(Circular) (Six-base) MAPSORT of: pFN21ASDA0123.seq Check: 5700 from: 1 to: 6304 pFN21ASDA0123 6304 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3367 3367 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2378 3647 1937 Size: 441 1269 4594 Fragments arranged by size: 4594 1269 441 Acc65I G'GTAC_C Cuts at: 3625 3625 Size: 6304 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4367 5751 6019 1632 Size: 2735 1384 268 1917 Fragments arranged by size: 2735 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 2931 5203 6180 1203 Size: 1728 2272 977 1327 Fragments arranged by size: 2272 1728 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 3326 Size: 32 6272 AlwNI CAG_nnn'CTG Cuts at: 3382 4047 5619 3382 Size: 665 1572 4067 Fragments arranged by size: 4067 1572 665 ApaI G_GGCC'C Cuts at: 2954 3087 2954 Size: 133 6171 ApaLI G'TGCA_C Cuts at: 3418 4361 5517 3418 Size: 943 1156 4205 Fragments arranged by size: 4205 1156 943 ApoI r'AATT_y Cuts at: 1493 2295 2406 3613 3839 3991 6100 1493 Size: 802 111 1207 226 152 2109 1697 Fragments arranged by size: 2109 1697 1207 802 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2966 3629 1870 Size: 73 122 901 663 4545 Fragments arranged by size: 4545 901 663 122 73 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 3132 3567 3625 5080 Size: 325 217 627 345 1000 435 58 1455 Cuts at: 5080 618 Size: 1842 Fragments arranged by size: 1842 1455 1000 627 435 345 325 217 58 BanII G_rGCy'C Cuts at: 1391 2066 2954 2973 3087 3623 1391 Size: 675 888 19 114 536 4072 Fragments arranged by size: 4072 888 675 536 114 19 BbeI G_GCGC'C Cuts at: 3571 3571 Size: 6304 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5333 BbvCI CC'TCA_GC Cuts at: 2090 2090 Size: 6304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2035 2069 4495 4529 1903 Size: 34 98 34 2426 34 3678 Fragments arranged by size: 3678 2426 98 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2522 4915 136 Size: 107 122 71 631 605 850 2393 1525 Fragments arranged by size: 2393 1525 850 631 605 122 107 71 BglII A'GATC_T Cuts at: 2885 6299 2885 Size: 3414 2890 BlpI GC'TnA_GC Cuts at: 3721 3721 Size: 6304 Bme1580I G_kGCm'C Cuts at: 1790 2824 2954 3087 3422 4365 5521 1790 Size: 1034 130 133 335 943 1156 2573 Fragments arranged by size: 2573 1156 1034 943 335 133 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2890 2913 4035 Size: 416 53 83 399 24 1473 23 1122 Cuts at: 4035 4993 442 Size: 958 1753 Fragments arranged by size: 1753 1473 1122 958 416 399 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3019 3019 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 2618 3014 3032 3088 3379 4964 1239 Size: 951 428 396 18 56 291 1585 2579 Fragments arranged by size: 2579 1585 951 428 396 291 56 18 Bpu10I CC'TnA_GC Cuts at: 2090 2090 Size: 6304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4429 5294 5592 4429 Size: 865 298 5141 Fragments arranged by size: 5141 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4967 882 Size: 617 3468 2219 Fragments arranged by size: 3468 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3528 3601 4095 493 Size: 711 2324 73 494 2702 Fragments arranged by size: 2702 2324 711 494 73 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 4111 4493 5128 Size: 53 83 186 936 2035 543 382 635 Cuts at: 5128 275 Size: 1451 Fragments arranged by size: 2035 1451 936 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3594 4737 5135 5409 5556 5967 3594 Size: 1143 398 274 147 411 3931 Fragments arranged by size: 3931 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3140 3170 1178 Size: 30 165 30 1737 30 4312 Fragments arranged by size: 4312 1737 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3029 1186 Size: 1843 4461 BseYI C'CCAG_C Cuts at: 5507 6003 5507 Size: 496 5808 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5731 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2770 3754 4515 5543 1540 Size: 275 186 769 984 761 1028 2301 Fragments arranged by size: 2301 1028 984 769 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2802 2973 3126 3422 3537 4365 4450 5521 2802 Size: 171 153 296 115 828 85 1071 3585 Fragments arranged by size: 3585 1071 828 296 171 153 115 85 BsmI GAATG_Cn' Cuts at: 2737 3815 3908 2737 Size: 1078 93 5133 Fragments arranged by size: 5133 1078 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2802 2824 2954 2973 3087 3126 Size: 399 276 736 22 130 19 114 39 Cuts at: 3126 3422 3537 3623 4365 4450 5521 1391 Size: 296 115 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 736 399 296 276 130 115 114 86 85 39 22 19 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 2650 3327 4193 5923 2650 Size: 677 866 1730 3031 Fragments arranged by size: 3031 1730 866 677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2619 3367 3528 3659 844 Size: 1775 748 161 131 3489 Fragments arranged by size: 3489 1775 748 161 131 BsrBI CCG'CTC Cuts at: 1394 1823 4191 1394 Size: 429 2368 3507 Fragments arranged by size: 3507 2368 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2783 2848 3487 3585 4948 5135 1700 Size: 110 973 65 639 98 1363 187 2869 Fragments arranged by size: 2869 1363 973 639 187 110 98 65 BsrGI T'GTAC_A Cuts at: 96 2285 96 Size: 2189 4115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 2801 3410 4364 5376 2801 Size: 609 954 1012 3729 Fragments arranged by size: 3729 1012 954 609 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6304 BstBI TT'CG_AA Cuts at: 2007 5108 2007 Size: 3101 3203 BstEII G'GTnAC_C Cuts at: 1184 3506 1184 Size: 2322 3982 BstXI CCAn_nnnn'nTGG Cuts at: 2903 2903 Size: 6304 BstYI r'GATC_y Cuts at: 1972 2885 3634 4001 4386 4403 5855 5941 5953 Size: 913 749 367 385 17 1452 86 12 Cuts at: 5953 6299 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 913 749 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2747 2747 Size: 6304 BtgI C'CryG_G Cuts at: 513 1065 2022 2871 3276 513 Size: 552 957 849 405 3541 Fragments arranged by size: 3541 957 849 552 405 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 3042 3498 3820 4614 4634 3042 Size: 456 322 794 20 4712 Fragments arranged by size: 4712 794 456 322 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3550 5311 809 Size: 507 2234 1761 1802 Fragments arranged by size: 2234 1802 1761 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2781 3384 3495 Size: 54 1425 50 275 18 951 603 111 Cuts at: 3495 3751 4640 6269 8 Size: 256 889 1629 43 Fragments arranged by size: 1629 1425 951 889 603 275 256 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2161 3449 3596 4234 1353 Size: 808 1288 147 638 3423 Fragments arranged by size: 3423 1288 808 638 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3072 3285 4891 5265 5411 387 Size: 1064 138 1483 213 1606 374 146 1280 Fragments arranged by size: 1606 1483 1280 1064 374 213 146 138 EcoICRI GAG'CTC Cuts at: 2971 2971 Size: 6304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2618 3014 3032 3088 3379 4367 Size: 393 558 428 396 18 56 291 988 Cuts at: 4367 4964 5751 6019 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 988 787 597 558 428 396 393 291 268 56 18 EcoNI CCTnn'n_nnAGG Cuts at: 2746 3014 2746 Size: 268 6036 EcoO109I rG'GnC_Cy Cuts at: 2602 2602 Size: 6304 EcoRI G'AATT_C Cuts at: 2295 3613 3991 2295 Size: 1318 378 4608 Fragments arranged by size: 4608 1318 378 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 3210 3571 5451 1678 Size: 1532 361 1880 2531 Fragments arranged by size: 2531 1880 1532 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4945 4977 5019 5051 1373 Size: 32 135 32 3373 32 42 32 2626 Fragments arranged by size: 3373 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3648 3894 669 Size: 988 281 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KasI G'GCGC_C Cuts at: 3567 3567 Size: 6304 KpnI G_GTAC'C Cuts at: 3629 3629 Size: 6304 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2400 2968 5418 5602 1305 Size: 1095 568 2450 184 2007 Fragments arranged by size: 2450 2007 1095 568 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3386 10 Size: 54 1425 343 1554 2928 Fragments arranged by size: 2928 1554 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 2642 3050 4262 4621 4780 518 Size: 648 1302 174 408 1212 359 159 2042 Fragments arranged by size: 2042 1302 1212 648 408 359 174 159 MspA1I CmG'CkG Cuts at: 1606 3313 3720 4397 5545 5790 1606 Size: 1707 407 677 1148 245 2120 Fragments arranged by size: 2120 1707 1148 677 407 245 NaeI GCC'GGC Cuts at: 2785 2850 3489 2785 Size: 65 639 5600 Fragments arranged by size: 5600 639 65 NarI GG'CG_CC Cuts at: 3568 3568 Size: 6304 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4795 Fragments arranged by size: 4795 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NgoMIV G'CCGG_C Cuts at: 2783 2848 3487 2783 Size: 65 639 5600 Fragments arranged by size: 5600 639 65 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 2935 3100 3662 5207 1132 Size: 97 1706 165 562 1545 2229 Fragments arranged by size: 2229 1706 1545 562 165 97 PasI CC'CwG_GG Cuts at: 1348 3502 1348 Size: 2154 4150 PciI A'CATG_T Cuts at: 2931 5203 2931 Size: 2272 4032 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6304 PfoI T'CCnGG_A Cuts at: 3009 3009 Size: 6304 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2602 2602 Size: 6304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspOMI G'GGCC_C Cuts at: 2950 3083 2950 Size: 133 6171 PstI C_TGCA'G Cuts at: 830 2541 3656 830 Size: 1711 1115 3478 Fragments arranged by size: 3478 1711 1115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 3313 3313 Size: 6304 SacI G_AGCT'C Cuts at: 2973 2973 Size: 6304 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SapI GCTCTTCn'nnn_ Cuts at: 3596 3596 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2193 2537 3652 4787 5468 5659 826 Size: 219 1148 344 1115 1135 681 191 1471 Fragments arranged by size: 1471 1148 1135 1115 681 344 219 191 SfoI GGC'GCC Cuts at: 3569 3569 Size: 6304 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4408 5309 5571 820 Size: 197 926 2465 901 262 1553 Fragments arranged by size: 2465 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3601 4095 493 Size: 3108 494 2702 Fragments arranged by size: 3108 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 3349 1517 Size: 1832 4472 StyI C'CwwG_G Cuts at: 513 1065 2022 3743 513 Size: 552 957 1721 3074 Fragments arranged by size: 3074 1721 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 4331 4348 4501 4686 5131 325 Size: 1255 1914 837 17 153 185 445 1498 Fragments arranged by size: 1914 1498 1255 837 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2285 2898 4550 Size: 274 80 33 51 1443 308 613 1652 Cuts at: 4550 6262 96 Size: 1712 138 Fragments arranged by size: 1712 1652 1443 613 308 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6264 6296 6264 Size: 32 6272 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2944 2029 Size: 915 5389 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI