(Circular) (Six-base) MAPSORT of: pFN21AB0946.seq Check: 6624 from: 1 to: 5902 pFN21AB0946 5902 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2399 3245 1937 Size: 462 846 4594 Fragments arranged by size: 4594 846 462 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2451 3201 3965 5349 5617 1632 Size: 819 750 764 1384 268 1917 Fragments arranged by size: 1917 1384 819 764 750 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3645 5217 3645 Size: 1572 4330 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2327 2335 2347 2437 2467 2527 2667 2919 Size: 834 8 12 90 30 60 140 252 Cuts at: 2919 3087 3211 3437 3589 5698 1493 Size: 168 124 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 252 226 168 152 140 124 90 60 30 12 8 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 3227 1870 Size: 73 1284 4545 Fragments arranged by size: 4545 1284 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 2190 3221 1391 Size: 799 1031 4072 Fragments arranged by size: 4072 1031 799 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2717 928 Size: 971 818 4113 Fragments arranged by size: 4113 971 818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 2160 1548 Size: 612 5290 BfrBI ATG'CAT Cuts at: 2781 2781 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 3963 5119 1790 Size: 2173 1156 2573 Fragments arranged by size: 2573 2173 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3633 4591 442 Size: 416 53 83 399 24 2216 958 1753 Fragments arranged by size: 2216 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2583 2583 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2683 3033 4562 1239 Size: 1444 350 1529 2579 Fragments arranged by size: 2579 1529 1444 350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3113 4027 4892 5190 3113 Size: 914 865 298 3825 Fragments arranged by size: 3825 914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2131 4565 882 Size: 617 632 2434 2219 Fragments arranged by size: 2434 2219 632 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5902 BseYI C'CCAG_C Cuts at: 5105 5601 5105 Size: 496 5406 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2837 3963 4048 5119 2837 Size: 1126 85 1071 3620 Fragments arranged by size: 3620 1126 1071 85 BsmI GAATG_Cn' Cuts at: 2369 3119 3413 3506 2369 Size: 750 294 93 4765 Fragments arranged by size: 4765 750 294 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2988 2988 Size: 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2837 3221 3963 4048 5119 1391 Size: 399 400 647 384 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 647 400 399 384 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 2047 3791 5521 2047 Size: 1744 1730 2428 Fragments arranged by size: 2428 1744 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3257 844 Size: 2413 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4546 4733 1700 Size: 110 2736 187 2869 Fragments arranged by size: 2869 2736 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897 Size: 1260 367 385 17 1452 86 12 346 Cuts at: 5897 1972 Size: 1977 Fragments arranged by size: 1977 1452 1260 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2722 2806 513 Size: 552 1657 84 3609 Fragments arranged by size: 3609 1657 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2620 3418 4212 4232 2620 Size: 798 794 20 4290 Fragments arranged by size: 4290 798 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 2526 3206 2526 Size: 680 5222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867 Size: 54 1425 50 275 18 1519 889 1629 Cuts at: 5867 8 Size: 43 Fragments arranged by size: 1629 1519 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 3832 1353 Size: 693 1786 3423 Fragments arranged by size: 3423 1786 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2683 3033 3201 3965 4562 5349 Size: 393 819 232 350 168 764 597 787 Cuts at: 5349 5617 1239 Size: 268 1524 Fragments arranged by size: 1524 819 787 764 597 393 350 268 232 168 EcoRI G'AATT_C Cuts at: 2437 2667 2919 3087 3211 3589 2437 Size: 230 252 168 124 378 4750 Fragments arranged by size: 4750 378 252 230 168 124 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 5049 1678 Size: 3371 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 2586 2618 4543 Size: 32 135 32 638 32 344 32 1925 Cuts at: 4543 4575 4617 4649 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1925 638 344 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 3048 748 Size: 2300 3602 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2448 3860 4219 4378 518 Size: 648 1282 1412 359 159 2042 Fragments arranged by size: 2042 1412 1282 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3318 3995 5143 5388 1606 Size: 1712 677 1148 245 2120 Fragments arranged by size: 2120 1712 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2722 2806 513 Size: 552 1657 84 3609 Fragments arranged by size: 3609 1657 552 84 NdeI CA'TA_TG Cuts at: 387 2155 387 Size: 1768 4134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NsiI A_TGCA'T Cuts at: 2783 2783 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2064 2613 3260 4805 1132 Size: 97 835 549 647 1545 2229 Fragments arranged by size: 2229 1545 835 647 549 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 3971 4003 5484 5516 2111 Size: 32 1828 32 1481 32 2497 Fragments arranged by size: 2497 1828 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 2489 3472 2489 Size: 983 4919 PstI C_TGCA'G Cuts at: 830 3254 830 Size: 2424 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 5902 SfcI C'TryA_G Cuts at: 826 1045 2034 2130 3058 3250 4385 5066 5257 Size: 219 989 96 928 192 1135 681 191 Cuts at: 5257 826 Size: 1471 Fragments arranged by size: 1471 1135 989 928 681 219 192 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3128 4006 4907 5169 820 Size: 197 926 1185 878 901 262 1553 Fragments arranged by size: 1553 1185 926 901 878 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 2064 3260 1132 Size: 932 1196 3774 Fragments arranged by size: 3774 1196 932 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 2798 2966 1517 Size: 1281 168 4453 Fragments arranged by size: 4453 1281 168 StyI C'CwwG_G Cuts at: 513 1065 2722 2806 3341 513 Size: 552 1657 84 535 3074 Fragments arranged by size: 3074 1657 552 535 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3929 3946 4099 4284 4729 325 Size: 1255 2349 17 153 185 445 1498 Fragments arranged by size: 2349 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 3050 4031 3050 Size: 981 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI