(Circular) (Six-base) MAPSORT of: pFN21AB8692.seq Check: 5536 from: 1 to: 6142 pFN21AB8692 6142 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 3382 4269 4642 1563 Size: 1819 887 373 3063 Fragments arranged by size: 3063 1819 887 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3152 3216 4205 5589 5857 1632 Size: 1520 64 989 1384 268 1917 Fragments arranged by size: 1917 1520 1384 989 268 64 AfeI AGC'GCT Cuts at: 2529 2529 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 2987 820 Size: 197 1970 3975 Fragments arranged by size: 3975 1970 197 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 2891 4871 2891 Size: 1980 4162 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 2956 3885 5457 2956 Size: 929 1572 3641 Fragments arranged by size: 3641 1572 929 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 2920 3451 3677 3829 5938 1493 Size: 1427 531 226 152 2109 1697 Fragments arranged by size: 2109 1697 1427 531 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2172 2439 3467 1870 Size: 73 229 267 1028 4545 Fragments arranged by size: 4545 1028 267 229 73 AvrII C'CTAG_G Cuts at: 3272 3272 Size: 6142 BamHI G'GATC_C Cuts at: 2374 3472 3839 2374 Size: 1098 367 4677 Fragments arranged by size: 4677 1098 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2514 3463 4918 618 Size: 325 217 627 727 949 1455 1842 Fragments arranged by size: 1842 1455 949 727 627 325 217 BanII G_rGCy'C Cuts at: 1391 2345 3461 1391 Size: 954 1116 4072 Fragments arranged by size: 4072 1116 954 BbeI G_GCGC'C Cuts at: 2518 2518 Size: 6142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 3159 928 Size: 971 804 456 3911 Fragments arranged by size: 3911 971 804 456 BbvCI CC'TCA_GC Cuts at: 2361 2361 Size: 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 2925 1548 Size: 1377 4765 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3091 6137 3091 Size: 3046 3096 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 4203 5359 1790 Size: 2413 1156 2573 Fragments arranged by size: 2573 2413 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2373 3873 4831 Size: 416 53 83 399 24 956 1500 958 Cuts at: 4831 442 Size: 1753 Fragments arranged by size: 1753 1500 958 956 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2255 1056 Size: 1199 4943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 4802 1239 Size: 1517 2046 2579 Fragments arranged by size: 2579 2046 1517 Bpu10I CC'TnA_GC Cuts at: 2182 2187 2361 2182 Size: 5 174 5963 Fragments arranged by size: 5963 174 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2818 4267 5132 5430 2818 Size: 1449 865 298 3530 Fragments arranged by size: 3530 1449 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 3294 6136 3294 Size: 2842 3300 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2515 3949 4331 4966 Size: 53 83 186 936 982 1434 382 635 Cuts at: 4966 275 Size: 1451 Fragments arranged by size: 1451 1434 982 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2729 2759 1178 Size: 30 165 30 1326 30 4561 Fragments arranged by size: 4561 1326 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 2246 2249 2252 2255 2258 2318 2321 Size: 1048 12 3 3 3 3 60 3 Cuts at: 2321 2324 2327 2330 2483 2486 2498 2501 2504 Size: 3 3 3 153 3 12 3 3 Cuts at: 2504 2507 1186 Size: 3 4821 Fragments arranged by size: 4821 1048 153 60 12 12 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2875 5345 5841 2875 Size: 2470 496 3176 Fragments arranged by size: 3176 2470 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3432 1163 Size: 573 1696 3873 Fragments arranged by size: 3873 1696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2345 4203 4288 5359 2345 Size: 1858 85 1071 3128 Fragments arranged by size: 3128 1858 1071 85 BsmI GAATG_Cn' Cuts at: 2299 2326 3405 3653 3746 2299 Size: 27 1079 248 93 4695 Fragments arranged by size: 4695 1079 248 93 27 BsmBI CGTCTCn'nnnn_ Cuts at: 2208 2208 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2345 3461 4203 4288 5359 1391 Size: 399 555 1116 742 85 1071 2174 Fragments arranged by size: 2174 1116 1071 742 555 399 85 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 2158 4031 5761 2158 Size: 1873 1730 2539 Fragments arranged by size: 2539 1873 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 3497 844 Size: 1210 1443 3489 Fragments arranged by size: 3489 1443 1210 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2445 4786 4973 1700 Size: 110 635 2341 187 2869 Fragments arranged by size: 2869 2341 635 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2015 2374 3091 3472 3839 4224 4241 5693 Size: 43 359 717 381 367 385 17 1452 Cuts at: 5693 5779 5791 6137 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 717 385 381 367 359 346 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 2586 2586 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4651 Fragments arranged by size: 4651 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 3658 4452 4472 3658 Size: 794 20 5328 Fragments arranged by size: 5328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2705 5149 809 Size: 507 1389 2444 1802 Fragments arranged by size: 2444 1802 1389 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3589 4478 6107 Size: 54 1425 50 275 18 1759 889 1629 Cuts at: 6107 8 Size: 43 Fragments arranged by size: 1759 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3322 3340 3369 4072 1353 Size: 1969 18 29 703 3423 Fragments arranged by size: 3423 1969 703 29 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4729 5103 5249 387 Size: 1064 138 3140 374 146 1280 Fragments arranged by size: 3140 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2343 2343 Size: 6142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2756 3152 3216 4205 4802 5589 5857 Size: 393 1124 396 64 989 597 787 268 Cuts at: 5857 1239 Size: 1524 Fragments arranged by size: 1524 1124 989 787 597 396 393 268 64 EcoO109I rG'GnC_Cy Cuts at: 2066 2066 Size: 6142 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2518 2531 5289 1678 Size: 840 13 2758 2531 Fragments arranged by size: 2758 2531 840 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2877 2909 4783 Size: 32 135 32 581 32 692 32 1874 Cuts at: 4783 4815 4857 4889 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1874 692 581 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2456 2715 2982 3486 3732 669 Size: 988 281 518 259 267 504 246 3079 Fragments arranged by size: 3079 988 518 504 281 267 259 246 HindIII A'AGCT_T Cuts at: 748 3434 748 Size: 2686 3456 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KasI G'GCGC_C Cuts at: 2514 2514 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5256 5440 1305 Size: 3951 184 2007 Fragments arranged by size: 3951 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4320 Fragments arranged by size: 4320 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4100 4459 4618 518 Size: 648 2934 359 159 2042 Fragments arranged by size: 2934 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2512 3558 4235 5383 5628 1606 Size: 442 464 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 464 442 245 NaeI GCC'GGC Cuts at: 2447 2447 Size: 6142 NarI GG'CG_CC Cuts at: 2515 2515 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4651 Fragments arranged by size: 4651 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 2445 2445 Size: 6142 NheI G'CTAG_C Cuts at: 1052 2251 1052 Size: 1199 4943 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3500 5045 1132 Size: 97 2271 1545 2229 Fragments arranged by size: 2271 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6142 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2882 1108 Size: 1774 4368 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3339 3371 4211 4243 5724 5756 3339 Size: 32 840 32 1481 32 3725 Fragments arranged by size: 3725 1481 840 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 2066 Size: 6142 PshAI GACnn'nnGTC Cuts at: 1613 2609 1613 Size: 996 5146 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 3494 830 Size: 2664 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2048 2048 Size: 6142 SacI G_AGCT'C Cuts at: 2345 2345 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SapI GCTCTTCn'nnn_ Cuts at: 3322 3369 3322 Size: 47 6095 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 3137 4390 6102 3137 Size: 1253 1712 3177 Fragments arranged by size: 3177 1712 1253 SfcI C'TryA_G Cuts at: 826 1045 3490 4625 5306 5497 826 Size: 219 2445 1135 681 191 1471 Fragments arranged by size: 2445 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2516 2516 Size: 6142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 2174 3469 1872 Size: 302 1295 4545 Fragments arranged by size: 4545 1295 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 2797 2987 4246 5147 5409 820 Size: 197 926 854 190 1259 901 262 1553 Fragments arranged by size: 1553 1259 926 901 854 262 197 190 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2366 2567 1517 Size: 849 201 5092 Fragments arranged by size: 5092 849 201 StyI C'CwwG_G Cuts at: 513 1065 2004 2058 3272 3581 513 Size: 552 939 54 1214 309 3074 Fragments arranged by size: 3074 1214 939 552 309 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4186 4339 4524 4969 325 Size: 1255 2589 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2203 2808 3135 Size: 274 80 33 51 1443 226 605 327 Cuts at: 3135 4388 6100 96 Size: 1253 1712 138 Fragments arranged by size: 1712 1443 1253 605 327 274 226 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 2172 3467 1870 Size: 302 1295 4545 Fragments arranged by size: 4545 1295 302 XmnI GAAnn'nnTTC Cuts at: 3015 4271 3015 Size: 1256 4886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI