(Circular) (Six-base) MAPSORT of: pFN21AB3384.seq Check: 3186 from: 1 to: 6802 pFN21AB3384 6802 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2753 2753 Size: 6802 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4612 278 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4145 1937 Size: 2208 4594 Acc65I G'GTAC_C Cuts at: 4123 4123 Size: 6802 AclI AA'CG_TT Cuts at: 1563 4929 5302 1563 Size: 3366 373 3063 Fragments arranged by size: 3366 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2763 3965 4865 6249 6517 1632 Size: 1131 1202 900 1384 268 1917 Fragments arranged by size: 1917 1384 1202 1131 900 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6605 AflIII A'CryG_T Cuts at: 1203 2518 3677 5701 6678 1203 Size: 1315 1159 2024 977 1327 Fragments arranged by size: 2024 1327 1315 1159 977 AgeI A'CCGG_T Cuts at: 5633 5633 Size: 6802 AhdI GACnn_n'nnGTC Cuts at: 5531 5531 Size: 6802 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6802 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6802 AlwNI CAG_nnn'CTG Cuts at: 2786 3050 3229 4545 6117 2786 Size: 264 179 1316 1572 3471 Fragments arranged by size: 3471 1572 1316 264 179 ApaLI G'TGCA_C Cuts at: 4859 6015 4859 Size: 1156 5646 ApoI r'AATT_y Cuts at: 1493 3369 3902 4096 4111 4337 4489 6598 1493 Size: 1876 533 194 15 226 152 2109 1697 Fragments arranged by size: 2109 1876 1697 533 226 194 152 15 AseI AT'TA_AT Cuts at: 160 1032 5356 160 Size: 872 4324 1606 Fragments arranged by size: 4324 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 AvaI C'yCGr_G Cuts at: 1870 1943 3374 3555 3897 4127 1870 Size: 73 1431 181 342 230 4545 Fragments arranged by size: 4545 1431 342 230 181 73 BamHI G'GATC_C Cuts at: 3416 3586 4132 4499 3416 Size: 170 546 367 5719 Fragments arranged by size: 5719 546 367 170 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4123 5578 618 Size: 325 217 627 2336 1455 1842 Fragments arranged by size: 2336 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 4121 1391 Size: 621 2109 4072 Fragments arranged by size: 4072 2109 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2524 3261 3672 3813 3831 928 Size: 971 625 737 411 141 18 3899 Fragments arranged by size: 3899 971 737 625 411 141 18 BbvCI CC'TCA_GC Cuts at: 2739 2959 3039 3072 4063 2739 Size: 220 80 33 991 5478 Fragments arranged by size: 5478 991 220 80 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2515 2549 4993 5027 1903 Size: 34 578 34 2444 34 3678 Fragments arranged by size: 3678 2444 578 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2508 4694 5904 2508 Size: 2186 1210 3406 Fragments arranged by size: 3406 2186 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6802 BfrBI ATG'CAT Cuts at: 2717 2717 Size: 6802 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 5413 136 Size: 107 122 71 631 605 361 3380 1525 Fragments arranged by size: 3380 1525 631 605 361 122 107 71 BglII A'GATC_T Cuts at: 6797 6797 Size: 6802 BlpI GC'TnA_GC Cuts at: 4219 4219 Size: 6802 Bme1580I G_kGCm'C Cuts at: 1790 2310 2383 4863 6019 1790 Size: 520 73 2480 1156 2573 Fragments arranged by size: 2573 2480 1156 520 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6802 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2442 2588 3540 Size: 416 53 83 399 24 1025 146 952 Cuts at: 3540 4533 5491 442 Size: 993 958 1753 Fragments arranged by size: 1753 1025 993 958 952 416 399 146 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6802 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2817 2817 Size: 6802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3239 3508 3760 3973 5462 1239 Size: 2000 269 252 213 1489 2579 Fragments arranged by size: 2579 2000 1489 269 252 213 Bpu10I CC'TnA_GC Cuts at: 2739 2959 3039 3072 4063 2739 Size: 220 80 33 991 5478 Fragments arranged by size: 5478 991 220 80 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 4927 5792 6090 2543 Size: 2384 865 298 3255 Fragments arranged by size: 3255 2384 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2253 5465 882 Size: 617 754 3212 2219 Fragments arranged by size: 3212 2219 754 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 4593 493 Size: 711 1961 1428 2702 Fragments arranged by size: 2702 1961 1428 711 BsaBI GATnn'nnATC Cuts at: 3135 6796 3135 Size: 3661 3141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4609 4991 5626 275 Size: 53 83 186 936 3076 382 635 1451 Fragments arranged by size: 3076 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5235 5633 5907 6054 6465 5235 Size: 398 274 147 411 5572 Fragments arranged by size: 5572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6577 Fragments arranged by size: 6577 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 2103 2189 2192 2216 2546 2760 2763 Size: 914 3 86 3 24 330 214 3 Cuts at: 2763 3044 3494 3933 1186 Size: 281 450 439 4055 Fragments arranged by size: 4055 914 450 439 330 281 214 86 24 3 3 3 BseYI C'CCAG_C Cuts at: 2331 2382 6005 6501 2331 Size: 51 3623 496 2632 Fragments arranged by size: 3623 2632 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2949 3990 1163 Size: 573 1213 1041 3975 Fragments arranged by size: 3975 1213 1041 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4252 5013 6041 1540 Size: 275 186 2251 761 1028 2301 Fragments arranged by size: 2301 2251 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2770 3746 4863 4948 6019 2012 Size: 758 976 1117 85 1071 2795 Fragments arranged by size: 2795 1117 1071 976 758 85 BsmI GAATG_Cn' Cuts at: 3601 4079 4313 4406 3601 Size: 478 234 93 5997 Fragments arranged by size: 5997 478 234 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3813 3813 Size: 6802 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2310 2383 2770 3746 4121 4863 Size: 399 222 298 73 387 976 375 742 Cuts at: 4863 4948 6019 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 976 742 399 387 375 298 222 85 73 BspEI T'CCGG_A Cuts at: 6465 6465 Size: 6802 BspHI T'CATG_A Cuts at: 4691 6421 4691 Size: 1730 5072 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2753 4157 844 Size: 1909 1404 3489 Fragments arranged by size: 3489 1909 1404 BsrBI CCG'CTC Cuts at: 1394 1823 3690 3713 4689 1394 Size: 429 1867 23 976 3507 Fragments arranged by size: 3507 1867 976 429 23 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 5297 5471 65 Size: 1183 1119 2930 174 1396 Fragments arranged by size: 2930 1396 1183 1119 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2274 5446 5633 1700 Size: 110 464 3172 187 2869 Fragments arranged by size: 3172 2869 464 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6802 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6802 BssSI C'ACGA_G Cuts at: 4862 5874 4862 Size: 1012 5790 BstAPI GCAn_nnn'nTGC Cuts at: 794 3050 794 Size: 2256 4546 BstBI TT'CG_AA Cuts at: 2535 5606 2535 Size: 3071 3731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6802 BstYI r'GATC_y Cuts at: 1972 2181 3416 3586 4132 4499 4884 4901 6353 Size: 209 1235 170 546 367 385 17 1452 Cuts at: 6353 6439 6451 6797 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1235 546 385 367 346 209 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3270 3270 Size: 6802 BtgI C'CryG_G Cuts at: 513 1065 3590 513 Size: 552 2525 3725 Fragments arranged by size: 3725 2525 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6063 Fragments arranged by size: 6063 683 56 BtsI GCAGTG_nn' Cuts at: 2796 4318 5112 5132 2796 Size: 1522 794 20 4466 Fragments arranged by size: 4466 1522 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6802 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6767 DraI TTT'AAA Cuts at: 4106 4106 Size: 6802 DraIII CAC_nnn'GTG Cuts at: 2751 3750 2751 Size: 999 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 5809 809 Size: 507 1155 3338 1802 Fragments arranged by size: 3338 1802 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2272 2545 Size: 54 1425 50 275 18 195 247 273 Cuts at: 2545 3190 4249 5138 6767 8 Size: 645 1059 889 1629 43 Fragments arranged by size: 1629 1425 1059 889 645 275 273 247 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4249 1537 Size: 275 2437 4090 Fragments arranged by size: 4090 2437 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2603 2861 3078 4732 1353 Size: 917 333 258 217 1654 3423 Fragments arranged by size: 3423 1654 917 333 258 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5389 5763 5909 387 Size: 1064 138 3800 374 146 1280 Fragments arranged by size: 3800 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 6802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2763 3239 3508 3760 3965 3973 4865 Size: 393 1131 476 269 252 205 8 892 Cuts at: 4865 5462 6249 6517 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1131 892 787 597 476 393 269 268 252 205 8 EcoNI CCTnn'n_nnAGG Cuts at: 2778 2778 Size: 6802 EcoO109I rG'GnC_Cy Cuts at: 2212 2626 2212 Size: 414 6388 EcoRI G'AATT_C Cuts at: 3902 4096 4111 4489 3902 Size: 194 15 378 6215 Fragments arranged by size: 6215 378 194 15 EcoRV GAT'ATC Cuts at: 2240 2240 Size: 6802 FspI TGC'GCA Cuts at: 5308 5308 Size: 6802 HaeII r_GCGC'y Cuts at: 1678 2896 5949 1678 Size: 1218 3053 2531 Fragments arranged by size: 3053 2531 1218 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5443 5475 5517 5549 1373 Size: 32 135 32 3871 32 42 32 2626 Fragments arranged by size: 3871 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4146 4392 669 Size: 988 281 2208 246 3079 Fragments arranged by size: 3079 2208 988 281 246 HindIII A'AGCT_T Cuts at: 748 2294 748 Size: 1546 5256 HpaI GTT'AAC Cuts at: 4392 4392 Size: 6802 KpnI G_GTAC'C Cuts at: 4127 4127 Size: 6802 MfeI C'AATT_G Cuts at: 4401 4401 Size: 6802 MluI A'CGCG_T Cuts at: 3677 6678 3677 Size: 3001 3801 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2601 5916 6100 1305 Size: 1296 3315 184 2007 Fragments arranged by size: 3315 2007 1296 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2547 3192 10 Size: 54 1425 343 195 520 645 3620 Fragments arranged by size: 3620 1425 645 520 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 3999 4760 5119 5278 518 Size: 648 2833 761 359 159 2042 Fragments arranged by size: 2833 2042 761 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2738 2963 3563 3592 4067 4218 4895 6043 Size: 1132 225 600 29 475 151 677 1148 Cuts at: 6043 6288 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1132 677 600 475 245 225 151 29 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6250 NdeI CA'TA_TG Cuts at: 387 2719 387 Size: 2332 4470 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6802 NotI GC'GGCC_GC Cuts at: 4249 4249 Size: 6802 NsiI A_TGCA'T Cuts at: 2719 2719 Size: 6802 NspI r_CATG'y Cuts at: 1132 1229 2522 4160 5705 1132 Size: 97 1293 1638 1545 2229 Fragments arranged by size: 2229 1638 1545 1293 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6802 PciI A'CATG_T Cuts at: 2518 5701 2518 Size: 3183 3619 PflMI CCAn_nnn'nTGG Cuts at: 1108 2751 3800 1108 Size: 1643 1049 4110 Fragments arranged by size: 4110 1643 1049 PfoI T'CCnGG_A Cuts at: 2012 2012 Size: 6802 PmeI GTTT'AAAC Cuts at: 4106 4106 Size: 6802 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3400 4871 4903 6384 6416 3368 Size: 32 1471 32 1481 32 3754 Fragments arranged by size: 3754 1481 1471 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2626 2212 Size: 414 6388 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6802 PsiI TTA'TAA Cuts at: 4372 4372 Size: 6802 PstI C_TGCA'G Cuts at: 830 2791 2901 4154 830 Size: 1961 110 1253 3478 Fragments arranged by size: 3478 1961 1253 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6802 PvuII CAG'CTG Cuts at: 2738 2963 3563 4067 2738 Size: 225 600 504 5473 Fragments arranged by size: 5473 600 504 225 SacI G_AGCT'C Cuts at: 2012 2012 Size: 6802 SacII CC_GC'GG Cuts at: 3593 3593 Size: 6802 SalI G'TCGA_C Cuts at: 1936 4144 1936 Size: 2208 4594 SbfI CC_TGCA'GG Cuts at: 2901 4154 2901 Size: 1253 5549 ScaI AGT'ACT Cuts at: 2541 3529 5050 6762 2541 Size: 988 1521 1712 2581 Fragments arranged by size: 2581 1712 1521 988 SfcI C'TryA_G Cuts at: 826 1045 2787 2817 2897 4150 5285 5966 6157 Size: 219 1742 30 80 1253 1135 681 191 Cuts at: 6157 826 Size: 1471 Fragments arranged by size: 1742 1471 1253 1135 681 219 191 80 30 SfiI GGCCn_nnn'nGGCC Cuts at: 2033 2033 Size: 6802 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6802 SmaI CCC'GGG Cuts at: 1872 3557 4129 1872 Size: 1685 572 4545 Fragments arranged by size: 4545 1685 572 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 4906 5807 6069 820 Size: 197 926 615 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 615 262 197 SnaBI TAC'GTA Cuts at: 493 4593 493 Size: 4100 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6802 SphI G_CATG'C Cuts at: 1132 4160 1132 Size: 3028 3774 SspI AAT'ATT Cuts at: 5 52 3893 4726 5 Size: 47 3841 833 2081 Fragments arranged by size: 3841 2081 833 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 5730 StyI C'CwwG_G Cuts at: 513 1065 2403 3866 4088 4241 513 Size: 552 1338 1463 222 153 3074 Fragments arranged by size: 3074 1463 1338 552 222 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3634 4829 4846 4999 5184 5629 325 Size: 1255 2054 1195 17 153 185 445 1498 Fragments arranged by size: 2054 1498 1255 1195 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2539 3527 3967 Size: 274 80 33 51 1443 562 988 440 Cuts at: 3967 5048 6760 96 Size: 1081 1712 138 Fragments arranged by size: 1712 1443 1081 988 562 440 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6762 6794 6762 Size: 32 6770 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6802 XbaI T'CTAG_A Cuts at: 4138 4138 Size: 6802 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3200 3200 Size: 6802 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6802 XmaI C'CCGG_G Cuts at: 1870 3555 4127 1870 Size: 1685 572 4545 Fragments arranged by size: 4545 1685 572 XmnI GAAnn'nnTTC Cuts at: 3514 3892 4044 4077 4931 3514 Size: 378 152 33 854 5385 Fragments arranged by size: 5385 854 378 152 33 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4610 276 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI