Restriction map for
pFN21AE3172
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI Sau96I SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 116 ACATGT A^C[GA][CT]GT
Asp700I 1000 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 346 GAGCTC G[AT]GC[AT]^C
BanII 346 GAGCTC G[GA]GC[CT]^C
BbsI 120 GTCTTC GAAGAC(2/6)
BpmI 1153 CTGGAG CTGGAG(16/14)
BpuAI 120 GTCTTC GAAGAC(2/6)
BsaAI 1070 TACGTA [CT]AC^GT[GA]
BsaBI 259 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 346 GAGCTC G[AT]GC[AT]^C
BsmAI 828 GTCTC GTCTC(1/5)
BsmFI 797 GGGAC GGGAC(10/14)
BspLU11I 116 ACATGT A^CATGT
BsrDI 568 GCAATG GCAATG(2/0)
BsrI 1144 ACTGG ACTGG(1/-1)
BstXI 53 CCAGGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 264 AGATCC [GA]^GATC[CT]
DraI 396 TTTAAA TTT^AAA
EcoNI 966 CCTAGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 971 CCAAGG C^C[AT][AT]GG
HaeIII 591 GGCC GG^CC
HinfI 877 GATTC G^A[ACGT]TC
MaeII 1071 ACGT A^CGT
MamI 259 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 264 AGATCC [GA]^GATC[CT]
MspA1I 134 CAGCTG C[AC]G^C[GT]G
NspI 116 ACATGT [GA]CATG^[CT]
PstI 872 CTGCAG CTGCA^G
PvuII 134 CAGCTG CAG^CTG
SacI 346 GAGCTC GAGCT^C
SexAI 52 ACCAGGT A^CC[AT]GGT
SfcI 872 CTGCAG C^T[GA][CT]AG
SnaBI 1070 TACGTA TAC^GTA
StyI 971 CCAAGG C^C[AT][AT]GG
TfiI 877 GATTC G^A[AT]TC
XhoII 264 AGATCC [GA]^GATC[CT]
XmnI 1000 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 161 GTAC GT^AC
322 GTAC
AlwI 265 GATCC GGATC(4/5)
715 GATCC
BanI 63 GGTGCC G^G[CT][GA]CC
583 GGTGCC
BmyI 346 GAGCTC G[AGT]GC[ACT]^C
584 GTGCCC
BsaJI 587 CCCAGG C^C[ACGT][ACGT]GG
971 CCAAGG
BsiYI 74 CCCTTCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
966 CCTAGCCAAGG
BslI 74 CCCTTCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
966 CCTAGCCAAGG
BsmI 1000 GAATGC GAATGC(1/-1)
1167 GCATTC
Bsp1286I 346 GAGCTC G[AGT]GC[ACT]^C
584 GTGCCC
HphI 50 TCACC GGTGA(8/7)
1052 TCACC
MaeIII 139 GTTAC ^GT[ACGT]AC
1040 GTAAC
MslI 291 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
564 CATAGCAATG
NlaIV 63 GGTGCC GG[ACGT]^[ACGT]CC
583 GGTGCC
RsaI 161 GTAC GT^AC
322 GTAC
SapI 175 GAAGAGC GCTCTTC(1/4)
343 GAAGAGC
SmlI 246 CTCAAG C^T[CT][GA]AG
1172 CTCAAG
TspRI 336 CACTG CA[GC]TG[ACGT][ACGT]^
504 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BfaI BssKI BstF5I
BstNI DdeI DpnII EarI EcoRII
Fnu4HI FokI ItaI Ksp632I MaeI
MboI MboII MnlI MseI MvaI
NdeII NlaIII Sau3AI ScrFI SspI
Tru9I TseI Tsp509I