(Circular) (Six-base) MAPSORT of: pFN21AB9415.seq Check: 8912 from: 1 to: 6775 pFN21AB9415 6775 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2323 4118 1937 Size: 386 1795 4594 Fragments arranged by size: 4594 1795 386 Acc65I G'GTAC_C Cuts at: 3972 4096 3972 Size: 124 6651 AclI AA'CG_TT Cuts at: 1563 4902 5275 1563 Size: 3339 373 3063 Fragments arranged by size: 3339 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 2957 3189 4838 6222 6490 1632 Size: 417 908 232 1649 1384 268 1917 Fragments arranged by size: 1917 1649 1384 908 417 268 232 AflII C'TTAA_G Cuts at: 820 1017 3938 820 Size: 197 2921 3657 Fragments arranged by size: 3657 2921 197 AflIII A'CryG_T Cuts at: 1203 2111 2397 5674 6651 1203 Size: 908 286 3277 977 1327 Fragments arranged by size: 3277 1327 977 908 286 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6775 AlwNI CAG_nnn'CTG Cuts at: 3679 4518 6090 3679 Size: 839 1572 4364 Fragments arranged by size: 4364 1572 839 ApaLI G'TGCA_C Cuts at: 3361 4832 5988 3361 Size: 1471 1156 4148 Fragments arranged by size: 4148 1471 1156 ApoI r'AATT_y Cuts at: 1493 2013 2153 2335 2673 2861 2921 3225 3343 Size: 520 140 182 338 188 60 304 118 Cuts at: 3343 3609 4084 4310 4462 6571 1493 Size: 266 475 226 152 2109 1697 Fragments arranged by size: 2109 1697 520 475 338 304 266 226 188 182 152 140 118 60 AseI AT'TA_AT Cuts at: 160 1032 2595 5329 160 Size: 872 1563 2734 1606 Fragments arranged by size: 2734 1606 1563 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 2492 4100 1870 Size: 73 549 1608 4545 Fragments arranged by size: 4545 1608 549 73 BamHI G'GATC_C Cuts at: 4105 4472 4105 Size: 367 6408 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3972 4096 5551 618 Size: 325 217 627 2185 124 1455 1842 Fragments arranged by size: 2185 1842 1455 627 325 217 124 BanII G_rGCy'C Cuts at: 1391 4094 1391 Size: 2703 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4011 928 Size: 971 2112 3692 Fragments arranged by size: 3692 2112 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4966 5000 1903 Size: 34 3029 34 3678 Fragments arranged by size: 3678 3029 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3068 4667 5877 3068 Size: 1599 1210 3966 Fragments arranged by size: 3966 1599 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3215 3282 4041 Size: 107 122 71 631 605 1543 67 759 Cuts at: 4041 5386 136 Size: 1345 1525 Fragments arranged by size: 1543 1525 1345 759 631 605 122 107 71 67 BglII A'GATC_T Cuts at: 2950 6770 2950 Size: 3820 2955 BlpI GC'TnA_GC Cuts at: 3163 3622 4192 3163 Size: 459 570 5746 Fragments arranged by size: 5746 570 459 Bme1580I G_kGCm'C Cuts at: 1790 3365 4836 5992 1790 Size: 1575 1471 1156 2573 Fragments arranged by size: 2573 1575 1471 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4506 5464 442 Size: 416 53 83 399 24 3089 958 1753 Fragments arranged by size: 3089 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2813 1056 Size: 1757 5018 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2903 3760 3966 2903 Size: 857 206 5712 Fragments arranged by size: 5712 857 206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2913 5435 1239 Size: 1674 2522 2579 Fragments arranged by size: 2579 2522 1674 Bpu10I CC'TnA_GC Cuts at: 2466 2466 Size: 6775 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2292 4050 4900 5765 6063 2292 Size: 1758 850 865 298 3004 Fragments arranged by size: 3004 1758 865 850 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2158 5438 882 Size: 617 659 3280 2219 Fragments arranged by size: 3280 2219 659 617 BsaAI yAC'GTr Cuts at: 493 1204 4566 493 Size: 711 3362 2702 Fragments arranged by size: 3362 2702 711 BsaBI GATnn'nnATC Cuts at: 6769 6769 Size: 6775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4582 4964 5599 275 Size: 53 83 186 936 3049 382 635 1451 Fragments arranged by size: 3049 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5208 5606 5880 6027 6438 5208 Size: 398 274 147 411 5545 Fragments arranged by size: 5545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3833 3863 1178 Size: 30 165 30 2430 30 4090 Fragments arranged by size: 4090 2430 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3644 1186 Size: 2458 4317 BseYI C'CCAG_C Cuts at: 2962 3515 3546 5978 6474 2962 Size: 553 31 2432 496 3263 Fragments arranged by size: 3263 2432 553 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6202 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3365 4836 4921 5992 3365 Size: 1471 85 1071 4148 Fragments arranged by size: 4148 1471 1071 85 BsmI GAATG_Cn' Cuts at: 4286 4379 4286 Size: 93 6682 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3365 4094 4836 4921 5992 1391 Size: 399 1575 729 742 85 1071 2174 Fragments arranged by size: 2174 1575 1071 742 729 399 85 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4130 844 Size: 3286 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3621 3849 4662 1394 Size: 429 1798 228 813 3507 Fragments arranged by size: 3507 1798 813 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 5270 5444 65 Size: 1183 4022 174 1396 Fragments arranged by size: 4022 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2144 5419 5606 1700 Size: 110 334 3275 187 2869 Fragments arranged by size: 3275 2869 334 187 110 BsrGI T'GTAC_A Cuts at: 96 2182 96 Size: 2086 4689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 2888 4835 5847 2888 Size: 1947 1012 3816 Fragments arranged by size: 3816 1947 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3047 794 Size: 2253 4522 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6775 BstXI CCAn_nnnn'nTGG Cuts at: 3932 3932 Size: 6775 BstYI r'GATC_y Cuts at: 1972 2224 2582 2950 3299 3325 4105 4472 4857 Size: 252 358 368 349 26 780 367 385 Cuts at: 4857 4874 6326 6412 6424 6770 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 780 385 368 367 358 349 346 252 86 26 17 12 BstZ17I GTA'TAC Cuts at: 2324 2324 Size: 6775 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5284 Fragments arranged by size: 5284 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6036 Fragments arranged by size: 6036 683 56 BtsI GCAGTG_nn' Cuts at: 3157 4291 5085 5105 3157 Size: 1134 794 20 4827 Fragments arranged by size: 4827 1134 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6775 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6740 DraI TTT'AAA Cuts at: 2667 4079 2667 Size: 1412 5363 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5782 809 Size: 507 4466 1802 Fragments arranged by size: 4466 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4222 5111 6740 Size: 54 1425 50 275 18 2392 889 1629 Cuts at: 6740 8 Size: 43 Fragments arranged by size: 2392 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3292 4705 1353 Size: 1939 1413 3423 Fragments arranged by size: 3423 1939 1413 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2872 5362 5736 5882 387 Size: 1064 138 1283 2490 374 146 1280 Fragments arranged by size: 2490 1283 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2913 2957 3189 4838 5435 6222 Size: 393 417 864 44 232 1649 597 787 Cuts at: 6222 6490 1239 Size: 268 1524 Fragments arranged by size: 1649 1524 864 787 597 417 393 268 232 44 EcoNI CCTnn'n_nnAGG Cuts at: 2085 2085 Size: 6775 EcoO109I rG'GnC_Cy Cuts at: 2884 2884 Size: 6775 EcoRI G'AATT_C Cuts at: 2335 3343 4084 4462 2335 Size: 1008 741 378 4648 Fragments arranged by size: 4648 1008 741 378 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 5922 1678 Size: 4244 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2352 2384 3106 3138 5416 Size: 32 135 32 780 32 722 32 2278 Cuts at: 5416 5448 5490 5522 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2278 780 722 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2460 2613 4119 4365 669 Size: 988 281 522 153 1506 246 3079 Fragments arranged by size: 3079 1506 988 522 281 246 153 HindIII A'AGCT_T Cuts at: 748 748 Size: 6775 HpaI GTT'AAC Cuts at: 2613 4365 2613 Size: 1752 5023 KpnI G_GTAC'C Cuts at: 3976 4100 3976 Size: 124 6651 MfeI C'AATT_G Cuts at: 3051 3093 4374 3051 Size: 42 1281 5452 Fragments arranged by size: 5452 1281 42 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3674 5889 6073 1305 Size: 2369 2215 184 2007 Fragments arranged by size: 2369 2215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4953 Fragments arranged by size: 4953 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4733 5092 5251 518 Size: 648 3567 359 159 2042 Fragments arranged by size: 3567 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3206 3435 3676 4191 4868 6016 6261 1606 Size: 1600 229 241 515 677 1148 245 2120 Fragments arranged by size: 2120 1600 1148 677 515 245 241 229 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5284 Fragments arranged by size: 5284 939 552 NdeI CA'TA_TG Cuts at: 387 2059 387 Size: 1672 5103 NheI G'CTAG_C Cuts at: 1052 2809 1052 Size: 1757 5018 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 2115 2281 2401 2911 3203 4133 5678 Size: 97 886 166 120 510 292 930 1545 Cuts at: 5678 1132 Size: 2229 Fragments arranged by size: 2229 1545 930 886 510 292 166 120 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6775 PciI A'CATG_T Cuts at: 2111 2397 5674 2111 Size: 286 3277 3212 Fragments arranged by size: 3277 3212 286 PflMI CCAn_nnn'nTGG Cuts at: 1108 3012 1108 Size: 1904 4871 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2884 Size: 6775 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 4345 4345 Size: 6775 PstI C_TGCA'G Cuts at: 830 4127 830 Size: 3297 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 PvuII CAG'CTG Cuts at: 3206 3676 3206 Size: 470 6305 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SapI GCTCTTCn'nnn_ Cuts at: 3292 3292 Size: 6775 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 5023 6735 5023 Size: 1712 5063 SfcI C'TryA_G Cuts at: 826 1045 2760 2802 4123 5258 5939 6130 826 Size: 219 1715 42 1321 1135 681 191 1471 Fragments arranged by size: 1715 1471 1321 1135 681 219 191 42 SfiI GGCCn_nnn'nGGCC Cuts at: 4041 4041 Size: 6775 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 4102 1872 Size: 2230 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 3938 4065 4879 5780 6042 Size: 197 926 364 1631 127 814 901 262 Cuts at: 6042 820 Size: 1553 Fragments arranged by size: 1631 1553 926 901 814 364 262 197 127 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 2281 4133 1132 Size: 1149 1852 3774 Fragments arranged by size: 3774 1852 1149 SspI AAT'ATT Cuts at: 5 52 3664 4699 5 Size: 47 3612 1035 2081 Fragments arranged by size: 3612 2081 1035 47 StuI AGG'CCT Cuts at: 1517 3429 1517 Size: 1912 4863 StyI C'CwwG_G Cuts at: 513 1065 2004 4214 513 Size: 552 939 2210 3074 Fragments arranged by size: 3074 2210 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3307 4802 4819 4972 5157 5602 325 Size: 1255 1727 1495 17 153 185 445 1498 Fragments arranged by size: 1727 1498 1495 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2182 5021 6733 Size: 274 80 33 51 1443 205 2839 1712 Cuts at: 6733 96 Size: 138 Fragments arranged by size: 2839 1712 1443 274 205 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3688 3720 6735 6767 3688 Size: 32 3015 32 3696 Fragments arranged by size: 3696 3015 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6775 XbaI T'CTAG_A Cuts at: 3454 3878 4111 3454 Size: 424 233 6118 Fragments arranged by size: 6118 424 233 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3126 3126 Size: 6775 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6775 XmaI C'CCGG_G Cuts at: 1870 4100 1870 Size: 2230 4545 XmnI GAAnn'nnTTC Cuts at: 2013 4904 2013 Size: 2891 3884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI