CCTGAGG CC^T[ACGT]AGG
DdeI 940 CTGAG C^T[ACGT]AG
EagI 470 CGGCCG C^GGCCG
Eam1105I 252 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 707 GAAGAG CTCTTC(1/4)
EclXI 470 CGGCCG C^GGCCG
Eco52I 470 CGGCCG C^GGCCG
Eco81I 939 CCTGAGG CC^T[ACGT]AGG
EcoNI 837 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 842 CCAAGG C^C[AT][AT]GG
KasI 289 GGCGCC G^GCGCC
Ksp632I 707 GAAGAG CTCTTC(1/4)
MaeII 590 ACGT A^CGT
MluNI 146 TGGCCA TGG^CCA
MscI 146 TGGCCA TGG^CCA
NarI 289 GGCGCC GG^CGCC
NspV 774 TTCGAA TT^CGAA
PshAI 13 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 638 CGATCG CGAT^CG
RcaI 593 TCATGA T^CATGA
SacI 934 GAGCTC GAGCT^C
SapI 707 GAAGAGC GCTCTTC(1/4)
SfoI 289 GGCGCC GGC^GCC
SfuI 774 TTCGAA TT^CGAA
SphI 887 GCATGC GCATG^C
StuI 845 AGGCCT AGG^CCT
StyI 842 CCAAGG C^C[AT][AT]GG
Tsp509I 672 AATT ^AATT
TspRI 519 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 246 CCAGGTGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 238 CGCG CG^CG
321 CGCG
AfaI 195 GTAC GT^AC
547 GTAC
BamHI 24 GGATCC G^GATCC
833 GGATCC
BanI 289 GGCGCC G^G[CT][GA]CC
1027 GGCACC
BpmI 575 CTGGAG CTGGAG(16/14)
1009 CTGGAG
BsiEI 470 CGGCCG CG[GA][CT]^CG
638 CGATCG
BsmAI 211 GAGAC GTCTC(1/5)
895 GAGAC
BstUI 238 CGCG CG^CG
321 CGCG
BstYI 24 GGATCC [GA]^GATC[CT]
833 GGATCC
MboII 707 GAAGA GAAGA(8/7)
963 GAAGA
MflI 24 GGATCC [GA]^GATC[CT]
833 GGATCC
MvnI 238 CGCG CG^CG
321 CGCG
NspI 5 ACATGC [GA]CATG^[CT]
887 GCATGC
PpuMI 72 GGGACCC [GA]G^G[AT]CC[CT]
405 AGGTCCT
RsaI 195 GTAC GT^AC
547 GTAC
SmaI 15 CCCGGG CCC^GGG
375 CCCGGG
XhoII 24 GGATCC [GA]^GATC[CT]
833 GGATCC
XmaCI 15 CCCGGG C^CCGGG
375 CCCGGG
XmaI 15 CCCGGG C^CCGGG
375 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaI
AvaII BanII BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfcI TaqI TseI Tsp45I TthHB8I