Restriction map for
pFN21AB8604
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AocI 987 CCTGAGG CC^T[ACGT]AGG
ApaI 209 GGGCCC GGGCC^C
AspI 709 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 662 TGGCCA TGG^CCA
BanI 1147 GGTGCC G^G[CT][GA]CC
BlpI 196 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 196 GCTGAGC GC^T[ACGT]AGC
BsaI 1081 GAGACC GGTCTC(1/5)
BsgI 409 GTGCAG GTGCAG(16/14)
BsmBI 499 GAGACG CGTCTC(1/5)
BsrBI 58 CCGCTC CCGCTC(-3/-3)
BsrFI 1118 GCCGGC [GA]^CCGG[CT]
BsrI 78 ACTGG ACTGG(1/-1)
BstAPI 4 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 468 GGATCT [GA]^GATC[CT]
Bsu36I 987 CCTGAGG CC^T[ACGT]AGG
CelII 196 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1118 GCCGGC [GA]^CCGG[CT]
DrdI 445 GACTGTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 987 CCTGAGG CC^T[ACGT]AGG
HaeII 763 AGCGCC [GA]GCGC^[CT]
HgaI 315 GACGC GACGC(5/10)
HinfI 520 GACTC G^A[ACGT]TC
MflI 468 GGATCT [GA]^GATC[CT]
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
NaeI 1118 GCCGGC GCC^GGC
NgoMIV 1118 GCCGGC G^CCGGC
NspI 1059 GCATGC [GA]CATG^[CT]
PaeR7I 61 CTCGAG C^TCGAG
PflFI 709 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 520 GACTC GAGTC(4/5)
PpuMI 50 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 315 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 847 CAGCTG CAG^CTG
SacI 1036 GAGCTC GAGCT^C
ScaI 527 AGTACT AGT^ACT
SmlI 61 CTCGAG C^T[CT][GA]AG
SphI 1059 GCATGC GCATG^C
Tth111I 709 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 761 CCAGCGCCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 61 CTCGAG C^TCGAG
XhoII 468 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 384 GACGTC GACGT^C
451 GACGTC
AcyI 384 GACGTC G[GA]^CG[CT]C
451 GACGTC
AlwI 1026 GATCC GGATC(4/5)
468 GGATC
AlwNI 460 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
799 CAGCTCCTG
BbsI 312 GAAGAC GAAGAC(2/6)
603 GAAGAC
BglI 539 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
742 GCCATCAAGGC
BpuAI 312 GAAGAC GAAGAC(2/6)
603 GAAGAC
BsaHI 384 GACGTC G[GA]^CG[CT]C
451 GACGTC
BsaWI 668 ACCGGA [AT]^CCGG[AT]
1094 ACCGGA
BsmAI 499 GAGAC GTCTC(1/5)
1081 GAGAC
BstEII 366 GGTCACC G^GT[ACGT]ACC
594 GGTGACC
BstPI 366 GGTCACC G^GT[ACGT]ACC
594 GGTGACC
BstXI 268 CCAGAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1162 CCATGGAAGTGG
DpnII 469 GATC ^GATC
1026 GATC
EcoO65I 366 GGTCACC G^GT[ACGT]ACC
594 GGTGACC
Hin1I 384 GACGTC G[GA]^CG[CT]C
451 GACGTC
MboI 469 GATC ^GATC
1026 GATC
NdeII 469 GATC ^GATC
1026 GATC
PflMI 459 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1157 CCATCCCATGG
Sau3AI 469 GATC ^GATC
1026 GATC
Van91I 459 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1157 CCATCCCATGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I