(Circular) (Six-base) MAPSORT of: pFN21AE3164.seq Check: 7954 from: 1 to: 5860 pFN21AE3164 5860 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2997 3203 1937 Size: 1060 206 4594 Fragments arranged by size: 4594 1060 206 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2549 3111 3923 5307 5575 1632 Size: 731 186 562 812 1384 268 1917 Fragments arranged by size: 1917 1384 812 731 562 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2407 2439 2407 Size: 32 5828 AlwNI CAG_nnn'CTG Cuts at: 3043 3603 5175 3043 Size: 560 1572 3728 Fragments arranged by size: 3728 1572 560 ApaI G_GGCC'C Cuts at: 2644 2644 Size: 5860 ApaLI G'TGCA_C Cuts at: 2701 2762 3917 5073 2701 Size: 61 1155 1156 3488 Fragments arranged by size: 3488 1156 1155 61 ApoI r'AATT_y Cuts at: 1493 2583 3169 3395 3547 5656 1493 Size: 1090 586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1090 586 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 3185 1870 Size: 73 1242 4545 Fragments arranged by size: 4545 1242 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2918 3181 4636 618 Size: 325 217 627 1131 263 1455 1842 Fragments arranged by size: 1842 1455 1131 627 325 263 217 BanII G_rGCy'C Cuts at: 1391 2644 3179 1391 Size: 1253 535 4072 Fragments arranged by size: 4072 1253 535 BbeI G_GCGC'C Cuts at: 2922 2922 Size: 5860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2113 2351 928 Size: 971 214 238 4437 Fragments arranged by size: 4437 971 238 214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4051 4085 1903 Size: 34 50 34 2030 34 3678 Fragments arranged by size: 3678 2030 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2928 5855 2928 Size: 2927 2933 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2644 2705 2766 3921 5077 1790 Size: 854 61 61 1155 1156 2573 Fragments arranged by size: 2573 1156 1155 854 61 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2638 3591 4549 Size: 416 53 83 399 24 1221 953 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1221 958 953 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2447 1056 Size: 1391 4469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2795 4520 1239 Size: 1556 1725 2579 Fragments arranged by size: 2579 1725 1556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 4523 882 Size: 617 711 2313 2219 Fragments arranged by size: 2313 2219 711 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 2496 5854 2496 Size: 3358 2502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2919 3667 4049 4684 Size: 53 83 186 936 1386 748 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1451 1386 936 748 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2999 1186 Size: 1813 4047 BseYI C'CCAG_C Cuts at: 5063 5559 5063 Size: 496 5364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2131 1163 Size: 573 395 4892 Fragments arranged by size: 4892 573 395 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 3310 4071 5099 1540 Size: 275 186 141 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 141 BsiHKAI G_wGCw'C Cuts at: 2705 2766 3921 4006 5077 2705 Size: 61 1155 85 1071 3488 Fragments arranged by size: 3488 1155 1071 85 61 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2644 2705 2766 3179 3921 4006 5077 Size: 399 854 61 61 413 742 85 1071 Cuts at: 5077 1391 Size: 2174 Fragments arranged by size: 2174 1071 854 742 413 399 85 61 61 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 3028 3749 5479 3028 Size: 721 1730 3409 Fragments arranged by size: 3409 1730 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2456 3215 844 Size: 1612 759 3489 Fragments arranged by size: 3489 1612 759 BsrBI CCG'CTC Cuts at: 1394 1823 2102 3747 1394 Size: 429 279 1645 3507 Fragments arranged by size: 3507 1645 429 279 BsrDI GCAATG_nn' Cuts at: 65 1248 3053 4355 4529 65 Size: 1183 1805 1302 174 1396 Fragments arranged by size: 1805 1396 1302 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2086 2108 4504 4691 1700 Size: 110 276 22 2396 187 2869 Fragments arranged by size: 2869 2396 276 187 110 22 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 2065 1925 Size: 140 5720 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2491 2901 2928 3190 3557 3942 3959 5411 Size: 519 410 27 262 367 385 17 1452 Cuts at: 5411 5497 5509 5855 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 519 410 385 367 346 262 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2136 2895 2947 3123 513 Size: 552 1071 759 52 176 3250 Fragments arranged by size: 3250 1071 759 552 176 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2827 487 Size: 683 56 1601 3520 Fragments arranged by size: 3520 1601 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4170 4190 3376 Size: 794 20 5046 Fragments arranged by size: 5046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2835 2870 529 Size: 35 2271 35 3519 Fragments arranged by size: 3519 2271 35 35 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2127 2139 Size: 54 1425 50 275 18 258 39 12 Cuts at: 2139 2157 2260 2676 2793 2940 3307 4196 5825 Size: 18 103 416 117 147 367 889 1629 Cuts at: 5825 8 Size: 43 Fragments arranged by size: 1629 1425 889 416 367 275 258 147 117 103 54 50 43 39 18 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2139 3307 1537 Size: 275 327 1168 4090 Fragments arranged by size: 4090 1168 327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 3790 1353 Size: 1058 1379 3423 Fragments arranged by size: 3423 1379 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2549 2795 3111 3923 4520 5307 Size: 393 731 186 246 316 812 597 787 Cuts at: 5307 5575 1239 Size: 268 1524 Fragments arranged by size: 1524 812 787 731 597 393 316 268 246 186 EcoNI CCTnn'n_nnAGG Cuts at: 2356 2981 2356 Size: 625 5235 EcoO109I rG'GnC_Cy Cuts at: 2640 2968 2640 Size: 328 5532 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 EcoRV GAT'ATC Cuts at: 3073 3073 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2179 2922 5007 1678 Size: 501 743 2085 2531 Fragments arranged by size: 2531 2085 743 501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2650 2682 2826 2858 4501 Size: 32 135 32 1078 32 144 32 1643 Cuts at: 4501 4533 4575 4607 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1643 1078 144 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KasI G'GCGC_C Cuts at: 2918 2918 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2803 2812 4974 5158 1305 Size: 1498 9 2162 184 2007 Fragments arranged by size: 2162 2007 1498 184 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 2262 2795 2942 10 Size: 54 1425 343 327 103 533 147 2928 Fragments arranged by size: 2928 1425 533 343 327 147 103 54 MslI CAynn'nnrTG Cuts at: 518 1166 3149 3818 4177 4336 518 Size: 648 1983 669 359 159 2042 Fragments arranged by size: 2042 1983 669 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2309 2897 3276 3953 5101 5346 1606 Size: 703 588 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 703 677 588 379 245 NaeI GCC'GGC Cuts at: 2088 2088 Size: 5860 NarI GG'CG_CC Cuts at: 2919 2919 Size: 5860 NcoI C'CATG_G Cuts at: 513 1065 3123 513 Size: 552 2058 3250 Fragments arranged by size: 3250 2058 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NgoMIV G'CCGG_C Cuts at: 2086 2086 Size: 5860 NheI G'CTAG_C Cuts at: 1052 2443 1052 Size: 1391 4469 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 2574 2574 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2724 1613 Size: 1111 4749 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2640 2640 Size: 5860 PstI C_TGCA'G Cuts at: 830 2965 3212 830 Size: 2135 247 3478 Fragments arranged by size: 3478 2135 247 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2309 2309 Size: 5860 SacII CC_GC'GG Cuts at: 2898 2898 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2392 2817 2961 3208 4343 5024 5215 Size: 219 1347 425 144 247 1135 681 191 Cuts at: 5215 826 Size: 1471 Fragments arranged by size: 1471 1347 1135 681 425 247 219 191 144 SfoI GGC'GCC Cuts at: 2920 2920 Size: 5860 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 2772 1517 Size: 1255 4605 StyI C'CwwG_G Cuts at: 513 1065 2179 3123 3299 513 Size: 552 1114 944 176 3074 Fragments arranged by size: 3074 1114 944 552 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2781 2960 3887 3904 4057 4242 4687 Size: 1255 1201 179 927 17 153 185 445 Cuts at: 4687 325 Size: 1498 Fragments arranged by size: 1498 1255 1201 927 445 185 179 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4106 5818 96 Size: 274 80 33 51 1443 2129 1712 138 Fragments arranged by size: 2129 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2684 2716 5820 5852 2684 Size: 32 3104 32 2692 Fragments arranged by size: 3104 2692 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2664 1316 Size: 1348 4512 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 2253 2583 3096 3989 2253 Size: 330 513 893 4124 Fragments arranged by size: 4124 893 513 330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI