(Circular) (Six-base) MAPSORT of: pFN21AB7614.seq Check: 4602 from: 1 to: 5785 pFN21AB7614 5785 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3128 1937 Size: 1191 4594 Acc65I G'GTAC_C Cuts at: 2966 3106 2966 Size: 140 5645 AclI AA'CG_TT Cuts at: 1563 3084 3912 4285 1563 Size: 1521 828 373 3063 Fragments arranged by size: 3063 1521 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2483 2639 3848 5232 5500 1632 Size: 851 156 1209 1384 268 1917 Fragments arranged by size: 1917 1384 1209 851 268 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 2592 4684 5661 1203 Size: 1389 2092 977 1327 Fragments arranged by size: 2092 1389 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2732 2764 2732 Size: 32 5753 AlwNI CAG_nnn'CTG Cuts at: 2168 3528 5100 2168 Size: 1360 1572 2853 Fragments arranged by size: 2853 1572 1360 ApaI G_GGCC'C Cuts at: 2238 2958 2238 Size: 720 5065 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2743 3094 3320 3472 5581 1493 Size: 1250 351 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 351 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2441 2810 3110 1870 Size: 73 498 369 300 4545 Fragments arranged by size: 4545 498 369 300 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2847 2966 3106 4561 Size: 325 217 627 576 484 119 140 1455 Cuts at: 4561 618 Size: 1842 Fragments arranged by size: 1842 1455 627 576 484 325 217 140 119 BanII G_rGCy'C Cuts at: 1391 2238 2346 2511 2811 2958 2987 3068 3104 Size: 847 108 165 300 147 29 81 36 Cuts at: 3104 1391 Size: 4072 Fragments arranged by size: 4072 847 300 165 147 108 81 36 29 BbeI G_GCGC'C Cuts at: 2851 2851 Size: 5785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2432 2466 3976 4010 1903 Size: 34 495 34 1510 34 3678 Fragments arranged by size: 3678 1510 495 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BfrBI ATG'CAT Cuts at: 3076 3076 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2074 2856 4396 Size: 107 122 71 631 605 402 782 1540 Cuts at: 4396 136 Size: 1525 Fragments arranged by size: 1540 1525 782 631 605 402 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 2111 2579 3202 2111 Size: 468 623 4694 Fragments arranged by size: 4694 623 468 Bme1580I G_kGCm'C Cuts at: 1790 2238 2262 2762 2958 3846 5002 1790 Size: 448 24 500 196 888 1156 2573 Fragments arranged by size: 2573 1156 888 500 448 196 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 2151 2396 2411 2759 4445 1239 Size: 792 120 245 15 348 1686 2579 Fragments arranged by size: 2579 1686 792 348 245 120 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 2916 4448 882 Size: 617 771 646 1532 2219 Fragments arranged by size: 2219 1532 771 646 617 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2848 3592 3974 4609 Size: 53 83 186 936 1315 744 382 635 Cuts at: 4609 275 Size: 1451 Fragments arranged by size: 1451 1315 936 744 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2295 2325 2732 2762 1178 Size: 30 165 30 892 30 407 30 4201 Fragments arranged by size: 4201 892 407 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2998 1186 Size: 1234 578 3973 Fragments arranged by size: 3973 1234 578 BseYI C'CCAG_C Cuts at: 2088 2796 2906 4988 5484 2088 Size: 708 110 2082 496 2389 Fragments arranged by size: 2389 2082 708 496 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2332 2705 2982 1163 Size: 573 596 373 277 3966 Fragments arranged by size: 3966 596 573 373 277 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 3931 5002 3846 Size: 85 1071 4629 Fragments arranged by size: 4629 1071 85 BsmI GAATG_Cn' Cuts at: 3076 3296 3389 3076 Size: 220 93 5472 Fragments arranged by size: 5472 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2262 2346 2511 2762 2811 2958 Size: 399 448 24 84 165 251 49 147 Cuts at: 2958 2987 3068 3104 3846 3931 5002 1391 Size: 29 81 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 251 165 147 85 84 81 49 36 29 24 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3140 844 Size: 2296 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2844 4429 4616 1700 Size: 110 1034 1585 187 2869 Fragments arranged by size: 2869 1585 1034 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 2681 3845 4857 2681 Size: 1164 1012 3609 Fragments arranged by size: 3609 1164 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2804 2804 Size: 5785 BstYI r'GATC_y Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780 Size: 1143 367 385 17 1452 86 12 346 Cuts at: 5780 1972 Size: 1977 Fragments arranged by size: 1977 1452 1143 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2289 3024 2289 Size: 735 5050 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2776 487 Size: 683 56 1550 3496 Fragments arranged by size: 3496 1550 683 56 BtsI GCAGTG_nn' Cuts at: 2762 2771 2994 3301 4095 4115 2762 Size: 9 223 307 794 20 4432 Fragments arranged by size: 4432 794 307 223 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750 Size: 54 1425 50 275 18 1402 889 1629 Cuts at: 5750 8 Size: 43 Fragments arranged by size: 1629 1425 1402 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2465 3715 1353 Size: 1112 1250 3423 Fragments arranged by size: 3423 1250 1112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2151 2396 2411 2483 2639 2759 Size: 393 399 120 245 15 72 156 120 Cuts at: 2759 3848 4445 5232 5500 1239 Size: 1089 597 787 268 1524 Fragments arranged by size: 1524 1089 787 597 399 393 268 245 156 120 120 72 15 EcoNI CCTnn'n_nnAGG Cuts at: 2130 2684 2919 2130 Size: 554 235 4996 Fragments arranged by size: 4996 554 235 EcoO109I rG'GnC_Cy Cuts at: 2123 2235 2331 2954 2955 2123 Size: 112 96 623 1 4953 Fragments arranged by size: 4953 623 112 96 1 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2851 4932 1678 Size: 1173 2081 2531 Fragments arranged by size: 2531 2081 1173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2350 4426 4458 4500 Size: 32 135 32 746 32 2076 32 42 Cuts at: 4500 4532 1373 Size: 32 2626 Fragments arranged by size: 2626 2076 746 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3129 3375 669 Size: 988 281 1191 246 3079 Fragments arranged by size: 3079 1191 988 281 246 HindIII A'AGCT_T Cuts at: 748 2113 748 Size: 1365 4420 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KasI G'GCGC_C Cuts at: 2847 2847 Size: 5785 KpnI G_GTAC'C Cuts at: 2970 3110 2970 Size: 140 5645 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 4899 5083 1305 Size: 919 2675 184 2007 Fragments arranged by size: 2675 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3963 Fragments arranged by size: 3963 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3743 4102 4261 518 Size: 648 2577 359 159 2042 Fragments arranged by size: 2577 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3201 3878 5026 5271 1606 Size: 1595 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 245 NarI GG'CG_CC Cuts at: 2848 2848 Size: 5785 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4294 Fragments arranged by size: 4294 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NsiI A_TGCA'T Cuts at: 3078 3078 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 2596 3143 4688 1132 Size: 97 1367 547 1545 2229 Fragments arranged by size: 2229 1545 1367 547 97 PasI CC'CwG_GG Cuts at: 1348 2482 2515 2951 1348 Size: 1134 33 436 4182 Fragments arranged by size: 4182 1134 436 33 PciI A'CATG_T Cuts at: 2592 4684 2592 Size: 2092 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 2054 2978 1108 Size: 946 924 3915 Fragments arranged by size: 3915 946 924 PfoI T'CCnGG_A Cuts at: 2128 2128 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2732 2764 3854 3886 5367 5399 2732 Size: 32 1090 32 1481 32 3118 Fragments arranged by size: 3118 1481 1090 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2932 1613 Size: 1319 4466 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspOMI G'GGCC_C Cuts at: 2234 2954 2234 Size: 720 5065 PspXI vC'TCGA_Gb Cuts at: 2441 2441 Size: 5785 PstI C_TGCA'G Cuts at: 830 2433 2577 2619 2757 2778 3137 830 Size: 1603 144 42 138 21 359 3478 Fragments arranged by size: 3478 1603 359 144 138 42 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2429 2573 2615 2753 2774 3133 4268 Size: 219 1384 144 42 138 21 359 1135 Cuts at: 4268 4949 5140 826 Size: 681 191 1471 Fragments arranged by size: 1471 1384 1135 681 359 219 191 144 138 42 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2074 2074 Size: 5785 SfoI GGC'GCC Cuts at: 2849 2849 Size: 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2441 2810 3889 4790 5052 820 Size: 197 926 498 369 1079 901 262 1553 Fragments arranged by size: 1553 1079 926 901 498 369 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2263 3224 513 Size: 552 939 115 144 961 3074 Fragments arranged by size: 3074 961 939 552 144 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2614 3001 3812 3829 3982 4167 4612 Size: 1255 1034 387 811 17 153 185 445 Cuts at: 4612 325 Size: 1498 Fragments arranged by size: 1498 1255 1034 811 445 387 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3003 4031 5743 Size: 274 80 33 51 1443 1026 1028 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1712 1443 1028 1026 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2295 2327 5745 5777 2295 Size: 32 3418 32 2303 Fragments arranged by size: 3418 2303 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2126 2126 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 2441 2810 1943 Size: 498 369 4918 Fragments arranged by size: 4918 498 369 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 2537 2652 2747 3036 3914 2537 Size: 115 95 289 878 4408 Fragments arranged by size: 4408 878 289 115 95 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI