(Circular) (Six-base) MAPSORT of: pFN21AB6016.seq Check: 6280 from: 1 to: 7528 pFN21AB6016 7528 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5338 278 Size: 53 83 186 936 3802 2468 Fragments arranged by size: 3802 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4871 1937 Size: 2934 4594 Acc65I G'GTAC_C Cuts at: 2877 4849 2877 Size: 1972 5556 AclI AA'CG_TT Cuts at: 1563 2554 5655 6028 1563 Size: 991 3101 373 3063 Fragments arranged by size: 3101 3063 991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2853 4059 4416 4547 5591 6975 7243 1632 Size: 1221 1206 357 131 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1221 1206 1044 357 268 131 AflII C'TTAA_G Cuts at: 820 1017 4790 820 Size: 197 3773 3558 Fragments arranged by size: 3773 3558 197 AflIII A'CryG_T Cuts at: 1203 4247 6427 7404 1203 Size: 3044 2180 977 1327 Fragments arranged by size: 3044 2180 1327 977 AgeI A'CCGG_T Cuts at: 6359 6359 Size: 7528 AhdI GACnn_n'nnGTC Cuts at: 6257 6257 Size: 7528 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7528 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3801 1184 Size: 2617 4911 AlwNI CAG_nnn'CTG Cuts at: 2045 3707 4317 5271 6843 2045 Size: 1662 610 954 1572 2730 Fragments arranged by size: 2730 1662 1572 954 610 ApaLI G'TGCA_C Cuts at: 4009 5585 6741 4009 Size: 1576 1156 4796 Fragments arranged by size: 4796 1576 1156 ApoI r'AATT_y Cuts at: 1493 2690 2932 3188 3366 3465 3671 4155 4585 Size: 1197 242 256 178 99 206 484 430 Cuts at: 4585 4837 5063 5215 7324 1493 Size: 252 226 152 2109 1697 Fragments arranged by size: 2109 1697 1197 484 430 256 252 242 226 206 178 152 99 AseI AT'TA_AT Cuts at: 160 1032 6082 160 Size: 872 5050 1606 Fragments arranged by size: 5050 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7528 AvaI C'yCGr_G Cuts at: 1870 1943 2388 4004 4853 1870 Size: 73 445 1616 849 4545 Fragments arranged by size: 4545 1616 849 445 73 BamHI G'GATC_C Cuts at: 3060 4306 4858 5225 3060 Size: 1246 552 367 5363 Fragments arranged by size: 5363 1246 552 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2877 4669 4849 6304 Size: 325 217 627 308 782 1792 180 1455 Cuts at: 6304 618 Size: 1842 Fragments arranged by size: 1842 1792 1455 782 627 325 308 217 180 BanII G_rGCy'C Cuts at: 1391 3594 3656 4847 1391 Size: 2203 62 1191 4072 Fragments arranged by size: 4072 2203 1191 62 BbeI G_GCGC'C Cuts at: 2099 2099 Size: 7528 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2386 3999 928 Size: 971 487 1613 4457 Fragments arranged by size: 4457 1613 971 487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2049 2083 3995 4029 4334 4368 5719 Size: 34 112 34 1912 34 305 34 1351 Cuts at: 5719 5753 1903 Size: 34 3678 Fragments arranged by size: 3678 1912 1351 305 112 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5420 6630 4373 Size: 1047 1210 5271 Fragments arranged by size: 5271 1210 1047 BclI T'GATC_A Cuts at: 1548 2679 1548 Size: 1131 6397 BfrBI ATG'CAT Cuts at: 2428 4485 2428 Size: 2057 5471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2057 6139 Size: 107 122 71 631 605 343 42 4082 Cuts at: 6139 136 Size: 1525 Fragments arranged by size: 4082 1525 631 605 343 122 107 71 42 BglII A'GATC_T Cuts at: 3178 7523 3178 Size: 4345 3183 BlpI GC'TnA_GC Cuts at: 4945 4945 Size: 7528 Bme1580I G_kGCm'C Cuts at: 1790 4013 4674 5589 6745 1790 Size: 2223 661 915 1156 2573 Fragments arranged by size: 2573 2223 1156 915 661 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 6700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5259 6217 442 Size: 416 53 83 399 24 3842 958 1753 Fragments arranged by size: 3842 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2067 2477 4286 6188 1239 Size: 828 410 1809 1902 2579 Fragments arranged by size: 2579 1902 1809 828 410 Bpu10I CC'TnA_GC Cuts at: 3140 3140 Size: 7528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4429 4540 5653 6518 6816 3670 Size: 759 111 1113 865 298 4382 Fragments arranged by size: 4382 1113 865 759 298 111 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4684 6191 882 Size: 617 3185 1507 2219 Fragments arranged by size: 3185 2219 1507 617 BsaAI yAC'GTr Cuts at: 493 1204 4248 5319 493 Size: 711 3044 1071 2702 Fragments arranged by size: 3044 2702 1071 711 BsaBI GATnn'nnATC Cuts at: 3729 7522 3729 Size: 3793 3735 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2096 5335 5717 6352 Size: 53 83 186 936 563 3239 382 635 Cuts at: 6352 275 Size: 1451 Fragments arranged by size: 3239 1451 936 635 563 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3998 5961 6359 6633 6780 7191 3998 Size: 1963 398 274 147 411 4335 Fragments arranged by size: 4335 1963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2502 2532 2982 3012 1178 Size: 30 165 30 1099 30 450 30 5694 Fragments arranged by size: 5694 1099 450 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3446 1186 Size: 2260 5268 BseYI C'CCAG_C Cuts at: 4310 6731 7227 4310 Size: 2421 496 4611 Fragments arranged by size: 4611 2421 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2280 3098 3999 1163 Size: 573 544 818 901 4692 Fragments arranged by size: 4692 901 818 573 544 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2086 4978 5739 6767 1540 Size: 275 186 85 2892 761 1028 2301 Fragments arranged by size: 2892 2301 1028 761 275 186 85 BsiHKAI G_wGCw'C Cuts at: 3075 3594 3656 4013 4193 5589 5674 6745 3075 Size: 519 62 357 180 1396 85 1071 3858 Fragments arranged by size: 3858 1396 1071 519 357 180 85 62 BsiWI C'GTAC_G Cuts at: 2139 2139 Size: 7528 BsmI GAATG_Cn' Cuts at: 5039 5132 5039 Size: 93 7435 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3075 3594 3656 4013 4193 4674 4847 Size: 399 1285 519 62 357 180 481 173 Cuts at: 4847 5589 5674 6745 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1285 1071 742 519 481 399 357 180 173 85 62 BspEI T'CCGG_A Cuts at: 3998 7191 3998 Size: 3193 4335 BspHI T'CATG_A Cuts at: 2157 2646 5417 7147 2157 Size: 489 2771 1730 2538 Fragments arranged by size: 2771 2538 1730 489 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4883 844 Size: 4039 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2015 5415 1394 Size: 429 192 3400 3507 Fragments arranged by size: 3507 3400 429 192 BsrDI GCAATG_nn' Cuts at: 65 1248 6023 6197 65 Size: 1183 4775 174 1396 Fragments arranged by size: 4775 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 4693 6172 6359 1700 Size: 110 320 2563 1479 187 2869 Fragments arranged by size: 2869 2563 1479 320 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7528 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7528 BssSI C'ACGA_G Cuts at: 5588 6600 5588 Size: 1012 6516 BstAPI GCAn_nnn'nTGC Cuts at: 794 4278 794 Size: 3484 4044 BstBI TT'CG_AA Cuts at: 6332 6332 Size: 7528 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7528 BstYI r'GATC_y Cuts at: 1972 3060 3178 4306 4399 4462 4785 4858 5225 Size: 1088 118 1128 93 63 323 73 367 Cuts at: 5225 5610 5627 7079 7165 7177 7523 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1128 1088 385 367 346 323 118 93 86 73 63 17 12 Bsu36I CC'TnA_GG Cuts at: 4458 4458 Size: 7528 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2055 2191 3632 4321 513 Size: 552 939 27 24 136 1441 689 3720 Fragments arranged by size: 3720 1441 939 689 552 136 27 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6789 Fragments arranged by size: 6789 683 56 BtsI GCAGTG_nn' Cuts at: 3305 4011 4288 5044 5838 5858 3305 Size: 706 277 756 794 20 4975 Fragments arranged by size: 4975 794 756 706 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7528 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7493 DraI TTT'AAA Cuts at: 3321 4832 3321 Size: 1511 6017 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6535 809 Size: 507 5219 1802 Fragments arranged by size: 5219 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2028 2083 Size: 54 1425 50 275 18 177 21 55 Cuts at: 2083 2768 4807 4975 5864 7493 8 Size: 685 2039 168 889 1629 43 Fragments arranged by size: 2039 1629 1425 889 685 275 177 168 55 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2083 4975 1537 Size: 275 271 2892 4090 Fragments arranged by size: 4090 2892 275 271 EarI CTCTTCn'nnn_ Cuts at: 1353 3633 3819 3861 4035 5458 1353 Size: 2280 186 42 174 1423 3423 Fragments arranged by size: 3423 2280 1423 186 174 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2091 6115 6489 6635 387 Size: 1064 138 502 4024 374 146 1280 Fragments arranged by size: 4024 1280 1064 502 374 146 138 EcoICRI GAG'CTC Cuts at: 3592 3654 3592 Size: 62 7466 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2477 2853 4059 4286 4416 4547 Size: 393 435 410 376 1206 227 130 131 Cuts at: 4547 5591 6188 6975 7243 1239 Size: 1044 597 787 268 1524 Fragments arranged by size: 1524 1206 1044 787 597 435 410 393 376 268 227 131 130 EcoNI CCTnn'n_nnAGG Cuts at: 3290 3290 Size: 7528 EcoO109I rG'GnC_Cy Cuts at: 2725 3607 2725 Size: 882 6646 EcoRI G'AATT_C Cuts at: 3465 4585 4837 5215 3465 Size: 1120 252 378 5778 Fragments arranged by size: 5778 1120 378 252 EcoRV GAT'ATC Cuts at: 2173 2453 2173 Size: 280 7248 FspI TGC'GCA Cuts at: 6034 6034 Size: 7528 HaeII r_GCGC'y Cuts at: 1678 2099 6675 1678 Size: 421 4576 2531 Fragments arranged by size: 4576 2531 421 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2460 2492 2502 2534 4371 Size: 32 135 32 888 32 10 32 1837 Cuts at: 4371 4384 4403 4416 6169 6201 6243 6275 1373 Size: 13 19 13 1753 32 42 32 2626 Fragments arranged by size: 2626 1837 1753 888 135 42 32 32 32 32 32 32 19 13 13 10 HincII GTy'rAC Cuts at: 669 1657 1938 2230 4872 5118 669 Size: 988 281 292 2642 246 3079 Fragments arranged by size: 3079 2642 988 292 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7528 HpaI GTT'AAC Cuts at: 5118 5118 Size: 7528 KasI G'GCGC_C Cuts at: 2095 2095 Size: 7528 KpnI G_GTAC'C Cuts at: 2881 4853 2881 Size: 1972 5556 MfeI C'AATT_G Cuts at: 5127 5127 Size: 7528 MluI A'CGCG_T Cuts at: 7404 7404 Size: 7528 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 3712 4110 6642 6826 1305 Size: 880 1527 398 2532 184 2007 Fragments arranged by size: 2532 2007 1527 880 398 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2770 4809 10 Size: 54 1425 343 938 2039 2729 Fragments arranged by size: 2729 2039 1425 938 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2310 2591 2631 3131 5486 5845 6004 Size: 648 1144 281 40 500 2355 359 159 Cuts at: 6004 518 Size: 2042 Fragments arranged by size: 2355 2042 1144 648 500 359 281 159 40 MspA1I CmG'CkG Cuts at: 1606 2033 2045 2057 2199 2440 4317 4551 4944 Size: 427 12 12 142 241 1877 234 393 Cuts at: 4944 5621 6769 7014 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1877 1148 677 427 393 245 241 234 142 12 12 NaeI GCC'GGC Cuts at: 2132 2132 Size: 7528 NarI GG'CG_CC Cuts at: 2096 2096 Size: 7528 NcoI C'CATG_G Cuts at: 513 1065 2004 3632 4321 513 Size: 552 939 1628 689 3720 Fragments arranged by size: 3720 1628 939 689 552 NdeI CA'TA_TG Cuts at: 387 3209 387 Size: 2822 4706 NgoMIV G'CCGG_C Cuts at: 2130 2130 Size: 7528 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7528 NotI GC'GGCC_GC Cuts at: 4975 4975 Size: 7528 NsiI A_TGCA'T Cuts at: 2430 4487 2430 Size: 2057 5471 NspI r_CATG'y Cuts at: 1132 1229 3136 3359 4219 4886 6431 1132 Size: 97 1907 223 860 667 1545 2229 Fragments arranged by size: 2229 1907 1545 860 667 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7528 PciI A'CATG_T Cuts at: 6427 6427 Size: 7528 PflMI CCAn_nnn'nTGG Cuts at: 1108 3213 3920 1108 Size: 2105 707 4716 Fragments arranged by size: 4716 2105 707 PfoI T'CCnGG_A Cuts at: 4302 4421 4302 Size: 119 7409 PmeI GTTT'AAAC Cuts at: 4832 4832 Size: 7528 PmlI CAC'GTG Cuts at: 4248 4248 Size: 7528 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5597 5629 7110 7142 5597 Size: 32 1481 32 5983 Fragments arranged by size: 5983 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 3607 2725 Size: 882 6646 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7528 PsiI TTA'TAA Cuts at: 2420 5098 2420 Size: 2678 4850 PstI C_TGCA'G Cuts at: 830 4283 4880 830 Size: 3453 597 3478 Fragments arranged by size: 3478 3453 597 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7528 PvuII CAG'CTG Cuts at: 2045 2199 2440 4317 2045 Size: 154 241 1877 5256 Fragments arranged by size: 5256 1877 241 154 SacI G_AGCT'C Cuts at: 3594 3656 3594 Size: 62 7466 SacII CC_GC'GG Cuts at: 2034 2058 2034 Size: 24 7504 SalI G'TCGA_C Cuts at: 1936 4870 1936 Size: 2934 4594 SbfI CC_TGCA'GG Cuts at: 4880 4880 Size: 7528 ScaI AGT'ACT Cuts at: 5776 7488 5776 Size: 1712 5816 SfcI C'TryA_G Cuts at: 826 1045 3051 4279 4876 6011 6692 6883 826 Size: 219 2006 1228 597 1135 681 191 1471 Fragments arranged by size: 2006 1471 1228 1135 681 597 219 191 SfoI GGC'GCC Cuts at: 2097 2097 Size: 7528 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7528 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7528 SmaI CCC'GGG Cuts at: 1872 4855 1872 Size: 2983 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2388 3649 4408 4555 4790 5632 Size: 197 926 445 1261 759 147 235 842 Cuts at: 5632 6533 6795 820 Size: 901 262 1553 Fragments arranged by size: 1553 1261 926 901 842 759 445 262 235 197 147 SnaBI TAC'GTA Cuts at: 493 5319 493 Size: 4826 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7528 SphI G_CATG'C Cuts at: 1132 3136 4219 4886 1132 Size: 2004 1083 667 3774 Fragments arranged by size: 3774 2004 1083 667 SspI AAT'ATT Cuts at: 5 52 2810 5452 5 Size: 47 2758 2642 2081 Fragments arranged by size: 2758 2642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7528 StyI C'CwwG_G Cuts at: 513 1065 2004 3632 4321 4967 513 Size: 552 939 1628 689 646 3074 Fragments arranged by size: 3074 1628 939 689 646 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2743 5555 5572 5725 5910 6355 325 Size: 1255 1163 2812 17 153 185 445 1498 Fragments arranged by size: 2812 1498 1255 1163 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 3289 5774 Size: 274 80 33 51 1443 141 1171 2485 Cuts at: 5774 7486 96 Size: 1712 138 Fragments arranged by size: 2485 1712 1443 1171 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2982 3014 7488 7520 2982 Size: 32 4474 32 2990 Fragments arranged by size: 4474 2990 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7528 XbaI T'CTAG_A Cuts at: 4145 4864 4145 Size: 719 6809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3232 4619 3232 Size: 1387 6141 XhoI C'TCGA_G Cuts at: 1943 2388 1943 Size: 445 7083 XmaI C'CCGG_G Cuts at: 1870 4853 1870 Size: 2983 4545 XmnI GAAnn'nnTTC Cuts at: 2153 3568 4206 5657 2153 Size: 1415 638 1451 4024 Fragments arranged by size: 4024 1451 1415 638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5336 276 Size: 53 83 186 936 3802 2468 Fragments arranged by size: 3802 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI