Restriction map for
pFN21AB8604
(Linear) (Six-base) MAPSORT of: KIBB8604.seq Check: 4254 from: 1 to: 1239
KIBB8604.orf
With 182 enzymes: SgfI *
February 20, 2009 16:36 ..
AatII G_ACGT'C
Cuts at: 0 388 455 1239
Size: 388 67 784
Fragments arranged by size:
784 388 67
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 871 1021 1239
Size: 622 249 150 218
Fragments arranged by size:
622 249 218 150
AleI CACnn'nnGTG
Cuts at: 0 406 1239
Size: 406 833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 910 1239
Size: 424 486 329
Fragments arranged by size:
486 424 329
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 766 798 1239
Size: 415 32 319 32 441
Fragments arranged by size:
441 415 319 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 465 804 1239
Size: 465 339 435
Fragments arranged by size:
465 435 339
ApaI G_GGCC'C
Cuts at: 0 213 1239
Size: 213 1026
AvaI C'yCGr_G
Cuts at: 0 61 218 244 1239
Size: 61 157 26 995
Fragments arranged by size:
995 157 61 26
BanI G'GyrC_C
Cuts at: 0 1147 1239
Size: 1147 92
BanII G_rGCy'C
Cuts at: 0 29 213 245 251 559 1040 1239
Size: 29 184 32 6 308 481 199
Fragments arranged by size:
481 308 199 184 32 29 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 610 1239
Size: 319 291 629
Fragments arranged by size:
629 319 291
BglI GCCn_nnn'nGGC
Cuts at: 0 545 748 1239
Size: 545 203 491
Fragments arranged by size:
545 491 203
BlpI GC'TnA_GC
Cuts at: 0 197 1239
Size: 197 1042
Bme1580I G_kGCm'C
Cuts at: 0 213 1138 1239
Size: 213 925 101
Fragments arranged by size:
925 213 101
BmgBI CAC'GTC
Cuts at: 0 492 1239
Size: 492 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 412 610 649 744 919 1239
Size: 184 228 198 39 95 175 320
Fragments arranged by size:
320 228 198 184 175 95 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 1075 1239
Size: 1075 164
BsaHI Gr'CG_yC
Cuts at: 0 385 452 1239
Size: 385 67 787
Fragments arranged by size:
787 385 67
BsaWI w'CCGG_w
Cuts at: 0 668 1094 1239
Size: 668 426 145
Fragments arranged by size:
668 426 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 1239
Size: 415 30 794
Fragments arranged by size:
794 415 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 130 133 142 205 430 802 805
Size: 121 9 3 9 63 225 372 3
Cuts at: 805 1239
Size: 434
Fragments arranged by size:
434 372 225 121 63 9 9 3
3
BseYI C'CCAG_C
Cuts at: 0 255 953 1104 1114 1239
Size: 255 698 151 10 125
Fragments arranged by size:
698 255 151 125 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1239
Size: 430 809
BsiHKAI G_wGCw'C
Cuts at: 0 905 1040 1078 1239
Size: 905 135 38 161
Fragments arranged by size:
905 161 135 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 493 1239
Size: 493 746
Bsp1286I G_dGCh'C
Cuts at: 0 29 213 245 251 559 905 1040 1078
Size: 29 184 32 6 308 346 135 38
Cuts at: 1078 1138 1239
Size: 60 101
Fragments arranged by size:
346 308 184 135 101 60 38 32
29 6
BsrBI CCG'CTC
Cuts at: 0 60 1239
Size: 60 1179
BsrFI r'CCGG_y
Cuts at: 0 1118 1239
Size: 1118 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 1239
Size: 10 1229
BstEII G'GTnAC_C
Cuts at: 0 366 594 1239
Size: 366 228 645
Fragments arranged by size:
645 366 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 275 1169 1239
Size: 275 894 70
Fragments arranged by size:
894 275 70
BstYI r'GATC_y
Cuts at: 0 468 1239
Size: 468 771
Bsu36I CC'TnA_GG
Cuts at: 0 988 1239
Size: 988 251
BtgI C'CryG_G
Cuts at: 0 236 506 1162 1239
Size: 236 270 656 77
Fragments arranged by size:
656 270 236 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1209 1239
Size: 1209 30
BtsI GCAGTG_nn'
Cuts at: 0 37 512 1239
Size: 37 475 727
Fragments arranged by size:
727 475 37
DrdI GACnn_nn'nnGTC
Cuts at: 0 451 1239
Size: 451 788
EaeI y'GGCC_r
Cuts at: 0 503 662 719 1239
Size: 503 159 57 520
Fragments arranged by size:
520 503 159 57
EarI CTCTTCn'nnn_
Cuts at: 0 107 119 128 266 934 1239
Size: 107 12 9 138 668 305
Fragments arranged by size:
668 305 138 107 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 721 1239
Size: 202 519 518
Fragments arranged by size:
519 518 202
EcoICRI GAG'CTC
Cuts at: 0 1038 1239
Size: 1038 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 412 610 622 649 744 871 919
Size: 184 228 198 12 27 95 127 48
Cuts at: 919 1021 1239
Size: 102 218
Fragments arranged by size:
228 218 198 184 127 102 95 48
27 12
EcoO109I rG'GnC_Cy
Cuts at: 0 51 209 210 462 1239
Size: 51 158 1 252 777
Fragments arranged by size:
777 252 158 51 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 945 1239
Size: 945 294
HaeII r_GCGC'y
Cuts at: 0 767 1239
Size: 767 472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1239
Size: 1040 199
MscI TGG'CCA
Cuts at: 0 664 1239
Size: 664 575
MslI CAynn'nnrTG
Cuts at: 0 406 490 1167 1239
Size: 406 84 677 72
Fragments arranged by size:
677 406 84 72
MspA1I CmG'CkG
Cuts at: 0 162 702 849 1071 1239
Size: 162 540 147 222 168
Fragments arranged by size:
540 222 168 162 147
NaeI GCC'GGC
Cuts at: 0 1120 1239
Size: 1120 119
NcoI C'CATG_G
Cuts at: 0 236 506 1162 1239
Size: 236 270 656 77
Fragments arranged by size:
656 270 236 77
NgoMIV G'CCGG_C
Cuts at: 0 1118 1239
Size: 1118 121
NspI r_CATG'y
Cuts at: 0 1063 1239
Size: 1063 176
PasI CC'CwG_GG
Cuts at: 0 325 1239
Size: 325 914
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 1163 1239
Size: 465 698 76
Fragments arranged by size:
698 465 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 1239
Size: 415 32 792
Fragments arranged by size:
792 415 32
PpuMI rG'GwC_Cy
Cuts at: 0 51 1239
Size: 51 1188
PshAI GACnn'nnGTC
Cuts at: 0 319 1239
Size: 319 920
PspOMI G'GGCC_C
Cuts at: 0 209 1239
Size: 209 1030
PspXI vC'TCGA_Gb
Cuts at: 0 61 1239
Size: 61 1178
PstI C_TGCA'G
Cuts at: 0 383 809 889 1239
Size: 383 426 80 350
Fragments arranged by size:
426 383 350 80
PvuII CAG'CTG
Cuts at: 0 849 1239
Size: 849 390
SacI G_AGCT'C
Cuts at: 0 1040 1239
Size: 1040 199
SanDI GG'GwC_CC
Cuts at: 0 51 1239
Size: 51 1188
ScaI AGT'ACT
Cuts at: 0 529 1239
Size: 529 710
SfcI C'TryA_G
Cuts at: 0 379 805 885 1239
Size: 379 426 80 354
Fragments arranged by size:
426 379 354 80
SmlI C'TyrA_G
Cuts at: 0 61 1239
Size: 61 1178
SphI G_CATG'C
Cuts at: 0 1063 1239
Size: 1063 176
StyI C'CwwG_G
Cuts at: 0 236 506 543 1162 1196 1239
Size: 236 270 37 619 34 43
Fragments arranged by size:
619 270 236 43 37 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 1239
Size: 430 809
TatI w'GTAC_w
Cuts at: 0 527 965 1239
Size: 527 438 274
Fragments arranged by size:
527 438 274
Tth111I GACn'n_nGTC
Cuts at: 0 712 1239
Size: 712 527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 1239
Size: 768 471
XhoI C'TCGA_G
Cuts at: 0 61 1239
Size: 61 1178
ZraI GAC'GTC
Cuts at: 0 386 453 1239
Size: 386 67 786
Fragments arranged by size:
786 386 67
Enzymes that do cut:
AatII AcuI AleI AlfI AloI AlwNI ApaI AvaI
BanI BanII BbsI BglI BlpI Bme1580I BmgBI BpmI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BsrBI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI
ScaI SfcI SmlI SphI StyI TaqII TatI Tth111I
XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I ClaI CspCI DraI DraIII EagI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PsiI PsrI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TstI XbaI XmaI
XmnI