Restriction map for
pFN21AE0297
(Linear) (Six-base) MAPSORT of: KIEE0297.seq Check: 3853 from: 1 to: 4758
KIEE0297.orf
With 182 enzymes: SgfI *
October 29, 2008 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1577 3240 3620 4297 4758
Size: 1577 1663 380 677 461
Fragments arranged by size:
1663 1577 677 461 380
AatII G_ACGT'C
Cuts at: 0 10 4758
Size: 10 4748
AccI GT'mk_AC
Cuts at: 0 3461 4758
Size: 3461 1297
Acc65I G'GTAC_C
Cuts at: 0 215 4758
Size: 215 4543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 2295 4758
Size: 1579 716 2463
Fragments arranged by size:
2463 1579 716
AfeI AGC'GCT
Cuts at: 0 2956 4758
Size: 2956 1802
AflII C'TTAA_G
Cuts at: 0 472 4758
Size: 472 4286
AflIII A'CryG_T
Cuts at: 0 641 2582 4355 4758
Size: 641 1941 1773 403
Fragments arranged by size:
1941 1773 641 403
AgeI A'CCGG_T
Cuts at: 0 2180 4758
Size: 2180 2578
AleI CACnn'nnGTG
Cuts at: 0 3221 4758
Size: 3221 1537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1822 1840 2752 3548 3832 4758
Size: 1822 18 912 796 284 926
Fragments arranged by size:
1822 926 912 796 284 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3893 3925 4723 4755 4758
Size: 3893 32 798 32 3
Fragments arranged by size:
3893 798 32 32 3
AlwNI CAG_nnn'CTG
Cuts at: 0 22 897 1037 2689 3672 4176 4243 4512
Size: 22 875 140 1652 983 504 67 269
Cuts at: 4512 4758
Size: 246
Fragments arranged by size:
1652 983 875 504 269 246 140 67
22
ApaI G_GGCC'C
Cuts at: 0 257 1980 2063 2336 3146 3355 3786 4317
Size: 257 1723 83 273 810 209 431 531
Cuts at: 4317 4758
Size: 441
Fragments arranged by size:
1723 810 531 441 431 273 257 209
83
ApaLI G'TGCA_C
Cuts at: 0 247 397 1474 1512 1807 4758
Size: 247 150 1077 38 295 2951
Fragments arranged by size:
2951 1077 295 247 150 38
AvaI C'yCGr_G
Cuts at: 0 81 1972 2062 2283 2595 2767 3036 3145
Size: 81 1891 90 221 312 172 269 109
Cuts at: 3145 3303 3549 4123 4387 4563 4575 4758
Size: 158 246 574 264 176 12 183
Fragments arranged by size:
1891 574 312 269 264 246 221 183
176 172 158 109 90 81 12
BamHI G'GATC_C
Cuts at: 0 53 4758
Size: 53 4705
BanI G'GyrC_C
Cuts at: 0 215 508 835 2524 3064 3241 3396 3454
Size: 215 293 327 1689 540 177 155 58
Cuts at: 3454 3842 3864 4278 4758
Size: 388 22 414 480
Fragments arranged by size:
1689 540 480 414 388 327 293 215
177 155 58 22
BanII G_rGCy'C
Cuts at: 0 257 1951 1980 2003 2063 2336 2551 3146
Size: 257 1694 29 23 60 273 215 595
Cuts at: 3146 3355 3451 3633 3727 3766 3786 4061 4130
Size: 209 96 182 94 39 20 275 69
Cuts at: 4130 4317 4591 4708 4758
Size: 187 274 117 50
Fragments arranged by size:
1694 595 275 274 273 257 215 209
187 182 117 96 94 69 60 50
39 29 23 20
BbeI G_GCGC'C
Cuts at: 0 2528 3068 3400 4758
Size: 2528 540 332 1358
Fragments arranged by size:
2528 1358 540 332
BbsI GAAGACnn'nnnn_
Cuts at: 0 1331 1567 4758
Size: 1331 236 3191
Fragments arranged by size:
3191 1331 236
BbvCI CC'TCA_GC
Cuts at: 0 15 265 442 841 1289 2118 3848 4758
Size: 15 250 177 399 448 829 1730 910
Fragments arranged by size:
1730 910 829 448 399 250 177 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 177 211 1645 1679 4758
Size: 177 34 1434 34 3079
Fragments arranged by size:
3079 1434 177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3266 4758
Size: 3266 1492
BglI GCCn_nnn'nGGC
Cuts at: 0 2340 2902 3121 3386 3587 4004 4046 4139
Size: 2340 562 219 265 201 417 42 93
Cuts at: 4139 4758
Size: 619
Fragments arranged by size:
2340 619 562 417 265 219 201 93
42
BglII A'GATC_T
Cuts at: 0 1257 4758
Size: 1257 3501
BlpI GC'TnA_GC
Cuts at: 0 798 1021 2395 3760 4758
Size: 798 223 1374 1365 998
Fragments arranged by size:
1374 1365 998 798 223
Bme1580I G_kGCm'C
Cuts at: 0 251 257 401 840 1218 1478 1516 1811
Size: 251 6 144 439 378 260 38 295
Cuts at: 1811 1980 2022 2063 2336 3146 3244 3355 3786
Size: 169 42 41 273 810 98 111 431
Cuts at: 3786 3845 4250 4317 4758
Size: 59 405 67 441
Fragments arranged by size:
810 441 439 431 405 378 295 273
260 251 169 144 111 98 67 59
42 41 38 6
BmgBI CAC'GTC
Cuts at: 0 642 1782 1806 1926 3195 4758
Size: 642 1140 24 120 1269 1563
Fragments arranged by size:
1563 1269 1140 642 120 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1329 1974 2812 3943 4758
Size: 1329 645 838 1131 815
Fragments arranged by size:
1329 1131 838 815 645
BmtI G_CTAG'C
Cuts at: 0 3581 4758
Size: 3581 1177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1545 3465 4758
Size: 1545 1920 1293
Fragments arranged by size:
1920 1545 1293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 488 639 1570 2134 2475 2487 2743
Size: 79 409 151 931 564 341 12 256
Cuts at: 2743 2867 3387 3950 4399 4534 4758
Size: 124 520 563 449 135 224
Fragments arranged by size:
931 564 563 520 449 409 341 256
224 151 135 124 79 12
Bpu10I CC'TnA_GC
Cuts at: 0 15 265 442 679 841 1289 2118 3848
Size: 15 250 177 237 162 448 829 1730
Cuts at: 3848 4758
Size: 910
Fragments arranged by size:
1730 910 829 448 250 237 177 162
15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 2179 2194 2203 4758
Size: 1234 945 15 9 2555
Fragments arranged by size:
2555 1234 945 15 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1300 2069 4758
Size: 1300 769 2689
Fragments arranged by size:
2689 1300 769
BsaAI yAC'GTr
Cuts at: 0 363 4159 4758
Size: 363 3796 599
Fragments arranged by size:
3796 599 363
BsaBI GATnn'nnATC
Cuts at: 0 4556 4758
Size: 4556 202
BsaHI Gr'CG_yC
Cuts at: 0 7 670 719 2525 3065 3397 4758
Size: 7 663 49 1806 540 332 1361
Fragments arranged by size:
1806 1361 663 540 332 49 7
BsaWI w'CCGG_w
Cuts at: 0 1742 2180 4758
Size: 1742 438 2578
Fragments arranged by size:
2578 1742 438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 599 629 1396 1426 2533 2557 2563 2587
Size: 599 30 767 30 1107 24 6 24
Cuts at: 2587 4594 4624 4690 4720 4723 4753 4758
Size: 2007 30 66 30 3 30 5
Fragments arranged by size:
2007 1107 767 599 66 30 30 30
30 30 24 24 6 5 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1997 2457 4588 4611 4744 4758
Size: 1997 460 2131 23 133 14
Fragments arranged by size:
2131 1997 460 133 23 14
BseYI C'CCAG_C
Cuts at: 0 846 974 1016 1732 3298 3369 3755 3766
Size: 846 128 42 716 1566 71 386 11
Cuts at: 3766 4021 4129 4286 4412 4454 4505 4591 4758
Size: 255 108 157 126 42 51 86 167
Fragments arranged by size:
1566 846 716 386 255 167 157 128
126 108 86 71 51 42 42 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1314 1413 1695 1906 2634 2971 3220 3295
Size: 1314 99 282 211 728 337 249 75
Cuts at: 3295 3451 3541 4126 4186 4758
Size: 156 90 585 60 572
Fragments arranged by size:
1314 728 585 572 337 282 249 211
156 99 90 75 60
BsiEI CG_ry'CG
Cuts at: 0 1082 1156 2532 3082 4758
Size: 1082 74 1376 550 1676
Fragments arranged by size:
1676 1376 1082 550 74
BsiHKAI G_wGCw'C
Cuts at: 0 251 383 401 470 1385 1478 1516 1610
Size: 251 132 18 69 915 93 38 94
Cuts at: 1610 1811 1882 1951 2551 3043 3451 4584 4708
Size: 201 71 69 600 492 408 1133 124
Cuts at: 4708 4758
Size: 50
Fragments arranged by size:
1133 915 600 492 408 251 201 132
124 94 93 71 69 69 50 38
18
BsiWI C'GTAC_G
Cuts at: 0 359 4758
Size: 359 4399
Bsp1286I G_dGCh'C
Cuts at: 0 251 257 383 401 470 840 1218 1385
Size: 251 6 126 18 69 370 378 167
Cuts at: 1385 1478 1516 1610 1811 1882 1951 1980 2003
Size: 93 38 94 201 71 69 29 23
Cuts at: 2003 2022 2063 2336 2551 3043 3146 3244 3355
Size: 19 41 273 215 492 103 98 111
Cuts at: 3355 3451 3633 3727 3766 3786 3845 4061 4130
Size: 96 182 94 39 20 59 216 69
Cuts at: 4130 4250 4317 4584 4591 4708 4758
Size: 120 67 267 7 117 50
Fragments arranged by size:
492 378 370 273 267 251 216 215
201 182 167 126 120 117 111 103
98 96 94 94 93 71 69 69
69 67 59 50 41 39 38 29
23 20 19 18 7 6
BspEI T'CCGG_A
Cuts at: 0 1742 4758
Size: 1742 3016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 740 1577 3240 3620 4297 4758
Size: 584 156 837 1663 380 677 461
Fragments arranged by size:
1663 837 677 584 461 380 156
BsrBI CCG'CTC
Cuts at: 0 590 2465 2501 4758
Size: 590 1875 36 2257
Fragments arranged by size:
2257 1875 590 36
BsrDI GCAATG_nn'
Cuts at: 0 1806 4758
Size: 1806 2952
BsrFI r'CCGG_y
Cuts at: 0 1155 1852 2180 2527 2646 2785 4758
Size: 1155 697 328 347 119 139 1973
Fragments arranged by size:
1973 1155 697 347 328 139 119
BssSI C'ACGA_G
Cuts at: 0 544 1609 2875 4758
Size: 544 1065 1266 1883
Fragments arranged by size:
1883 1266 1065 544
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1362 1464 3672 4758
Size: 162 1200 102 2208 1086
Fragments arranged by size:
2208 1200 1086 162 102
BstEII G'GTnAC_C
Cuts at: 0 2385 4758
Size: 2385 2373
BstXI CCAn_nnnn'nTGG
Cuts at: 0 969 1192 3703 3882 4497 4758
Size: 969 223 2511 179 615 261
Fragments arranged by size:
2511 969 615 261 223 179
BstYI r'GATC_y
Cuts at: 0 53 1257 1700 4349 4758
Size: 53 1204 443 2649 409
Fragments arranged by size:
2649 1204 443 409 53
Bsu36I CC'TnA_GG
Cuts at: 0 1142 3500 4758
Size: 1142 2358 1258
Fragments arranged by size:
2358 1258 1142
BtgI C'CryG_G
Cuts at: 0 240 635 661 878 983 1389 1764 1907
Size: 240 395 26 217 105 406 375 143
Cuts at: 1907 2336 2356 2576 2809 2841 2944 3060 3116
Size: 429 20 220 233 32 103 116 56
Cuts at: 3116 3260 4149 4205 4758
Size: 144 889 56 553
Fragments arranged by size:
889 553 429 406 395 375 240 233
220 217 144 143 116 105 103 56
56 32 26 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3121 4273 4758
Size: 3121 1152 485
Fragments arranged by size:
3121 1152 485
BtsI GCAGTG_nn'
Cuts at: 0 20 1077 2022 2325 3439 4758
Size: 20 1057 945 303 1114 1319
Fragments arranged by size:
1319 1114 1057 945 303 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2016 2051 4758
Size: 2016 35 2707
Fragments arranged by size:
2707 2016 35
DraIII CAC_nnn'GTG
Cuts at: 0 246 2976 4758
Size: 246 2730 1782
Fragments arranged by size:
2730 1782 246
EaeI y'GGCC_r
Cuts at: 0 803 1079 1153 2529 2838 2844 3079 3315
Size: 803 276 74 1376 309 6 235 236
Cuts at: 3315 3470 3540 3741 4140 4344 4758
Size: 155 70 201 399 204 414
Fragments arranged by size:
1376 803 414 399 309 276 236 235
204 201 155 74 70 6
EagI C'GGCC_G
Cuts at: 0 1079 1153 2529 3079 4758
Size: 1079 74 1376 550 1679
Fragments arranged by size:
1679 1376 1079 550 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2906 3010 3348 4758
Size: 2906 104 338 1410
Fragments arranged by size:
2906 1410 338 104
EcoICRI GAG'CTC
Cuts at: 0 1949 2549 3449 4706 4758
Size: 1949 600 900 1257 52
Fragments arranged by size:
1949 1257 900 600 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 488 639 1570 1579 2134 2295 2475
Size: 79 409 151 931 9 555 161 180
Cuts at: 2475 2487 2743 2867 3387 3950 4399 4534 4758
Size: 12 256 124 520 563 449 135 224
Fragments arranged by size:
931 563 555 520 449 409 256 224
180 161 151 135 124 79 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 503 1228 4185 4726 4758
Size: 503 725 2957 541 32
Fragments arranged by size:
2957 725 541 503 32
EcoO109I rG'GnC_Cy
Cuts at: 0 254 438 573 1146 1976 2059 3142 3352
Size: 254 184 135 573 830 83 1083 210
Cuts at: 3352 3588 3782 3783 3909 4017 4313 4314 4518
Size: 236 194 1 126 108 296 1 204
Cuts at: 4518 4758
Size: 240
Fragments arranged by size:
1083 830 573 296 254 240 236 210
204 194 184 135 126 108 83 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3552 4758
Size: 3552 1206
FseI GG_CCGG'CC
Cuts at: 0 1159 4758
Size: 1159 3599
FspI TGC'GCA
Cuts at: 0 2158 4758
Size: 2158 2600
HaeII r_GCGC'y
Cuts at: 0 599 697 1174 2415 2528 2595 2755 2958
Size: 599 98 477 1241 113 67 160 203
Cuts at: 2958 3068 3230 3400 4758
Size: 110 162 170 1358
Fragments arranged by size:
1358 1241 599 477 203 170 162 160
113 110 98 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1720 1752 2215 2247 2557 2589 2920 2952
Size: 1720 32 463 32 310 32 331 32
Cuts at: 2952 3873 3905 4690 4722 4758
Size: 921 32 785 32 36
Fragments arranged by size:
1720 921 785 463 331 310 36 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1098 4479 4758
Size: 1098 3381 279
Fragments arranged by size:
3381 1098 279
KasI G'GCGC_C
Cuts at: 0 2524 3064 3396 4758
Size: 2524 540 332 1362
Fragments arranged by size:
2524 1362 540 332
KpnI G_GTAC'C
Cuts at: 0 219 4758
Size: 219 4539
MluI A'CGCG_T
Cuts at: 0 2582 4758
Size: 2582 2176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1658 1700 2345 3368 4758
Size: 1658 42 645 1023 1390
Fragments arranged by size:
1658 1390 1023 645 42
MscI TGG'CCA
Cuts at: 0 2840 3472 3542 3743 4758
Size: 2840 632 70 201 1015
Fragments arranged by size:
2840 1015 632 201 70
MslI CAynn'nnrTG
Cuts at: 0 1209 1468 1599 3221 3427 3880 4758
Size: 1209 259 131 1622 206 453 878
Fragments arranged by size:
1622 1209 878 453 259 206 131
MspA1I CmG'CkG
Cuts at: 0 67 118 130 269 451 663 738 802
Size: 67 51 12 139 182 212 75 64
Cuts at: 802 809 832 1239 1359 1418 1824 2140 2154
Size: 7 23 407 120 59 406 316 14
Cuts at: 2154 2338 2358 2403 2554 2618 2712 2811 2946
Size: 184 20 45 151 64 94 99 135
Cuts at: 2946 2991 3062 3626 3672 3759 4176 4758
Size: 45 71 564 46 87 417 582
Fragments arranged by size:
582 564 417 407 406 316 212 184
182 151 139 135 120 99 94 87
75 71 67 64 64 59 51 46
45 45 23 20 14 12 7
NaeI GCC'GGC
Cuts at: 0 1157 1854 2529 2648 2787 4758
Size: 1157 697 675 119 139 1971
Fragments arranged by size:
1971 1157 697 675 139 119
NarI GG'CG_CC
Cuts at: 0 2525 3065 3397 4758
Size: 2525 540 332 1361
Fragments arranged by size:
2525 1361 540 332
NcoI C'CATG_G
Cuts at: 0 983 4205 4758
Size: 983 3222 553
Fragments arranged by size:
3222 983 553
NdeI CA'TA_TG
Cuts at: 0 1596 4758
Size: 1596 3162
NgoMIV G'CCGG_C
Cuts at: 0 1155 1852 2527 2646 2785 4758
Size: 1155 697 675 119 139 1973
Fragments arranged by size:
1973 1155 697 675 139 119
NheI G'CTAG_C
Cuts at: 0 3577 4758
Size: 3577 1181
NspI r_CATG'y
Cuts at: 0 198 1467 1482 1753 3426 3666 3819 4332
Size: 198 1269 15 271 1673 240 153 513
Cuts at: 4332 4359 4758
Size: 27 399
Fragments arranged by size:
1673 1269 513 399 271 240 198 153
27 15
PasI CC'CwG_GG
Cuts at: 0 328 2104 2520 3583 3639 3650 4758
Size: 328 1776 416 1063 56 11 1108
Fragments arranged by size:
1776 1108 1063 416 328 56 11
PciI A'CATG_T
Cuts at: 0 4355 4758
Size: 4355 403
PflMI CCAn_nnn'nTGG
Cuts at: 0 1908 3981 4206 4512 4758
Size: 1908 2073 225 306 246
Fragments arranged by size:
2073 1908 306 246 225
PfoI T'CCnGG_A
Cuts at: 0 56 2300 4511 4758
Size: 56 2244 2211 247
Fragments arranged by size:
2244 2211 247 56
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4723 4755 4758
Size: 4723 32 3
Fragments arranged by size:
4723 32 3
PpuMI rG'GwC_Cy
Cuts at: 0 573 3909 4017 4758
Size: 573 3336 108 741
Fragments arranged by size:
3336 741 573 108
PspOMI G'GGCC_C
Cuts at: 0 253 1976 2059 2332 3142 3351 3782 4313
Size: 253 1723 83 273 810 209 431 531
Cuts at: 4313 4758
Size: 445
Fragments arranged by size:
1723 810 531 445 431 273 253 209
83
PspXI vC'TCGA_Gb
Cuts at: 0 2595 4123 4563 4758
Size: 2595 1528 440 195
Fragments arranged by size:
2595 1528 440 195
PstI C_TGCA'G
Cuts at: 0 117 432 737 1993 2408 3029 3617 3811
Size: 117 315 305 1256 415 621 588 194
Cuts at: 3811 4758
Size: 947
Fragments arranged by size:
1256 947 621 588 415 315 305 194
117
PvuII CAG'CTG
Cuts at: 0 1239 1359 2154 2403 3672 3759 4176 4758
Size: 1239 120 795 249 1269 87 417 582
Fragments arranged by size:
1269 1239 795 582 417 249 120 87
RsrII CG'GwC_CG
Cuts at: 0 1644 4758
Size: 1644 3114
SacI G_AGCT'C
Cuts at: 0 1951 2551 3451 4708 4758
Size: 1951 600 900 1257 50
Fragments arranged by size:
1951 1257 900 600 50
SacII CC_GC'GG
