Restriction map for
pFN21AB6017
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BcgI BciVI BclI BfrI
BlnI BsaBI BseAI BsiWI BspEI
BspHI BspLU11I BsrBI BssHII DraI
Eam1105I EarI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
Ksp632I KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SacII SalI SapI SbfI
ScaI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI TfiI Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 779 GGTACC G^GTACC
AccI 1435 GTATAC GT^[AC][GT]AC
AflIII 2423 ACGTGT A^C[GA][CT]GT
Alw44I 1444 GTGCAC G^TGCAC
AocI 806 CCTCAGG CC^T[ACGT]AGG
Aor51HI 397 AGCGCT AGC^GCT
ApaI 1364 GGGCCC GGGCC^C
ApaLI 1444 GTGCAC G^TGCAC
Asp718I 779 GGTACC G^GTACC
BamHI 1070 GGATCC G^GATCC
BbeI 431 GGCGCC GGCGC^C
BbvCI 928 GCTGAGG CCTCAGC(-5/-2)
BglII 572 AGATCT A^GATCT
BsmBI 357 CGTCTC CGTCTC(1/5)
BsmI 1383 GAATGC GAATGC(1/-1)
Bsp1407I 2057 TGTACA T^GTACA
BspMI 2120 ACCTGC ACCTGC(4/8)
BsrDI 1893 CATTGC GCAATG(2/0)
BsrGI 2057 TGTACA T^GTACA
Bst1107I 1435 GTATAC GTA^TAC
BstAPI 2337 GCACCGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1456 TTCGAA TT^CGAA
BstEII 1146 GGTCACC G^GT[ACGT]ACC
BstPI 1146 GGTCACC G^GT[ACGT]ACC
BstZ17I 1435 GTATAC GTA^TAC
Bsu36I 806 CCTCAGG CC^T[ACGT]AGG
CpoI 1408 CGGACCG CG^G[AT]CCG
DraIII 1498 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1948 GACTTAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1388 CGGCCG C^GGCCG
EclXI 1388 CGGCCG C^GGCCG
Eco47III 397 AGCGCT AGC^GCT
Eco52I 1388 CGGCCG C^GGCCG
Eco81I 806 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1146 GGTCACC G^GT[ACGT]ACC
HincII 1957 GTCAAC GT[CT]^[GA]AC
HindII 1957 GTCAAC GT[CT]^[GA]AC
KasI 431 GGCGCC G^GCGCC
KpnI 779 GGTACC GGTAC^C
MseI 1951 TTAA T^TAA
NarI 431 GGCGCC GG^CGCC
NspI 2342 GCATGC [GA]CATG^[CT]
NspV 1456 TTCGAA TT^CGAA
PflMI 2186 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2103 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1408 CGGACCG CG^G[AT]CCG
SfiI 804 GGCCTCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 431 GGCGCC GGC^GCC
SfuI 1456 TTCGAA TT^CGAA
SphI 2342 GCATGC GCATG^C
SspBI 2057 TGTACA T^GTACA
Tru9I 1951 TTAA T^TAA
Van91I 2186 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwI 1071 GATCC GGATC(4/5)
1070 GGATC
AlwNI 188 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
994 CAGAGGCTG
BalI 1346 TGGCCA TGG^CCA
1877 TGGCCA
BbrPI 1033 CACGTG CAC^GTG
2443 CACGTG
BfaI 622 CTAG C^TAG
1465 CTAG
BlpI 351 GCTGAGC GC^T[ACGT]AGC
2364 GCTGAGC
Bpu1102I 351 GCTGAGC GC^T[ACGT]AGC
2364 GCTGAGC
BsaI 1849 GAGACC GGTCTC(1/5)
40 GGTCTC
BsaWI 1067 ACCGGA [AT]^CCGG[AT]
2153 ACCGGA
BsiEI 1065 CGACCG CG[GA][CT]^CG
1388 CGGCCG
BspDI 634 ATCGAT AT^CGAT
706 ATCGAT
BssSI 32 CTCGTG CACGAG(-5/-1)
313 CTCGTG
BstXI 1254 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2105 CCAATGTCCTGG
BstYI 572 AGATCT [GA]^GATC[CT]
1070 GGATCC
CelII 351 GCTGAGC GC^T[ACGT]AGC
2364 GCTGAGC
ClaI 634 ATCGAT AT^CGAT
706 ATCGAT
MaeI 622 CTAG C^TAG
1465 CTAG
MflI 572 AGATCT [GA]^GATC[CT]
1070 GGATCC
MluNI 1346 TGGCCA TGG^CCA
1877 TGGCCA
MscI 1346 TGGCCA TGG^CCA
1877 TGGCCA
PmaCI 1033 CACGTG CAC^GTG
2443 CACGTG
PmlI 1033 CACGTG CAC^GTG
2443 CACGTG
SacI 1687 GAGCTC GAGCT^C
2311 GAGCTC
SexAI 452 ACCTGGT A^CC[AT]GGT
983 ACCTGGT
SmlI 69 CTTGAG C^T[CT][GA]AG
2314 CTCAAG
XhoII 572 AGATCT [GA]^GATC[CT]
1070 GGATCC
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI