################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BbrPI BclI BfrI BlnI BlpI Bpu1102I BsaHI BsiWI BsmBI BsmI BspDI BspHI BsrDI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoRV FbaI FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tru9I XbaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### ApaI 52 GGGCCC GGGCC^C Asp700I 1215 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 280 GGCACC G^G[CT][GA]CC BciVI 125 GTATCC GTATCC(6/5) BglII 2000 AGATCT A^GATCT BsaAI 1336 CACGTA [CT]AC^GT[GA] BssSI 391 CTCGTG CACGAG(-5/-1) DraIII 1417 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 243 GACCCGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2528 CGGCCG C^GGCCG EclXI 2528 CGGCCG C^GGCCG Eco52I 2528 CGGCCG C^GGCCG EcoNI 20 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2401 GAATTC G^AATTC EcoT22I 1024 ATGCAT ATGCA^T NsiI 1024 ATGCAT ATGCA^T PaeR7I 1504 CTCGAG C^TCGAG PshAI 715 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 842 ACCAGGT A^CC[AT]GGT SfiI 2503 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1504 CTCGAG C^T[CT][GA]AG SphI 2493 GCATGC GCATG^C XhoI 1504 CTCGAG C^TCGAG XmnI 1215 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 1219 AAATTC [GA]^AATT[CT] 2401 GAATTC ApoI 1219 AAATTC [GA]^AATT[CT] 2401 GAATTC BbvCI 1572 CCTCAGC CCTCAGC(-5/-2) 1773 CCTCAGC BcgI 314 GCAGGCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 744 GCAGACCTATCG BfaI 1360 CTAG C^TAG 1717 CTAG BsaBI 1420 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1605 GATGGTCATC Bsp1407I 1184 TGTACA T^GTACA 1484 TGTACA BspLU11I 464 ACATGT A^CATGT 1634 ACATGT BsrGI 1184 TGTACA T^GTACA 1484 TGTACA BssHII 159 GCGCGC G^CGCGC 198 GCGCGC BstEII 498 GGTCACC G^GT[ACGT]ACC 1849 GGTGACC BstPI 498 GGTCACC G^GT[ACGT]ACC 1849 GGTGACC BstXI 270 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1787 CCAAGCCGCTGG BstYI 896 AGATCC [GA]^GATC[CT] 2000 AGATCT EcoO65I 498 GGTCACC G^GT[ACGT]ACC 1849 GGTGACC MaeI 1360 CTAG C^TAG 1717 CTAG MamI 1420 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1605 GATGGTCATC MflI 896 AGATCC [GA]^GATC[CT] 2000 AGATCT PstI 1324 CTGCAG CTGCA^G 1540 CTGCAG PvuII 730 CAGCTG CAG^CTG 1313 CAGCTG SacI 805 GAGCTC GAGCT^C 1087 GAGCTC SapI 1084 GAAGAGC GCTCTTC(1/4) 2443 GCTCTTC SspBI 1184 TGTACA T^GTACA 1484 TGTACA TfiI 1386 GATTC G^A[AT]TC 1509 GAATC XhoII 896 AGATCC [GA]^GATC[CT] 2000 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AccIII AciI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI AspHI AspI AvaI AvaII AviII BalI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XmaCI XmaI