(Circular) (Six-base) MAPSORT of: pFN21AB9665.seq Check: 7005 from: 1 to: 5974 pFN21AB9665 5974 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2259 2679 3016 4037 5421 5689 1632 Size: 375 252 420 337 1021 1384 268 1917 Fragments arranged by size: 1917 1384 1021 420 375 337 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 3038 4873 5850 1203 Size: 1835 1835 977 1327 Fragments arranged by size: 1835 1835 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2654 2991 1166 Size: 1488 337 4149 Fragments arranged by size: 4149 1488 337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 3717 5289 3717 Size: 1572 4402 ApaLI G'TGCA_C Cuts at: 2758 4031 5187 2758 Size: 1273 1156 3545 Fragments arranged by size: 3545 1273 1156 ApoI r'AATT_y Cuts at: 1493 2115 2309 2393 2729 3283 3509 3661 5770 Size: 622 194 84 336 554 226 152 2109 Cuts at: 5770 1493 Size: 1697 Fragments arranged by size: 2109 1697 622 554 336 226 194 152 84 AseI AT'TA_AT Cuts at: 160 1032 2297 4528 160 Size: 872 1265 2231 1606 Fragments arranged by size: 2231 1606 1265 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BamHI G'GATC_C Cuts at: 2141 3304 3671 2141 Size: 1163 367 4444 Fragments arranged by size: 4444 1163 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2035 2203 2455 3293 1391 Size: 644 168 252 838 4072 Fragments arranged by size: 4072 838 644 252 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3171 928 Size: 971 1272 3731 Fragments arranged by size: 3731 1272 971 BbvCI CC'TCA_GC Cuts at: 3075 3075 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 2256 1548 Size: 708 5266 BfrBI ATG'CAT Cuts at: 2503 2587 3017 2503 Size: 84 430 5460 Fragments arranged by size: 5460 430 84 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2037 2289 2373 2541 2787 5969 2037 Size: 252 84 168 246 3182 2042 Fragments arranged by size: 3182 2042 252 246 168 84 BlpI GC'TnA_GC Cuts at: 2875 3391 2875 Size: 516 5458 Bme1580I G_kGCm'C Cuts at: 1790 2762 4035 5191 1790 Size: 972 1273 1156 2573 Fragments arranged by size: 2573 1273 1156 972 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 2832 2916 Size: 416 53 83 399 24 658 757 84 Cuts at: 2916 3705 4663 442 Size: 789 958 1753 Fragments arranged by size: 1753 958 789 757 658 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2255 2339 2507 2591 2759 4634 1239 Size: 932 84 84 168 84 168 1875 2579 Fragments arranged by size: 2579 1875 932 168 168 84 84 84 Bpu10I CC'TnA_GC Cuts at: 3075 3075 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3092 1186 Size: 1906 4068 BseYI C'CCAG_C Cuts at: 5177 5673 5177 Size: 496 5478 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2788 2872 3124 1163 Size: 573 1052 84 252 4013 Fragments arranged by size: 4013 1052 573 252 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2035 2203 2455 2762 2876 4035 4120 5191 2035 Size: 168 252 307 114 1159 85 1071 2818 Fragments arranged by size: 2818 1159 1071 307 252 168 114 85 BsmI GAATG_Cn' Cuts at: 3265 3485 3578 3265 Size: 220 93 5661 Fragments arranged by size: 5661 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2203 2455 2762 2876 3293 4035 Size: 399 245 168 252 307 114 417 742 Cuts at: 4035 4120 5191 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 417 399 307 252 245 168 114 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3142 3863 5593 3142 Size: 721 1730 3523 Fragments arranged by size: 3523 1730 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2670 4469 4643 65 Size: 1183 1422 1799 174 1396 Fragments arranged by size: 1799 1422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 3206 96 Size: 3110 2864 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 2766 2850 794 Size: 1972 84 3918 Fragments arranged by size: 3918 1972 84 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2037 2141 2289 2373 2541 2787 3304 3671 Size: 65 104 148 84 168 246 517 367 Cuts at: 3671 4056 4073 5525 5611 5623 5969 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 517 385 367 346 246 168 148 104 86 84 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5422 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3490 4284 4304 2851 Size: 639 794 20 4521 Fragments arranged by size: 4521 794 639 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2766 2766 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3904 1353 Size: 2551 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2033 2201 2453 2033 Size: 168 252 5554 Fragments arranged by size: 5554 252 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2171 2255 2259 2339 2507 2591 Size: 393 375 164 84 4 80 168 84 Cuts at: 2591 2679 2759 3016 4037 4634 5421 5689 1239 Size: 88 80 257 1021 597 787 268 1524 Fragments arranged by size: 1524 1021 787 597 393 375 268 257 168 164 88 84 84 80 80 4 EcoNI CCTnn'n_nnAGG Cuts at: 2277 2277 Size: 5974 EcoRI G'AATT_C Cuts at: 2115 2309 2393 2729 3283 3661 2115 Size: 194 84 336 554 378 4428 Fragments arranged by size: 4428 554 378 336 194 84 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 4615 4647 4689 Size: 32 135 32 450 32 2561 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 2561 450 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 2186 2523 2944 748 Size: 1438 337 421 3778 Fragments arranged by size: 3778 1438 421 337 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 5088 5272 1305 Size: 1175 2608 184 2007 Fragments arranged by size: 2608 2007 1175 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2082 2402 2586 2654 2839 2991 Size: 648 843 73 320 184 68 185 152 Cuts at: 2991 3932 4291 4450 518 Size: 941 359 159 2042 Fragments arranged by size: 2042 941 843 648 359 320 185 184 159 152 73 68 MspA1I CmG'CkG Cuts at: 1606 3390 4067 5215 5460 1606 Size: 1784 677 1148 245 2120 Fragments arranged by size: 2120 1784 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5422 NdeI CA'TA_TG Cuts at: 387 2583 387 Size: 2196 3778 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NsiI A_TGCA'T Cuts at: 2505 2589 3019 2505 Size: 84 430 5460 Fragments arranged by size: 5460 430 84 NspI r_CATG'y Cuts at: 1132 1229 2507 2591 3021 3042 3332 4877 1132 Size: 97 1278 84 430 21 290 1545 2229 Fragments arranged by size: 2229 1545 1278 430 290 97 84 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 3038 4873 3038 Size: 1835 4139 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 SacI G_AGCT'C Cuts at: 2035 2203 2455 2035 Size: 168 252 5554 Fragments arranged by size: 5554 252 168 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 3322 4457 5138 5329 826 Size: 219 2277 1135 681 191 1471 Fragments arranged by size: 2277 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4078 4979 5241 820 Size: 197 926 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 3413 513 Size: 552 2348 3074 Fragments arranged by size: 3074 2348 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4001 4018 4171 4356 4801 325 Size: 1255 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2515 2646 3041 Size: 274 80 33 51 1443 538 131 395 Cuts at: 3041 3206 4220 5932 96 Size: 165 1014 1712 138 Fragments arranged by size: 1712 1443 1014 538 395 274 165 138 131 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 2020 2692 2861 3029 4103 2020 Size: 672 169 168 1074 3891 Fragments arranged by size: 3891 1074 672 169 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI