(Circular) (Six-base) MAPSORT of: pFN21AE3172.seq Check: 568 from: 1 to: 5914 pFN21AE3172 5914 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3257 1937 Size: 1320 4594 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2029 2199 2364 3977 5361 5629 1632 Size: 397 170 165 1613 1384 268 1917 Fragments arranged by size: 1917 1613 1384 397 268 170 165 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 2121 4813 5790 1203 Size: 918 2692 977 1327 Fragments arranged by size: 2692 1327 977 918 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3022 1184 Size: 1838 4076 AlwNI CAG_nnn'CTG Cuts at: 3657 5229 3657 Size: 1572 4342 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 3223 3449 3601 5710 1493 Size: 1730 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 3239 1870 Size: 73 1296 4545 Fragments arranged by size: 4545 1296 73 BamHI G'GATC_C Cuts at: 3244 3611 3244 Size: 367 5547 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2588 3235 4690 618 Size: 325 217 627 281 520 647 1455 1842 Fragments arranged by size: 1842 1455 647 627 520 325 281 217 BanII G_rGCy'C Cuts at: 1391 2355 3233 1391 Size: 964 878 4072 Fragments arranged by size: 4072 964 878 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 928 Size: 971 219 4724 Fragments arranged by size: 4724 971 219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4105 4139 1903 Size: 34 2168 34 3678 Fragments arranged by size: 3678 2168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 2593 3975 5131 1790 Size: 803 1382 1156 2573 Fragments arranged by size: 2573 1382 1156 803 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3645 4603 442 Size: 416 53 83 399 24 2228 958 1753 Fragments arranged by size: 2228 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3179 4574 1239 Size: 1940 1395 2579 Fragments arranged by size: 2579 1940 1395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2236 3162 4039 4904 5202 2236 Size: 926 877 865 298 2948 Fragments arranged by size: 2948 926 877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4577 882 Size: 617 3078 2219 Fragments arranged by size: 3078 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3077 3705 493 Size: 711 1873 628 2702 Fragments arranged by size: 2702 1873 711 628 BsaBI GATnn'nnATC Cuts at: 2268 5908 2268 Size: 3640 2274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3721 4103 4738 275 Size: 53 83 186 936 2188 382 635 1451 Fragments arranged by size: 2188 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5689 Fragments arranged by size: 5689 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5914 BseYI C'CCAG_C Cuts at: 2679 5117 5613 2679 Size: 2438 496 2980 Fragments arranged by size: 2980 2438 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2355 3975 4060 5131 2355 Size: 1620 85 1071 3138 Fragments arranged by size: 3138 1620 1071 85 BsmI GAATG_Cn' Cuts at: 3011 3172 3425 3518 3011 Size: 161 253 93 5407 Fragments arranged by size: 5407 253 161 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2355 2593 3233 3975 4060 5131 1391 Size: 399 565 238 640 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 565 399 238 85 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 844 Size: 2425 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3801 1394 Size: 429 1978 3507 Fragments arranged by size: 3507 1978 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2580 4409 4583 65 Size: 1183 1332 1829 174 1396 Fragments arranged by size: 1829 1396 1332 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4558 4745 1700 Size: 110 2748 187 2869 Fragments arranged by size: 2869 2748 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2065 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2269 3244 3611 3996 4013 5465 5551 5563 Size: 297 975 367 385 17 1452 86 12 Cuts at: 5563 5909 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 975 385 367 346 297 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 3430 4224 4244 3430 Size: 794 20 5100 Fragments arranged by size: 5100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 2403 3218 2403 Size: 815 5099 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3361 4250 5879 Size: 54 1425 50 275 18 1531 889 1629 Cuts at: 5879 8 Size: 43 Fragments arranged by size: 1629 1531 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2175 2343 3844 1353 Size: 731 91 168 1501 3423 Fragments arranged by size: 3423 1501 731 168 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2353 2353 Size: 5914 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2199 2364 3179 3977 4574 5361 Size: 393 397 170 165 815 798 597 787 Cuts at: 5361 5629 1239 Size: 268 1524 Fragments arranged by size: 1524 815 798 787 597 397 393 268 170 165 EcoNI CCTnn'n_nnAGG Cuts at: 2975 2975 Size: 5914 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 5061 1678 Size: 3383 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4555 4587 4629 4661 1373 Size: 32 135 32 2983 32 42 32 2626 Fragments arranged by size: 2983 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2172 5028 5212 1305 Size: 867 2856 184 2007 Fragments arranged by size: 2856 2007 867 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4092 Fragments arranged by size: 4092 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 2573 3872 4231 4390 518 Size: 648 1134 273 1299 359 159 2042 Fragments arranged by size: 2042 1299 1134 648 359 273 159 MspA1I CmG'CkG Cuts at: 1606 2141 3330 4007 5155 5400 1606 Size: 535 1189 677 1148 245 2120 Fragments arranged by size: 2120 1189 1148 677 535 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4423 Fragments arranged by size: 4423 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2125 3272 4817 1132 Size: 97 896 1147 1545 2229 Fragments arranged by size: 2229 1545 1147 896 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 2121 4813 2121 Size: 2692 3222 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PstI C_TGCA'G Cuts at: 830 2881 3266 830 Size: 2051 385 3478 Fragments arranged by size: 3478 2051 385 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2141 2141 Size: 5914 SacI G_AGCT'C Cuts at: 2355 2355 Size: 5914 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SapI GCTCTTCn'nnn_ Cuts at: 2175 2343 2175 Size: 168 5746 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SexAI A'CCwGG_T Cuts at: 2057 2057 Size: 5914 SfcI C'TryA_G Cuts at: 826 1045 2877 3262 4397 5078 5269 826 Size: 219 1832 385 1135 681 191 1471 Fragments arranged by size: 1832 1471 1135 681 385 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2251 3177 4018 4919 5181 820 Size: 197 926 308 926 841 901 262 1553 Fragments arranged by size: 1553 926 926 901 841 308 262 197 SnaBI TAC'GTA Cuts at: 493 3077 3705 493 Size: 2584 628 2702 Fragments arranged by size: 2702 2584 628 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SspI AAT'ATT Cuts at: 5 52 2399 2408 3026 3838 5 Size: 47 2347 9 618 812 2081 Fragments arranged by size: 2347 2081 812 618 47 9 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2004 2976 3353 513 Size: 552 939 972 377 3074 Fragments arranged by size: 3074 972 939 552 377 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3941 3958 4111 4296 4741 325 Size: 1255 2361 17 153 185 445 1498 Fragments arranged by size: 2361 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2165 4160 5872 Size: 274 80 33 51 1443 188 1995 1712 Cuts at: 5872 96 Size: 138 Fragments arranged by size: 1995 1712 1443 274 188 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5874 5906 5874 Size: 32 5882 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5914 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 3009 4043 3009 Size: 1034 4880 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI