Restriction map for
pFN21AE3907
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NcoI NdeI
NheI NotI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 280 GTCTAC GT^[AC][GT]AC
Alw44I 494 GTGCAC G^TGCAC
ApaI 539 GGGCCC GGGCC^C
ApaLI 494 GTGCAC G^TGCAC
Asp700I 240 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 145 GGTGCC G^G[CT][GA]CC
BcgI 1176 CGAGGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 438 GGATAC GTATCC(6/5)
BsaI 1022 GAGACC GGTCTC(1/5)
BsaWI 676 ACCGGA [AT]^CCGG[AT]
BsgI 577 CTGCAC GTGCAG(16/14)
Bsp1407I 569 TGTACA T^GTACA
BspMI 1025 ACCTGC ACCTGC(4/8)
BsrDI 1220 GCAATG GCAATG(2/0)
BsrGI 569 TGTACA T^GTACA
BsrI 149 CCAGT ACTGG(1/-1)
BstAPI 924 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 686 TTCGAA TT^CGAA
BstYI 8 AGATCC [GA]^GATC[CT]
DraIII 983 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1211 CCGTGG C^C[GA][CT]GG
EagI 358 CGGCCG C^GGCCG
EclXI 358 CGGCCG C^GGCCG
Eco52I 358 CGGCCG C^GGCCG
HaeII 975 GGCGCT [GA]GCGC^[CT]
HgaI 1239 GCGTC GACGC(5/10)
HinfI 943 GACTC G^A[ACGT]TC
MflI 8 AGATCC [GA]^GATC[CT]
MslI 48 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 1058 TCGCGA TCG^CGA
NspV 686 TTCGAA TT^CGAA
PleI 943 GACTC GAGTC(4/5)
PpuMI 167 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 586 CAGCTG CAG^CTG
SacI 832 GAGCTC GAGCT^C
SapI 756 GAAGAGC GCTCTTC(1/4)
ScaI 242 AGTACT AGT^ACT
SfaNI 47 GCATC GCATC(5/9)
SfuI 686 TTCGAA TT^CGAA
SmaI 844 CCCGGG CCC^GGG
SmlI 451 CTCAAG C^T[CT][GA]AG
SspBI 569 TGTACA T^GTACA
StuI 1049 AGGCCT AGG^CCT
Tsp509I 693 AATT ^AATT
XcmI 506 CCATCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 8 AGATCC [GA]^GATC[CT]
XmaCI 844 CCCGGG C^CCGGG
XmaI 844 CCCGGG C^CCGGG
XmnI 240 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspI 1072 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1096 GACGTGGTC
AvaI 844 CCCGGG C^[CT]CG[GA]G
1174 CCCGAG
BanII 539 GGGCCC G[GA]GC[CT]^C
832 GAGCTC
BsiEI 89 CGACCG CG[GA][CT]^CG
358 CGGCCG
BsmBI 1159 GAGACG CGTCTC(1/5)
1252 GAGACG
BsmFI 1151 GGGAC GGGAC(10/14)
609 GTCCC
BsoBI 844 CCCGGG C^[CT]CG[GA]G
1174 CCCGAG
BsrFI 360 GCCGGC [GA]^CCGG[CT]
701 GCCGGC
BstEII 414 GGTGACC G^GT[ACGT]ACC
888 GGTGACC
BstPI 414 GGTGACC G^GT[ACGT]ACC
888 GGTGACC
BstXI 125 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCAGCCGTGTGG
Cfr10I 360 GCCGGC [GA]^CCGG[CT]
701 GCCGGC
EarI 118 CTCTTC CTCTTC(1/4)
756 GAAGAG
EcoNI 51 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1026 CCTGCCCCAGG
EcoO65I 414 GGTGACC G^GT[ACGT]ACC
888 GGTGACC
EcoT14I 74 CCAAGG C^C[AT][AT]GG
1046 CCAAGG
Ksp632I 118 CTCTTC CTCTTC(1/4)
756 GAAGAG
NaeI 360 GCCGGC GCC^GGC
701 GCCGGC
NgoMIV 360 GCCGGC G^CCGGC
701 GCCGGC
PflFI 1072 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1096 GACGTGGTC
PflMI 192 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCACAGGATGG
StyI 74 CCAAGG C^C[AT][AT]GG
1046 CCAAGG
Tth111I 1072 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1096 GACGTGGTC
Van91I 192 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCACAGGATGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TseI Tsp45I
TspRI TthHB8I