(Linear) (Six-base) MAPSORT of: KIEE3434.seq Check: 3587 from: 1 to: 2658 KIEE3434.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1183 2658 Size: 1183 1475 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 539 1234 1687 2658 Size: 97 442 695 453 971 Fragments arranged by size: 971 695 453 442 97 AflIII A'CryG_T Cuts at: 0 464 676 1634 2658 Size: 464 212 958 1024 Fragments arranged by size: 1024 958 464 212 AleI CACnn'nnGTG Cuts at: 0 1243 2658 Size: 1243 1415 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2576 2658 Size: 2576 82 AlwNI CAG_nnn'CTG Cuts at: 0 96 552 2582 2626 2658 Size: 96 456 2030 44 32 Fragments arranged by size: 2030 456 96 44 32 ApaI G_GGCC'C Cuts at: 0 56 2658 Size: 56 2602 ApaLI G'TGCA_C Cuts at: 0 1759 1948 2181 2363 2658 Size: 1759 189 233 182 295 Fragments arranged by size: 1759 295 233 189 182 ApoI r'AATT_y Cuts at: 0 1219 2401 2658 Size: 1219 1182 257 Fragments arranged by size: 1219 1182 257 AvaI C'yCGr_G Cuts at: 0 49 56 692 1504 2071 2393 2499 2508 Size: 49 7 636 812 567 322 106 9 Cuts at: 2508 2658 Size: 150 Fragments arranged by size: 812 636 567 322 150 106 49 9 7 BanI G'GyrC_C Cuts at: 0 280 2658 Size: 280 2378 BanII G_rGCy'C Cuts at: 0 56 385 809 1091 2059 2658 Size: 56 329 424 282 968 599 Fragments arranged by size: 968 599 424 329 282 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 527 1078 1675 2658 Size: 527 551 597 983 Fragments arranged by size: 983 597 551 527 BbvCI CC'TCA_GC Cuts at: 0 1573 1774 2658 Size: 1573 201 884 Fragments arranged by size: 1573 884 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 303 337 733 767 2658 Size: 303 34 396 34 1891 Fragments arranged by size: 1891 396 303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 136 2658 Size: 136 2522 BfrBI ATG'CAT Cuts at: 0 1026 2658 Size: 1026 1632 BglI GCCn_nnn'nGGC Cuts at: 0 186 409 2194 2257 2510 2569 2658 Size: 186 223 1785 63 253 59 89 Fragments arranged by size: 1785 253 223 186 89 63 59 BglII A'GATC_T Cuts at: 0 2000 2658 Size: 2000 658 Bme1580I G_kGCm'C Cuts at: 0 56 605 1763 1952 2185 2367 2658 Size: 56 549 1158 189 233 182 291 Fragments arranged by size: 1158 549 291 233 189 182 56 BmgBI CAC'GTC Cuts at: 0 1287 1320 2658 Size: 1287 33 1338 Fragments arranged by size: 1338 1287 33 BmrI ACTGGGnnnn_n' Cuts at: 0 605 2658 Size: 605 2053 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 53 789 2078 2658 Size: 53 736 1289 580 Fragments arranged by size: 1289 736 580 53 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 745 754 1126 1178 1285 1402 1816 Size: 724 21 9 372 52 107 117 414 Cuts at: 1816 2158 2329 2341 2658 Size: 342 171 12 317 Fragments arranged by size: 724 414 372 342 317 171 117 107 52 21 12 9 Bpu10I CC'TnA_GC Cuts at: 0 545 1573 1701 1774 2658 Size: 545 1028 128 73 884 Fragments arranged by size: 1028 884 545 128 73 BsaI GGTCTCn'nnnn_ Cuts at: 0 730 852 1443 1675 1957 2642 2658 Size: 730 122 591 232 282 685 16 Fragments arranged by size: 730 685 591 282 232 122 16 BsaAI yAC'GTr Cuts at: 0 1338 2658 Size: 1338 1320 BsaBI GATnn'nnATC Cuts at: 0 1424 1609 2658 Size: 1424 185 1049 Fragments arranged by size: 1424 1049 185 BsaWI w'CCGG_w Cuts at: 0 563 835 1748 2376 2658 Size: 563 272 913 628 282 Fragments arranged by size: 913 628 563 282 272 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 333 363 558 588 1891 1921 2314 2344 Size: 333 30 195 30 1303 30 393 30 Cuts at: 2344 2658 Size: 314 Fragments arranged by size: 1303 393 333 314 195 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 721 2089 2092 2155 2164 2658 Size: 721 1368 3 63 9 494 Fragments arranged by size: 1368 721 494 63 9 3 BseYI C'CCAG_C Cuts at: 0 630 956 2619 2658 Size: 630 326 1663 39 Fragments arranged by size: 1663 630 326 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 1291 1340 2658 Size: 382 909 49 1318 Fragments arranged by size: 1318 909 382 49 BsiEI CG_ry'CG Cuts at: 0 14 225 1734 2531 2658 Size: 14 211 1509 797 127 Fragments arranged by size: 1509 797 211 127 14 BsiHKAI G_wGCw'C Cuts at: 0 809 1091 1505 1763 1952 2185 2367 2555 Size: 809 282 414 258 189 233 182 188 Cuts at: 2555 2658 Size: 103 Fragments arranged by size: 809 414 282 258 233 189 188 182 103 Bsp1286I G_dGCh'C Cuts at: 0 56 385 605 809 1091 1505 1763 1952 Size: 56 329 220 204 282 414 258 189 Cuts at: 1952 2059 2185 2367 2555 2658 Size: 107 126 182 188 103 Fragments arranged by size: 414 329 282 258 220 204 189 188 182 126 107 103 56 BspEI T'CCGG_A Cuts at: 0 563 835 1748 2376 2658 Size: 563 272 913 628 282 Fragments arranged by size: 913 628 563 282 272 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 821 1183 1217 2658 Size: 821 362 34 1441 Fragments arranged by size: 1441 821 362 34 BsrBI CCG'CTC Cuts at: 0 168 174 446 1166 1203 2658 Size: 168 6 272 720 37 1455 Fragments arranged by size: 1455 720 272 168 37 6 BsrFI r'CCGG_y Cuts at: 0 185 224 283 2658 Size: 185 39 59 2375 Fragments arranged by size: 2375 185 59 39 BsrGI T'GTAC_A Cuts at: 0 1184 1484 2658 Size: 1184 300 1174 Fragments arranged by size: 1184 1174 300 BssHII G'CGCG_C Cuts at: 0 159 198 2658 Size: 159 39 2460 Fragments arranged by size: 2460 159 39 BssSI C'ACGA_G Cuts at: 0 391 2658 Size: 391 2267 BstAPI GCAn_nnn'nTGC Cuts at: 0 1363 2203 2582 2658 Size: 1363 840 379 76 Fragments arranged by size: 1363 840 379 76 BstEII G'GTnAC_C Cuts at: 0 498 1849 2658 Size: 498 1351 809 Fragments arranged by size: 1351 809 498 BstXI CCAn_nnnn'nTGG Cuts at: 0 277 1794 2658 Size: 277 1517 864 Fragments arranged by size: 1517 864 277 BstYI r'GATC_y Cuts at: 0 896 2000 2658 Size: 896 1104 658 Fragments arranged by size: 1104 896 658 BtgI C'CryG_G Cuts at: 0 209 270 1523 1628 1673 1910 2522 2658 Size: 209 61 1253 105 45 237 612 136 Fragments arranged by size: 1253 612 237 209 136 105 61 45 BtsI GCAGTG_nn' Cuts at: 0 43 1373 1901 2333 2367 2583 2658 Size: 43 1330 528 432 34 216 75 Fragments arranged by size: 1330 528 432 216 75 43 34 DraIII CAC_nnn'GTG Cuts at: 0 1422 2658 Size: 1422 1236 DrdI GACnn_nn'nnGTC Cuts at: 0 249 2658 Size: 249 2409 EaeI y'GGCC_r Cuts at: 0 354 410 554 1346 1520 1625 1829 2528 Size: 354 56 144 792 174 105 204 699 Cuts at: 2528 2658 Size: 130 Fragments arranged by size: 792 699 354 204 174 144 130 105 56 EagI C'GGCC_G Cuts at: 0 2528 2658 Size: 2528 130 EarI CTCTTCn'nnn_ Cuts at: 0 1079 1405 2141 2450 2658 Size: 1079 326 736 309 208 Fragments arranged by size: 1079 736 326 309 208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 317 1987 2054 2658 Size: 317 1670 67 604 Fragments arranged by size: 1670 604 317 67 EcoICRI GAG'CTC Cuts at: 0 807 1089 2658 Size: 807 282 1569 Fragments arranged by size: 1569 807 282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 539 724 745 754 1126 1178 1234 Size: 97 442 185 21 9 372 52 56 Cuts at: 1234 1285 1402 1687 1816 2158 2329 2341 2658 Size: 51 117 285 129 342 171 12 317 Fragments arranged by size: 442 372 342 317 285 185 171 129 117 97 56 52 51 21 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 24 2658 Size: 24 2634 EcoO109I rG'GnC_Cy Cuts at: 0 53 1125 1206 1707 1743 2474 2658 Size: 53 1072 81 501 36 731 184 Fragments arranged by size: 1072 731 501 184 81 53 36 EcoRI G'AATT_C Cuts at: 0 2401 2658 Size: 2401 257 FspI TGC'GCA Cuts at: 0 238 2207 2586 2658 Size: 238 1969 379 72 Fragments arranged by size: 1969 379 238 72 HaeII r_GCGC'y Cuts at: 0 321 426 940 1480 1584 2045 2658 Size: 321 105 514 540 104 461 613 Fragments arranged by size: 613 540 514 461 321 105 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 333 365 886 918 1711 1743 2065 2097 Size: 333 32 521 32 793 32 322 32 Cuts at: 2097 2371 2374 2403 2406 2593 2625 2658 Size: 274 3 29 3 187 32 33 Fragments arranged by size: 793 521 333 322 274 187 33 32 32 32 32 32 29 3 3 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 170 1127 1466 1754 2261 2658 Size: 170 957 339 288 507 397 Fragments arranged by size: 957 507 397 339 288 170 MscI TGG'CCA Cuts at: 0 556 1348 1627 2658 Size: 556 792 279 1031 Fragments arranged by size: 1031 792 556 279 MslI CAynn'nnrTG Cuts at: 0 1186 1231 1243 1615 1633 1988 2658 Size: 1186 45 12 372 18 355 670 Fragments arranged by size: 1186 670 372 355 45 18 12 MspA1I CmG'CkG Cuts at: 0 290 630 732 1315 1587 1794 2658 Size: 290 340 102 583 272 207 864 Fragments arranged by size: 864 583 340 290 272 207 102 NcoI C'CATG_G Cuts at: 0 209 270 1673 2658 Size: 209 61 1403 985 Fragments arranged by size: 1403 985 209 61 NsiI A_TGCA'T Cuts at: 0 1028 2658 Size: 1028 1630 NspI r_CATG'y Cuts at: 0 252 468 1257 1638 2497 2658 Size: 252 216 789 381 859 161 Fragments arranged by size: 859 789 381 252 216 161 PasI CC'CwG_GG Cuts at: 0 1432 2658 Size: 1432 1226 PciI A'CATG_T Cuts at: 0 464 1634 2658 Size: 464 1170 1024 Fragments arranged by size: 1170 1024 464 PflMI CCAn_nnn'nTGG Cuts at: 0 352 1104 1422 2252 2626 2658 Size: 352 752 318 830 374 32 Fragments arranged by size: 830 752 374 352 318 32 PfoI T'CCnGG_A Cuts at: 0 484 872 1196 2658 Size: 484 388 324 1462 Fragments arranged by size: 1462 484 388 324 PpuMI rG'GwC_Cy Cuts at: 0 1206 1707 1743 2474 2658 Size: 1206 501 36 731 184 Fragments arranged by size: 1206 731 501 184 36 PshAI GACnn'nnGTC Cuts at: 0 719 2658 Size: 719 1939 PspOMI G'GGCC_C Cuts at: 0 52 2658 Size: 52 2606 PstI C_TGCA'G Cuts at: 0 1328 1544 2658 Size: 1328 216 1114 Fragments arranged by size: 1328 1114 216 PvuII CAG'CTG Cuts at: 0 732 1315 2658 Size: 732 583 1343 Fragments arranged by size: 1343 732 583 SacI G_AGCT'C Cuts at: 0 809 1091 2658 Size: 809 282 1567 Fragments arranged by size: 1567 809 282 SapI GCTCTTCn'nnn_ Cuts at: 0 1079 2450 2658 Size: 1079 1371 208 Fragments arranged by size: 1371 1079 208 SexAI A'CCwGG_T Cuts at: 0 842 2658 Size: 842 1816 SfcI C'TryA_G Cuts at: 0 1324 1540 2037 2658 Size: 1324 216 497 621 Fragments arranged by size: 1324 621 497 216 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2510 2658 Size: 2510 148 SmaI CCC'GGG Cuts at: 0 51 58 694 2395 2501 2510 2658 Size: 51 7 636 1701 106 9 148 Fragments arranged by size: 1701 636 148 106 51 9 7 SmlI C'TyrA_G Cuts at: 0 1504 2658 Size: 1504 1154 SphI G_CATG'C Cuts at: 0 2497 2658 Size: 2497 161 SrfI GCCC'GGGC Cuts at: 0 51 2658 Size: 51 2607 StyI C'CwwG_G Cuts at: 0 209 270 1673 2658 Size: 209 61 1403 985 Fragments arranged by size: 1403 985 209 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 486 921 1090 1348 1868 1967 2000 2114 Size: 486 435 169 258 520 99 33 114 Cuts at: 2114 2658 Size: 544 Fragments arranged by size: 544 520 486 435 258 169 114 99 33 TatI w'GTAC_w Cuts at: 0 854 1184 1484 2658 Size: 854 330 300 1174 Fragments arranged by size: 1174 854 330 300 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1891 1923 2658 Size: 1891 32 735 Fragments arranged by size: 1891 735 32 Tth111I GACn'n_nGTC Cuts at: 0 480 1978 2478 2658 Size: 480 1498 500 180 Fragments arranged by size: 1498 500 480 180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 870 1419 1470 1791 2658 Size: 870 549 51 321 867 Fragments arranged by size: 870 867 549 321 51 XhoI C'TCGA_G Cuts at: 0 1504 2658 Size: 1504 1154 XmaI C'CCGG_G Cuts at: 0 49 56 692 2393 2499 2508 2658 Size: 49 7 636 1701 106 9 150 Fragments arranged by size: 1701 636 150 106 49 9 7 XmnI GAAnn'nnTTC Cuts at: 0 1219 2658 Size: 1219 1439 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SapI SexAI SfcI SfiI SmaI SmlI SphI SrfI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BclI BlpI BmtI BpuEI BsaHI BsiWI BsmI BsmBI BspHI BsrDI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SspI StuI SwaI XbaI ZraI