Restriction map for
pFN21AE3164
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvaI AvaII AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeII MfeI
MluI MroI MunI NciI NdeI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 991 GTAGAC GT^[AC][GT]AC
AcsI 578 AAATTC [GA]^AATT[CT]
AcyI 913 GGCGCC G[GA]^CG[CT]C
AlwNI 1033 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 635 GGGCCC GGGCC^C
ApoI 578 AAATTC [GA]^AATT[CT]
AspI 656 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 913 GGCGCC G^G[CT][GA]CC
BanII 635 GGGCCC G[GA]GC[CT]^C
BbeI 913 GGCGCC GGCGC^C
BfaI 439 CTAG C^TAG
BglII 923 AGATCT A^GATCT
BpmI 769 CTGGAG CTGGAG(16/14)
BsaBI 487 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 913 GGCGCC G[GA]^CG[CT]C
BsaI 211 GAGACC GGTCTC(1/5)
BseRI 979 GAGGAG GAGGAG(10/8)
BsgI 141 CTGCAC GTGCAG(16/14)
BsiEI 134 CGGCCG CG[GA][CT]^CG
BspHI 1023 TCATGA T^CATGA
BspMI 460 GCAGGT ACCTGC(4/8)
BsrBI 95 CCGCTC CCGCTC(-3/-3)
BsrDI 1041 GCAATG GCAATG(2/0)
BssHII 60 GCGCGC G^CGCGC
EagI 134 CGGCCG C^GGCCG
EarI 411 GAAGAG CTCTTC(1/4)
EclXI 134 CGGCCG C^GGCCG
Eco52I 134 CGGCCG C^GGCCG
EcoRV 1066 GATATC GAT^ATC
Hin1I 913 GGCGCC G[GA]^CG[CT]C
KasI 913 GGCGCC G^GCGCC
Ksp632I 411 GAAGAG CTCTTC(1/4)
KspI 890 CCGCGG CCGC^GG
MaeI 439 CTAG C^TAG
MamI 487 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 466 TTAA T^TAA
MslI 1140 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 81 GCCGGC GCC^GGC
NarI 913 GGCGCC GG^CGCC
NcoI 1118 CCATGG C^CATGG
NgoMIV 81 GCCGGC G^CCGGC
NheI 438 GCTAGC G^CTAGC
PflFI 656 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 715 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 956 CTGCAG CTGCA^G
PvuII 302 CAGCTG CAG^CTG
RcaI 1023 TCATGA T^CATGA
SacII 890 CCGCGG CCGC^GG
SfoI 913 GGCGCC GGC^GCC
StuI 765 AGGCCT AGG^CCT
Tru9I 466 TTAA T^TAA
Tth111I 656 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 696 GTGCAC G^TGCAC
757 GTGCAC
AlwI 897 GATCC GGATC(4/5)
486 GGATC
ApaLI 696 GTGCAC G^TGCAC
757 GTGCAC
AspHI 696 GTGCAC G[AT]GC[AT]^C
757 GTGCAC
BbsI 339 GAAGAC GAAGAC(2/6)
115 GTCTTC
BpuAI 339 GAAGAC GAAGAC(2/6)
115 GTCTTC
BsiHKAI 696 GTGCAC G[AT]GC[AT]^C
757 GTGCAC
BsrFI 81 GCCGGC [GA]^CCGG[CT]
103 ACCGGC
BsrI 705 ACTGG ACTGG(1/-1)
639 CCAGT
Cfr10I 81 GCCGGC [GA]^CCGG[CT]
103 ACCGGC
DraII 634 AGGGCCC [GA]G^G[ACGT]CC[CT]
962 AGGCCCC
EcoNI 347 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
972 CCTGATTGAGG
EcoO109I 634 AGGGCCC [GA]G^G[ACGT]CC[CT]
962 AGGCCCC
EcoT14I 174 CCTTGG C^C[AT][AT]GG
1118 CCATGG
HaeII 170 AGCGCC [GA]GCGC^[CT]
913 GGCGCC
HapII 82 CCGG C^CGG
104 CCGG
HpaII 82 CCGG C^CGG
104 CCGG
HphI 1005 GGTGA GGTGA(8/7)
547 TCACC
MaeIII 725 GTCAC ^GT[ACGT]AC
1006 GTGAC
MspA1I 302 CAGCTG C[AC]G^C[GT]G
890 CCGCGG
MspI 82 CCGG C^CGG
104 CCGG
StyI 174 CCTTGG C^C[AT][AT]GG
1118 CCATGG
Tsp45I 725 GTCAC ^GT[GC]AC
1006 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI Asp700I BalI
BbvI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HinfI ItaI
MboI MboII MflI MluNI MnlI
MscI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp509I TspRI XhoII XmnI