Restriction map for
pFN21AB9703
(Linear) (Six-base) MAPSORT of: KIBB9703.seq Check: 8595 from: 1 to: 984
KIBB9703.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AfeI AGC'GCT
Cuts at: 0 239 984
Size: 239 745
AlwNI CAG_nnn'CTG
Cuts at: 0 489 984
Size: 489 495
AvaI C'yCGr_G
Cuts at: 0 28 255 400 738 984
Size: 28 227 145 338 246
Fragments arranged by size:
338 246 227 145 28
BanI G'GyrC_C
Cuts at: 0 315 449 719 778 984
Size: 315 134 270 59 206
Fragments arranged by size:
315 270 206 134 59
BanII G_rGCy'C
Cuts at: 0 653 984
Size: 653 331
BbeI G_GCGC'C
Cuts at: 0 319 453 723 984
Size: 319 134 270 261
Fragments arranged by size:
319 270 261 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 984
Size: 83 901
BbvCI CC'TCA_GC
Cuts at: 0 643 984
Size: 643 341
BglI GCCn_nnn'nGGC
Cuts at: 0 385 776 984
Size: 385 391 208
Fragments arranged by size:
391 385 208
Bme1580I G_kGCm'C
Cuts at: 0 781 984
Size: 781 203
BmtI G_CTAG'C
Cuts at: 0 238 984
Size: 238 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 127 984
Size: 37 90 857
Fragments arranged by size:
857 90 37
Bpu10I CC'TnA_GC
Cuts at: 0 157 421 643 984
Size: 157 264 222 341
Fragments arranged by size:
341 264 222 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 984
Size: 850 134
BsaHI Gr'CG_yC
Cuts at: 0 41 316 450 542 720 984
Size: 41 275 134 92 178 264
Fragments arranged by size:
275 264 178 134 92 41
BseYI C'CCAG_C
Cuts at: 0 796 984
Size: 796 188
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 984
Size: 676 308
BsiEI CG_ry'CG
Cuts at: 0 227 984
Size: 227 757
BsiHKAI G_wGCw'C
Cuts at: 0 23 653 984
Size: 23 630 331
Fragments arranged by size:
630 331 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 301 984
Size: 301 683
Bsp1286I G_dGCh'C
Cuts at: 0 23 653 781 984
Size: 23 630 128 203
Fragments arranged by size:
630 203 128 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 674 984
Size: 674 310
BsrBI CCG'CTC
Cuts at: 0 425 430 516 984
Size: 425 5 86 468
Fragments arranged by size:
468 425 86 5
BsrFI r'CCGG_y
Cuts at: 0 452 984
Size: 452 532
BssSI C'ACGA_G
Cuts at: 0 172 357 652 984
Size: 172 185 295 332
Fragments arranged by size:
332 295 185 172
BstAPI GCAn_nnn'nTGC
Cuts at: 0 489 984
Size: 489 495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 131 984
Size: 131 853
EaeI y'GGCC_r
Cuts at: 0 224 500 620 984
Size: 224 276 120 364
Fragments arranged by size:
364 276 224 120
EagI C'GGCC_G
Cuts at: 0 224 984
Size: 224 760
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 145 490 984
Size: 145 345 494
Fragments arranged by size:
494 345 145
EcoICRI GAG'CTC
Cuts at: 0 651 984
Size: 651 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 127 984
Size: 37 90 857
Fragments arranged by size:
857 90 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 598 984
Size: 598 386
EcoO109I rG'GnC_Cy
Cuts at: 0 33 99 261 912 984
Size: 33 66 162 651 72
Fragments arranged by size:
651 162 72 66 33
HaeII r_GCGC'y
Cuts at: 0 241 319 453 594 723 984
Size: 241 78 134 141 129 261
Fragments arranged by size:
261 241 141 134 129 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 824 856 964 984
Size: 824 32 108 20
Fragments arranged by size:
824 108 32 20
KasI G'GCGC_C
Cuts at: 0 315 449 719 984
Size: 315 134 270 265
Fragments arranged by size:
315 270 265 134
MscI TGG'CCA
Cuts at: 0 622 984
Size: 622 362
MspA1I CmG'CkG
Cuts at: 0 489 984
Size: 489 495
NaeI GCC'GGC
Cuts at: 0 454 984
Size: 454 530
NarI GG'CG_CC
Cuts at: 0 316 450 720 984
Size: 316 134 270 264
Fragments arranged by size:
316 270 264 134
NgoMIV G'CCGG_C
Cuts at: 0 452 984
Size: 452 532
NheI G'CTAG_C
Cuts at: 0 234 984
Size: 234 750
NspI r_CATG'y
Cuts at: 0 528 984
Size: 528 456
PflMI CCAn_nnn'nTGG
Cuts at: 0 899 984
Size: 899 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 984
Size: 136 32 816
Fragments arranged by size:
816 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 99 912 984
Size: 99 813 72
Fragments arranged by size:
813 99 72
PstI C_TGCA'G
Cuts at: 0 212 485 984
Size: 212 273 499
Fragments arranged by size:
499 273 212
PvuII CAG'CTG
Cuts at: 0 489 984
Size: 489 495
SacI G_AGCT'C
Cuts at: 0 653 984
Size: 653 331
SanDI GG'GwC_CC
Cuts at: 0 99 984
Size: 99 885
ScaI AGT'ACT
Cuts at: 0 178 392 984
Size: 178 214 592
Fragments arranged by size:
592 214 178
SfcI C'TryA_G
Cuts at: 0 208 481 984
Size: 208 273 503
Fragments arranged by size:
503 273 208
SfoI GGC'GCC
Cuts at: 0 317 451 721 984
Size: 317 134 270 263
Fragments arranged by size:
317 270 263 134
SgrAI Cr'CCGG_yG
Cuts at: 0 452 984
Size: 452 532
SmaI CCC'GGG
Cuts at: 0 30 402 740 984
Size: 30 372 338 244
Fragments arranged by size:
372 338 244 30
SmlI C'TyrA_G
Cuts at: 0 865 984
Size: 865 119
SphI G_CATG'C
Cuts at: 0 528 984
Size: 528 456
SrfI GCCC'GGGC
Cuts at: 0 740 984
Size: 740 244
SspI AAT'ATT
Cuts at: 0 879 984
Size: 879 105
StyI C'CwwG_G
Cuts at: 0 599 984
Size: 599 385
TatI w'GTAC_w
Cuts at: 0 176 390 984
Size: 176 214 594
Fragments arranged by size:
594 214 176
XmaI C'CCGG_G
Cuts at: 0 28 400 738 984
Size: 28 372 338 246
Fragments arranged by size:
372 338 246 28
Enzymes that do cut:
AfeI AlwNI AvaI BanI BanII BbeI BbsI BbvCI
BglI Bme1580I BmtI BpmI Bpu10I BpuEI BsaHI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BtgZI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MspA1I NaeI
NarI NgoMIV NheI NspI PflMI PpiI PpuMI PstI
PvuII SacI SanDI ScaI SfcI SfoI SgrAI SmaI
SmlI SphI SrfI SspI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NcoI NdeI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI SexAI SfiI
SgfI SnaBI SpeI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmnI ZraI