Restriction map for
pFN21AA0627
(Linear) (Six-base) MAPSORT of: KIAA0627.seq Check: 4704 from: 1 to: 3882
KIAA0627.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 133 1123 1180 3882
Size: 133 990 57 2702
Fragments arranged by size:
2702 990 133 57
AccI GT'mk_AC
Cuts at: 0 1018 1740 3882
Size: 1018 722 2142
Fragments arranged by size:
2142 1018 722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 545 1280 2299 2822 2855 3882
Size: 358 187 735 1019 523 33 1027
Fragments arranged by size:
1027 1019 735 523 358 187 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3559 3882
Size: 3559 323
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 120 152 3882
Size: 120 32 3730
Fragments arranged by size:
3730 120 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1258 1786 3841 3882
Size: 1258 528 2055 41
Fragments arranged by size:
2055 1258 528 41
ApaLI G'TGCA_C
Cuts at: 0 3827 3882
Size: 3827 55
ApoI r'AATT_y
Cuts at: 0 290 344 700 785 2090 2439 2847 3882
Size: 290 54 356 85 1305 349 408 1035
Fragments arranged by size:
1305 1035 408 356 349 290 85 54
AseI AT'TA_AT
Cuts at: 0 3581 3882
Size: 3581 301
AvaI C'yCGr_G
Cuts at: 0 2468 3047 3882
Size: 2468 579 835
Fragments arranged by size:
2468 835 579
AvrII C'CTAG_G
Cuts at: 0 2022 3882
Size: 2022 1860
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 611 644 3882
Size: 611 33 3238
Fragments arranged by size:
3238 611 33
BamHI G'GATC_C
Cuts at: 0 2421 3882
Size: 2421 1461
BanI G'GyrC_C
Cuts at: 0 1135 1192 1675 3882
Size: 1135 57 483 2207
Fragments arranged by size:
2207 1135 483 57
BanII G_rGCy'C
Cuts at: 0 1432 3882
Size: 1432 2450
BbsI GAAGACnn'nnnn_
Cuts at: 0 901 1742 3714 3882
Size: 901 841 1972 168
Fragments arranged by size:
1972 901 841 168
BbvCI CC'TCA_GC
Cuts at: 0 803 1464 3488 3882
Size: 803 661 2024 394
Fragments arranged by size:
2024 803 661 394
BciVI GTATCCnnnnn_n'
Cuts at: 0 1221 3882
Size: 1221 2661
BclI T'GATC_A
Cuts at: 0 513 2346 3882
Size: 513 1833 1536
Fragments arranged by size:
1833 1536 513
BfrBI ATG'CAT
Cuts at: 0 1857 1885 3882
Size: 1857 28 1997
Fragments arranged by size:
1997 1857 28
BglII A'GATC_T
Cuts at: 0 3481 3882
Size: 3481 401
Bme1580I G_kGCm'C
Cuts at: 0 1197 1680 3831 3882
Size: 1197 483 2151 51
Fragments arranged by size:
2151 1197 483 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 3882
Size: 324 3558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 386 1373 1635 3863 3882
Size: 206 180 987 262 2228 19
Fragments arranged by size:
2228 987 262 206 180 19
Bpu10I CC'TnA_GC
Cuts at: 0 434 803 1182 1464 3488 3882
Size: 434 369 379 282 2024 394
Fragments arranged by size:
2024 434 394 379 369 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 2961 3091 3882
Size: 907 2054 130 791
Fragments arranged by size:
2054 907 791 130
BsaI GGTCTCn'nnnn_
Cuts at: 0 1391 3882
Size: 1391 2491
BsaBI GATnn'nnATC
Cuts at: 0 1842 3882
Size: 1842 2040
BsaHI Gr'CG_yC
Cuts at: 0 1871 3882
Size: 1871 2011
BsaWI w'CCGG_w
Cuts at: 0 103 3882
Size: 103 3779
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2745 2775 3882
Size: 2745 30 1107
Fragments arranged by size:
2745 1107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 699 1810 3024 3860 3882
Size: 699 1111 1214 836 22
Fragments arranged by size:
1214 1111 836 699 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 356 614 1208 3882
Size: 74 282 258 594 2674
Fragments arranged by size:
2674 594 282 258 74
BsiHKAI G_wGCw'C
Cuts at: 0 420 1432 3831 3882
Size: 420 1012 2399 51
Fragments arranged by size:
2399 1012 420 51
BsmI GAATG_Cn'
Cuts at: 0 1128 1206 1857 1859 3882
Size: 1128 78 651 2 2023
Fragments arranged by size:
2023 1128 651 78 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 681 3882
Size: 681 3201
Bsp1286I G_dGCh'C
Cuts at: 0 420 1197 1432 1680 3831 3882
Size: 420 777 235 248 2151 51
Fragments arranged by size:
2151 777 420 248 235 51
BspEI T'CCGG_A
Cuts at: 0 103 3882
Size: 103 3779
BspHI T'CATG_A
Cuts at: 0 789 3434 3882
Size: 789 2645 448
Fragments arranged by size:
2645 789 448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 133 1123 1180 1195 3653 3882
Size: 133 990 57 15 2458 229
Fragments arranged by size:
2458 990 229 133 57 15
BsrDI GCAATG_nn'
Cuts at: 0 2655 3007 3882
Size: 2655 352 875
Fragments arranged by size:
2655 875 352
BsrFI r'CCGG_y
Cuts at: 0 1376 3882
Size: 1376 2506
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1150 1297 1891 3882
Size: 1150 147 594 1991
Fragments arranged by size:
1991 1150 594 147
BstBI TT'CG_AA
Cuts at: 0 2637 3882
Size: 2637 1245
BstXI CCAn_nnnn'nTGG
Cuts at: 0 856 2418 2711 3882
Size: 856 1562 293 1171
Fragments arranged by size:
1562 1171 856 293
BstYI r'GATC_y
Cuts at: 0 438 445 2251 2421 3459 3481 3882
Size: 438 7 1806 170 1038 22 401
Fragments arranged by size:
1806 1038 438 401 170 22 7
Bsu36I CC'TnA_GG
Cuts at: 0 152 1707 3388 3882
Size: 152 1555 1681 494
Fragments arranged by size:
1681 1555 494 152
BtgI C'CryG_G
Cuts at: 0 1489 2666 3882
Size: 1489 1177 1216
Fragments arranged by size:
1489 1216 1177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1895 3882
Size: 1895 1987
BtsI GCAGTG_nn'
Cuts at: 0 352 942 2468 3538 3708 3882
Size: 352 590 1526 1070 170 174
Fragments arranged by size:
1526 1070 590 352 174 170
DraI TTT'AAA
Cuts at: 0 3411 3882
Size: 3411 471
DraIII CAC_nnn'GTG
Cuts at: 0 1108 3882
Size: 1108 2774
EaeI y'GGCC_r
Cuts at: 0 1266 2408 3509 3882
Size: 1266 1142 1101 373
Fragments arranged by size:
1266 1142 1101 373
EarI CTCTTCn'nnn_
Cuts at: 0 898 953 1041 1389 3882
Size: 898 55 88 348 2493
Fragments arranged by size:
2493 898 348 88 55
EcoICRI GAG'CTC
Cuts at: 0 1430 3882
Size: 1430 2452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 358 386 545 1280 1373 1635 2299
Size: 206 152 28 159 735 93 262 664
Cuts at: 2299 2822 2855 3863 3882
Size: 523 33 1008 19
Fragments arranged by size:
1008 735 664 523 262 206 159 152
93 33 28 19
EcoO109I rG'GnC_Cy
Cuts at: 0 965 1703 3882
Size: 965 738 2179
Fragments arranged by size:
2179 965 738
EcoRI G'AATT_C
Cuts at: 0 785 3882
Size: 785 3097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2203 2315 3882
Size: 2203 112 1567
Fragments arranged by size:
2203 1567 112
HaeII r_GCGC'y
Cuts at: 0 524 1182 3882
Size: 524 658 2700
Fragments arranged by size:
2700 658 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 430 462 952 984 1436 1468
Size: 22 32 376 32 490 32 452 32
Cuts at: 1468 3610 3642 3882
Size: 2142 32 240
Fragments arranged by size:
2142 490 452 376 240 32 32 32
32 32 22
HincII GTy'rAC
Cuts at: 0 1741 3781 3882
Size: 1741 2040 101
Fragments arranged by size:
2040 1741 101
HindIII A'AGCT_T
Cuts at: 0 21 215 464 2364 2617 3002 3713 3882
Size: 21 194 249 1900 253 385 711 169
Fragments arranged by size:
1900 711 385 253 249 194 169 21
MfeI C'AATT_G
Cuts at: 0 657 1995 3882
Size: 657 1338 1887
Fragments arranged by size:
1887 1338 657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 389 3882
Size: 138 251 3493
Fragments arranged by size:
3493 251 138
MscI TGG'CCA
Cuts at: 0 2410 3511 3882
Size: 2410 1101 371
Fragments arranged by size:
2410 1101 371
MslI CAynn'nnrTG
Cuts at: 0 623 1117 1862 2101 2180 3541 3882
Size: 623 494 745 239 79 1361 341
Fragments arranged by size:
1361 745 623 494 341 239 79
MspA1I CmG'CkG
Cuts at: 0 67 3882
Size: 67 3815
NcoI C'CATG_G
Cuts at: 0 2666 3882
Size: 2666 1216
NdeI CA'TA_TG
Cuts at: 0 1848 3882
Size: 1848 2034
NsiI A_TGCA'T
Cuts at: 0 1859 1887 3882
Size: 1859 28 1995
Fragments arranged by size:
1995 1859 28
NspI r_CATG'y
Cuts at: 0 754 2142 3882
Size: 754 1388 1740
Fragments arranged by size:
1740 1388 754
PflMI CCAn_nnn'nTGG
Cuts at: 0 1300 1720 3882
Size: 1300 420 2162
Fragments arranged by size:
2162 1300 420
PfoI T'CCnGG_A
Cuts at: 0 1083 1389 2424 3882
Size: 1083 306 1035 1458
Fragments arranged by size:
1458 1083 1035 306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 675 707 3882
Size: 675 32 3175
Fragments arranged by size:
3175 675 32
PpuMI rG'GwC_Cy
Cuts at: 0 965 3882
Size: 965 2917
PshAI GACnn'nnGTC
Cuts at: 0 1619 1746 2978 3882
Size: 1619 127 1232 904
Fragments arranged by size:
1619 1232 904 127
PsiI TTA'TAA
Cuts at: 0 211 2437 3882
Size: 211 2226 1445
Fragments arranged by size:
2226 1445 211
PstI C_TGCA'G
Cuts at: 0 1097 1134 2033 3570 3882
Size: 1097 37 899 1537 312
Fragments arranged by size:
1537 1097 899 312 37
PvuII CAG'CTG
Cuts at: 0 67 3882
Size: 67 3815
SacI G_AGCT'C
Cuts at: 0 1432 3882
Size: 1432 2450
SalI G'TCGA_C
Cuts at: 0 1739 3882
Size: 1739 2143
SfcI C'TryA_G
Cuts at: 0 476 1019 1093 1130 1503 1911 2029 3566
Size: 476 543 74 37 373 408 118 1537
Cuts at: 3566 3882
Size: 316
Fragments arranged by size:
1537 543 476 408 373 316 118 74
37
SmlI C'TyrA_G
Cuts at: 0 886 2976 3047 3106 3882
Size: 886 2090 71 59 776
Fragments arranged by size:
2090 886 776 71 59
SspI AAT'ATT
Cuts at: 0 2325 3308 3882
Size: 2325 983 574
Fragments arranged by size:
2325 983 574
StuI AGG'CCT
Cuts at: 0 2018 3882
Size: 2018 1864
StyI C'CwwG_G
Cuts at: 0 1139 1316 2022 2666 3629 3882
Size: 1139 177 706 644 963 253
Fragments arranged by size:
1139 963 706 644 253 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2224 3882
Size: 2224 1658
TatI w'GTAC_w
Cuts at: 0 2205 3882
Size: 2205 1677
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1151 1183 3702 3734 3882
Size: 1151 32 2519 32 148
Fragments arranged by size:
2519 1151 148 32 32
XbaI T'CTAG_A
Cuts at: 0 1327 3882
Size: 1327 2555
XhoI C'TCGA_G
Cuts at: 0 3047 3882
Size: 3047 835
XmnI GAAnn'nnTTC
Cuts at: 0 1857 3882
Size: 1857 2025
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaLI ApoI
AseI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BciVI BclI BfrBI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PflMI PfoI PpiI PpuMI PshAI PsiI PstI PvuII
SacI SalI SfcI SmlI SspI StuI StyI TaqII
TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI AscI AsiSI BbeI BcgI BglI BlpI
BmgBI BmtI BplI BsaAI BseYI BsiEI BsiWI BsrBI
BsrGI BssHII BssSI BstEII BstZ17I ClaI CspCI DrdI
EagI EciI EcoNI EcoRV FseI FspI FspAI HpaI
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XcmI XmaI ZraI