(Circular) (Six-base) MAPSORT of: pFN21AB9738.seq Check: 7705 from: 1 to: 6214 pFN21AB9738 6214 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2199 2199 Size: 6214 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3208 4277 5661 5929 1632 Size: 1576 1069 1384 268 1917 Fragments arranged by size: 1917 1576 1384 1069 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 2035 2309 5113 6090 1203 Size: 832 274 2804 977 1327 Fragments arranged by size: 2804 1327 977 832 274 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 2843 3100 1166 Size: 1677 257 4280 Fragments arranged by size: 4280 1677 257 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2861 2921 1184 Size: 1677 60 4477 Fragments arranged by size: 4477 1677 60 AlwNI CAG_nnn'CTG Cuts at: 2079 2101 3316 3444 3957 5529 2079 Size: 22 1215 128 513 1572 2764 Fragments arranged by size: 2764 1572 1215 513 128 22 ApaI G_GGCC'C Cuts at: 2466 2748 3109 2466 Size: 282 361 5571 Fragments arranged by size: 5571 361 282 ApaLI G'TGCA_C Cuts at: 3383 4271 5427 3383 Size: 888 1156 4170 Fragments arranged by size: 4170 1156 888 ApoI r'AATT_y Cuts at: 1493 3251 3523 3749 3901 6010 1493 Size: 1758 272 226 152 2109 1697 Fragments arranged by size: 2109 1758 1697 272 226 152 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2246 2338 2741 2926 3539 1870 Size: 73 303 92 403 185 613 4545 Fragments arranged by size: 4545 613 403 303 185 92 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3535 4990 618 Size: 325 217 627 1748 1455 1842 Fragments arranged by size: 1842 1748 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2466 2694 2748 2856 3015 3109 3533 1391 Size: 1075 228 54 108 159 94 424 4072 Fragments arranged by size: 4072 1075 424 228 159 108 94 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3223 3408 928 Size: 971 1324 185 3734 Fragments arranged by size: 3734 1324 971 185 BbvCI CC'TCA_GC Cuts at: 3437 3437 Size: 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2470 4825 136 Size: 107 122 71 631 605 798 2355 1525 Fragments arranged by size: 2355 1525 798 631 605 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 2375 3631 2375 Size: 1256 4958 Bme1580I G_kGCm'C Cuts at: 1790 2133 2466 2748 3109 3262 3387 3398 4275 Size: 343 333 282 361 153 125 11 877 Cuts at: 4275 5431 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 877 361 343 333 282 153 125 11 BmgBI CAC'GTC Cuts at: 1318 2875 1318 Size: 1557 4657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2676 3145 3945 Size: 416 53 83 399 24 1259 469 800 Cuts at: 3945 4903 442 Size: 958 1753 Fragments arranged by size: 1753 1259 958 800 469 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2072 2072 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2540 2658 2700 3101 3521 4874 1239 Size: 1301 118 42 401 420 1353 2579 Fragments arranged by size: 2579 1353 1301 420 401 118 42 Bpu10I CC'TnA_GC Cuts at: 2159 3437 2159 Size: 1278 4936 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4339 5204 5502 4339 Size: 865 298 5051 Fragments arranged by size: 5051 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4877 882 Size: 617 3378 2219 Fragments arranged by size: 3378 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3061 4005 493 Size: 711 1857 944 2702 Fragments arranged by size: 2702 1857 944 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2617 2647 1178 Size: 30 165 30 1214 30 4745 Fragments arranged by size: 4745 1214 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6214 BseYI C'CCAG_C Cuts at: 2527 2699 2735 2748 2855 2959 2982 2999 3178 Size: 172 36 13 107 104 23 17 179 Cuts at: 3178 3323 5417 5913 2527 Size: 145 2094 496 2828 Fragments arranged by size: 2828 2094 496 179 172 145 107 104 36 23 17 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2821 2917 1163 Size: 573 1085 96 4460 Fragments arranged by size: 4460 1085 573 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2352 3664 4425 5453 1540 Size: 275 186 351 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 351 275 186 BsiHKAI G_wGCw'C Cuts at: 3015 3387 4275 4360 5431 3015 Size: 372 888 85 1071 3798 Fragments arranged by size: 3798 1071 888 372 85 BsmI GAATG_Cn' Cuts at: 2993 3725 3818 2993 Size: 732 93 5389 Fragments arranged by size: 5389 732 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2720 2720 Size: 6214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2466 2694 2748 2856 3015 3109 Size: 399 343 333 228 54 108 159 94 Cuts at: 3109 3262 3387 3398 3533 4275 4360 5431 1391 Size: 153 125 11 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 333 228 159 153 135 125 108 94 85 54 11 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 2977 3569 844 Size: 1355 778 592 3489 Fragments arranged by size: 3489 1355 778 592 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4709 4883 65 Size: 1183 3461 174 1396 Fragments arranged by size: 3461 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2270 4858 5045 1700 Size: 110 460 2588 187 2869 Fragments arranged by size: 2869 2588 460 187 110 BsrGI T'GTAC_A Cuts at: 96 2886 96 Size: 2790 3424 BssHII G'CGCG_C Cuts at: 1925 2147 2174 2266 1925 Size: 222 27 92 5873 Fragments arranged by size: 5873 222 92 27 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 2079 794 Size: 1285 4929 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3102 3298 2011 Size: 1091 196 4927 Fragments arranged by size: 4927 1091 196 BstYI r'GATC_y Cuts at: 1972 2614 3544 3911 4296 4313 5765 5851 5863 Size: 642 930 367 385 17 1452 86 12 Cuts at: 5863 6209 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 930 642 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 2754 2778 2841 3271 513 Size: 552 939 199 551 24 63 430 3456 Fragments arranged by size: 3456 939 552 551 430 199 63 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2102 2297 2568 3730 4524 4544 2102 Size: 195 271 1162 794 20 3772 Fragments arranged by size: 3772 1162 794 271 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 2365 2459 2365 Size: 94 6120 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2963 3227 5221 809 Size: 507 1647 264 1994 1802 Fragments arranged by size: 1994 1802 1647 507 264 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2213 2349 3661 Size: 54 1425 50 275 18 383 136 1312 Cuts at: 3661 4550 6179 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1312 889 383 275 136 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2349 3661 1537 Size: 275 537 1312 4090 Fragments arranged by size: 4090 1312 537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3335 4144 1353 Size: 1982 809 3423 Fragments arranged by size: 3423 1982 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3151 3162 4801 5175 5321 387 Size: 1064 138 1562 11 1639 374 146 1280 Fragments arranged by size: 1639 1562 1280 1064 374 146 138 11 EcoICRI GAG'CTC Cuts at: 3013 3013 Size: 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2540 2658 2700 3101 3208 3521 4277 Size: 393 908 118 42 401 107 313 756 Cuts at: 4277 4874 5661 5929 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 908 787 756 597 401 393 313 268 118 107 42 EcoNI CCTnn'n_nnAGG Cuts at: 2162 2555 2162 Size: 393 5821 EcoO109I rG'GnC_Cy Cuts at: 2155 2344 2431 2463 2513 2545 2670 2745 3313 Size: 189 87 32 50 32 125 75 568 Cuts at: 3313 2155 Size: 5056 Fragments arranged by size: 5056 568 189 125 87 75 50 32 32 EcoRI G'AATT_C Cuts at: 3251 3523 3901 3251 Size: 272 378 5564 Fragments arranged by size: 5564 378 272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2672 2672 Size: 6214 FspI TGC'GCA Cuts at: 2296 2303 4720 2296 Size: 7 2417 3790 Fragments arranged by size: 3790 2417 7 HaeII r_GCGC'y Cuts at: 1678 2835 2870 3271 3459 5361 1678 Size: 1157 35 401 188 1902 2531 Fragments arranged by size: 2531 1902 1157 401 188 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3398 3430 4855 4887 4929 Size: 32 135 32 1826 32 1425 32 42 Cuts at: 4929 4961 1373 Size: 32 2626 Fragments arranged by size: 2626 1826 1425 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3558 3804 669 Size: 988 281 1620 246 3079 Fragments arranged by size: 3079 1620 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3813 3813 Size: 6214 MluI A'CGCG_T Cuts at: 2035 2309 6090 2035 Size: 274 3781 2159 Fragments arranged by size: 3781 2159 274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5328 5512 1305 Size: 4023 184 2007 Fragments arranged by size: 4023 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4392 Fragments arranged by size: 4392 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2490 2843 3100 4172 4531 4690 518 Size: 648 1324 353 257 1072 359 159 2042 Fragments arranged by size: 2042 1324 1072 648 359 353 257 159 MspA1I CmG'CkG Cuts at: 1606 2076 2205 2292 2441 2531 2756 2780 2843 Size: 470 129 87 149 90 225 24 63 Cuts at: 2843 2963 3031 3100 3323 3630 4307 5455 5700 Size: 120 68 69 223 307 677 1148 245 Cuts at: 5700 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 470 307 245 225 223 149 129 120 90 87 69 68 63 24 NaeI GCC'GGC Cuts at: 2272 2272 Size: 6214 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NgoMIV G'CCGG_C Cuts at: 2270 2270 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 2495 3572 5117 1132 Size: 97 1266 1077 1545 2229 Fragments arranged by size: 2229 1545 1266 1077 97 PasI CC'CwG_GG Cuts at: 1348 2468 2687 2861 3068 1348 Size: 1120 219 174 207 4494 Fragments arranged by size: 4494 1120 219 207 174 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2459 3244 1108 Size: 1351 785 4078 Fragments arranged by size: 4078 1351 785 PfoI T'CCnGG_A Cuts at: 2635 3015 2635 Size: 380 5834 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PmlI CAC'GTG Cuts at: 3061 3061 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2344 2545 3313 2155 Size: 189 201 768 5056 Fragments arranged by size: 5056 768 201 189 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PspOMI G'GGCC_C Cuts at: 2462 2744 3105 2462 Size: 282 361 5571 Fragments arranged by size: 5571 361 282 PstI C_TGCA'G Cuts at: 830 2097 2910 3566 830 Size: 1267 813 656 3478 Fragments arranged by size: 3478 1267 813 656 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2441 2531 2963 3031 3100 3323 2441 Size: 90 432 68 69 223 5332 Fragments arranged by size: 5332 432 223 90 69 68 SacI G_AGCT'C Cuts at: 3015 3015 Size: 6214 SacII CC_GC'GG Cuts at: 2206 2757 2781 2844 2206 Size: 551 24 63 5576 Fragments arranged by size: 5576 551 63 24 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SanDI GG'GwC_CC Cuts at: 2344 2344 Size: 6214 SapI GCTCTTCn'nnn_ Cuts at: 3335 3335 Size: 6214 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SfcI C'TryA_G Cuts at: 826 1045 2093 2906 3562 4697 5378 5569 826 Size: 219 1048 813 656 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 813 681 656 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2470 2470 Size: 6214 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 2743 3541 1872 Size: 871 798 4545 Fragments arranged by size: 4545 871 798 SmlI C'TyrA_G Cuts at: 820 1017 1943 4318 5219 5481 820 Size: 197 926 2375 901 262 1553 Fragments arranged by size: 2375 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 2189 1517 Size: 672 5542 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2059 2628 3333 3653 513 Size: 552 939 6 49 569 705 320 3074 Fragments arranged by size: 3074 939 705 569 552 320 49 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 4258 4411 4596 5041 325 Size: 1255 2661 17 153 185 445 1498 Fragments arranged by size: 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2886 3132 4460 Size: 274 80 33 51 1443 909 246 1328 Cuts at: 4460 6172 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1328 909 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 2599 1316 Size: 1283 4931 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 2741 3539 1870 Size: 871 798 4545 Fragments arranged by size: 4545 871 798 XmnI GAAnn'nnTTC Cuts at: 2141 2655 4343 2141 Size: 514 1688 4012 Fragments arranged by size: 4012 1688 514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SexAI SfoI SrfI SwaI XcmI