Restriction map for
pFN21AE3434
(Circular) (Six-base) MAPSORT of: pFN21AE3434.seq Check: 2339 from: 1 to: 7363
pFN21AE3434 7363 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3188 3188
Size: 7363
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5173 278
Size: 53 83 186 936 3637 2468
Fragments arranged by size:
3637 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4706 1937
Size: 2769 4594
Acc65I G'GTAC_C
Cuts at: 4684 4684
Size: 7363
AclI AA'CG_TT
Cuts at: 1563 5490 5863 1563
Size: 3927 373 3063
Fragments arranged by size:
3927 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2544 3239 3692 5426 6810 7078 1632
Size: 470 442 695 453 1734 1384 268 1917
Fragments arranged by size:
1917 1734 1384 695 470 453 442 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7166
AflIII A'CryG_T
Cuts at: 1203 2469 2681 3639 6262 7239 1203
Size: 1266 212 958 2623 977 1327
Fragments arranged by size:
2623 1327 1266 977 958 212
AgeI A'CCGG_T
Cuts at: 6194 6194
Size: 7363
AhdI GACnn_n'nnGTC
Cuts at: 6092 6092
Size: 7363
AleI CACnn'nnGTG
Cuts at: 1166 3248 1166
Size: 2082 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4581 1184
Size: 3397 3966
AlwNI CAG_nnn'CTG
Cuts at: 2101 2557 4587 4631 5106 6678 2101
Size: 456 2030 44 475 1572 2786
Fragments arranged by size:
2786 2030 1572 475 456 44
ApaI G_GGCC'C
Cuts at: 2061 2061
Size: 7363
ApaLI G'TGCA_C
Cuts at: 3764 3953 4186 4368 5420 6576 3764
Size: 189 233 182 1052 1156 4551
Fragments arranged by size:
4551 1156 1052 233 189 182
ApoI r'AATT_y
Cuts at: 1493 3224 4406 4672 4898 5050 7159 1493
Size: 1731 1182 266 226 152 2109 1697
Fragments arranged by size:
2109 1731 1697 1182 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5917 160
Size: 872 4885 1606
Fragments arranged by size:
4885 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7363
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2061 2697 3509 4076 4398 4504
Size: 73 111 7 636 812 567 322 106
Cuts at: 4504 4513 4688 1870
Size: 9 175 4545
Fragments arranged by size:
4545 812 636 567 322 175 111 106
73 9 7
BamHI G'GATC_C
Cuts at: 4693 5060 4693
Size: 367 6996
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2285 4684 6139 618
Size: 325 217 627 498 2399 1455 1842
Fragments arranged by size:
2399 1842 1455 627 498 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2390 2814 3096 4064 4682 1391
Size: 670 329 424 282 968 618 4072
Fragments arranged by size:
4072 968 670 618 424 329 282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2532 3083 3680 928
Size: 971 633 551 597 4611
Fragments arranged by size:
4611 971 633 597 551
BbvCI CC'TCA_GC
Cuts at: 3578 3779 3578
Size: 201 7162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2308 2342 2738 2772 5554 5588 1903
Size: 34 371 34 396 34 2782 34 3678
Fragments arranged by size:
3678 2782 396 371 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2141 5255 6465 2141
Size: 3114 1210 3039
Fragments arranged by size:
3114 3039 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7363
BfrBI ATG'CAT
Cuts at: 3031 3031
Size: 7363
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2191 2414 4199
Size: 107 122 71 631 605 519 223 1785
Cuts at: 4199 4262 4515 4574 5974 136
Size: 63 253 59 1400 1525
Fragments arranged by size:
1785 1525 1400 631 605 519 253 223
122 107 71 63 59
BglII A'GATC_T
Cuts at: 4005 7358 4005
Size: 3353 4010
BlpI GC'TnA_GC
Cuts at: 4780 4780
Size: 7363
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2610 3768 3957 4190 4372 5424 6580
Size: 271 549 1158 189 233 182 1052 1156
Cuts at: 6580 1790
Size: 2573
Fragments arranged by size:
2573 1158 1156 1052 549 271 233 189
182
BmgBI CAC'GTC
Cuts at: 1318 3292 3325 1318
Size: 1974 33 5356
Fragments arranged by size:
5356 1974 33
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2610 5094 6052
Size: 416 53 83 399 24 1193 2484 958
Cuts at: 6052 442
Size: 1753
Fragments arranged by size:
2484 1753 1193 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2058 2794 4083 2058
Size: 736 1289 5338
Fragments arranged by size:
5338 1289 736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 2750 2759 3131 3183 3290 3407 3821
Size: 1490 21 9 372 52 107 117 414
Cuts at: 3821 4163 4334 4346 6023 1239
Size: 342 171 12 1677 2579
Fragments arranged by size:
2579 1677 1490 414 372 342 171 117
107 52 21 12 9
Bpu10I CC'TnA_GC
Cuts at: 2550 3578 3706 3779 2550
Size: 1028 128 73 6134
Fragments arranged by size:
6134 1028 128 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5488 6353 6651 5488
Size: 865 298 6200
Fragments arranged by size:
6200 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2735 2857 3448 3680 3962 4647 6026
Size: 617 1236 122 591 232 282 685 1379
Cuts at: 6026 882
Size: 2219
Fragments arranged by size:
2219 1379 1236 685 617 591 282 232
122
BsaAI yAC'GTr
Cuts at: 493 1204 3343 5154 493
Size: 711 2139 1811 2702
Fragments arranged by size:
2702 2139 1811 711
BsaBI GATnn'nnATC
Cuts at: 3429 3614 7357 3429
Size: 185 3743 3435
Fragments arranged by size:
3743 3435 185
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5170 5552 6187 275
Size: 53 83 186 936 3637 382 635 1451
Fragments arranged by size:
3637 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2568 2840 3753 4381 5796 6194 6468 6615 7026
Size: 272 913 628 1415 398 274 147 411
Cuts at: 7026 2568
Size: 2905
Fragments arranged by size:
2905 1415 913 628 411 398 274 272
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2338 2368 2563 2593 3896
Size: 30 165 30 935 30 195 30 1303
Cuts at: 3896 3926 4319 4349 1178
Size: 30 393 30 4192
Fragments arranged by size:
4192 1303 935 393 195 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 4094 4097 4160 4169 1186
Size: 1540 1368 3 63 9 4380
Fragments arranged by size:
4380 1540 1368 63 9 3
BseYI C'CCAG_C
Cuts at: 2635 2961 4624 6566 7062 2635
Size: 326 1663 1942 496 2936
Fragments arranged by size:
2936 1942 1663 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 3296 3345 1163
Size: 573 651 909 49 5181
Fragments arranged by size:
5181 909 651 573 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2230 3739 4536 4813 5574
Size: 275 186 18 211 1509 797 277 761
Cuts at: 5574 6602 1540
Size: 1028 2301
Fragments arranged by size:
2301 1509 1028 797 761 277 275 211
186 18
BsiHKAI G_wGCw'C
Cuts at: 2814 3096 3510 3768 3957 4190 4372 4560 5424
Size: 282 414 258 189 233 182 188 864
Cuts at: 5424 5509 6580 2814
Size: 85 1071 3597
Fragments arranged by size:
3597 1071 864 414 282 258 233 189
188 182 85
BsmI GAATG_Cn'
Cuts at: 4874 4967 4874
Size: 93 7270
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2390 2610 2814 3096 3510 3768
Size: 399 271 329 220 204 282 414 258
Cuts at: 3768 3957 4064 4190 4372 4560 4682 5424 5509
Size: 189 107 126 182 188 122 742 85
Cuts at: 5509 6580 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 329 282 271
258 220 204 189 188 182 126 122
107 85
BspEI T'CCGG_A
Cuts at: 2568 2840 3753 4381 7026 2568
Size: 272 913 628 2645 2905
Fragments arranged by size:
2905 2645 913 628 272
BspHI T'CATG_A
Cuts at: 5252 6982 5252
Size: 1730 5633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2826 3188 3222 4718 844
Size: 1982 362 34 1496 3489
Fragments arranged by size:
3489 1982 1496 362 34
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 2179 2451 3171 3208 5250 1394
Size: 429 350 6 272 720 37 2042 3507
Fragments arranged by size:
3507 2042 720 429 350 272 37 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 5858 6032 65
Size: 1183 4610 174 1396
Fragments arranged by size:
4610 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 2229 2288 6007 6194 1700
Size: 110 380 39 59 3719 187 2869
Fragments arranged by size:
3719 2869 380 187 110 59 39
BsrGI T'GTAC_A
Cuts at: 96 3189 3489 96
Size: 3093 300 3970
Fragments arranged by size:
3970 3093 300
BssHII G'CGCG_C
Cuts at: 1925 2164 2203 1925
Size: 239 39 7085
Fragments arranged by size:
7085 239 39
BssSI C'ACGA_G
Cuts at: 2396 5423 6435 2396
Size: 3027 1012 3324
Fragments arranged by size:
3324 3027 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3368 4208 4587 794
Size: 2574 840 379 3570
Fragments arranged by size:
3570 2574 840 379
BstBI TT'CG_AA
Cuts at: 6167 6167
Size: 7363
BstEII G'GTnAC_C
Cuts at: 1184 2503 3854 1184
Size: 1319 1351 4693
Fragments arranged by size:
4693 1351 1319
BstXI CCAn_nnnn'nTGG
Cuts at: 2282 3799 2282
Size: 1517 5846
BstYI r'GATC_y
Cuts at: 1972 2901 4005 4693 5060 5445 5462 6914 7000
Size: 929 1104 688 367 385 17 1452 86
Cuts at: 7000 7012 7358 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1104 929 688 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2214 2275 3528 3633 3678 3915
Size: 552 939 210 61 1253 105 45 237
Cuts at: 3915 4527 513
Size: 612 3349
Fragments arranged by size:
3349 1253 939 612 552 237 210 105
61 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6624
Fragments arranged by size:
6624 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 3378 3906 4338 4372 4588 4879 5673 5693
Size: 1330 528 432 34 216 291 794 20
Cuts at: 5693 2048
Size: 3718
Fragments arranged by size:
3718 1330 794 528 432 291 216 34
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7363
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7328
DraI TTT'AAA
Cuts at: 4667 4667
Size: 7363
DraIII CAC_nnn'GTG
Cuts at: 3427 3427
Size: 7363
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2254 6370 809
Size: 507 938 4116 1802
Fragments arranged by size:
4116 1802 938 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 2415 2559
Size: 54 1425 50 275 18 529 56 144
Cuts at: 2559 3351 3525 3630 3834 4533 4810 5699 7328
Size: 792 174 105 204 699 277 889 1629
Cuts at: 7328 8
Size: 43
Fragments arranged by size:
1629 1425 889 792 699 529 277 275
204 174 144 105 56 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4533 4810 1537
Size: 275 2721 277 4090
Fragments arranged by size:
4090 2721 277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3084 3410 4146 4455 5293 1353
Size: 1731 326 736 309 838 3423
Fragments arranged by size:
3423 1731 838 736 326 309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2322 3992 4059 5950 6324 6470
Size: 1064 138 733 1670 67 1891 374 146
Cuts at: 6470 387
Size: 1280
Fragments arranged by size:
1891 1670 1280 1064 733 374 146 138
67
EcoICRI GAG'CTC
Cuts at: 2812 3094 2812
Size: 282 7081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2544 2729 2750 2759 3131 3183
Size: 393 470 442 185 21 9 372 52
Cuts at: 3183 3239 3290 3407 3692 3821 4163 4334 4346
Size: 56 51 117 285 129 342 171 12
Cuts at: 4346 5426 6023 6810 7078 1239
Size: 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 597 470 442 393 372
342 285 268 185 171 129 117 56
52 51 21 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2029 2029
Size: 7363
EcoO109I rG'GnC_Cy
Cuts at: 2058 3130 3211 3712 3748 4479 2058
Size: 1072 81 501 36 731 4942
Fragments arranged by size:
4942 1072 731 501 81 36
EcoRI G'AATT_C
Cuts at: 4406 4672 5050 4406
Size: 266 378 6719
Fragments arranged by size:
6719 378 266
FspI TGC'GCA
Cuts at: 2243 4212 4591 5869 2243
Size: 1969 379 1278 3737
Fragments arranged by size:
3737 1969 1278 379
HaeII r_GCGC'y
Cuts at: 1678 2326 2431 2945 3485 3589 4050 6510 1678
Size: 648 105 514 540 104 461 2460 2531
Fragments arranged by size:
2531 2460 648 540 514 461 105 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2370 2891 2923 3716
Size: 32 135 32 766 32 521 32 793
Cuts at: 3716 3748 4070 4102 4376 4379 4408 4411 4598
Size: 32 322 32 274 3 29 3 187
Cuts at: 4598 4630 6004 6036 6078 6110 1373
Size: 32 1374 32 42 32 2626
Fragments arranged by size:
2626 1374 793 766 521 322 274 187
135 42 32 32 32 32 32 32
32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4707 4953 669
Size: 988 281 2769 246 3079
Fragments arranged by size:
3079 2769 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7363
HpaI GTT'AAC
Cuts at: 4953 4953
Size: 7363
KpnI G_GTAC'C
Cuts at: 4688 4688
Size: 7363
MfeI C'AATT_G
Cuts at: 4962 4962
Size: 7363
MluI A'CGCG_T
Cuts at: 7239 7239
Size: 7363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 3132 3471 3759 4266 6477 6661 1305
Size: 870 957 339 288 507 2211 184 2007
Fragments arranged by size:
2211 2007 957 870 507 339 288 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2561 3353 3632 10
Size: 54 1425 343 729 792 279 3741
Fragments arranged by size:
3741 1425 792 729 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3191 3236 3248 3620 3638 3993 5321
Size: 648 2025 45 12 372 18 355 1328
Cuts at: 5321 5680 5839 518
Size: 359 159 2042
Fragments arranged by size:
2042 2025 1328 648 372 359 355 159
45 18 12
MspA1I CmG'CkG
Cuts at: 1606 2295 2635 2737 3320 3592 3799 4779 5456
Size: 689 340 102 583 272 207 980 677
Cuts at: 5456 6604 6849 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 980 689 677 583 340 272
245 207 102
NcoI C'CATG_G
Cuts at: 513 1065 2004 2214 2275 3678 513
Size: 552 939 210 61 1403 4198
Fragments arranged by size:
4198 1403 939 552 210 61
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7363
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7363
NotI GC'GGCC_GC
Cuts at: 4810 4810
Size: 7363
NsiI A_TGCA'T
Cuts at: 3033 3033
Size: 7363
NspI r_CATG'y
Cuts at: 1132 1229 2257 2473 3262 3643 4502 4721 6266
Size: 97 1028 216 789 381 859 219 1545
Cuts at: 6266 1132
Size: 2229
Fragments arranged by size:
2229 1545 1028 859 789 381 219 216
97
PasI CC'CwG_GG
Cuts at: 1348 3437 1348
Size: 2089 5274
PciI A'CATG_T
Cuts at: 2469 3639 6262 2469
Size: 1170 2623 3570
Fragments arranged by size:
3570 2623 1170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2357 3109 3427 4257 4631 1108
Size: 1249 752 318 830 374 3840
Fragments arranged by size:
3840 1249 830 752 374 318
PfoI T'CCnGG_A
Cuts at: 2489 2877 3201 2489
Size: 388 324 6651
Fragments arranged by size:
6651 388 324
PmeI GTTT'AAAC
Cuts at: 4667 4667
Size: 7363
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5432 5464 6945 6977 5432
Size: 32 1481 32 5818
Fragments arranged by size:
5818 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3211 3712 3748 4479 3211
Size: 501 36 731 6095
Fragments arranged by size:
6095 731 501 36
PshAI GACnn'nnGTC
Cuts at: 1613 2724 1613
Size: 1111 6252
PsiI TTA'TAA
Cuts at: 4933 4933
Size: 7363
PspOMI G'GGCC_C
Cuts at: 2057 2057
Size: 7363
PstI C_TGCA'G
Cuts at: 830 3333 3549 4715 830
Size: 2503 216 1166 3478
Fragments arranged by size:
3478 2503 1166 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7363
PvuII CAG'CTG
Cuts at: 2737 3320 2737
Size: 583 6780
SacI G_AGCT'C
Cuts at: 2814 3096 2814
Size: 282 7081
SalI G'TCGA_C
Cuts at: 1936 4705 1936
Size: 2769 4594
SapI GCTCTTCn'nnn_
Cuts at: 3084 4455 3084
Size: 1371 5992
SbfI CC_TGCA'GG
Cuts at: 4715 4715
Size: 7363
ScaI AGT'ACT
Cuts at: 5611 7323 5611
Size: 1712 5651
SexAI A'CCwGG_T
Cuts at: 2847 2847
Size: 7363
SfcI C'TryA_G
Cuts at: 826 1045 3329 3545 4042 4711 5846 6527 6718
Size: 219 2284 216 497 669 1135 681 191
Cuts at: 6718 826
Size: 1471
Fragments arranged by size:
2284 1471 1135 681 669 497 219 216
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4515 4515
Size: 7363
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7363
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7363
SmaI CCC'GGG
Cuts at: 1872 2056 2063 2699 4400 4506 4515 4690 1872
Size: 184 7 636 1701 106 9 175 4545
Fragments arranged by size:
4545 1701 636 184 175 106 9 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3509 5467 6368 6630 820
Size: 197 926 1566 1958 901 262 1553
Fragments arranged by size:
1958 1566 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5154 493
Size: 4661 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7363
SphI G_CATG'C
Cuts at: 1132 4502 4721 1132
Size: 3370 219 3774
Fragments arranged by size:
3774 3370 219
SrfI GCCC'GGGC
Cuts at: 2056 2056
Size: 7363
SspI AAT'ATT
Cuts at: 5 52 5287 5
Size: 47 5235 2081
Fragments arranged by size:
5235 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7363
StyI C'CwwG_G
Cuts at: 513 1065 2004 2214 2275 3678 4802 513
Size: 552 939 210 61 1403 1124 3074
Fragments arranged by size:
3074 1403 1124 939 552 210 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 2926 3095 3353 3873 3972 4005
Size: 1255 911 435 169 258 520 99 33
Cuts at: 4005 4119 5390 5407 5560 5745 6190 325
Size: 114 1271 17 153 185 445 1498
Fragments arranged by size:
1498 1271 1255 911 520 445 435 258
185 169 153 114 99 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 3189 3489
Size: 274 80 33 51 1443 882 330 300
Cuts at: 3489 5609 7321 96
Size: 2120 1712 138
Fragments arranged by size:
2120 1712 1443 882 330 300 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 7323 7355 3896
Size: 32 3395 32 3904
Fragments arranged by size:
3904 3395 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2485 3983 4483 1316
Size: 1169 1498 500 4196
Fragments arranged by size:
4196 1498 1169 500
XbaI T'CTAG_A
Cuts at: 4699 4699
Size: 7363
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 3424 3475 3796 2875
Size: 549 51 321 6442
Fragments arranged by size:
6442 549 321 51
XhoI C'TCGA_G
Cuts at: 1943 3509 1943
Size: 1566 5797
XmaI C'CCGG_G
Cuts at: 1870 2054 2061 2697 4398 4504 4513 4688 1870
Size: 184 7 636 1701 106 9 175 4545
Fragments arranged by size:
4545 1701 636 184 175 106 9 7
XmnI GAAnn'nnTTC
Cuts at: 3224 5492 3224
Size: 2268 5095
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5171 276
Size: 53 83 186 936 3637 2468
Fragments arranged by size:
3637 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SanDI SfoI SwaI