(Circular) (Six-base) MAPSORT of: pFN21AE1055.seq Check: 5346 from: 1 to: 9445 pFN21AE1055 9445 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3540 4463 4986 3540 Size: 923 523 7999 Fragments arranged by size: 7999 923 523 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2942 4518 7255 278 Size: 53 83 186 936 1406 1576 2737 2468 Fragments arranged by size: 2737 2468 1576 1406 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6788 1937 Size: 4851 4594 Acc65I G'GTAC_C Cuts at: 5967 6766 5967 Size: 799 8646 AclI AA'CG_TT Cuts at: 1563 7572 7945 1563 Size: 6009 373 3063 Fragments arranged by size: 6009 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2557 2906 2973 3447 4883 5059 5126 5867 Size: 925 349 67 474 1436 176 67 741 Cuts at: 5867 6337 7508 8892 9160 1632 Size: 470 1171 1384 268 1917 Fragments arranged by size: 1917 1436 1384 1171 925 741 474 470 349 268 176 67 67 AfeI AGC'GCT Cuts at: 6651 6651 Size: 9445 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9248 AflIII A'CryG_T Cuts at: 1203 2290 3883 8344 9321 1203 Size: 1087 1593 4461 977 1327 Fragments arranged by size: 4461 1593 1327 1087 977 AgeI A'CCGG_T Cuts at: 8276 8276 Size: 9445 AhdI GACnn_n'nnGTC Cuts at: 8174 8174 Size: 9445 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5153 5451 5876 6066 1184 Size: 3969 298 425 190 4563 Fragments arranged by size: 4563 3969 425 298 190 AlwNI CAG_nnn'CTG Cuts at: 5091 7188 8760 5091 Size: 2097 1572 5776 Fragments arranged by size: 5776 2097 1572 ApaI G_GGCC'C Cuts at: 4473 5382 6571 4473 Size: 909 1189 7347 Fragments arranged by size: 7347 1189 909 ApaLI G'TGCA_C Cuts at: 3497 5802 7502 8658 3497 Size: 2305 1700 1156 4284 Fragments arranged by size: 4284 2305 1700 1156 ApoI r'AATT_y Cuts at: 1493 2056 5305 5884 6725 6754 6980 7132 9241 Size: 563 3249 579 841 29 226 152 2109 Cuts at: 9241 1493 Size: 1697 Fragments arranged by size: 3249 2109 1697 841 579 563 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 2595 7999 160 Size: 872 1563 5404 1606 Fragments arranged by size: 5404 1606 1563 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9445 AvaI C'yCGr_G Cuts at: 1870 1943 3348 3676 3961 4187 4486 4883 4895 Size: 73 1405 328 285 226 299 397 12 Cuts at: 4895 4930 5159 5399 5441 5456 5651 6007 6627 Size: 35 229 240 42 15 195 356 620 Cuts at: 6627 6770 1870 Size: 143 4545 Fragments arranged by size: 4545 1405 620 397 356 328 299 285 240 229 226 195 143 73 42 35 15 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5951 5984 5951 Size: 33 9412 BamHI G'GATC_C Cuts at: 3189 6775 7142 3189 Size: 3586 367 5492 Fragments arranged by size: 5492 3586 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4971 5717 5813 5822 5967 Size: 325 217 627 3184 746 96 9 145 Cuts at: 5967 6183 6766 8221 618 Size: 216 583 1455 1842 Fragments arranged by size: 3184 1842 1455 746 627 583 325 217 216 145 96 9 BanII G_rGCy'C Cuts at: 1391 2208 2304 2364 2675 3349 4021 4188 4473 Size: 817 96 60 311 674 672 167 285 Cuts at: 4473 4957 5382 6106 6571 6578 6764 1391 Size: 484 425 724 465 7 186 4072 Fragments arranged by size: 4072 817 724 674 672 484 465 425 311 285 186 167 96 60 7 BbeI G_GCGC'C Cuts at: 4975 6187 4975 Size: 1212 8233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3333 3625 3896 4128 4871 928 Size: 971 1434 292 271 232 743 5502 Fragments arranged by size: 5502 1434 971 743 292 271 232 BbvCI CC'TCA_GC Cuts at: 2773 4040 5084 2773 Size: 1267 1044 7134 Fragments arranged by size: 7134 1267 1044 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 7636 7670 1903 Size: 34 2882 34 2783 34 3678 Fragments arranged by size: 3678 2882 2783 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2517 7337 8547 2517 Size: 4820 1210 3415 Fragments arranged by size: 4820 3415 1210 BclI T'GATC_A Cuts at: 1548 2259 1548 Size: 711 8734 BfrBI ATG'CAT Cuts at: 3082 3082 Size: 9445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5377 5421 Size: 107 122 71 631 605 337 3368 44 Cuts at: 5421 5460 8056 136 Size: 39 2596 1525 Fragments arranged by size: 3368 2596 1525 631 605 337 122 107 71 44 39 BglII A'GATC_T Cuts at: 3707 9440 3707 Size: 5733 3712 BlpI GC'TnA_GC Cuts at: 2207 2669 4760 6862 2207 Size: 462 2091 2102 4790 Fragments arranged by size: 4790 2102 2091 462 Bme1580I G_kGCm'C Cuts at: 1790 3501 4473 5373 5382 5806 6571 7506 8662 Size: 1711 972 900 9 424 765 935 1156 Cuts at: 8662 1790 Size: 2573 Fragments arranged by size: 2573 1711 1156 972 935 900 765 424 9 BmgBI CAC'GTC Cuts at: 1318 5115 5290 1318 Size: 3797 175 5473 Fragments arranged by size: 5473 3797 175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2008 3463 3586 Size: 416 53 83 399 24 591 1455 123 Cuts at: 3586 5948 7176 8134 442 Size: 2362 1228 958 1753 Fragments arranged by size: 2362 1753 1455 1228 958 591 416 399 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9445 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 9445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2827 3738 4058 4603 4615 4705 4902 Size: 856 732 911 320 545 12 90 197 Cuts at: 4902 5249 6535 8105 1239 Size: 347 1286 1570 2579 Fragments arranged by size: 2579 1570 1286 911 856 732 545 347 320 197 90 12 Bpu10I CC'TnA_GC Cuts at: 2773 3095 3112 4040 4580 5084 6572 2773 Size: 322 17 928 540 504 1488 5646 Fragments arranged by size: 5646 1488 928 540 504 322 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3061 3550 3724 4775 5519 5638 7570 8435 8733 Size: 489 174 1051 744 119 1932 865 298 Cuts at: 8733 3061 Size: 3773 Fragments arranged by size: 3773 1932 1051 865 744 489 298 174 119 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4215 4420 8108 882 Size: 617 2716 205 3688 2219 Fragments arranged by size: 3688 2716 2219 617 205 BsaAI yAC'GTr Cuts at: 493 1204 2293 2505 4336 7236 493 Size: 711 1089 212 1831 2900 2702 Fragments arranged by size: 2900 2702 1831 1089 711 212 BsaBI GATnn'nnATC Cuts at: 9439 9439 Size: 9445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2939 4515 4640 4707 Size: 53 83 186 936 1406 1576 125 67 Cuts at: 4707 4832 4972 6184 6396 6632 7252 7634 8269 Size: 125 140 1212 212 236 620 382 635 Cuts at: 8269 275 Size: 1451 Fragments arranged by size: 1576 1451 1406 1212 936 635 620 382 236 212 186 140 125 125 83 67 53 BsaWI w'CCGG_w Cuts at: 2509 2541 3876 5504 7878 8276 8550 8697 9108 Size: 32 1335 1628 2374 398 274 147 411 Cuts at: 9108 2509 Size: 2846 Fragments arranged by size: 2846 2374 1628 1335 411 398 274 147 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 3530 3560 3854 Size: 30 165 30 597 30 1500 30 294 Cuts at: 3854 3884 4682 4712 5507 5537 1178 Size: 30 798 30 795 30 5086 Fragments arranged by size: 5086 1500 798 795 597 294 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2367 4410 4585 4948 5026 5515 5635 5975 Size: 1181 2043 175 363 78 489 120 340 Cuts at: 5975 6598 1186 Size: 623 4033 Fragments arranged by size: 4033 2043 1181 623 489 363 340 175 120 78 BseYI C'CCAG_C Cuts at: 2704 3335 4751 4776 5204 5342 5633 6646 8648 Size: 631 1416 25 428 138 291 1013 2002 Cuts at: 8648 9144 2704 Size: 496 3005 Fragments arranged by size: 3005 2002 1416 1013 631 496 428 291 138 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3568 3829 4960 5315 1163 Size: 573 1832 261 1131 355 5293 Fragments arranged by size: 5293 1832 1131 573 355 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3953 4007 4661 6895 7656 8684 Size: 275 186 1952 54 654 2234 761 1028 Cuts at: 8684 1540 Size: 2301 Fragments arranged by size: 2301 2234 1952 1028 761 654 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2075 3501 3744 4335 5688 5806 5855 6305 6665 Size: 1426 243 591 1353 118 49 450 360 Cuts at: 6665 7506 7591 8662 2075 Size: 841 85 1071 2858 Fragments arranged by size: 2858 1426 1353 1071 841 591 450 360 243 118 85 49 BsmI GAATG_Cn' Cuts at: 3805 4073 6956 7049 3805 Size: 268 2883 93 6201 Fragments arranged by size: 6201 2883 268 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2946 3073 2946 Size: 127 9318 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2208 2304 2364 2675 3349 3501 Size: 399 285 133 96 60 311 674 152 Cuts at: 3501 3744 4021 4188 4335 4473 4957 5373 5382 Size: 243 277 167 147 138 484 416 9 Cuts at: 5382 5688 5806 5855 6106 6305 6571 6578 6665 Size: 306 118 49 251 199 266 7 87 Cuts at: 6665 6764 7506 7591 8662 1391 Size: 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 484 416 399 311 306 285 277 266 251 243 199 167 152 147 138 133 118 99 96 87 85 60 49 9 7 BspEI T'CCGG_A Cuts at: 2509 3876 5504 9108 2509 Size: 1367 1628 3604 2846 Fragments arranged by size: 3604 2846 1628 1367 BspHI T'CATG_A Cuts at: 2428 7334 9064 2428 Size: 4906 1730 2809 Fragments arranged by size: 4906 2809 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2523 3540 4463 4986 5484 6257 6800 844 Size: 1679 1017 923 523 498 773 543 3489 Fragments arranged by size: 3489 1679 1017 923 773 543 523 498 BsrBI CCG'CTC Cuts at: 1394 1823 2619 4593 4629 4719 5625 5871 7332 Size: 429 796 1974 36 90 906 246 1461 Cuts at: 7332 1394 Size: 3507 Fragments arranged by size: 3507 1974 1461 906 796 429 246 90 36 BsrDI GCAATG_nn' Cuts at: 65 1248 2468 7940 8114 65 Size: 1183 1220 5472 174 1396 Fragments arranged by size: 5472 1396 1220 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2786 4656 5896 8089 8276 1700 Size: 110 976 1870 1240 2193 187 2869 Fragments arranged by size: 2869 2193 1870 1240 976 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9445 BssSI C'ACGA_G Cuts at: 3256 5061 5135 7505 8517 3256 Size: 1805 74 2370 1012 4184 Fragments arranged by size: 4184 2370 1805 1012 74 BstAPI GCAn_nnn'nTGC Cuts at: 794 3496 5692 794 Size: 2702 2196 4547 Fragments arranged by size: 4547 2702 2196 BstBI TT'CG_AA Cuts at: 8249 8249 Size: 9445 BstEII G'GTnAC_C Cuts at: 1184 3284 1184 Size: 2100 7345 BstXI CCAn_nnnn'nTGG Cuts at: 2806 2953 3524 4327 6673 2806 Size: 147 571 803 2346 5578 Fragments arranged by size: 5578 2346 803 571 147 BstYI r'GATC_y Cuts at: 1972 2271 2529 3090 3189 3707 4602 6775 7142 Size: 299 258 561 99 518 895 2173 367 Cuts at: 7142 7527 7544 8996 9082 9094 9440 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2173 1977 1452 895 561 518 385 367 346 299 258 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4597 4597 Size: 9445 BtgI C'CryG_G Cuts at: 513 1065 2004 2275 2317 2334 2662 4957 5002 Size: 552 939 271 42 17 328 2295 45 Cuts at: 5002 5118 5241 5374 5546 6345 513 Size: 116 123 133 172 799 3613 Fragments arranged by size: 3613 2295 939 799 552 328 271 172 133 123 116 45 42 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2622 5120 487 Size: 683 56 1396 2498 4812 Fragments arranged by size: 4812 2498 1396 683 56 BtsI GCAGTG_nn' Cuts at: 3276 4269 6961 7755 7775 3276 Size: 993 2692 794 20 4946 Fragments arranged by size: 4946 2692 993 794 20 ClaI AT'CG_AT Cuts at: 1554 4236 1554 Size: 2682 6763 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4267 4302 6456 6491 529 Size: 35 3703 35 2154 35 3483 Fragments arranged by size: 3703 3483 2154 35 35 35 DraI TTT'AAA Cuts at: 5304 6749 5304 Size: 1445 8000 DraIII CAC_nnn'GTG Cuts at: 5008 5008 Size: 9445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8452 809 Size: 507 7136 1802 Fragments arranged by size: 7136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 3950 4000 Size: 54 1425 50 275 18 910 1210 50 Cuts at: 4000 4658 4677 5109 5167 5422 6892 7781 9410 Size: 658 19 432 58 255 1470 889 1629 Cuts at: 9410 8 Size: 43 Fragments arranged by size: 1629 1470 1425 1210 910 889 658 432 275 255 58 54 50 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 3950 4658 6892 1537 Size: 275 2138 708 2234 4090 Fragments arranged by size: 4090 2234 2138 708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2992 3061 5843 7375 1353 Size: 1639 69 2782 1532 3423 Fragments arranged by size: 3423 2782 1639 1532 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2312 2473 8032 8406 8552 387 Size: 1064 138 723 161 5559 374 146 1280 Fragments arranged by size: 5559 1280 1064 723 374 161 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2557 2827 2906 2973 3447 3738 Size: 393 463 462 270 79 67 474 291 Cuts at: 3738 4058 4603 4615 4705 4883 4902 5059 5126 Size: 320 545 12 90 178 19 157 67 Cuts at: 5126 5249 5867 6337 6535 7508 8105 8892 9160 Size: 123 618 470 198 973 597 787 268 Cuts at: 9160 1239 Size: 1524 Fragments arranged by size: 1524 973 787 618 597 545 474 470 463 462 393 320 291 270 268 198 178 157 123 90 79 67 67 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 2524 3183 3386 4425 6410 2524 Size: 659 203 1039 1985 5559 Fragments arranged by size: 5559 1985 1039 659 203 EcoO109I rG'GnC_Cy Cuts at: 2250 2280 4036 5047 5755 5950 6567 6568 2250 Size: 30 1756 1011 708 195 617 1 5127 Fragments arranged by size: 5127 1756 1011 708 617 195 30 1 EcoRI G'AATT_C Cuts at: 2056 6754 7132 2056 Size: 4698 378 4369 Fragments arranged by size: 4698 4369 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3509 3509 Size: 9445 FseI GG_CCGG'CC Cuts at: 4660 4660 Size: 9445 FspI TGC'GCA Cuts at: 7951 7951 Size: 9445 HaeII r_GCGC'y Cuts at: 1678 4557 4880 4975 5349 5514 5906 6187 6426 Size: 2879 323 95 374 165 392 281 239 Cuts at: 6426 6653 8592 1678 Size: 227 1939 2531 Fragments arranged by size: 2879 2531 1939 392 374 323 281 239 227 165 95 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 3854 3886 3924 Size: 32 135 32 428 32 1822 32 38 Cuts at: 3924 3956 4682 4714 5507 5539 8086 8118 8160 Size: 32 726 32 793 32 2547 32 42 Cuts at: 8160 8192 1373 Size: 32 2626 Fragments arranged by size: 2626 2547 1822 793 726 428 135 42 38 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6789 7035 669 Size: 988 281 4851 246 3079 Fragments arranged by size: 4851 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 6130 748 Size: 5382 4063 HpaI GTT'AAC Cuts at: 7035 7035 Size: 9445 KasI G'GCGC_C Cuts at: 4971 6183 4971 Size: 1212 8233 KpnI G_GTAC'C Cuts at: 5971 6770 5971 Size: 799 8646 MfeI C'AATT_G Cuts at: 2412 7044 2412 Size: 4632 4813 MluI A'CGCG_T Cuts at: 9321 9321 Size: 9445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3191 4200 4470 8559 8743 1305 Size: 1886 1009 270 4089 184 2007 Fragments arranged by size: 4089 2007 1886 1009 270 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2742 5111 5424 10 Size: 54 1425 343 910 2369 313 4031 Fragments arranged by size: 4031 2369 1425 910 343 313 54 MslI CAynn'nnrTG Cuts at: 518 1166 2804 3522 3810 3826 5858 6671 7403 Size: 648 1638 718 288 16 2032 813 732 Cuts at: 7403 7762 7921 518 Size: 359 159 2042 Fragments arranged by size: 2042 2032 1638 813 732 718 648 359 288 159 16 MspA1I CmG'CkG Cuts at: 1606 2319 2552 2777 5091 5120 5548 5555 5881 Size: 713 233 225 2314 29 428 7 326 Cuts at: 5881 5918 6298 6402 6861 7538 8686 8931 1606 Size: 37 380 104 459 677 1148 245 2120 Fragments arranged by size: 2314 2120 1148 713 677 459 428 380 326 245 233 225 104 37 29 7 NaeI GCC'GGC Cuts at: 2788 4658 2788 Size: 1870 7575 NarI GG'CG_CC Cuts at: 4972 6184 4972 Size: 1212 8233 NcoI C'CATG_G Cuts at: 513 1065 2004 2275 2334 5241 513 Size: 552 939 271 59 2907 4717 Fragments arranged by size: 4717 2907 939 552 271 59 NdeI CA'TA_TG Cuts at: 387 2475 3612 3823 387 Size: 2088 1137 211 6009 Fragments arranged by size: 6009 2088 1137 211 NgoMIV G'CCGG_C Cuts at: 2786 4656 2786 Size: 1870 7575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9445 NotI GC'GGCC_GC Cuts at: 6892 6892 Size: 9445 NsiI A_TGCA'T Cuts at: 3084 3084 Size: 9445 NspI r_CATG'y Cuts at: 1132 1229 3082 3887 4534 5941 6001 6803 8348 Size: 97 1853 805 647 1407 60 802 1545 Cuts at: 8348 1132 Size: 2229 Fragments arranged by size: 2229 1853 1545 1407 805 802 647 97 60 PasI CC'CwG_GG Cuts at: 1348 3341 1348 Size: 1993 7452 PciI A'CATG_T Cuts at: 3883 8344 3883 Size: 4461 4984 PflMI CCAn_nnn'nTGG Cuts at: 1108 4124 5487 6346 6499 1108 Size: 3016 1363 859 153 4054 Fragments arranged by size: 4054 3016 1363 859 153 PfoI T'CCnGG_A Cuts at: 2140 5352 2140 Size: 3212 6233 PmeI GTTT'AAAC Cuts at: 6749 6749 Size: 9445 PmlI CAC'GTG Cuts at: 2293 2293 Size: 9445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3100 3132 7514 7546 9027 9059 3100 Size: 32 4382 32 1481 32 3486 Fragments arranged by size: 4382 3486 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2280 4036 5950 2280 Size: 1756 1914 5775 Fragments arranged by size: 5775 1914 1756 PshAI GACnn'nnGTC Cuts at: 1613 6240 1613 Size: 4627 4818 PsiI TTA'TAA Cuts at: 7015 7015 Size: 9445 PspOMI G'GGCC_C Cuts at: 4469 5378 6567 4469 Size: 909 1189 7347 Fragments arranged by size: 7347 1189 909 PspXI vC'TCGA_Gb Cuts at: 4187 4895 5441 4187 Size: 708 546 8191 Fragments arranged by size: 8191 708 546 PstI C_TGCA'G Cuts at: 830 3271 4460 4983 6219 6254 6315 6797 830 Size: 2441 1189 523 1236 35 61 482 3478 Fragments arranged by size: 3478 2441 1236 1189 523 482 61 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9445 PvuII CAG'CTG Cuts at: 2777 5091 5881 5918 6402 2777 Size: 2314 790 37 484 5820 Fragments arranged by size: 5820 2314 790 484 37 SacII CC_GC'GG Cuts at: 2320 5121 5549 2320 Size: 2801 428 6216 Fragments arranged by size: 6216 2801 428 SalI G'TCGA_C Cuts at: 1936 6787 1936 Size: 4851 4594 SanDI GG'GwC_CC Cuts at: 2280 2280 Size: 9445 SapI GCTCTTCn'nnn_ Cuts at: 2992 5843 2992 Size: 2851 6594 SbfI CC_TGCA'GG Cuts at: 6254 6797 6254 Size: 543 8902 ScaI AGT'ACT Cuts at: 7693 9405 7693 Size: 1712 7733 SfcI C'TryA_G Cuts at: 826 1045 2676 3267 4456 4979 6215 6250 6311 Size: 219 1631 591 1189 523 1236 35 61 Cuts at: 6311 6410 6793 7928 8609 8800 826 Size: 99 383 1135 681 191 1471 Fragments arranged by size: 1631 1471 1236 1189 1135 681 591 523 383 219 191 99 61 35 SfoI GGC'GCC Cuts at: 4973 6185 4973 Size: 1212 8233 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9445 SmaI CCC'GGG Cuts at: 1872 4488 5401 6009 6772 1872 Size: 2616 913 608 763 4545 Fragments arranged by size: 4545 2616 913 763 608 SmlI C'TyrA_G Cuts at: 820 1017 1943 3040 3565 3676 3703 4187 4790 Size: 197 926 1097 525 111 27 484 603 Cuts at: 4790 4895 5441 5534 5617 7549 8450 8712 820 Size: 105 546 93 83 1932 901 262 1553 Fragments arranged by size: 1932 1553 1097 926 901 603 546 525 484 262 197 111 105 93 83 27 SnaBI TAC'GTA Cuts at: 493 2505 7236 493 Size: 2012 4731 2702 Fragments arranged by size: 4731 2702 2012 SpeI A'CTAG_T Cuts at: 152 152 Size: 9445 SphI G_CATG'C Cuts at: 1132 5941 6001 6803 1132 Size: 4809 60 802 3774 Fragments arranged by size: 4809 3774 802 60 SrfI GCCC'GGGC Cuts at: 5401 5401 Size: 9445 SspI AAT'ATT Cuts at: 5 52 7369 5 Size: 47 7317 2081 Fragments arranged by size: 7317 2081 47 StuI AGG'CCT Cuts at: 1517 3178 3671 1517 Size: 1661 493 7291 Fragments arranged by size: 7291 1661 493 StyI C'CwwG_G Cuts at: 513 1065 2004 2275 2334 2952 4021 5241 6076 Size: 552 939 271 59 618 1069 1220 835 Cuts at: 6076 6884 513 Size: 808 3074 Fragments arranged by size: 3074 1220 1069 939 835 808 618 552 271 59 SwaI ATTT'AAAT Cuts at: 5304 5304 Size: 9445 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3497 4021 4217 4454 7472 7489 7642 Size: 1255 1917 524 196 237 3018 17 153 Cuts at: 7642 7827 8272 325 Size: 185 445 1498 Fragments arranged by size: 3018 1917 1498 1255 524 445 237 196 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7691 9403 96 Size: 274 80 33 51 1443 5714 1712 138 Fragments arranged by size: 5714 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2181 2213 3530 3562 9405 9437 2181 Size: 32 1317 32 5843 32 2189 Fragments arranged by size: 5843 2189 1317 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9445 XbaI T'CTAG_A Cuts at: 6781 6781 Size: 9445 XhoI C'TCGA_G Cuts at: 1943 3676 4187 4895 5441 1943 Size: 1733 511 708 546 5947 Fragments arranged by size: 5947 1733 708 546 511 XmaI C'CCGG_G Cuts at: 1870 4486 5399 6007 6770 1870 Size: 2616 913 608 763 4545 Fragments arranged by size: 4545 2616 913 763 608 XmnI GAAnn'nnTTC Cuts at: 2814 3539 3581 3805 7574 2814 Size: 725 42 224 3769 4685 Fragments arranged by size: 4685 3769 725 224 42 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2940 4516 7253 276 Size: 53 83 186 936 1406 1576 2737 2468 Fragments arranged by size: 2737 2468 1576 1406 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI BstZ17I EcoICRI EcoRV FspAI NruI PacI PsrI RsrII SacI SexAI SfiI XcmI