Cuts at: 0 664 2339 2359 2812 2947 3063 4758
Size: 664 1675 20 453 135 116 1695
Fragments arranged by size:
1695 1675 664 453 135 116 20
SanDI GG'GwC_CC
Cuts at: 0 3909 4758
Size: 3909 849
SexAI A'CCwGG_T
Cuts at: 0 3176 4013 4481 4758
Size: 3176 837 468 277
Fragments arranged by size:
3176 837 468 277
SfcI C'TryA_G
Cuts at: 0 113 428 733 1989 2404 3025 3555 3571
Size: 113 315 305 1256 415 621 530 16
Cuts at: 3571 3613 3807 4085 4758
Size: 42 194 278 673
Fragments arranged by size:
1256 673 621 530 415 315 305 278
194 113 42 16
SfoI GGC'GCC
Cuts at: 0 2526 3066 3398 4758
Size: 2526 540 332 1360
Fragments arranged by size:
2526 1360 540 332
SgrAI Cr'CCGG_yG
Cuts at: 0 2646 4758
Size: 2646 2112
SmaI CCC'GGG
Cuts at: 0 83 1974 2064 2285 3305 3551 4758
Size: 83 1891 90 221 1020 246 1207
Fragments arranged by size:
1891 1207 1020 246 221 90 83
SmlI C'TyrA_G
Cuts at: 0 472 1249 2194 2209 2218 2595 4123 4563
Size: 472 777 945 15 9 377 1528 440
Cuts at: 4563 4758
Size: 195
Fragments arranged by size:
1528 945 777 472 440 377 195 15
9
SphI G_CATG'C
Cuts at: 0 4332 4758
Size: 4332 426
StuI AGG'CCT
Cuts at: 0 94 1426 1537 2512 3017 3569 4376 4432
Size: 94 1332 111 975 505 552 807 56
Cuts at: 4432 4758
Size: 326
Fragments arranged by size:
1332 975 807 552 505 326 111 94
56
StyI C'CwwG_G
Cuts at: 0 141 983 1334 4205 4250 4758
Size: 141 842 351 2871 45 508
Fragments arranged by size:
2871 842 508 351 141 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 975 1030 1150 1199 1363 1684 1957
Size: 771 204 55 120 49 164 321 273
Cuts at: 1957 2983 3962 4758
Size: 1026 979 796
Fragments arranged by size:
1026 979 796 771 321 273 204 164
120 55 49
TatI w'GTAC_w
Cuts at: 0 1460 2657 3203 4758
Size: 1460 1197 546 1555
Fragments arranged by size:
1555 1460 1197 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 599 631 932 950 964 982 1396 1428
Size: 599 32 301 18 14 18 414 32
Cuts at: 1428 1558 1590 4594 4626 4758
Size: 130 32 3004 32 132
Fragments arranged by size:
3004 599 414 301 132 130 32 32
32 32 18 18 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 876 1905 3228 3388 3736 3901 3921
Size: 765 111 1029 1323 160 348 165 20
Cuts at: 3921 4257 4758
Size: 336 501
Fragments arranged by size:
1323 1029 765 501 348 336 165 160
111 20
XhoI C'TCGA_G
Cuts at: 0 2595 4123 4563 4758
Size: 2595 1528 440 195
Fragments arranged by size:
2595 1528 440 195
XmaI C'CCGG_G
Cuts at: 0 81 1972 2062 2283 3303 3549 4758
Size: 81 1891 90 221 1020 246 1209
Fragments arranged by size:
1891 1209 1020 246 221 90 81
XmnI GAAnn'nnTTC
Cuts at: 0 1447 4758
Size: 1447 3311
ZraI GAC'GTC
Cuts at: 0 8 4758
Size: 8 4750
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PasI PciI PflMI PfoI PpiI
PpuMI PspOMI PspXI PstI PvuII RsrII SacI SacII
SanDI SexAI SfcI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AhdI ApoI AscI AseI AsiSI AvrII BaeI
BclI BfrBI BsmI BsmBI BspHI BsrGI BssHII BstBI
BstZ17I ClaI DraI DrdI EarI EcoRI EcoRV FspAI
HindIII HpaI MfeI NotI NruI NsiI PacI PmeI
PmlI PshAI PsiI PsrI PvuI SalI SapI SbfI
ScaI SfiI SgfI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI