(Circular) (Six-base) MAPSORT of: pFN21AB6017.seq Check: 768 from: 1 to: 7186 pFN21AB6017 7186 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4134 4134 Size: 7186 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4996 278 Size: 53 83 186 936 3460 2468 Fragments arranged by size: 3460 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3441 4529 1937 Size: 1504 1088 4594 Fragments arranged by size: 4594 1504 1088 Acc65I G'GTAC_C Cuts at: 2784 4507 2784 Size: 1723 5463 AclI AA'CG_TT Cuts at: 1563 5313 5686 1563 Size: 3750 373 3063 Fragments arranged by size: 3750 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3739 4285 4301 4409 5249 6633 6901 1632 Size: 2107 546 16 108 840 1384 268 1917 Fragments arranged by size: 2107 1917 1384 840 546 268 108 16 AfeI AGC'GCT Cuts at: 2404 2404 Size: 7186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6989 AflIII A'CryG_T Cuts at: 1203 4428 6085 7062 1203 Size: 3225 1657 977 1327 Fragments arranged by size: 3225 1657 1327 977 AgeI A'CCGG_T Cuts at: 6017 6017 Size: 7186 AhdI GACnn_n'nnGTC Cuts at: 5915 5915 Size: 7186 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7186 AlwNI CAG_nnn'CTG Cuts at: 2198 3004 4929 6501 2198 Size: 806 1925 1572 2883 Fragments arranged by size: 2883 1925 1572 806 ApaI G_GGCC'C Cuts at: 3373 3373 Size: 7186 ApaLI G'TGCA_C Cuts at: 3449 5243 6399 3449 Size: 1794 1156 4236 Fragments arranged by size: 4236 1794 1156 ApoI r'AATT_y Cuts at: 1493 4495 4721 4873 6982 1493 Size: 3002 226 152 2109 1697 Fragments arranged by size: 3002 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5740 160 Size: 872 4708 1606 Fragments arranged by size: 4708 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7186 AvaI C'yCGr_G Cuts at: 1870 1943 2837 2849 3160 3823 4511 1870 Size: 73 894 12 311 663 688 4545 Fragments arranged by size: 4545 894 688 663 311 73 12 BamHI G'GATC_C Cuts at: 3075 4516 4883 3075 Size: 1441 367 5378 Fragments arranged by size: 5378 1441 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 2279 2436 2516 2657 Size: 325 217 627 417 75 157 80 141 Cuts at: 2657 2784 2939 3746 4507 5962 618 Size: 127 155 807 761 1455 1842 Fragments arranged by size: 1842 1455 807 761 627 417 325 217 157 155 141 127 80 75 BanII G_rGCy'C Cuts at: 1391 2087 2636 3373 3696 3767 4320 4505 1391 Size: 696 549 737 323 71 553 185 4072 Fragments arranged by size: 4072 737 696 553 549 323 185 71 BbeI G_GCGC'C Cuts at: 2440 2440 Size: 7186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2281 3919 3998 4087 928 Size: 971 382 1638 79 89 4027 Fragments arranged by size: 4027 1638 971 382 89 79 BbvCI CC'TCA_GC Cuts at: 2934 2934 Size: 7186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5377 5411 1903 Size: 34 3440 34 3678 Fragments arranged by size: 3678 3440 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5078 6288 5078 Size: 1210 5976 BclI T'GATC_A Cuts at: 1548 1548 Size: 7186 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2631 2816 3377 Size: 107 122 71 631 605 959 185 561 Cuts at: 3377 5797 136 Size: 2420 1525 Fragments arranged by size: 2420 1525 959 631 605 561 185 122 107 71 BglII A'GATC_T Cuts at: 2577 7181 2577 Size: 4604 2582 BlpI GC'TnA_GC Cuts at: 2357 4370 4603 2357 Size: 2013 233 4940 Fragments arranged by size: 4940 2013 233 Bme1580I G_kGCm'C Cuts at: 1790 3373 3453 5247 6403 1790 Size: 1583 80 1794 1156 2573 Fragments arranged by size: 2573 1794 1583 1156 80 BmgBI CAC'GTC Cuts at: 1318 3508 1318 Size: 2190 4996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4917 5875 442 Size: 416 53 83 399 24 3500 958 1753 Fragments arranged by size: 3500 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2573 3596 4463 5846 1239 Size: 1334 1023 867 1383 2579 Fragments arranged by size: 2579 1383 1334 1023 867 Bpu10I CC'TnA_GC Cuts at: 2934 3080 4466 2934 Size: 146 1386 5654 Fragments arranged by size: 5654 1386 146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2095 4304 5311 6176 6474 2095 Size: 2209 1007 865 298 2807 Fragments arranged by size: 2807 2209 1007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 3848 5849 882 Size: 617 552 1797 2001 2219 Fragments arranged by size: 2219 2001 1797 617 552 BsaAI yAC'GTr Cuts at: 493 1204 2980 3040 3445 4429 4450 4977 493 Size: 711 1776 60 405 984 21 527 2702 Fragments arranged by size: 2702 1776 984 711 527 405 60 21 BsaBI GATnn'nnATC Cuts at: 7180 7180 Size: 7186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2437 3056 3915 4092 Size: 53 83 186 936 904 619 859 177 Cuts at: 4092 4993 5375 6010 275 Size: 901 382 635 1451 Fragments arranged by size: 1451 936 904 901 859 635 619 382 186 177 83 53 BsaWI w'CCGG_w Cuts at: 3072 4158 5619 6017 6291 6438 6849 3072 Size: 1086 1461 398 274 147 411 3409 Fragments arranged by size: 3409 1461 1086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4046 4076 4349 4379 1178 Size: 30 165 30 2643 30 273 30 3985 Fragments arranged by size: 3985 2643 273 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 3296 3704 1186 Size: 1384 726 408 4668 Fragments arranged by size: 4668 1384 726 408 BseYI C'CCAG_C Cuts at: 2088 2120 2224 2380 2859 2884 3004 3031 3258 Size: 32 104 156 479 25 120 27 227 Cuts at: 3258 3541 3718 4177 6389 6885 2088 Size: 283 177 459 2212 496 2389 Fragments arranged by size: 2389 2212 496 479 459 283 227 177 156 120 104 32 27 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 3062 3221 3239 1163 Size: 573 1051 275 159 18 5110 Fragments arranged by size: 5110 1051 573 275 159 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3396 4636 5397 6425 1540 Size: 275 186 1072 323 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1072 1028 761 323 275 186 BsiHKAI G_wGCw'C Cuts at: 3453 3696 3975 4320 4344 4449 5247 5332 6403 Size: 243 279 345 24 105 798 85 1071 Cuts at: 6403 3453 Size: 4236 Fragments arranged by size: 4236 1071 798 345 279 243 105 85 24 BsmI GAATG_Cn' Cuts at: 3394 4697 4790 3394 Size: 1303 93 5790 Fragments arranged by size: 5790 1303 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2368 2368 Size: 7186 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2636 3373 3453 3696 3767 3975 Size: 399 297 549 737 80 243 71 208 Cuts at: 3975 4320 4344 4449 4505 5247 5332 6403 1391 Size: 345 24 105 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 737 549 399 345 297 243 208 105 85 80 71 56 24 BspEI T'CCGG_A Cuts at: 6849 6849 Size: 7186 BspHI T'CATG_A Cuts at: 5075 6805 5075 Size: 1730 5456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4134 4541 844 Size: 3290 407 3489 Fragments arranged by size: 3489 3290 407 BsrBI CCG'CTC Cuts at: 1394 1823 5073 1394 Size: 429 3250 3507 Fragments arranged by size: 3507 3250 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3897 5681 5855 65 Size: 1183 2649 1784 174 1396 Fragments arranged by size: 2649 1784 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2772 3139 3416 3512 5830 6017 1700 Size: 110 962 367 277 96 2318 187 2869 Fragments arranged by size: 2869 2318 962 367 277 187 110 96 BsrGI T'GTAC_A Cuts at: 96 4062 96 Size: 3966 3220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7186 BssSI C'ACGA_G Cuts at: 2037 2318 5246 6258 2037 Size: 281 2928 1012 2965 Fragments arranged by size: 2965 2928 1012 281 BstAPI GCAn_nnn'nTGC Cuts at: 794 4348 794 Size: 3554 3632 BstBI TT'CG_AA Cuts at: 3462 5990 3462 Size: 2528 4658 BstEII G'GTnAC_C Cuts at: 1184 3151 1184 Size: 1967 5219 BstXI CCAn_nnnn'nTGG Cuts at: 3266 4117 3266 Size: 851 6335 BstYI r'GATC_y Cuts at: 1972 2577 3075 4516 4883 5268 5285 6737 6823 Size: 605 498 1441 367 385 17 1452 86 Cuts at: 6823 6835 7181 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1441 605 498 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3442 3442 Size: 7186 Bsu36I CC'TnA_GG Cuts at: 2812 2812 Size: 7186 BtgI C'CryG_G Cuts at: 513 1065 2902 3228 3265 3427 3789 3858 4050 Size: 552 1837 326 37 162 362 69 192 Cuts at: 4050 4352 513 Size: 302 3347 Fragments arranged by size: 3347 1837 552 362 326 302 192 162 69 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2602 2914 487 Size: 683 56 1376 312 4759 Fragments arranged by size: 4759 1376 683 312 56 BtsI GCAGTG_nn' Cuts at: 2140 2727 2776 3771 4367 4702 5496 5516 2140 Size: 587 49 995 596 335 794 20 3810 Fragments arranged by size: 3810 995 794 596 587 335 49 20 ClaI AT'CG_AT Cuts at: 1554 2640 2712 1554 Size: 1086 72 6028 Fragments arranged by size: 6028 1086 72 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7151 DraI TTT'AAA Cuts at: 4490 4490 Size: 7186 DraIII CAC_nnn'GTG Cuts at: 3508 3508 Size: 7186 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3959 6193 809 Size: 507 2643 2234 1802 Fragments arranged by size: 2643 2234 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3351 3393 3510 Size: 54 1425 50 275 18 1521 42 117 Cuts at: 3510 3882 4419 4633 5522 7151 8 Size: 372 537 214 889 1629 43 Fragments arranged by size: 1629 1521 1425 889 537 372 275 214 117 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3393 4633 1537 Size: 275 1581 1240 4090 Fragments arranged by size: 4090 1581 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5116 1353 Size: 3763 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2582 5773 6147 6293 387 Size: 1064 138 993 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 993 374 146 138 EcoICRI GAG'CTC Cuts at: 3694 4318 3694 Size: 624 6562 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2573 3596 3739 4285 4301 4409 4463 Size: 393 941 1023 143 546 16 108 54 Cuts at: 4463 5249 5846 6633 6901 1239 Size: 786 597 787 268 1524 Fragments arranged by size: 1524 1023 941 787 786 597 546 393 268 143 108 54 16 EcoNI CCTnn'n_nnAGG Cuts at: 2321 2552 3473 3563 4130 2321 Size: 231 921 90 567 5377 Fragments arranged by size: 5377 921 567 231 90 EcoO109I rG'GnC_Cy Cuts at: 2113 2314 2748 2907 2986 3369 3370 3555 3934 Size: 201 434 159 79 383 1 185 379 Cuts at: 3934 4009 2113 Size: 75 5290 Fragments arranged by size: 5290 434 383 379 201 185 159 79 75 1 EcoRI G'AATT_C Cuts at: 4495 4873 4495 Size: 378 6808 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3358 4111 3358 Size: 753 6433 FspI TGC'GCA Cuts at: 5692 5692 Size: 7186 HaeII r_GCGC'y Cuts at: 1678 2406 2440 2795 3116 3685 3838 4143 6333 Size: 728 34 355 321 569 153 305 2190 Cuts at: 6333 1678 Size: 2531 Fragments arranged by size: 2531 2190 728 569 355 321 305 153 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2904 2936 5827 5859 5901 Size: 32 135 32 1332 32 2891 32 42 Cuts at: 5901 5933 1373 Size: 32 2626 Fragments arranged by size: 2891 2626 1332 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3964 4530 4776 669 Size: 988 281 2026 566 246 3079 Fragments arranged by size: 3079 2026 988 566 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7186 HpaI GTT'AAC Cuts at: 4776 4776 Size: 7186 KasI G'GCGC_C Cuts at: 2436 2436 Size: 7186 KpnI G_GTAC'C Cuts at: 2788 4511 2788 Size: 1723 5463 MfeI C'AATT_G Cuts at: 4785 4785 Size: 7186 MluI A'CGCG_T Cuts at: 7062 7062 Size: 7186 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 3792 4169 4185 6300 6484 1305 Size: 1086 1401 377 16 2115 184 2007 Fragments arranged by size: 2115 2007 1401 1086 377 184 16 MscI TGG'CCA Cuts at: 10 64 1489 1832 3353 3884 10 Size: 54 1425 343 1521 531 3312 Fragments arranged by size: 3312 1521 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2273 2663 3704 3902 4415 5144 5503 Size: 648 1107 390 1041 198 513 729 359 Cuts at: 5503 5662 518 Size: 159 2042 Fragments arranged by size: 2042 1107 1041 729 648 513 390 359 198 159 MspA1I CmG'CkG Cuts at: 1606 2120 2380 2447 3046 3574 3664 4602 5279 Size: 514 260 67 599 528 90 938 677 Cuts at: 5279 6427 6672 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 938 677 599 528 514 260 245 90 67 NaeI GCC'GGC Cuts at: 2774 3141 3514 2774 Size: 367 373 6446 Fragments arranged by size: 6446 373 367 NarI GG'CG_CC Cuts at: 2437 2437 Size: 7186 NcoI C'CATG_G Cuts at: 513 1065 3228 3427 3789 3858 513 Size: 552 2163 199 362 69 3841 Fragments arranged by size: 3841 2163 552 362 199 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 7186 NgoMIV G'CCGG_C Cuts at: 2772 3139 3512 2772 Size: 367 373 6446 Fragments arranged by size: 6446 373 367 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7186 NotI GC'GGCC_GC Cuts at: 4633 4633 Size: 7186 NspI r_CATG'y Cuts at: 1132 1229 4351 4544 6089 1132 Size: 97 3122 193 1545 2229 Fragments arranged by size: 3122 2229 1545 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7186 PciI A'CATG_T Cuts at: 6085 6085 Size: 7186 PflMI CCAn_nnn'nTGG Cuts at: 1108 4197 1108 Size: 3089 4097 PmeI GTTT'AAAC Cuts at: 4490 4490 Size: 7186 PmlI CAC'GTG Cuts at: 3040 4450 3040 Size: 1410 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5255 5287 6768 6800 5255 Size: 32 1481 32 5641 Fragments arranged by size: 5641 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2748 2907 2986 3555 3934 2748 Size: 159 79 569 379 6000 Fragments arranged by size: 6000 569 379 159 79 PshAI GACnn'nnGTC Cuts at: 1613 4112 1613 Size: 2499 4687 PsiI TTA'TAA Cuts at: 4756 4756 Size: 7186 PspOMI G'GGCC_C Cuts at: 3369 3369 Size: 7186 PstI C_TGCA'G Cuts at: 830 2147 3663 4106 4131 4538 830 Size: 1317 1516 443 25 407 3478 Fragments arranged by size: 3478 1516 1317 443 407 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7186 PvuII CAG'CTG Cuts at: 2380 3574 3664 2380 Size: 1194 90 5902 Fragments arranged by size: 5902 1194 90 RsrII CG'GwC_CG Cuts at: 3414 3414 Size: 7186 SacI G_AGCT'C Cuts at: 3696 4320 3696 Size: 624 6562 SalI G'TCGA_C Cuts at: 1936 4528 1936 Size: 2592 4594 SanDI GG'GwC_CC Cuts at: 2907 2907 Size: 7186 SbfI CC_TGCA'GG Cuts at: 4538 4538 Size: 7186 ScaI AGT'ACT Cuts at: 5434 7146 5434 Size: 1712 5474 SexAI A'CCwGG_T Cuts at: 2457 2988 2457 Size: 531 6655 SfcI C'TryA_G Cuts at: 826 1045 2143 2589 3659 4102 4127 4534 5669 Size: 219 1098 446 1070 443 25 407 1135 Cuts at: 5669 6350 6541 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1098 1070 681 446 443 407 219 191 25 SfiI GGCCn_nnn'nGGCC Cuts at: 2816 2816 Size: 7186 SfoI GGC'GCC Cuts at: 2438 2438 Size: 7186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7186 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7186 SmaI CCC'GGG Cuts at: 1872 4513 1872 Size: 2641 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2074 4319 5290 6191 6453 820 Size: 197 926 131 2245 971 901 262 1553 Fragments arranged by size: 2245 1553 971 926 901 262 197 131 SnaBI TAC'GTA Cuts at: 493 4977 493 Size: 4484 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7186 SphI G_CATG'C Cuts at: 1132 4351 4544 1132 Size: 3219 193 3774 Fragments arranged by size: 3774 3219 193 SspI AAT'ATT Cuts at: 5 52 5110 5 Size: 47 5058 2081 Fragments arranged by size: 5058 2081 47 StuI AGG'CCT Cuts at: 1517 2417 3468 3644 1517 Size: 900 1051 176 5059 Fragments arranged by size: 5059 1051 900 176 StyI C'CwwG_G Cuts at: 513 1065 2109 3210 3228 3327 3427 3474 3564 Size: 552 1044 1101 18 99 100 47 90 Cuts at: 3564 3789 3858 4625 513 Size: 225 69 767 3074 Fragments arranged by size: 3074 1101 1044 767 552 225 100 99 90 69 47 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2267 2876 3272 5213 5230 5383 5568 Size: 1255 687 609 396 1941 17 153 185 Cuts at: 5568 6013 325 Size: 445 1498 Fragments arranged by size: 1941 1498 1255 687 609 445 396 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4062 4071 5432 Size: 274 80 33 51 1443 2085 9 1361 Cuts at: 5432 7144 96 Size: 1712 138 Fragments arranged by size: 2085 1712 1443 1361 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4349 4381 7146 7178 4349 Size: 32 2765 32 4357 Fragments arranged by size: 4357 2765 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7186 XbaI T'CTAG_A Cuts at: 4522 4522 Size: 7186 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2096 3266 3361 3571 4359 2096 Size: 1170 95 210 788 4923 Fragments arranged by size: 4923 1170 788 210 95 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7186 XmaI C'CCGG_G Cuts at: 1870 4511 1870 Size: 2641 4545 XmnI GAAnn'nnTTC Cuts at: 5315 5315 Size: 7186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4994 276 Size: 53 83 186 936 3460 2468 Fragments arranged by size: 3460 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI SacII SapI SrfI SwaI