Restriction map for
pFN21AB9746
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AvaI AvaII
AviII AvrII BalI BanI BanII
BbeI BbvCI BbvI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FbaI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
ItaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI TseI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 101 GTAGAC GT^[AC][GT]AC
Alw44I 1156 GTGCAC G^TGCAC
ApaLI 1156 GTGCAC G^TGCAC
AspHI 1156 GTGCAC G[AT]GC[AT]^C
BamHI 1016 GGATCC G^GATCC
BbrPI 113 CACGTG CAC^GTG
BcnI 500 CCCGG CC^[GC]GG
BmyI 1156 GTGCAC G[AGT]GC[ACT]^C
BpmI 1109 CTGGAG CTGGAG(16/14)
BseRI 544 GAGGAG GAGGAG(10/8)
BsiHKAI 1156 GTGCAC G[AT]GC[AT]^C
BsiYI 1020 CCATACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1020 CCATACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1156 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 1254 TGTACA T^GTACA
BsrDI 378 GCAATG GCAATG(2/0)
BsrGI 1254 TGTACA T^GTACA
BstAPI 878 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 874 GGATG GGATG(2/0)
BstNI 579 CCTGG CC^[AT]GG
BstYI 1016 GGATCC [GA]^GATC[CT]
DpnII 1017 GATC ^GATC
DraI 1543 TTTAAA TTT^AAA
EcoRII 579 CCTGG ^CC[AT]GG
FokI 874 GGATG GGATG(9/13)
HapII 501 CCGG C^CGG
HpaII 501 CCGG C^CGG
MaeIII 1382 GTAAC ^GT[ACGT]AC
MboI 1017 GATC ^GATC
MflI 1016 GGATCC [GA]^GATC[CT]
MspA1I 1516 CAGCGG C[AC]G^C[GT]G
MspI 501 CCGG C^CGG
MvaI 579 CCTGG CC^[AT]GG
NciI 500 CCCGG CC^[GC]GG
NdeII 1017 GATC ^GATC
NspI 264 GCATGT [GA]CATG^[CT]
PleI 151 GACTC GAGTC(4/5)
PmaCI 113 CACGTG CAC^GTG
PmlI 113 CACGTG CAC^GTG
Sau3AI 1017 GATC ^GATC
SexAI 578 ACCTGGT A^CC[AT]GGT
SspBI 1254 TGTACA T^GTACA
TfiI 1268 GAATC G^A[AT]TC
XhoII 1016 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AhdI 138 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1230 GACTTTTAGTC
AlwI 1017 GATCC GGATC(4/5)
1016 GGATC
AspEI 138 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1230 GACTTTTAGTC
BbsI 929 GAAGAC GAAGAC(2/6)
1563 GAAGAC
BfaI 481 CTAG C^TAG
653 CTAG
BlpI 320 GCTCAGC GC^T[ACGT]AGC
667 GCTAAGC
Bpu1102I 320 GCTCAGC GC^T[ACGT]AGC
667 GCTAAGC
BpuAI 929 GAAGAC GAAGAC(2/6)
1563 GAAGAC
BsaAI 113 CACGTG [CT]AC^GT[GA]
1159 CACGTA
BsmI 622 GAATGC GAATGC(1/-1)
883 GAATGC
BssKI 500 CCCGG ^CC[ACGT]GG
579 CCTGG
CelII 320 GCTCAGC GC^T[ACGT]AGC
667 GCTAAGC
Eam1105I 138 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1230 GACTTTTAGTC
HindIII 473 AAGCTT A^AGCTT
493 AAGCTT
HinfI 151 GACTC G^A[ACGT]TC
1268 GAATC
MaeI 481 CTAG C^TAG
653 CTAG
MaeII 114 ACGT A^CGT
1160 ACGT
MwoI 495 GCTTACCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
878 GCAAGGAATGC
NdeI 449 CATATG CA^TATG
1609 CATATG
NlaIV 3 GGAACC GG[ACGT]^[ACGT]CC
1016 GGATCC
ScaI 511 AGTACT AGT^ACT
985 AGTACT
ScrFI 500 CCCGG CC^[ACGT]GG
579 CCTGG
TaqI 753 TCGA T^CGA
1239 TCGA
TthHB8I 753 TCGA T^CGA
1239 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BsrI DdeI EcoRI FauI
HaeIII HphI MboII MnlI MseI
MslI NlaIII RsaI SfaNI StuI
Tru9I Tsp509I TspRI XmnI