Restriction map for
pFN21AE3604
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 143 GGTACC G^GTACC
AccI 730 GTCGAC GT^[AC][GT]AC
AflIII 184 ACACGT A^C[GA][CT]GT
AlwNI 573 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 143 GGTACC G^GTACC
BalI 387 TGGCCA TGG^CCA
BanI 143 GGTACC G^G[CT][GA]CC
BbsI 742 GAAGAC GAAGAC(2/6)
BpuAI 742 GAAGAC GAAGAC(2/6)
BseRI 928 CTCCTC GAGGAG(10/8)
BsgI 579 CTGCAC GTGCAG(16/14)
BsiEI 728 CGGTCG CG[GA][CT]^CG
BsmBI 878 CGTCTC CGTCTC(1/5)
Bsp1407I 378 TGTACA T^GTACA
BsrGI 378 TGTACA T^GTACA
BstYI 765 GGATCT [GA]^GATC[CT]
CfoI 158 GCGC GCG^C
DraII 914 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 387 TGGCCA [CT]^GGCC[GA]
EarI 833 CTCTTC CTCTTC(1/4)
EcoNI 927 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 914 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 343 GATATC GAT^ATC
HaeII 157 GGCGCT [GA]GCGC^[CT]
HhaI 158 GCGC GCG^C
HinP1I 158 GCGC G^CGC
HincII 730 GTCGAC GT[CT]^[GA]AC
HindII 730 GTCGAC GT[CT]^[GA]AC
HinfI 434 GAATC G^A[ACGT]TC
HphI 517 GGTGA GGTGA(8/7)
KpnI 143 GGTACC GGTAC^C
Ksp632I 833 CTCTTC CTCTTC(1/4)
MflI 765 GGATCT [GA]^GATC[CT]
MluNI 387 TGGCCA TGG^CCA
MscI 387 TGGCCA TGG^CCA
MseI 370 TTAA T^TAA
MslI 593 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 814 CAGCTG C[AC]G^C[GT]G
NspI 1024 GCATGC [GA]CATG^[CT]
PflMI 756 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 914 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 814 CAGCTG CAG^CTG
SalI 730 GTCGAC G^TCGAC
SfaNI 792 GATGC GCATC(5/9)
SphI 1024 GCATGC GCATG^C
SspBI 378 TGTACA T^GTACA
TaqI 731 TCGA T^CGA
TfiI 434 GAATC G^A[AT]TC
Tru9I 370 TTAA T^TAA
TthHB8I 731 TCGA T^CGA
Van91I 756 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 64 CCATGTTGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 765 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AlwI 124 GATCC GGATC(4/5)
765 GGATC
BbvI 1053 GCAGC GCAGC(8/12)
816 GCTGC
BglI 130 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
149 GCCGAGAGGGC
BmyI 180 GGGCAC G[AGT]GC[ACT]^C
999 GTGCCC
BsmAI 575 GTCTC GTCTC(1/5)
879 GTCTC
BsmFI 724 GTCCC GGGAC(10/14)
975 GTCCC
Bsp1286I 180 GGGCAC G[AGT]GC[ACT]^C
999 GTGCCC
FauI 1004 CCCGC CCCGC(4/6)
1049 GCGGG
PstI 442 CTGCAG CTGCA^G
595 CTGCAG
SfcI 442 CTGCAG C^T[GA][CT]AG
595 CTGCAG
TseI 816 GCTGC G^C[AT]GC
1053 GCAGC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BcnI BpmI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI Tsp45I Tsp509I TspRI