(Circular) (Six-base) MAPSORT of: pFN21AB9703.seq Check: 470 from: 1 to: 5689 pFN21AB9703 5689 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AfeI AGC'GCT Cuts at: 2244 2244 Size: 5689 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2494 3432 5004 2494 Size: 938 1572 3179 Fragments arranged by size: 3179 1572 938 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2260 2405 2743 3014 1870 Size: 73 90 227 145 338 271 4545 Fragments arranged by size: 4545 338 271 227 145 90 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 2454 2724 2783 3010 Size: 325 217 627 533 134 270 59 227 Cuts at: 3010 4465 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 533 325 270 227 217 134 59 BanII G_rGCy'C Cuts at: 1391 2658 3008 1391 Size: 1267 350 4072 Fragments arranged by size: 4072 1267 350 BbeI G_GCGC'C Cuts at: 2324 2458 2728 2324 Size: 134 270 5285 Fragments arranged by size: 5285 270 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 928 Size: 971 189 4529 Fragments arranged by size: 4529 971 189 BbvCI CC'TCA_GC Cuts at: 2648 2648 Size: 5689 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2390 2781 4300 Size: 107 122 71 631 605 718 391 1519 Cuts at: 4300 136 Size: 1525 Fragments arranged by size: 1525 1519 718 631 605 391 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2786 3750 4906 1790 Size: 996 964 1156 2573 Fragments arranged by size: 2573 1156 996 964 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2243 1056 Size: 1187 4502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2132 4349 1239 Size: 803 90 2217 2579 Fragments arranged by size: 2579 2217 803 90 Bpu10I CC'TnA_GC Cuts at: 2162 2426 2648 2162 Size: 264 222 5203 Fragments arranged by size: 5203 264 222 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2855 3814 4679 4977 2855 Size: 959 865 298 3567 Fragments arranged by size: 3567 959 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2321 2455 2547 Size: 53 83 186 936 513 275 134 92 Cuts at: 2547 2725 3496 3878 4513 275 Size: 178 771 382 635 1451 Fragments arranged by size: 1451 936 771 635 513 382 275 186 178 134 92 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5689 BseYI C'CCAG_C Cuts at: 2801 4892 5388 2801 Size: 2091 496 3102 Fragments arranged by size: 3102 2091 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2681 1163 Size: 573 945 4171 Fragments arranged by size: 4171 945 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2232 3139 3900 4928 1540 Size: 275 186 231 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 231 186 BsiHKAI G_wGCw'C Cuts at: 2028 2658 3750 3835 4906 2028 Size: 630 1092 85 1071 2811 Fragments arranged by size: 2811 1092 1071 630 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 BsmBI CGTCTCn'nnnn_ Cuts at: 2306 2306 Size: 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2658 2786 3008 3750 3835 4906 Size: 399 238 630 128 222 742 85 1071 Cuts at: 4906 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 630 399 238 222 128 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2679 3044 844 Size: 1835 365 3489 Fragments arranged by size: 3489 1835 365 BsrBI CCG'CTC Cuts at: 1394 1823 2430 2435 2521 3576 1394 Size: 429 607 5 86 1055 3507 Fragments arranged by size: 3507 1055 607 429 86 5 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2457 4333 4520 1700 Size: 110 647 1876 187 2869 Fragments arranged by size: 2869 1876 647 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2177 2362 2657 3749 4761 2177 Size: 185 295 1092 1012 3105 Fragments arranged by size: 3105 1092 1012 295 185 BstAPI GCAn_nnn'nTGC Cuts at: 794 2494 794 Size: 1700 3989 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2136 487 Size: 683 56 910 4040 Fragments arranged by size: 4040 910 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2505 2625 Size: 54 1425 50 275 18 399 276 120 Cuts at: 2625 3136 4025 5654 8 Size: 511 889 1629 43 Fragments arranged by size: 1629 1425 889 511 399 276 275 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2229 3136 1537 Size: 275 417 907 4090 Fragments arranged by size: 4090 907 417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 2495 4276 4650 4796 387 Size: 1064 138 561 345 1781 374 146 1280 Fragments arranged by size: 1781 1280 1064 561 374 345 146 138 EcoICRI GAG'CTC Cuts at: 2656 2656 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2132 3752 4349 5136 5404 1239 Size: 393 410 90 1620 597 787 268 1524 Fragments arranged by size: 1620 1524 787 597 410 393 268 90 EcoNI CCTnn'n_nnAGG Cuts at: 2603 2603 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2038 2104 2266 2917 2038 Size: 66 162 651 4810 Fragments arranged by size: 4810 651 162 66 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2246 2324 2458 2599 2728 4836 1678 Size: 568 78 134 141 129 2108 2531 Fragments arranged by size: 2531 2108 568 141 134 129 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2829 2861 2969 3001 4330 Size: 32 135 32 1257 32 108 32 1329 Cuts at: 4330 4362 4404 4436 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 1257 135 108 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2320 2454 2724 2320 Size: 134 270 5285 Fragments arranged by size: 5285 270 134 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 10 Size: 54 1425 343 795 3072 Fragments arranged by size: 3072 1425 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3647 4006 4165 518 Size: 648 2481 359 159 2042 Fragments arranged by size: 2481 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2494 3105 3782 4930 5175 1606 Size: 888 611 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 677 611 245 NaeI GCC'GGC Cuts at: 2459 2459 Size: 5689 NarI GG'CG_CC Cuts at: 2321 2455 2725 2321 Size: 134 270 5285 Fragments arranged by size: 5285 270 134 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2457 2457 Size: 5689 NheI G'CTAG_C Cuts at: 1052 2239 1052 Size: 1187 4502 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2533 3047 4592 1132 Size: 97 1304 514 1545 2229 Fragments arranged by size: 2229 1545 1304 514 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2904 1108 Size: 1796 3893 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2173 3758 3790 5271 5303 2141 Size: 32 1585 32 1481 32 2527 Fragments arranged by size: 2527 1585 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2917 2104 Size: 813 4876 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 2217 2490 3041 830 Size: 1387 273 551 3478 Fragments arranged by size: 3478 1387 551 273 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2494 2494 Size: 5689 SacI G_AGCT'C Cuts at: 2658 2658 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SanDI GG'GwC_CC Cuts at: 2104 2104 Size: 5689 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2183 2397 3937 5649 2183 Size: 214 1540 1712 2223 Fragments arranged by size: 2223 1712 1540 214 SfcI C'TryA_G Cuts at: 826 1045 2213 2486 3037 4172 4853 5044 826 Size: 219 1168 273 551 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 681 551 273 219 191 SfoI GGC'GCC Cuts at: 2322 2456 2726 2322 Size: 134 270 5285 Fragments arranged by size: 5285 270 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 2457 1700 Size: 757 4932 SmaI CCC'GGG Cuts at: 1872 2035 2407 2745 3016 1872 Size: 163 372 338 271 4545 Fragments arranged by size: 4545 372 338 271 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 2870 3793 4694 4956 820 Size: 197 926 927 923 901 262 1553 Fragments arranged by size: 1553 927 926 923 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 2533 3047 1132 Size: 1401 514 3774 Fragments arranged by size: 3774 1401 514 SrfI GCCC'GGGC Cuts at: 2745 2745 Size: 5689 SspI AAT'ATT Cuts at: 5 52 2884 3613 5 Size: 47 2832 729 2081 Fragments arranged by size: 2832 2081 729 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2004 2604 3128 513 Size: 552 939 600 524 3074 Fragments arranged by size: 3074 939 600 552 524 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2395 3935 Size: 274 80 33 51 1443 204 214 1540 Cuts at: 3935 5647 96 Size: 1712 138 Fragments arranged by size: 1712 1540 1443 274 214 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2033 2405 2743 3014 1870 Size: 163 372 338 271 4545 Fragments arranged by size: 4545 372 338 271 163 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SwaI XcmI