(Circular) (Six-base) MAPSORT of: pFN21AB8604.seq Check: 4834 from: 1 to: 5944 pFN21AB8604 5944 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2393 2460 3754 278 Size: 53 83 186 936 857 67 1294 2468 Fragments arranged by size: 2468 1294 936 857 186 83 67 53 AccI GT'mk_AC Cuts at: 1937 3287 1937 Size: 1350 4594 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 3243 4071 4444 1563 Size: 1680 828 373 3063 Fragments arranged by size: 3063 1680 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2876 3026 4007 5391 5659 1632 Size: 995 249 150 981 1384 268 1917 Fragments arranged by size: 1917 1384 995 981 268 249 150 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 2411 1166 Size: 1245 4699 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2429 2915 1184 Size: 1245 486 4213 Fragments arranged by size: 4213 1245 486 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2420 2452 2771 2803 2420 Size: 32 319 32 5561 Fragments arranged by size: 5561 319 32 32 AlwNI CAG_nnn'CTG Cuts at: 2470 2809 3687 5259 2470 Size: 339 878 1572 3155 Fragments arranged by size: 3155 1572 878 339 ApaI G_GGCC'C Cuts at: 2218 2218 Size: 5944 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2223 2249 3269 1870 Size: 73 123 157 26 1020 4545 Fragments arranged by size: 4545 1020 157 123 73 26 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3152 3265 4720 618 Size: 325 217 627 1365 113 1455 1842 Fragments arranged by size: 1842 1455 1365 627 325 217 113 BanII G_rGCy'C Cuts at: 1391 2034 2218 2250 2256 2564 3045 3263 1391 Size: 643 184 32 6 308 481 218 4072 Fragments arranged by size: 4072 643 481 308 218 184 32 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 2615 928 Size: 971 425 291 4257 Fragments arranged by size: 4257 971 425 291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 1548 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2550 2753 4555 Size: 107 122 71 631 605 878 203 1802 Cuts at: 4555 136 Size: 1525 Fragments arranged by size: 1802 1525 878 631 605 203 122 107 71 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 2202 3361 2202 Size: 1159 4785 Bme1580I G_kGCm'C Cuts at: 1790 2218 3143 4005 5161 1790 Size: 428 925 862 1156 2573 Fragments arranged by size: 2573 1156 925 862 428 BmgBI CAC'GTC Cuts at: 1318 2497 1318 Size: 1179 4765 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3675 4633 442 Size: 416 53 83 399 24 2258 958 1753 Fragments arranged by size: 2258 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2417 2615 2654 2749 2924 4604 1239 Size: 950 228 198 39 95 175 1680 2579 Fragments arranged by size: 2579 1680 950 228 198 175 95 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4069 4934 5232 4069 Size: 865 298 4781 Fragments arranged by size: 4781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3080 4607 882 Size: 617 1581 1527 2219 Fragments arranged by size: 2219 1581 1527 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2390 2457 3751 4133 Size: 53 83 186 936 857 67 1294 382 Cuts at: 4133 4768 275 Size: 635 1451 Fragments arranged by size: 1451 1294 936 857 635 382 186 83 67 53 BsaWI w'CCGG_w Cuts at: 2673 3099 4377 4775 5049 5196 5607 2673 Size: 426 1278 398 274 147 411 3010 Fragments arranged by size: 3010 1278 426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 1178 Size: 30 165 30 1017 30 4672 Fragments arranged by size: 4672 1017 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2135 2138 2147 2210 2435 2807 2810 Size: 940 9 3 9 63 225 372 3 Cuts at: 2810 1186 Size: 4320 Fragments arranged by size: 4320 940 372 225 63 9 9 3 3 BseYI C'CCAG_C Cuts at: 2260 2958 3109 3119 5147 5643 2260 Size: 698 151 10 2028 496 2561 Fragments arranged by size: 2561 2028 698 496 151 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 1163 Size: 573 699 4672 Fragments arranged by size: 4672 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2910 3045 3083 4005 4090 5161 2910 Size: 135 38 922 85 1071 3693 Fragments arranged by size: 3693 1071 922 135 85 38 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 BsmBI CGTCTCn'nnnn_ Cuts at: 2498 2498 Size: 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2218 2250 2256 2564 2910 3045 Size: 399 244 184 32 6 308 346 135 Cuts at: 3045 3083 3143 3263 4005 4090 5161 1391 Size: 38 60 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 308 244 184 135 120 85 60 38 32 6 BspEI T'CCGG_A Cuts at: 5607 5607 Size: 5944 BspHI T'CATG_A Cuts at: 3833 5563 3833 Size: 1730 4214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3299 844 Size: 2455 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2065 3831 1394 Size: 429 242 1766 3507 Fragments arranged by size: 3507 1766 429 242 BsrDI GCAATG_nn' Cuts at: 65 1248 4439 4613 65 Size: 1183 3191 174 1396 Fragments arranged by size: 3191 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3123 4588 4775 1700 Size: 110 1313 1465 187 2869 Fragments arranged by size: 2869 1465 1313 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 4004 5016 4004 Size: 1012 4932 BstAPI GCAn_nnn'nTGC Cuts at: 794 2015 794 Size: 1221 4723 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 2371 2599 1184 Size: 1187 228 4529 Fragments arranged by size: 4529 1187 228 BstXI CCAn_nnnn'nTGG Cuts at: 2280 3174 2280 Size: 894 5050 BstYI r'GATC_y Cuts at: 1972 2473 3274 3641 4026 4043 5495 5581 5593 Size: 501 801 367 385 17 1452 86 12 Cuts at: 5593 5939 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 801 501 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2993 2993 Size: 5944 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 2511 3167 513 Size: 552 939 237 270 656 3290 Fragments arranged by size: 3290 939 656 552 270 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3214 487 Size: 683 56 1988 3217 Fragments arranged by size: 3217 1988 683 56 BtsI GCAGTG_nn' Cuts at: 2042 2517 3460 4254 4274 2042 Size: 475 943 794 20 3712 Fragments arranged by size: 3712 943 794 475 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5909 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2456 4951 809 Size: 507 1140 2495 1802 Fragments arranged by size: 2495 1802 1140 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2508 2667 2724 Size: 54 1425 50 275 18 678 159 57 Cuts at: 2724 3391 4280 5909 8 Size: 667 889 1629 43 Fragments arranged by size: 1629 1425 889 678 667 275 159 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2124 2133 2271 2939 3874 1353 Size: 759 12 9 138 668 935 3423 Fragments arranged by size: 3423 935 759 668 138 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2207 2726 4531 4905 5051 387 Size: 1064 138 618 519 1805 374 146 1280 Fragments arranged by size: 1805 1280 1064 618 519 374 146 138 EcoICRI GAG'CTC Cuts at: 3043 3043 Size: 5944 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2417 2615 2627 2654 2749 2876 Size: 393 557 228 198 12 27 95 127 Cuts at: 2876 2924 3026 4007 4604 5391 5659 1239 Size: 48 102 981 597 787 268 1524 Fragments arranged by size: 1524 981 787 597 557 393 268 228 198 127 102 95 48 27 12 EcoO109I rG'GnC_Cy Cuts at: 2056 2214 2215 2467 2056 Size: 158 1 252 5533 Fragments arranged by size: 5533 252 158 1 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 5944 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2772 5091 1678 Size: 1094 2319 2531 Fragments arranged by size: 2531 2319 1094 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4585 4617 4659 4691 1373 Size: 32 135 32 3013 32 42 32 2626 Fragments arranged by size: 3013 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5944 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3045 5058 5242 1305 Size: 1740 2013 184 2007 Fragments arranged by size: 2013 2007 1740 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 3285 Fragments arranged by size: 3285 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 2495 3172 3902 4261 4420 518 Size: 648 1245 84 677 730 359 159 2042 Fragments arranged by size: 2042 1245 730 677 648 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2167 2707 2854 3076 3360 4037 5185 5430 Size: 561 540 147 222 284 677 1148 245 Cuts at: 5430 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 561 540 284 245 222 147 NaeI GCC'GGC Cuts at: 3125 3125 Size: 5944 NcoI C'CATG_G Cuts at: 513 1065 2004 2241 2511 3167 513 Size: 552 939 237 270 656 3290 Fragments arranged by size: 3290 939 656 552 270 237 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NgoMIV G'CCGG_C Cuts at: 3123 3123 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 3068 3302 4847 1132 Size: 97 1839 234 1545 2229 Fragments arranged by size: 2229 1839 1545 234 97 PasI CC'CwG_GG Cuts at: 1348 2330 1348 Size: 982 4962 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 2470 3168 1108 Size: 1362 698 3884 Fragments arranged by size: 3884 1362 698 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2420 2452 4013 4045 5526 5558 2420 Size: 32 1561 32 1481 32 2806 Fragments arranged by size: 2806 1561 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2056 Size: 5944 PshAI GACnn'nnGTC Cuts at: 1613 2324 1613 Size: 711 5233 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PspOMI G'GGCC_C Cuts at: 2214 2214 Size: 5944 PspXI vC'TCGA_Gb Cuts at: 2066 2066 Size: 5944 PstI C_TGCA'G Cuts at: 830 2388 2814 2894 3296 830 Size: 1558 426 80 402 3478 Fragments arranged by size: 3478 1558 426 402 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 PvuII CAG'CTG Cuts at: 2854 2854 Size: 5944 SacI G_AGCT'C Cuts at: 3045 3045 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SanDI GG'GwC_CC Cuts at: 2056 2056 Size: 5944 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 2534 4192 5904 2534 Size: 1658 1712 2574 Fragments arranged by size: 2574 1712 1658 SfcI C'TryA_G Cuts at: 826 1045 2384 2810 2890 3292 4427 5108 5299 Size: 219 1339 426 80 402 1135 681 191 Cuts at: 5299 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 681 426 402 219 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 3271 1872 Size: 1399 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2066 4048 4949 5211 820 Size: 197 926 123 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 123 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3068 3302 1132 Size: 1936 234 3774 Fragments arranged by size: 3774 1936 234 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5944 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2511 2548 3167 3201 3383 Size: 552 939 237 270 37 619 34 182 Cuts at: 3383 513 Size: 3074 Fragments arranged by size: 3074 939 619 552 270 237 182 37 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 3971 3988 4141 4326 4771 325 Size: 1255 855 1536 17 153 185 445 1498 Fragments arranged by size: 1536 1498 1255 855 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 2970 4190 Size: 274 80 33 51 1443 555 438 1220 Cuts at: 4190 5902 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1220 555 438 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 2717 1316 Size: 1401 4543 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2773 2773 Size: 5944 XhoI C'TCGA_G Cuts at: 1943 2066 1943 Size: 123 5821 XmaI C'CCGG_G Cuts at: 1870 3269 1870 Size: 1399 4545 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2391 2458 3752 276 Size: 53 83 186 936 857 67 1294 2468 Fragments arranged by size: 2468 1294 936 857 186 83 67 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI