(Circular) (Six-base) MAPSORT of: pFN21AE0428.seq Check: 7629 from: 1 to: 5602 pFN21AE0428 5602 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2366 2945 1937 Size: 429 579 4594 Fragments arranged by size: 4594 579 429 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 2885 3665 5049 5317 1632 Size: 983 270 780 1384 268 1917 Fragments arranged by size: 1917 1384 983 780 270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 2222 2514 4501 5478 1203 Size: 1019 292 1987 977 1327 Fragments arranged by size: 1987 1327 1019 977 292 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 2779 3345 4917 2779 Size: 566 1572 3464 Fragments arranged by size: 3464 1572 566 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2399 2911 3137 3289 5398 1493 Size: 906 512 226 152 2109 1697 Fragments arranged by size: 2109 1697 906 512 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2154 2927 1870 Size: 73 211 773 4545 Fragments arranged by size: 4545 773 211 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2069 2102 2069 Size: 33 5569 BamHI G'GATC_C Cuts at: 2658 2932 3299 2658 Size: 274 367 4961 Fragments arranged by size: 4961 367 274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2829 2923 4378 618 Size: 325 217 627 1042 94 1455 1842 Fragments arranged by size: 1842 1455 1042 627 325 217 94 BanII G_rGCy'C Cuts at: 1391 2116 2921 1391 Size: 725 805 4072 Fragments arranged by size: 4072 805 725 BbeI G_GCGC'C Cuts at: 2833 2833 Size: 5602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2730 928 Size: 971 831 3800 Fragments arranged by size: 3800 971 831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 2671 1548 Size: 1123 4479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4213 136 Size: 107 122 71 631 605 2541 1525 Fragments arranged by size: 2541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 3663 4819 1790 Size: 1873 1156 2573 Fragments arranged by size: 2573 1873 1156 BmgBI CAC'GTC Cuts at: 1318 2464 1318 Size: 1146 4456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 3333 4291 Size: 416 53 83 399 24 708 1208 958 Cuts at: 4291 442 Size: 1753 Fragments arranged by size: 1753 1208 958 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2716 4262 1239 Size: 1477 1546 2579 Fragments arranged by size: 2579 1546 1477 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2225 3393 493 Size: 711 1021 1168 2702 Fragments arranged by size: 2702 1168 1021 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2830 3409 3791 4426 Size: 53 83 186 936 1297 579 382 635 Cuts at: 4426 275 Size: 1451 Fragments arranged by size: 1451 1297 936 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2712 4035 4433 4707 4854 5265 2712 Size: 1323 398 274 147 411 3049 Fragments arranged by size: 3049 1323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2768 2798 1178 Size: 30 165 30 1365 30 3982 Fragments arranged by size: 3982 1365 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 1186 Size: 1093 4509 BseYI C'CCAG_C Cuts at: 4805 5301 4805 Size: 496 5106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5029 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3663 3748 4819 3663 Size: 85 1071 4446 Fragments arranged by size: 4446 1071 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2921 3663 3748 4819 1391 Size: 399 326 805 742 85 1071 2174 Fragments arranged by size: 2174 1071 805 742 399 326 85 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 2613 3491 5221 2613 Size: 878 1730 2994 Fragments arranged by size: 2994 1730 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4097 4271 65 Size: 1183 2849 174 1396 Fragments arranged by size: 2849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2658 2800 2932 3299 3684 3701 5153 5239 Size: 686 142 132 367 385 17 1452 86 Cuts at: 5239 5251 5597 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 686 385 367 346 142 132 86 17 12 BstZ17I GTA'TAC Cuts at: 2367 2367 Size: 5602 Bsu36I CC'TnA_GG Cuts at: 2027 2027 Size: 5602 BtgI C'CryG_G Cuts at: 513 1065 2004 2301 513 Size: 552 939 297 3814 Fragments arranged by size: 3814 939 552 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3912 3932 3118 Size: 794 20 4788 Fragments arranged by size: 4788 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2682 3049 3938 Size: 54 1425 50 275 18 852 367 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 852 367 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3532 1353 Size: 2179 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2615 2716 2885 3665 4262 5049 5317 Size: 393 983 101 169 780 597 787 268 Cuts at: 5317 1239 Size: 1524 Fragments arranged by size: 1524 983 787 780 597 393 268 169 101 EcoNI CCTnn'n_nnAGG Cuts at: 2094 2094 Size: 5602 EcoO109I rG'GnC_Cy Cuts at: 2315 2315 Size: 5602 EcoRI G'AATT_C Cuts at: 2399 2911 3289 2399 Size: 512 378 4712 Fragments arranged by size: 4712 512 378 FspI TGC'GCA Cuts at: 2360 4108 2360 Size: 1748 3854 HaeII r_GCGC'y Cuts at: 1678 2833 4749 1678 Size: 1155 1916 2531 Fragments arranged by size: 2531 1916 1155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373 Size: 32 135 32 2671 32 42 32 2626 Fragments arranged by size: 2671 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 2228 748 Size: 1480 4122 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KasI G'GCGC_C Cuts at: 2829 2829 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2903 4716 4900 1305 Size: 1598 1813 184 2007 Fragments arranged by size: 2007 1813 1598 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3780 Fragments arranged by size: 3780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2628 3560 3919 4078 518 Size: 648 1462 932 359 159 2042 Fragments arranged by size: 2042 1462 932 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2687 3018 3695 4843 5088 1606 Size: 594 487 331 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 487 331 245 NarI GG'CG_CC Cuts at: 2830 2830 Size: 5602 NcoI C'CATG_G Cuts at: 513 1065 2004 2301 513 Size: 552 939 297 3814 Fragments arranged by size: 3814 939 552 297 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2163 2365 2518 2960 4505 1132 Size: 97 934 202 153 442 1545 2229 Fragments arranged by size: 2229 1545 934 442 202 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 2514 4501 2514 Size: 1987 3615 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5602 PfoI T'CCnGG_A Cuts at: 2182 2182 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 2315 Size: 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 2450 3172 2450 Size: 722 4880 PstI C_TGCA'G Cuts at: 830 2954 830 Size: 2124 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2200 2200 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2950 4085 4766 4957 826 Size: 219 1905 1135 681 191 1471 Fragments arranged by size: 1905 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2831 2831 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 2095 2301 3041 513 Size: 552 939 91 206 740 3074 Fragments arranged by size: 3074 939 740 552 206 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 3848 5560 Size: 274 80 33 51 1443 534 1337 1712 Cuts at: 5560 96 Size: 138 Fragments arranged by size: 1712 1443 1337 534 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 2178 2403 3731 2178 Size: 225 1328 4049 Fragments arranged by size: 4049 1328 225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI