(Circular) (Six-base) MAPSORT of: pFN21AE3907.seq Check: 4913 from: 1 to: 5968 pFN21AE3907 5968 bp With 182 enzymes: SgfI * March 28, 2012 11:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 3311 1937 Size: 349 1025 4594 Fragments arranged by size: 4594 1025 349 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 2584 2669 4031 5415 5683 1632 Size: 893 59 85 1362 1384 268 1917 Fragments arranged by size: 1917 1384 1362 893 268 85 59 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 4867 5844 1203 Size: 3664 977 1327 Fragments arranged by size: 3664 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AlwNI CAG_nnn'CTG Cuts at: 2203 2311 2935 3711 5283 2203 Size: 108 624 776 1572 2888 Fragments arranged by size: 2888 1572 776 624 108 ApaI G_GGCC'C Cuts at: 2548 2548 Size: 5968 ApaLI G'TGCA_C Cuts at: 2499 4025 5181 2499 Size: 1526 1156 3286 Fragments arranged by size: 3286 1526 1156 ApoI r'AATT_y Cuts at: 1493 3277 3503 3655 5764 1493 Size: 1784 226 152 2109 1697 Fragments arranged by size: 2109 1784 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 2849 3179 3293 1870 Size: 73 906 330 114 4545 Fragments arranged by size: 4545 906 330 114 73 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 3289 4744 618 Size: 325 217 627 363 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 363 325 217 BanII G_rGCy'C Cuts at: 1391 2548 2841 3287 1391 Size: 1157 293 446 4072 Fragments arranged by size: 4072 1157 446 293 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4997 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3170 3204 4159 4193 1903 Size: 34 1233 34 955 34 3678 Fragments arranged by size: 3678 1233 955 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2437 3860 5070 2437 Size: 1423 1210 3335 Fragments arranged by size: 3335 1423 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 3385 3385 Size: 5968 Bme1580I G_kGCm'C Cuts at: 1790 2503 2548 2880 2968 4029 5185 1790 Size: 713 45 332 88 1061 1156 2573 Fragments arranged by size: 2573 1156 1061 713 332 88 45 BmgBI CAC'GTC Cuts at: 1318 2022 3103 3265 1318 Size: 704 1081 162 4021 Fragments arranged by size: 4021 1081 704 162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3699 4657 442 Size: 416 53 83 399 24 2282 958 1753 Fragments arranged by size: 2282 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2249 3001 4628 1239 Size: 959 51 752 1627 2579 Fragments arranged by size: 2579 1627 959 752 51 Bpu10I CC'TnA_GC Cuts at: 2048 2048 Size: 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2441 4093 4958 5256 2441 Size: 1652 865 298 3153 Fragments arranged by size: 3153 1652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3021 4631 882 Size: 617 1522 1610 2219 Fragments arranged by size: 2219 1610 1522 617 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3775 4157 4792 275 Size: 53 83 186 936 2242 382 635 1451 Fragments arranged by size: 2242 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2681 4401 4799 5073 5220 5631 2681 Size: 1720 398 274 147 411 3018 Fragments arranged by size: 3018 1720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2558 2588 2852 Size: 30 165 30 708 30 417 30 264 Cuts at: 2852 2882 3116 3146 1178 Size: 30 234 30 4000 Fragments arranged by size: 4000 708 417 264 234 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2246 2783 2860 2932 1186 Size: 892 168 537 77 72 4222 Fragments arranged by size: 4222 892 537 168 77 72 BseYI C'CCAG_C Cuts at: 5171 5667 5171 Size: 496 5472 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2567 1163 Size: 573 831 4564 Fragments arranged by size: 4564 831 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2097 2366 3418 4179 5207 1540 Size: 275 186 96 269 1052 761 1028 2301 Fragments arranged by size: 2301 1052 1028 761 275 269 186 96 BsiHKAI G_wGCw'C Cuts at: 2115 2238 2503 2841 4029 4114 5185 2115 Size: 123 265 338 1188 85 1071 2898 Fragments arranged by size: 2898 1188 1071 338 265 123 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 BsmBI CGTCTCn'nnnn_ Cuts at: 3158 3251 3158 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2238 2503 2548 2841 2880 2968 Size: 399 325 123 265 45 293 39 88 Cuts at: 2968 3287 4029 4114 5185 1391 Size: 319 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 319 293 265 123 88 85 45 39 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3039 3323 844 Size: 2195 284 3489 Fragments arranged by size: 3489 2195 284 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3232 4463 4637 65 Size: 1183 1984 1231 174 1396 Fragments arranged by size: 1984 1396 1231 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2365 2706 4612 4799 1700 Size: 110 555 341 1906 187 2869 Fragments arranged by size: 2869 1906 555 341 187 110 BsrGI T'GTAC_A Cuts at: 96 2574 96 Size: 2478 3490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 4028 5040 4028 Size: 1012 4956 BstAPI GCAn_nnn'nTGC Cuts at: 794 2935 794 Size: 2141 3827 BstBI TT'CG_AA Cuts at: 2692 4772 2692 Size: 2080 3888 BstEII G'GTnAC_C Cuts at: 1184 2419 2893 1184 Size: 1235 474 4259 Fragments arranged by size: 4259 1235 474 BstXI CCAn_nnnn'nTGG Cuts at: 2137 3211 2137 Size: 1074 4894 BstYI r'GATC_y Cuts at: 1972 2013 3298 3665 4050 4067 5519 5605 5617 Size: 41 1285 367 385 17 1452 86 12 Cuts at: 5617 5963 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1285 385 367 346 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 3216 513 Size: 552 2151 3265 Fragments arranged by size: 3265 2151 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2248 487 Size: 683 56 1022 4207 Fragments arranged by size: 4207 1022 683 56 BtsI GCAGTG_nn' Cuts at: 2537 3086 3484 4278 4298 2537 Size: 549 398 794 20 4207 Fragments arranged by size: 4207 794 549 398 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DraIII CAC_nnn'GTG Cuts at: 2993 2993 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2363 2708 3213 Size: 54 1425 50 275 18 533 345 505 Cuts at: 3213 3415 4304 5933 8 Size: 202 889 1629 43 Fragments arranged by size: 1629 1425 889 533 505 345 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2363 3415 1537 Size: 275 551 1052 4090 Fragments arranged by size: 4090 1052 551 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2756 3898 1353 Size: 776 627 1142 3423 Fragments arranged by size: 3423 1142 776 627 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2620 3113 4555 4929 5075 387 Size: 1064 138 1031 493 1442 374 146 1280 Fragments arranged by size: 1442 1280 1064 1031 493 374 146 138 EcoICRI GAG'CTC Cuts at: 2839 2839 Size: 5968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2249 2525 2584 2669 3001 4031 Size: 393 566 51 276 59 85 332 1030 Cuts at: 4031 4628 5415 5683 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1030 787 597 566 393 332 276 268 85 59 51 EcoNI CCTnn'n_nnAGG Cuts at: 2060 3035 2060 Size: 975 4993 EcoO109I rG'GnC_Cy Cuts at: 2173 2341 2377 2544 3010 2173 Size: 168 36 167 466 5131 Fragments arranged by size: 5131 466 168 167 36 EcoRI G'AATT_C Cuts at: 3277 3655 3277 Size: 378 5590 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3076 3076 Size: 5968 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 2984 5115 1678 Size: 1306 2131 2531 Fragments arranged by size: 2531 2131 1306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3229 3261 4609 4641 4683 Size: 32 135 32 1657 32 1348 32 42 Cuts at: 4683 4715 1373 Size: 32 2626 Fragments arranged by size: 2626 1657 1348 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3312 3558 669 Size: 988 281 1374 246 3079 Fragments arranged by size: 3079 1374 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 2154 2301 5082 5266 1305 Size: 699 150 147 2781 184 2007 Fragments arranged by size: 2781 2007 699 184 150 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4146 Fragments arranged by size: 4146 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3926 4285 4444 518 Size: 648 891 1869 359 159 2042 Fragments arranged by size: 2042 1869 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 2416 2593 3384 4061 5209 5454 1606 Size: 579 231 177 791 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 677 579 245 231 177 NaeI GCC'GGC Cuts at: 2367 2708 2367 Size: 341 5627 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5416 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NgoMIV G'CCGG_C Cuts at: 2365 2706 2365 Size: 341 5627 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NruI TCG'CGA Cuts at: 3065 3065 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 3326 4871 1132 Size: 97 2097 1545 2229 Fragments arranged by size: 2229 2097 1545 97 PasI CC'CwG_GG Cuts at: 1348 2464 1348 Size: 1116 4852 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 2554 1108 Size: 1095 351 4522 Fragments arranged by size: 4522 1095 351 PfoI T'CCnGG_A Cuts at: 2136 2784 3153 2136 Size: 648 369 4951 Fragments arranged by size: 4951 648 369 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 2173 Size: 5968 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PspOMI G'GGCC_C Cuts at: 2544 2544 Size: 5968 PstI C_TGCA'G Cuts at: 830 2076 2307 3093 3120 3320 830 Size: 1246 231 786 27 200 3478 Fragments arranged by size: 3478 1246 786 231 200 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 PvuII CAG'CTG Cuts at: 2593 2593 Size: 5968 SacI G_AGCT'C Cuts at: 2841 2841 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SapI GCTCTTCn'nnn_ Cuts at: 2756 2756 Size: 5968 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 2249 4216 5928 2249 Size: 1967 1712 2289 Fragments arranged by size: 2289 1967 1712 SfcI C'TryA_G Cuts at: 826 1045 2072 2303 3089 3116 3316 4451 5132 Size: 219 1027 231 786 27 200 1135 681 Cuts at: 5132 5323 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1027 786 681 231 219 200 191 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 2851 3295 1872 Size: 979 444 4545 Fragments arranged by size: 4545 979 444 SmlI C'TyrA_G Cuts at: 820 1017 1943 2456 4072 4973 5235 820 Size: 197 926 513 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 513 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 3056 1517 Size: 1539 4429 StyI C'CwwG_G Cuts at: 513 1065 2079 3051 3407 513 Size: 552 1014 972 356 3074 Fragments arranged by size: 3074 1014 972 552 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 3120 3995 4012 4165 4350 4795 Size: 1255 558 982 875 17 153 185 445 Cuts at: 4795 325 Size: 1498 Fragments arranged by size: 1498 1255 982 875 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 2574 4214 Size: 274 80 33 51 1443 270 327 1640 Cuts at: 4214 5926 96 Size: 1712 138 Fragments arranged by size: 1712 1640 1443 327 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 3116 3148 5928 5960 2111 Size: 32 973 32 2780 32 2119 Fragments arranged by size: 2780 2119 973 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3080 3104 1316 Size: 1764 24 4180 Fragments arranged by size: 4180 1764 24 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 2518 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 2849 3293 1870 Size: 979 444 4545 Fragments arranged by size: 4545 979 444 XmnI GAAnn'nnTTC Cuts at: 2249 4097 2249 Size: 1848 4120 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI